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Öğe 1H magnetic resonance spectroscopy of the normal testis(Elsevier Science Inc, 2008) Firat, Ahmet Kemal; Ugras, Murat; Karakas, Hakk M.; Erdem, Gulnur; Kurus, Meltem; Ugras, Meltem; Celik, TayfunPurpose: The purpose of this study was to determine the pre- and postpubertal H-1 magnetic resonance spectroscopic characteristics of the normal testis to establish baseline values for further clinical studies. Materials and Methods: The subjects consisted of male volunteers, of whom 19 were prepubertal with ages between 7 and 13 years and 24 were postpubertal with ages between 19 and 39 years. Their testes were evaluated at 1.5 T with magnetic resonance spectroscopy; in addition, testis volumes were measured. Major metabolite peaks were identified and their ratios were calculated. Metabolite differences of testis between pre- and postpubertal age were analyzed. Results: Major constituents of spectra were 3.21 ppm choline and 0.9-1.3 ppm lipid peaks. At the echo time (TE) spectrum of 31 ms, choline/lipid ratios ranged from 0.35 to 8.30 (mean=1.87) in postpubertal males and from 0.06 to 5.45 (mean=0.88) in prepubertal males (P<.013). At the TE spectrum of 136 ms, choline/lipid ratios ranged from 0.66 to 15.42 (mean=4.09) in postpubertal males and from 0.05 to 4.91 (mean=0.9) in prepubertal males (P<.016). Conclusions: Choline/lipid ratio was higher in the postpubertal period. The existence of higher choline peak in that age group should be due to the initiation of spermatogenesis. The decrease in the lipid peak may represent the effect of testosterone on testicular tissue or may be due to histochemical changes initiated by puberty. The significant decrease in choline/lipid ratio noted after puberty could represent the presence of spermatogenesis. This hypothesis should be evaluated by further studies on postpubertal. subjects with impaired spermatogenesis. (C) 2008 Published by Elsevier Inc.Öğe Apricot ameliorates alcohol induced testicular damage in rat model(Pergamon-Elsevier Science Ltd, 2009) Kurus, Meltem; Ugras, Murat; Ates, Burhan; Otlu, AliIn this study, we intended to determine the possible preventive effects of dietary apricot on oxidative stress due to ethanol usage in rat testes. The animals were divided into six groups as follows: Group 1 was control. Group 2 received ethanol. Group 3 were fed with apricot diet for 3 months. Group 4 were fed with apricot diet for 6 months. Group 5 received ethanol and apricot diet for 3 months. Group 6 were fed apricot diet for 3 months, and then ethanol + apricot diet for 3 months. Following sacrification, the testes were treated for morphological (tubular and germ cell histology, Sertoli and Leydig cell counts) and biochemical (superoxide dismutase, glutathion peroxidase, catalase, malondialdehyde) analyses. In Group 2, severe histopathological changes in seminiferous tubules and germ cells were determined as well as tubular degeneration and atrophy. Sertoli and Leydig cell counts in the interstitial tissue were decreased. Biochemical parameters revealed tissue oxidative stress. Similar alterations existed in Group 5, although to a lesser extent. In Groups 1, 3 and 4, no histopathological alterations were noted. Results of Group 6 were similar to the controls. Apricot rich diet may have a preventive role on histopathological changes caused by alcohol in rat testes.(C) 2009 Elsevier Ltd. Ail rights reserved.Öğe The effect of resveratrol in tracheal tissue of rats exposed to cigarette smoke(Taylor & Francis Ltd, 2009) Kurus, Meltem; Firat, Yezdan; Cetin, Asli; Kelles, Mehmet; Otlu, AliObjective: The aim of this study was to evaluate the effect of resveratrol on the tracheal tissue of rats exposed to cigarette smoke. Materials and methods: 40 adult Wistar albino rats were divided into four groups for an experiment of 6 weeks. Animals in group 1 were controls (n = 10). Rats in group 2 were exposed to cigarette smoke only, and rats in group 3 received daily intraperitoneal injections of resveratrol (10 mg/kg/d). Animals in group 4 were exposed to both cigarette smoke and intraperitoneal injections of resveratrol. Rats of all groups were sacrificed using cervical dislocation. The tracheas were removed