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Öğe Capsaicin Reduces Kidney Injury in a Sepsis Model Induced by Cecal Ligation and Puncture(Wiley, 2025) Bircan, Burak; Firat, Semanur; Cakir, Murat; Sekerci, Guldeniz; Oz, Samet; Aydin, Ali[No abstract available]Öğe Cytotoxic and Genotoxic Effects of Nateglinide on A2780, LNCaP and Caco2 Cell Lines(Wiley, 2023) Oz, Samet; Yuksel, Furkan; Sekerci, Guldeniz; Tekin, Suat[Abstract Not Available]Öğe The Effect of Capsaicin on Oxidative Stress Parameters in Kidney in Sepsis Model Induced by Cecal Ligation and Puncture(Wiley, 2023) Firat, Semanur; Bircan, Burak; Cakir, Murat; Sekerci, Guldeniz; Oz, Samet[Abstract Not Available]Öğe The Effect of TRPA1 Channel Agonist ASP7663 and Antagonist HC-030031 on Oxidative Stress Parameters in Renal Ischemia Reperfusion in Rats(Wiley, 2023) Firat, Semanur; Sekerci, Guldeniz; Cakir, Murat; Bircan, Burak; Oz, Samet[Abstract Not Available]Öğe Inhibition of Transient Receptor Potential Ankyrin 1 Channels Reduces Renal Ischemia Reperfusion Injury(Wiley, 2025) Firat, Semanur; Cakir, Murat; Bircan, Burak; Aydin, Ali; Sekerci, Guldeniz; Oz, Samet[No abstract available]Öğe Investigation of the Effects of Saxagliptin in In Vitro and In Vivo Models of Diabetic Neuropathy(Wiley, 2025) Oz, Samet; Orhan, Seval Ulku; Taslidere, Asli; Ozcan, Mete; Tekin, SuatDiabetic neuropathy (DN), one of the most common microvascular complications of diabetes, is a condition involving complex pathophysiological mechanisms such as oxidative stress, inflammation, apoptosis, primarily resulting from chronic hyperglycemia. This study aimed to evaluate potential effects of Saxagliptin (Sax), a DPP-4 enzyme inhibitor, on in vitro and in vivo models of DN. Dorsal root ganglion neurons isolated from 1 to 2-day-old Wistar Albino rats were exposed to a high-glucose environment for 24 h to induce in vitro DN model. In this model, effects of Sax on cell viability and associated intracellular signaling pathways were investigated. In the in vivo model, streptozotocin-induced diabetic mice were divided into four groups: control, DN, DN+Sax-2, DN+Sax-10 (n = 10). For 15 days, DN group received 0.9% isotonic sodium chloride, while DN+Sax-2 and DN+Sax-10 groups were administered Sax orally via gavage at doses of 2 and 10 mg/kg, respectively, with concurrent nociceptive behavioral testing. At end of experiment, animals were decapitated, biochemical and histological analyses were performed on collected blood and pancreatic tissues. Sax, significantly increased cell viability via phosphoinositide 3-kinase pathway (p < 0.05). Compared to DN group, Sax-treated groups showed improvements in mechanical allodynia and thermal hyperalgesia; increased levels of superoxide dismutase, catalase, glutathione, total antioxidant status, interleukin-10; decreased levels of malondialdehyde, interleukin-1 beta, interleukin-6 (p < 0.05). Additionally, caspase-3 expression in pancreatic tissue was suppressed, and histopathological damage was markedly reduced (p < 0.0001). These findings suggest that Sax suppresses inflammation, inhibits oxidative damage and apoptosis, thereby reducing hyperalgesia, and may have therapeutic effects against DN.Öğe Microwave Synthesis, Evaluation, and Docking Study of Amino Acid Derivatives of 7-Chloroquinoline: Exploring Cytotoxic and Antioxidant Potentials(Amer Chemical Soc, 2026) Ezugwu, James A.; Kucukbay, Fatumetuzzehra; Oz, Samet; Keskin, Tuba; Boulebd, Houssem; Tekin, Suat; Kucukbay, HasanNew carbamate and amino acid derivatives of 7-chloroquinoline were synthesized and characterized using FTIR, 1H NMR, 13C NMR, and HRMS analysis. The synthesized compounds were obtained through a benzotriazole-mediated approach via microwave synthesis and evaluated for antioxidant