Yazar "Ovey, Ishak Suat" seçeneğine göre listele

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    Low level laser application reduces the effect of 5 fluorouracil/leucovorin combination on human breast cancer cells
    (2020) Ovey, Ishak Suat; Nemutlu Samur, Dilara; Karabulut, Bayhan
    Aim : 5-fluorouracil has been widely used in breast cancer treatment. Low level laser therapy (LLLT) has been shown to modulate biological processes and used in cancer treatment. It has been reported that apoptosis is induced by stimulation of transient receptor potential protein channels in numerous cancer cells, including breast cancer. We aimed to reveal the effects of 5-fluorouracil / Leucovorin (5-FU/LV) and . low level laser on apoptosis via transient receptor protein ancyrin 1 (TRPA1) channels. Material and Methods: Breast cancer cells (MCF-7) were cultured and cells were divided into seven main groups. Cells were incubated with 5-FU/LV and LLLT exposure separately and together performed on MCF-7 cell cultures. Cell cultures incubated with TRPV1 channel antagonist capsazepin and stimulator capsaicin. The effects of 5-FU and LLLT were investigated on all molecular pathways of apoptosis. Results: It was found that the levels of apoptosis in 5-FU/LV group were significantly increased in cancer cells compared to control group .TRPA1 channel stimulator administration resulted in significantly increased apoptosis levels compared to the control group, in 5-FU/LV + low level laser group the apoptosis level significantly reduced compared to 5-FU/LV-only group. (p<0.001) Conclusion: It has been shown that 5-FU/LV significantly increases apoptosis in breast cancer cells, however low level laser administration decreases apoptosis and suppressed apopitotic effect of 5-FU/LV significantly on breast cancer cells.
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    Selenium enhances the TRPM2 mediated effect of paclitaxel on human breast cancer cells
    (2020) Guler, Yilmaz; Ovey, Ishak Suat
    Aim: Paclitaxel is widely used in adjuvant treatment of early breast cancer and second-line treatment of metastatic breast cancer. It has been reported that transient receptor potential melastatin-2 (TRPM2) channels are expressed intensively in breast cancer and has significant effects on oxidative stress. Selenium is an essential element and has effects on reproduction, toxicity, antiaging and DNA reproduction. In this study, we aimed to reveal the role of selenium and TRPM2 channels on apoptotic effects of paclitaxel in breast cancer cells. Material and Methods: Breast cancer cells (MCF-7) were cultured and cells were divided into seven main groups. Cells were incubated with paclitaxel and selenium separately and together administrated on breast cancer cell cultures. Cell cultures incubated with TRPM2 channel antagonist anthranilic acid and stimulator cumene-hydroperoxyde. The effects of paclitaxel and selenium were invastigated on molecular pathways of apoptosis.Results: It was found that the levels of apoptosis in paclitaxel group were significantly increased in cancer cells compared to control group (p0,001).TRPM2 channel stimulator cumene-hydroperoxyde administration resulted in significantly increased apoptosis levels compared to the control group (p0.001) and it was found that in pacliatxel + selenium group the apopitosis level significantly increased compared to paclitaxel-only group (p0.001).Conclusion: As a result of our study, it has been shown that paclitaxel significantly increases apoptosis in breast cancer cells, and this effect directly related the TRPM2 channels. It was found that the application of selenium in cell culture medium in non-toxic doses increased the TRPM2 mediated apoptotic activity of paclitaxel.