and embedded in paraffin blocks. Sections of 4-5 mu m thickness were prepared from the blocks. These sections were stained with hematoxylin and eosin, periodic acid-Schiff, and Alcian blue and viewed with a Leica DFC 280 light microscope. Results: Tracheal sections showed that, in group 2 (cigarette smoke group), there was desquamation of epithelial cells into the tracheal lumen, loss of cilia in the epithelial layer, an increase of goblet cells, activation of serous glands at the submucosa, and cell infiltration. In group 4 (cigarette smoke + resveratrol group), all these findings also existed but only a few sections were affected. It was observed that cigarette smoking caused morphological changes such as epithelial degeneration in the upper airway. These morphological changes were correlated with the amount of toxic substances in the cigarette smoke. Conclusion: We found that resveratrol had a preventive role in the histopathological changes caused by cigarette smoking in the rat trachea.Öğe Effect of resveratrol on tubular damage and interstitial fibrosis in kidneys of rats exposed to cigarette smoke(Sage Publications Inc, 2009) Kurus, Meltem; Ugras, Murat; Esrefoglu, MukaddesThe aim of this study was to evaluate the effect of resveratrol on kidney tissue of rats exposed to cigarette smoke. Forty adult male Wistar Albino rats were divided into four groups. Animals in group 1 was the control group. For 6 weeks, group 2 was exposed to cigarette smoke; group 3 received daily intraperitoneal injections of resveratrol (10 mg/kg/d); and group 4 was exposed to both cigarette smoke and intraperitoneal resveratrol. All rats were sacrificed with cervical dislocation. The kidney tissues were obtained, fixed in Bouin's fixative and embeded in paraffin blocks. Samples were sectioned to 4-5 microns thickness, stained with hematoxylin/eosin (H/E), Masson's trichromic, periodic acid-schiff (PAS) and were examined by light microscopy for tubular injury and interstitial fibrosis. Results were compared by non-parametric tests. Hydropic degeneration, tubular atrophy, tubulo-interstitial fibrosis, interstitial cell infiltration, vacuolar degeneration and desquamation were prominent in group 2. In group 4, hydropic degeneration, epithelial cell vacuolization and desquamation was not observed, but occasional tubular atrophy and dilation were observed. Our study suggests that, some morphological alterations in the rat kidney, due to cigarette smoke may be prevented by resveratrol.Öğe Evaluation of antioxidant effect of resveratrol on testicular tissue in rats that were exposed to cigarette smoke(Aves, 2011) Soylemez, Haluk; Ugras, Yahya Murat; Beytur, Ali; Oguz, Fatih; Kurus, Meltem; Karabulut, Aysun BayObjective: The aim of this study was to investigate the negative effects of cigarette on testicular biochemistry and histology of rats and to evaluate if resveratrol could alter these effects. Materials and methods: Thirty-two male Wistar Albino rats were divided into 4 grups for an experiment of 6 weeks. Group 1 was used as control; Group 2 was only exposed to cigarette smoke. Group 3 only received intraperitoneal injections of resveratrol (10 mg/kg/day). Group 4 was exposed to both cigarette smoke and resveratrol (10 mg/kg/day). At the end of the experimental period, the rats were sacrified and testes of all rats were removed. Malondialdehyde (MDA), glutathione (GSH), and nitric oxide (NO) levels were measured on testicular tissue and histopathologic examination was performed with light microscope. Results: Weight follow-up of rats revealed no significant difference among groups. MDA level was lower in cigarette smoke and resveratrol group than that of only cigarette smoke group. GSH level showed significant decrease in only cigarette smoke group, while GSH level increased in cigarette smoke and resveratrol group. Differences in NO levels were not statisticaly significant among groups. The histological evaluation revealed that Johnsen score which indicates spermatogenesis was significantly lower only in cigarette smoke rats than all other groups. Conclusion: Oxidative stress develops in testis of rats that were exposed to cigarette smoke, and resveratrol has preventive effects on this damage with direct or indirect antioxidant activity.