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    Comparison of the methods used for the detection of methicillin resistance in Staphylococci
    (2002) Kuzucu Ç.; Dalgalar M.; Durmaz R.; Dikerel Ş.
    In this study, the methicillin resistance of 112 Staphylococcus aureus and 93 coagulase negative Staphylococcus (CNS) strains, which were initially found methicillin resistant by routine disk diffusion method in our laboratory, have been searched by microdilution and oxacillin agar screen test and the results were compared with the results obtained by disk diffusion method. The presence of mecA gene was investigated by polymerase chain reaction in case of discordant results. All S.aureus strains (100%) and 69.9% (65/93) of CNS strains were found resistant to methicillin by three of the methods. Of CNS isolates, 28 strains which were found methicillin resistant by disk diffusion method, were found methicillin susceptible by oxacillin agar screen method, and 27 of these were detected as mecA positive. Our results indicated that, the three methods tested were reliable for the detection of methicillin resistance in S.aureus strains, but oxacillin agar screen revealed to be unsatisfactory for the detection of methicillin resistance in CNS.
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    Distribution of bacteria isolated from urine cultures in Malatya University Hospital Laboratory
    (2006) Yetkin G.; Kuzucu Ç.; Güçlüer N.
    The aim of this letter was to report the distribution of bacterial species isolated from the urine samples of patients in Malatya, which is located in Estern Anatolia part of Turkey. A total of 3.774 urine cultures were performed in the period of April-June 2006, and 792 (21%) of them yielded bacterial growth. The isolates have been identified by conventional methods and confirmed by Phoenix 100 (Becton-Dickinson) system. As a result, 702 (89%) Gram negative and 90 (11%) Gram positive bacteria were isolated from the samples. The most frequently isolated bacteria were Escherichia coli (58%), followed by Klebsiella spp. (14%), Pseudomonas spp. (6.4%), Enterococcus spp. (5%), Staphylococcus spp. (3.8%) and Streptococcus spp. (1.7%). The species distribution was found as follows; K.pneumoniae ssp pneumoniae (95/110), P.aeruginosa (48/51), E.faecalis (27/40), E.cloacae (19/29), P.mirabilis (19/22), C.freundii (8/12), coagulase negative staphylococci (19/30) and S.aureus (11/30). The first three array were shared by E.coli, Klebsiella spp. and Pseudomonas spp. for the samples of both outpatients and inpatients, while Pseudomonas spp. and E.coli were the most frequently isolated bacteria from the urine samples of intensive care unit patients. Our data was found parallel to the results of other national and international studies.