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    The Cytotoxicity Profile, Apoptosis Mechanism, and Molecular Docking Studies of a Series of Benzimidazolium Derivative Morpholine-Substituted Ag(I) Heterocyclic Carbene Complexes
    (Springer, 2023) Kutlu, Tuerkan; Yildirim, Isil; Dikmen, Miris; Tok, Tugba Taskin; Aktas, Aydin; Gok, Yetkin
    The main problems experienced in treatment with anticancer drugs are undesirable side effects, and toxicity. Minimal side effects for new anticancer compounds may be met due to enhanced efforts to clarify the compound's mechanisms of action. Therefore, we aimed to investigate whether or the cytotoxic effect and apoptosis mechanism of a series Ag(I)NHC complexes on non-small cell lung cancer cell line (A549) and normal lung fibroblast cell line (CCD-19Lu) in this study. The cytotoxicity was determined by using the MTT method, and apoptotic effects were detected by cell cycle, annexin-V/propidium iodide (PI) staining and cell cycle, caspase-3, mitochondrial membrane potential analysis. Molecular docking studies were performed using in silico ADMET analysis, and molecular docking information on the compounds was gained using the DS 3.5 software subprotocol. All the time, the cytotoxic effect of silver compounds was monitored for 24 h in comparison to cisplatin. The apoptotic effect of these compounds increased in cancer cells as compared to normal cells. Complex 3b exhibited the highest cytotoxic activity on cancer cell in 24 and 72 h, but complex 3a exhibited the highest cytotoxic activity on cancer cell s in 48 h. Moreover, all Ag(I)NHC complexes exhibited significant statistical difference depending on the increase in concentration on cancer cells, and all compounds induced apoptosis associated with distributing of membrane polarization and stopping the cell cycle in phase G1 and the caspase-3 activity. Caspase-3 activity of the new Ag(I)NHC compounds showed 8.3 to 17.6-fold increase compared the untreated cells. The loss of mitochondrial membrane potential indicated that JC-1 assay results were 16.9 to17.2-fold higher than normal cells in Ag(I)NHC compounds and 11.3-fold higher her in cisplatin. In addition, molecular docking studies were executed on the Ag(I)NHC complexes, and cisplatin estimate that the binding modes towards the EGFR kinase. Because epidermal growth factor receptor (EGFR) is expressed highly in a great number of epithelial tumors. These findings suggested that Ag(I)NHC complexes exhibited anticancer activity and may be considered to have a new therapeutic potential for human non-small cell lung cancer cell treatment.
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    Protective effect of dietary apricot kernel oil supplementation on cholesterol levels and antioxidant status of liver in hypercholesteremic rats
    (Wfl Publ, 2009) Kutlu, Tuerkan; Durmaz, Goekhan; Ates, Burhan; Erdogan, Ali
    Apricot kernel oil is a rich source of MUFA and PUFA, including mainly oleic (about 70%) and linoleic acids, respectively. In addition, apricot kernel oil could be considered as a good source of bioactive compounds such as tocopherols and phytosterols consisting mainly of they gamma-isomer and beta-sitosterol, respectively. Given to its high content of oleic acid, apricot kernel oil is considered as a healthy supplement in diet. In the present study, we investigated the effects of apricot kernel oil supplementation on cholesterol and malondialdehyde (MDA) levels and glutathione peroxidase (GPx) and catalase (CAT) activity in hypercholesteremic rats. Hypercholesteremia was produced by feeding rats with a semisynthetic diet that contained high cholesterol and cholic acid. A high cholesterol diet caused a decrease in CAT and GPx activity, while apricot kernel oil caused a significant activity increase of these enzymes (P < 0.05). The group fed with apricot kernel oil supplementation showed higher enzyme activities than sunflower oil groups irrespective of cholesterol (P < 0.05). Results of the present study indicate that apricot kernel oil causes improvement in liver antioxidant status of rats in comparison to sunflower oil which is a commonly consumed vegetable oil.
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    Roasting-Related Changes in Oxidative Stability and Antioxidant Capacity of Apricot Kernel Oil
    (Wiley, 2010) Durmaz, Goekhan; Karabulut, Ihsan; Topcu, Ali; Asilturk, Meltem; Kutlu, Tuerkan
    Apricot kernels were roasted for various lengths of time (0-30 min) at 180 A degrees C and changes in the oxidative stability, antioxidant capacity, color, as well as the level of tocopherols and fatty acids of the apricot kernel oil (AKO) were monitored. While the level of tocopherols decreased, the oxidative stability and antioxidant capacity of AKO increased with roasting, probably due to the formation of antioxidative Maillard reaction products (MRPs) during the roasting. Medium roasted samples (15-20 min) were found to be more resistant to oxidative deterioration. The oil from the 30-min roasted sample was more susceptible to oxidation compared to the oil from the 20-min roasted sample in most of the stability tests. Relatively shorter roasting periods (5-10 min) also led to a decrease in oxidative stability in comparison to the unroasted sample. Brownish color and antiradical activity increased with roasting and the highest values were measured in the 30 min roasted sample.
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    Some Physical and Chemical Changes during Fruit Development of Five Common Apricot (Prunus armeniaca L.) Cultivars
    (Japanese Soc Food Sci & Technology, 2010) Durmaz, Goekhan; Cam, Mustafa; Kutlu, Tuerkan; Hisil, Yasar
    Five apricot (Prunus armeniaca L.) cultivars were examined throughout fruit development period to monitor the changes in quality characteristics including hardness, Brix, color, titratable acidity and pH. Changes in the amount of sugars (fructose, glucose and sucrose) and organic acids (citric, malic and quinic) were also determined by HPLC. The predominant sugar of cultivars found to be glucose at the initial ripening stages; however, sucrose or glucose became predominant at the end of fruit maturation. Sugar/acid rate was found to be the highest in Hasanbey (HB) cultivar during unripe period, while Kabaasi (KA) showed the highest value at full ripeness. Low soluble solid containig varieties of Zerdali (ZD) and HB reached fruit maturity earlier than the other cultivars. Soganci (SO) measured as the hardest fruit at maturity while ZD was the softest cultivar. Chroma as a measure of color, was found to be well corelated with maturity level rather than L value. In the course of ripening, total acid content decreased along with the increasing amount of total sugar for all cultivars.