Öğe An Experimental Study of the Histopathological Effects of Melatonin on Cyclosporin Induced Lung Damage(Bilimsel Tip Publishing House, 2008) Kurus, Meltem; Esrefoglu, Mukaddes; Otlu, AliIn recent studies, it has been reported that the widely used immunosuppressive agent, cyclosporine A (CyA), causes tissue damage and that free oxygen radicals play a role in this damage. Melatonin, which is the most important indoleamine released by the pineal gland, is a free radical scavenger and an antioxidant agent. In this study, we aimed to study histopathologically the probable positive effects of Melatonin on CyA induced lung tissue damage. Four groups, each with 8 rats, were used in this study: Group 1; control, Group 2; 4 mg/kg/day intraperitoneal (i.p.) melatonin, Group 3; 10 mg/kg/day subcutaneous (s.c.) CyA and Group 4; 4 mg/kg/day melatonin (i.p.) plus 10 mg/kg/day CyA (s.c.). The study lasted for 28 days for each group. At the end of this period, the rats were killed with lethal anesthesia. Their lungs were removed and embedded in paraffin blocks before being processed for microtome. The preparations were stained with Haematoxylene- Eosin (H-E), and Masson's trichrome dyes. Both control and Melatonin groups appeared normal. In the CyA group, congestion of the parenchyma,perialveolar edema, perivascular and peribronchial infiltration and thickening of interalveolar septum as a result of an increase in connective tissue were observed in the rat lungs. In the CyA plus melatonin group, histopathological findings were significantly milder than those of the CyA group. Furthermore, mild congestion and edema was encountered only in rare areas. It was concluded that CyA dependent damage may be reversible and that this damage may be significantly decreased by melatonin administration.Öğe Granulosa cell and oocyte mitochondrial abnormalities in a mouse model of fragile X primary ovarian insufficiency(Oxford Univ Press, 2016) Dioguardi, Carola Conca; Uslu, Bahar; Haynes, Monique; Kurus, Meltem; Gul, Mehmet; Miao, De-Qiang; De Santis, LuciaSTUDY HYPOTHESIS: We hypothesized that the mitochondria of granulosa cells (GC) and/or oocytes might be abnormal in a mouse model of fragile X premutation (FXPM). STUDY FINDING: Mice heterozygous and homozygous for the FXPM have increased death (atresia) of large ovarian follicles, fewer corpora lutea with a gene dosage effect manifesting in decreased litter size(s). Furthermore, granulosa cells (GC) and oocytes of FXPM mice have decreased mitochondrial content, structurally abnormal mitochondria, and reduced expression of critical mitochondrial genes. Because this mouse allele produces the mutant Fragile X mental retardation 1 (Fmr1) transcript and reduced levels of wild-type (WT) Fmr1 protein (FMRP), but does not produce a Repeat Associated Non-ATG Translation (RAN)-translation product, our data lend support to the idea that Fmr1 mRNA with large numbers of CGG-repeats is intrinsically deleterious in the ovary. WHAT IS KNOWN ALREADY: Mitochondrial dysfunction has been detected in somatic cells of human and mouse FXPM carriers and mitochondria are essential for oogenesis and ovarian follicle development, FX-associated primary ovarian insufficiency (FXPOI) is seen in women with FXPM alleles. These alleles have 55-200 CGG repeats in the 5' UTR of an X-linked gene known as FMR1. The molecular basis of the pathology seen in this disorder is unclear but is thought to involve either some deleterious consequence of overexpression of RNA with long CGG-repeat tracts or of the generation of a repeat-associated non-AUG translation (RAN translation) product that is toxic. STUDY DESIGN, SAMPLES/MATERIALS, METHODS: Analysis of ovarian function in a knock-in FXPM mouse model carrying 130 CGG repeats was performed as follows on WT, PM/+, and PM/PM genotypes. Histomorphometric assessment of follicle and corpora lutea numbers in ovaries from 8-month-old mice was executed, along with litter size analysis. Mitochondrial DNA copy number was quantified in oocytes and GC using quantitative PCR, and cumulus granulosa mitochondrial content was measured by flow cytometric analysis after staining of cells with Mitotracker dye. Transmission electron micrographs were