and anticancer activities. All the synthesized compounds have antioxidant properties though less than those of the standard. Cytotoxic activities of new 7-chloroquinolinyl benzyl amino carbamate derivatives were accessed using LNCaP (Lymph Node Carcinoma of the Prostate), A2780 (human ovarian cancer), and MCF-7 (breast cancer) cell lines. For cytotoxicity research, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used. The synthesized compounds were subjected to a cell viability assay, and following a 24 h treatment, the IC50 values were determined. Among all the tested compounds, compound 4b demonstrated comparable antitumor activity against LNCaP, A2780, and MCF-7 cell lines when compared to the standard drug docetaxel with IC50 values of 6.61, 2.81, and 5.69 mu g/mL for LNCaP, A2780, and MCF-7 cell lines, respectively. A molecular docking study targeting the beta-tubulin enzyme revealed that compounds 4a, 4b, and 5b exhibit a high affinity for the taxane binding site and may mimic the action of docetaxel.Öğe New sulfa drug derivatives and their zinc(II) complexes: synthesis, spectroscopic properties and in vitro cytotoxic activities(Taylor & Francis Inc, 2024) Sahal, Hakan; Oz, Samet; Keskin, Tuba; Tekin, Suat; Canpolat, Erdal; Kaya, MehmetSynthesis of four sulfa drug derivatives (L-1-L-4) and Zn(II) complexes derived from sulfonamide group antibiotic substances was carried out using the hydrothermal technique (HT) and their structures of the obtained compounds were explained using elemental analysis (EA), FT-IR and NMR (H-1- and C-13-). Cytotoxic activities of four novel sulfa drug based-Schiff base compounds and their Zn(II) complexes were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay using MCF-7 (human breast cancer), Caco-2 (human colorectal adenocarcinoma), A2780 (human ovarian cancer) and LNCaP (human prostate adenocarcinoma) cell lines. LogIC50 values of all obtained compounds were computed with the Graphpad Prism 6 program after 24 h of treatment for MCF-7, Caco-2, A2780 and LNCaP cells. Comet assay experiments were performed using LogIC(50) concentrations of all compounds to determine DNA damage. Based on the data obtained, all compounds significantly decreased MCF-7, Caco-2, A2780 and LNCaP cell viability compared to the control groups (p < 0.05). Communicated by Ramaswamy H. SarmaÖğe Phoenixin-14 Administration Acts Protective Role in Rats with Experimental Acute Kidney Injury Model(Wiley, 2025) Oz, Samet; Bahar, Mehmet Refik; Sekerci, Guldeniz; Cetin, Asli; Tekin, Suat[No abstract available]Öğe Protective Effect of Transient Receptor Potential Ankyrin 1 Inhibition on Renal Ischemia Reperfusion Injury in Rats(Wiley, 2025) Cakir, Murat; Aydin, Ali; Firat, Semanur; Sekerci, Guldeniz; Bircan, Burak; Oz, SametThe transient receptor potential ankyrin 1 (TRPA1) channels, characterized as nonselective cation channels with permeability to calcium ions (Ca2 +), are part of the extensive family of transient receptor potential (TRP) channels. Research has demonstrated that TRPA1 channels function as sensors for oxidative stress in the renal tubules. Additionally, TRPA1 expression has increased in renal tissue following ischemia-reperfusion (IR). There is also a significant correlation between IR-induced renal injury and TRPA1 expression. This study investigated the effects of selective TRPA1 agonist ASP7663 and selective TRPA1 antagonist HC-030031 on renal IR injury. A total of 40 rats were divided into four groups: control, IR, IR+ASP7663, and IR + HC-030031. The rat kidneys were exposed to 45 min of ischemia, followed by 24 h of reperfusion. TRPA1 agonist ASP7663 and selective TRPA1 antagonist HC-030031 were administered intraperitoneally to the treatment groups with renal IR. HC-030031 administration