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    Implications of FAD Electrode Reaction Kinetics for Electrocatalysis of NADH Oxidation and Development of NAD-Dependent Enzyme Electrodes
    (Wiley-V C H Verlag Gmbh, 2014) Emre, Fatma Bilge; Lopes, Paula; Ferapontova, Elena E.
    FAD entrapped into the polyethyleneimine (PEI) matrix underwent 2e(-)/2H(+) redox transformation, in contrast to the 1e(-)/1H(+) reaction observed with non-stabilized FAD-modified electrodes. Under conditions of the 2e(-)/2H(+) reaction, FAD/PEI-modified electrodes catalytically oxidized NADH starting from -100 mV vs. Ag/AgCl. These electrodes may be used for construction of NAD-dependent dehydrogenase electrodes for clinically important analytes such as glucose when coupled to the model enzyme glucose dehydrogenase. Latter allows detection of glucose starting from -100 mV within 0.05-5 mM concentration range. These results suggest the FAD stabilization by different matrices as a simple and promising approach to achieve its 2e(-)/2H(+) redox chemistry resulting in catalysis of NADH oxidation.