prepared of GC within small growing follicles and mitochondrial architecture was compared. Quantitative RT-PCR analysis of key genes involved in mitochondrial structure and recycling was performed. MAIN RESULTS AND THE ROLE OF CHANCE: A defect was found in follicle survival at the large antral stage in PM/+ and PM/PM mice. Litter size was significantly decreased in PM/PM mice, and corpora lutea were significantly reduced in mice of both mutant genotypes. Mitochondrial DNA copy number was significantly decreased in GC and metaphase II eggs in mutants. Flow cytometric analysis revealed that PM/+ and PM/PM animals lack the cumulus GC that harbor the greatest mitochondrial content as found in wild-type animals. Electron microscopic evaluation of GC of small growing follicles revealed mitochondrial structural abnormalities, including disorganized and vacuolar cristae. Finally, aberrant mitochondrial gene expression was detected. Mitofusin 2 (Mfn2) and Optic atrophy 1 (Opa1), genes involved in mitochondrial fusion and structure, respectively, were significantly decreased in whole ovaries of both mutant genotypes. Mitochondrial fission factor 1 (Mff1) was significantly decreased in PM/+ and PM/PM GC and eggs compared with wild-type controls. LIMITATIONS, REASONS FOR CAUTION: Data from the mouse model used for these studies should be viewed with some caution when considering parallels to the human FXPOI condition. WIDER IMPLICATIONS OF THE FINDINGS: Our data lend support to the idea that Fmr1 mRNA with large numbers of CGG-repeats is intrinsically deleterious in the ovary. FXPM disease states, including FXPOI, may share mitochondrial dysfunction as a common underlying mechanism. LARGE SCALE DATA: Not applicable.Öğe The Histopathological Effect of Resveratrol in Thyroid Tissue of Rats Exposed to Cigarette Smoke(Ortadogu Ad Pres & Publ Co, 2009) Kurus, Meltem; Sogutlu, Gokhan; Firat, Yezdan; Esrefoglu, Mukaddes; Yologlu, Saim; Ozturk, Feral; Otlu, AliObjective: The aim of this study was to assess the histopathological effect of resveratrol in thyroid tissue of rats exposed to cigarette smoke. Material and Methods: Forty adult, male Wistar Albino rats were divided into four groups for an experiment of 6 weeks duration. Group I was the control group. Rats in group 2 were exposed to cigarette smoke only and rats in group 3 received daily intraperitoneal injections of resveratrol (10 mg/kg/d). Animals in group 4 were exposed to both cigarette smoke and intraperitoneal injections of resveratrol. Rats of all groups were sacrificed; their thyroid glands were removed and were examined histopathologically. Results: While the control group and the resveratrol group had normal thyroid tissue, in the group exposed to cigarette smoke there was a significant decrease in follicles and differentiation of epithelial cells from cubic to flat cells. There was intracytoplasmic vacuolization in some epithelial cells, irregularity in follicular cells decreasing area and cell infiltrations. On the other hand, we observed significant improvement in these histopathological differences in the group that was exposed to both cigarette smoke and resveratrol. Conclusion: Resveratrol has healing effects on the damage of thyroid tissue of rats that are exposed to cigarette smoke at a dose and duration tested in this study.Öğe Melatonin Prevents Cyclosporine-Induced Hepatotoxicity in Rats(Karger, 2009) Kurus, Meltem; Esrefoglu, Mukaddes; Sogutlu, Gokhan; Atasever, AlperObjectives: Cyclosporine A (CsA) is a widely used immunosuppressive agent that is implicated in the formation of free oxygen radicals. Melatonin is known to be a free radical scavenger and an antioxidant agent. This study was designed to investigate the effects of melatonin on CsA-induced liver damage by histopathological examination. Materials and Methods: Thirty-two male rats of Sprague-Dawley origin were divided into 4 groups of 8 and treated for 28 days as follows: group 1 received daily doses of 0.1 ml/kg olive oil s.c.; group 2 received 4 mg/kg of melatonin; group 3 received 10 mg/kg CsA diluted in 0.1 ml/kg olive oil; group 4 was treated with 4 mg/kg melatonin i.p. and 10 mg/kg CsA s.c. Finally, the rats were sacrificed by terminal anesthesia, and liver tissue