reduced the elevated kidney injury molecule-1 (KIM-1), blood urea nitrogen (BUN), and creatinine (Cre) caused by renal IR. HC-030031 administration reduced the increased histopathological damage in renal tissue due to IR. It also reduced renal tissue interleukin-1beta (IL-1 beta), interleukin-6 (IL-6), toll-like receptor-4 (TLR-4), phosphorylated-NF-kappa B, phosphorylated-I kappa B-alpha, tumor necrosis factor-alpha (TNF-alpha), and caspase-3 levels. In this study, TRPA1 antagonist HC-030031 showed a protective behavior on renal IR injury by averting inflammation and apoptosis. After further studies, TRPA1 inhibition may be a new treatment strategy to prevent renal IR injury.Öğe Protective Effects of Phoenixin-14 Administration Against Renal Ischemia/Reperfusion Injury in Rats(Wiley, 2025) Oz, Samet; Bahar, Mehmet Refik; Sekerci, Guldeniz; Taslidere, Asli; Tekin, SuatPhoenixin (PNX), identified in the rat hypothalamus in 2013, has two bioactive isoforms with 14 and 20 amino acids. Initially studied for its role in reproductive regulation, research has since shown that PNX also prevents visceral pain, enhances memory, and aids heart tissue recovery. However, its role in kidney tissue remains unclear. Due to its antioxidant properties, PNX may help reduce oxidative stress and cellular damage in organs. This study was designed to determine the potential protective effects of Phoenixin-14 (PNX-14) against renal ischemia/reperfusion (I/R)-induced injury in rats. 40 male Wistar Albino rats were divided into four groups: Control, I/R, PNX-14 (50 mu g/kg), and PNX-14 (100 mu g/kg) (n = 10). All groups except the control group underwent 45 min of bilateral ischemia followed by 24 h of reperfusion. PNX-14 (50 and 100 mu g/kg, intraperitoneally) was administered 1 h before induction of ischemia. Both doses of PNX-14 reduced the levels of acute kidney injury markers (blood urea nitrogen and creatinine) in blood tissue (p < 0.05). PNX-14 increased the activity of antioxidant enzymes (superoxide dismutase and catalase) and the levels of glutathione, while reducing malondialdehyde (p < 0.05). Histological evaluation of the I/R group revealed significant histopathological findings, and it was found that PNX-14 administration improved these histological damages (p < 0.05). These results suggest that PNX-14 provides protection against renal injury induced by I/R. After further studies, PNX-14 may be a new therapeutic strategy to prevent renal I/R injury.Öğe Synthesis, structural characterizations and in vitro cytotoxic activities of new sulfonamide-based Schiff base derivatives(Taylor & Francis Inc, 2023) Sahal, Hakan; Oz, Samet; Tekin, Suat; Canpolat, ErdalSynthesis of five different new compounds (1-5) were carried out. Their structures were characterized by spectroscopic methods such as Fourier transform infrared, Proton nuclear magnetic resonance, Carbon-13 nuclear magnetic resonance and Elemental analysis. Cytotoxic activities of five new sulfonamide based-Schiff base compounds were determined by MTT assay using A-2780 (human ovarian cancer) and MCF-7 (breast cancer) cell lines. LogIC(50) values of the sulfonamide derivates compounds were calculated by Graphpad Prism 12 programme after a 24-hour treatment for A2780 and MCF-7 cells. Comet assay experiments were performed to determine DNA damage using LogIC(50) concentrations of all compounds in A2780 and MCF-7 cells. All compounds significantly reduced A2780 and MCF-7 cell viability compared to control groups (p < 0.05). In addition, all compounds caused DNA damage in A2780 and MCF-7 cells (p < 0.05). These results show that the synthesized compounds exhibit cytotoxic effects against cancer cells and that the cause of cell death is due to DNA damage. Communicated by Ramaswamy H. Sarma
