Yazar "Nisari, Mehtap" seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • Küçük Resim Yok
    Öğe
    Effect of angiostatin on 1,2-dimethylhydrazine-induced colon cancer in mice
    (Sage Publications Inc, 2013) Ertekin, Tolga; Ekinci, Nihat; Karaca, Omur; Nisari, Mehtap; Canoz, Ozlem; Ulger, Harun
    Antiangiogenic therapy is supposed to be an attractive approach for antitumor treatment. Human plasminogen-derived angiostatin K1-3 is one of the most potent antiangiogenic agents known currently. However, it is unclear whether angiostatin has got protective effects on colon cancer. So we investigated the protective effects of angiostatin on 1,2-dimethylhydrazine (DMH)-induced colon cancer in mice. Thirty Balb/C male mice, weighing 25-30g and 8 weeks of age, were used. Twenty of the mice were treated with DMH subcutaneously (20mg/kg) once a week for 12 weeks. Six mice died during the DMH injection and surviving mice were divided into two groups (7 mice in DMH and 7 mice in DMH+angiostatin groups). In the angiostatin group, 6 weeks after the last DMH injection the animals were first treated with angiostatin (20g/mouse) intraperitoneally and then subcutaneously every 48h (5g/mouse) throughout a period of 12 weeks. The animals were killed after 30 weeks for histopathological examination. When we look at the distribution of lesions in the colon, they mainly occurred in the distal colon. The incidence of mean colonic lesions in a tumor-bearing mouse was 9.85 +/- 4.91 in those treated with DMH and 8.71 +/- 3.49 in those treated with angiostatin. The incidence of colon tumors was not significantly affected by low dose of angiostatin, and we noticed that the number of lesions decreased by 12% in DMH+angiostatin group compared to the number of the lesions in DMH group, but this decrease was not statistically significant (p>0.05). The administration period of angiostatin corresponds to the precancerous period and the reduction in the number of lesions could be important for the protective function of angiostatin in DMH+angiostain group. We assume that therapeutic effects of angiostatin are related to its doses, route of administration, frequency and administration period. In addition, we believe that combination of high doses of angiostatin with radiation, gene therapy or chemotherapy might be successful in proper tumor model.
  • Küçük Resim Yok
    Öğe
    Effect of carnosine on ovarian follicle in rats exposed to electromagnetic field
    (Soc Anatomica Espanola, 2022) Arslan, Ayla; Balcioglu, Esra; Nisari, Mehtap; Yalcin, Betul; Ulger, Menekse; Guler, Emel; Uzun, Gokce Bagci
    The electromagnetic field (EMF) has an effect on various organs, including the female reproductive system. The purpose of this study was to evaluate the impact of carnosine on ovarian follicle number and diameter in rats exposed to a 900 Megahertz (Mhz) electromagnetic field. In this study, six different groups were used. 40 female rats divided into groups were evaluated. The ovaries of the rats were removed at the end of the study. Routine histological procedures were performed on ovarian tissues. Follicle number and diameter of all groups were calculated and evaluated under the light microscope. When primary follicle number and diameters were compared statistically among the groups, there was a remarkably meaningful difference between the EMF group and the control, 20 mg carnosine and EMF+20 mg carnosine groups (p<0.05). There were significant irregularities in the structure of the oocyte and the granulosa cells surrounding the oocyte, especially in the EMF-treated groups. However, the structure of the oocyte and the granulosa cells surrounding the oocyte in the EMF+20 mg carnosine group showed a more regular structure compared to the EMF group. In this study, it can be concluded that the number and diameter of ovarian follicles decreased in rats exposed to electromagnetic field and 20 mg of carnosine may prevent damage caused by EMF.