specimens were processed for light microscopy, stained with HE and examined under a light microscope. Results: Specimens of the control group showed normal liver histology, whereas group 3 showed major histopathological changes, such as cytoplasmic vacuolization, dilatation of the sinusoids, apoptosis and many mitotic figures. In group 4, the normal histology of the liver was preserved, although apoptosis, mitotic figures and cytoplasmic vacuolization were still infrequently observed. Nevertheless, there were significant differences between group 2 (melatonin) and group 3 (CsA) and between group 3 (CsA) and group 4 (CsA + melatonin) concerning these 3 parameters (vacuolization, sinusoidal dilatation and apoptosis). Conclusion: The results of this study suggest that CsA-related liver toxicity in rats could be significantly reduced by melatonin administration. Copyright (C) 2009 S. Karger AG, BaselÖğe The Morphological and Numeral Changes of Goblet Cells in the Jejunum of Ischemia-Reperfusion Applied Rats(Ortadogu Ad Pres & Publ Co, 2010) Dag, Meral; Kurus, Meltem; Sogutlu, Gokhan; Estegoglu, Mukaddes; Ozturk, Feral; Yologlu, Saim; Otlu, AliObjective: In this study, we aimed to investigate the numeric and morphological changes of goblet cells after experimental ischemia and reperfusion damage. Material and Methods: In our study, male Sprague Dawley rats were randomly divided into four groups being eight rats in each group. Groups were: control, sham, ischemia (15 minutes) and ischemia-reperfusion groups (I/R, 15 min. I/15 min.R). After the experiment, all rats were sacrificed and their jejunums were taken out. All the necessary procedures were carried out for morphological evaluation. Results: In the ischemia group, villi morphological changes from splitting up to atrophy, and were shorter; goblet cells were found to show reduction both in number and size. On the other hand, it was found that goblet cells in the crypts showed no change in number but in size. Findings in the I/R group were milder than the ischemia group, but the sizes of villi were found smaller in this group. While the number of goblet cells in the villi was normal, it was decreased in the crypts and the sizes of goblets both in the villi and in the crypts were reduced. Conclusion: We thought that during the ischemia, the number of goblets were decreased since they fall into the lumen. The rest were small because they came from the basis of the crypts; during the I/R procedure, the number of cells turned to normal because of the arrival of the goblets from the crypts to the villi. However, in this way the number in them in the crypts decreased and they looked small due to incomplete maturation.Öğe Morphological Changes and Vascular Reactivity of Rat Thoracic Aorta Twelve Months After Pinealectomy(Ortadogu Ad Pres & Publ Co, 2010) Kurcer, Zehra; Ozturk, Feral; Sahna, Engin; Kurus, Meltem; Olmez, ErcumentObjective: Melatonin, a hormone produced by the pineal gland, has been suggested to protect against development of hypertension and atherosclerosis. In this study, the effects of long-term melatonin deficiency for twelve months after pinealectomy on the alpha-adrenergic-contractions induced by phenylephrine, endothelium-dependent relaxation responses to acetylcholine and the morphological changes in the rat thoracic aorta were studied. Material and Metods: Rats were pinealectomized twelve months before the beginning of the vasomotor studies. Rings of arteries were mounted in isolated tissue baths for the measurements of isometric contractile force. The contractile responses to phenylephrine and endothelium-dependent relaxation responses to acetylcholine in the vessels were evaluated. Endothelial function was evaluated by vascular relaxation to acetylcholine. Histological examinations demonstrated the alterations of tunica media in the vessels of pinealectomized rats. Results: Thick and thin areas were observed in the transverse sections of vessels and the ratio of the widest media thickness to the narrowest was found significantly increased in pinealectomized group (2.85 +/- 056) when compared to the control group (1.65 +/- 0.10). In addition, alpha-smooth muscle actin and elastic lamellae staining of the media were attenuated in pinealectomized rats. Although contractile responses of vessels to phenylephrine in pinealectomized rats were lower than control group, significant difference was found for only one concentration (3x 10-8 mol l-1) of phenylephrine. There was no difference between the relaxation responses to acetylcholine in pinealectomized and control groups. Conclusion: These results show that long-term melatonin deficiency may cause some morphological changes in the tunics media of vessels. However, the function of endothelium and vascular responsiveness to proportional to-adrenergic stimulus seem to be mostly protected.