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    Comparison of indigo carmine decolorization by Pseudomonas aeruginosa and crude laccase enzyme from Funalia trogii
    (Tubitak Scientific & Technological Research Council Turkey, 2019) Boran, Filiz; Birhanli, Emre; Yesilada, Ozier; Ozbey, Elif
    The effects of incubation time, temperature, initial pH, and dye concentration on the indigo carmine decolorization activity of Pseudomonas aeruginosa ATCC 10145 and some factors on the decolorization potential of crude laccase enzyme obtained from Funalia trogii ATCC 200800 were comparatively investigated. This bacterium showed effective decolorization activity at all agitation and temperature values. Indigo carmine was greatly decolorized by P. aeruginosa at all pH values except pH 10. A decrease in decolorization activity occurred with increasing dye concentration, but this bacterium effectively decolorized the dye within 24 h. The decolorization process was through microbial metabolism, not biosorption. No decolorization or laccase activity could be obtained by the cell-free intracellular extract or culture filtrate of this bacterium. On the other hand, crude laccase effectively decolorized indigo carmine under highly acidic conditions, especially at pH 3.0 as 57% in 300 seconds. This activity decreased progressively due to the increase in pH values. In a short incubation period and at high temperature values, the crude laccase enzyme removed the color of the dye at 50 degrees C (56%), 60 degrees C (45%), and 70 degrees C (38%). These data are important for improving methods for decolorization of textile dyes used at high temperatures in various industrial applications.
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    THE EFFECT OF RADIATION ON ANTIOXIDANT SYSTEM OF ESCHERICHIA COLI AND RADIATION RESISTANT DEINOCOCCUS RADIODURANS
    (Parlar Scientific Publications (P S P), 2019) Ozbey, Elif; Asma, Dilek
    In last 30-35 years, it has been discovered that many organisms can live in environments considered as extreme for mankind. Since these microorganisms can live in the extreme environments, they are named as extremophiles. One of the discovered microorganisms, Deinococcus radiodurans, had drawn attention for its extraordinary capabilities and its potential of infinity. D. radiodurans is resistant to genotoxic chemicals, oxidative damage, high-level ionization, ultraviolet radiation and dehydration. Because of these features one can name it polyextremophilic. In recent years, studies on bioremediation and related to heavy metal and detoxification of xenobiotics which cause serious environment problems, as well as genetic engineering, have been intense. However, the reactive oxygen species, induced by UV and their effects, their cellular defense mechanisms against ROS and about antioxidative systems responsible for cleaning of reactive oxygen species enough research has not been made.To this end; in Ecsherichia coli and radiation-resistant D. radiodurans bacteria were compared and tested effects on levels reduced glutathione (GSH) and antioxidant system of radiation. In both bacteria, enzyme activity and GSH levels has been determined after the different doses of gamma radiation applications. In the end of our study, high activities in enzyme especially superoxide dismutase and catalase of D. radiodurans were observed depending on the different doses gamma radiation applications. We were also observed that GSH quantity and other enzymes had a low activity in the cell. Moreover, it was determined that enzyme and GSH levels were decreased in E. coli depending on the radiation levels.
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    In UV-C, Deinonoccus radiodurans and Vitreoscilla Hemoglobin (vgb) Gene Transferred Recombinants; Effect on SOD, KAT and Carotene Amount
    (Kahramanmaras Sutcu Imam Univ Rektorlugu, 2022) Ozbey, Elif; Asma, Dilek
    Ultraviolet radiation (UV); creates oxidative stress by causing the formation of reactive oxygen species in biological tissues. Reactive oxygen species induced by UV. their effects and cellular defense mechanisms against them, and antioxidant systems responsible for cleaning reactive oxygen species are the subjects of much research today. In this study, Deinococcus radiodrans which is well known an extremophile for its resistance to many agents and conditions such as high levels of ionizing radiation and UV radiation, drought and chemicals that damage DNA and Vitreoscilla hemoglobin (vgb) gene cloned recombinant with. and vgb recombinant strain as a control were used. The effect of UV-C on the antioxidant defense systems of D. radiodurans (superoxide dismutase, catalase and carotene) was investigated, and in addition, the contribution of the vgb(-) gene, which provides more oxygenated environment to the organism, to the UV resistance of the bacteria, was investigated. Accordingly, when UV-C treated samples of D. radiodurans (vgb(-)) were compared with the control groups, it was determined that the superoxide dismutase and catalase enzyme activities were lower than the wild and the recombinant. carrying the vgb gene. Again, in wild-type bacteria with high carotene. an increase in the amount of carotene was clearly observed due to UV-C application.