Öğe Oral L-arginine protects against cyclosporine-induced hepatotoxicity in rats(Elsevier Gmbh, 2008) Kurus, Meltem; Esrefoglu, Mukaddes; Karabulut, Aysun Bay; Sogutlu, Gokhan; Kaya, Mine; Otlu, AliCyclosporine A (CyA) leads to liver injury, probably by causing the production of free radicals and resulting in nitric oxide (NO) deficiency. We evaluated CyA-mediated liver damage histopathologically to determine the possible beneficial effects Of L-arginine (L-Arg). In this study, 7 groups of Sprague-Dawley rats; (1) Control group; (2) 0.9% NaCl group; (3) CyA group: 7.5 mg/kg/day; (4) L-Arg group: 2 g/It/day; (5) L-NAME (N-nitro-L-arginine methyl ester) group: 5mg/100ml/day; (6) CyA+L-Arg group: L-Arg (2 g/It/day) + CyA (7.5mg/kg/day); and (7) CyA + L-NAME group: CyA (7.5mg/kg/day) + L-NAME (5mg/100ml/day) were included. At the end of the treatments, animals were killed and hepatic tissues were treated for morphological (hematoxylin and eosin) and biochemical (NO and malondialdehyde, NIDA) analyses, and serum was processed for biochemical (alanine transaminase (ALT), aspartate transaminase (AST), bilirubin, alkaline phosphatase (ALP) and total protein) study. The results indicated that CyA-induced hepatotoxicity was characterized by sinusoidal dilatation, hepatocellular vacuolization, neutrophilic infiltration and hepatocellular necrosis. These findings were less pronounced in the CyA + L-Arg group than CyA alone group. L-NAME group showed moderate changes. The CyA + L-NAME (Group 7) had more severe changes. We found changes in tissue NO and NIDA levels. We think that the tissue damage caused by CyA is mild and reversible at the period when biochemical parameters are just starting to become abnormal and that L-Arg may have a protective effect against CyA damage on liver. (C) 2008 Elsevier GmbH. All rights reserved.Öğe Protective effect of oral L-arginine supplementation on cyclosporine induced nephropathy in rats(Springer, 2005) Kurus, Meltem; Esrefoglu, Mukaddes; Bay, Aysun; Ozturk, FeralBackground: One of the major adverse effects of long term cyclosporine A ( CyA) administration is chronic nephrotoxicity. Several studies have suggested that alterations of the L-arginine (L-Arg) nitric oxide ( NO) pathway may be involved in the pathogenesis of CyA-induced kidney damage. Aim: We postulated that in vivo activation of L-Arg-NO pathway might have a beneficial effect on CyA-induced renal damage. Conditions of chronic NO enhancement was established with L-Arg supplementation and chronic NO blockade with N-nitro-L-Arg methyl ester ( L- NAME). We tested the hypothesis that, if CyA administration alters intrarenal NO synthesis, then exogenous L-Arg supplementation could limit renal injury, on the contrary, L- NAME, a potent competitive inhibitor of NO synthesis, could enhance CyA nephrotoxicity. Harmful effect of NO blockade indirectly supports the beneficial effect of NO in a model of CyA nephrotoxicity. Methods: Rats were administered vehicle (VH), CyA (7.5 mg/kg/day), CyA + L-Arg (2g/kg/day), CyA + L- NAME (5 mg/100ml/day), CyA + L-Arg + L- NAME, VH + L-Arg, VH + L-NAME and were sacrificed at the end of the experiment. Body weight, serum creatinine, blood urea nitrogen ( BUN) and NO levels were determined. Tubular injury and interstitial fibrosis were evaluated semiquantitatively using scoring systems on paraffin sections stained with hematoxylin/eosin (H/E), Masson's trichromic and periodic acid-Schiff (PAS). Results: The CyA group developed marked renal injury, characterized by a significant increase in serum creatinine and BUN, and histopathological alterations including tubular dilatation, vacuolization, necrosis, interstitial cell infiltration and tubulointerstitial fibrosis. CyA reduced serum NO level. L-Arg treatment significantly enhanced NO biosynthesis and protected animals from CyA-induced kidney damage. In contrast L- NAME strikingly reduced serum NO level, and worsened biochemical and histopathological alterations. Conclusion: Chronic CyA nephrotoxicity can be aggravated by NO blockade and ameliorated by NO enhancement suggesting that L-Arg supplementation may be protective in CyA nephrotoxicity.Öğe Protective effects of prunus armeniaca l (apricot) on low dose radiation-ınduced kidney damage in rats(Türk Nefroloji Diyaliz ve Transplantasyon Dergisi, 2014) Kurus, Meltem; Elbe, Hülya; Otlu, Ali; Taslıdere, Elif; Uğraş, MuratÖz: AMAÇ: Bu çalışmada amacımız, antioksidan etkiye sahip Prunus armeniaca L (kayısı) nın böbrek dokusu üzerine radyoprotektif etkisinin histopatolojik yöntemlerle araştırılmasıdır. GEREÇ ve YÖNTEMLER: Çalışmamızda 10 ar adet Sprague Dawley cinsi sıçanlardan oluşan 6 grup kullanılmıştır. Kontrol (normal diyet) grubu: 28 hafta normal diyetle beslenen grup. Normal diyet+rad grubu: 20 hafta boyunca normal diyet alıp son 8 hafta normal diyet+ radyasyon alan grup. Kayısı diyeti alan grup: 28 hafta boyunca kayısı diyeti ile beslenen grup. Kayısı diyeti+Rad grubu: 20 hafta boyunca kayısı diyeti alıp son 8 hafta kayısı diyeti+radyasyon alan grup. Kayısı diyeti+normal diyet alan grup: 20 hafta boyunca kayısı diyeti alıp devam eden 8 hafta boyunca normal diyet alan grup. Rad+Kayısı diyeti alan grup: 20 hafta boyunca kayısı diyeti alıp devam eden 8 hafta boyunca normal diyet+ radyasyon alan grup. BULGULAR: Çalışmamızda, kontrol gruplarında normal histolojik yapı mevcutken, radyasyon grubunda glomerüler hasar, hemoraji, intertisyel fibrozis ve infiltrasyon gibi histopatolojik değişikliklere rastlanmıştır. Diğer taraftan, Kayısı diyeti+Rad grubu ve Rad+Kayısı diyeti alan gruplarda bu dejenerasyonların azaldığı tespit edilmiştir. SONUÇ: Sonuç olarak yaptığımız çalışmada radyasyona maruz kalındığında kayısı katkılı beslenmenin böbrek üzerine radyasyonun verdiği hasarı azalttığı tespit edilmiştir.Öğe Prunus armeniaca L (apricot) protects rat testes from detrimental effects of low-dose x-rays(Pergamon-Elsevier Science Ltd, 2010) Ugras, Murat Y.; Kurus, Meltem; Ates, Burhan; Soylemez, Haluk; Otlu, Ali; Yilmaz, IsmetExposure to low x-ray doses damages the spermatozoa, mainly by late-onset (ie, after 3 months) oxidative stress. Antioxidants ameliorate oxidation and prevent tissue damage. Prunus armeniaca L (apricot), rich in carotenoids and vitamins, is a potent natural antioxidant. We hypothesized that an apricot-rich diet might ameliorate the detrimental effects of low-dose x-rays on testis tissue. A 20% apricot diet was composed isoenergetically to the regular rodent diet. The total phenolic content, reducing power, and antioxidant capacity of both diets were determined. Sprague-Dawley rats received apricot-rich diets before and after x-ray exposure. Regular diets were given to controls. Rats were exposed to 0.2 Gy x-rays at the eighth week and were euthanized at the 20th postexposure week. Testicular oxidative status was determined by tissue thiobarbituric acid-reactive substances, reduced glutathione, superoxide dismutase, and catalase activities. For histologic evaluation, qualitative and quantitative microscopic determinations were performed, and Leydig and Sertoli cell counts and Johnsen scores were measured. The control diet group had significant testicular oxidative stress and mild tissue deterioration. Leydig and Sertoli cell counts, tubule diameters, and Johnsen scores were significantly decreased in the exposure groups. Apricot-rich diet significantly ameliorated the oxidative status and prevented the damage in tubular histology. The protective effects were prominent when the diet was maintained throughout the time course and were partially protected when the diet was initiated after exposure. The natural antioxidant activity of apricot ameliorates the delayed detrimental effects of low-dose irradiation on testis tissue. The high total antioxidant capacity of the apricot deserves further investigation. (C) 2010 Elsevier Inc. All rights reserved.
