DSpace Arşivi :: by Yazar "Ozgur, Mustafa Erkan" değerine göre listeleniyor

Yazar "Ozgur, Mustafa Erkan" seçeneğine göre listele

Listeleniyor 1 - 14 / 14

Characterization of the Embryonic and Larval Development of Wild Pike-Barb (Luciobarbus esocinus) Biological Parameters, and the Possibility of Breeding
(Israeli Journal Of Aquaculture-Bamidgeh, 2016) Ozgur, Mustafa Erkan; Akbulut, Aydin; Demirsoy, Ali
This study was conducted to obtain preliminary data on the possibility of breeding this species through artificial insemination and to determine the incubation period at different temperatures (degrees C) of hatchery water [20 (A1), 22 (A2), 25 (A3) and 26 (A4); (+/- 1)] and to determine the morphological and larval growth parameters of L. esocinus. The blastoderm formed an embryonic shield 43 h (A1 and A2) and 36 h (A3 and A4) after insemination. The eggs hatched after 113 h (A1 and A2) and 61.5 h (A3 and A4) after insemination, and new larvae were observed. Exposing the newly fertilized eggs to various temperatures during the incubation period resulted in significantly longer duration of embryo formation, egg hatching and yolk sac consumption (h) at 20 degrees C and 22 degrees C than at 25 degrees C and 26 degrees C (P < 0.05). No significant differences were observed between eggs exposed to 20 degrees C and 22 degrees C and those exposed to 25 degrees C and 26 degrees C (P > 0.05). However, significant differences in day-degree of hatching were observed among all the groups (P < 0.05).
The Cytotoxicity, DNA Fragmentation, and Decreasing Velocity Induced By Chromium(III) Oxide on Rainbow Trout Spermatozoa
(Springernature, 2023) Ozgur, Mustafa Erkan; Ulu, Ahmet; Gurses, Canbolat; Ozcan, Imren; Noma, Samir Abbas Ali; Koytepe, Suleyman; Ates, Burhan
The present study aimed to determine the cytotoxicity of chromium(III) oxide micro particles (Cr2O3-Ps) in rainbow trout (Oncorhynchus mykiss) spermatozoa. Firstly, Cr2O3-Ps were synthesized and structurally characterized the surface, morphological for particle size and thermal properties. In addition, its structural and elemental purity was determined using energy-dispersive X-ray (EDX) spectrum and elemental maps. Structural purity, thermal properties, and stability of Cr2O3 -Ps were also examined in detail by performing thermal analysis techniques. The cytotoxicity of Cr2O3-Ps was measured by the observation of velocities, antioxidant activities, and DNA damages in rainbow trout spermatozoa after exposure during 3 h in vitro incubation. The straight line velocity (VSL), the curvilinear velocity (VCL), and the angular path velocity (VAP) of spermatozoa decreased after exposure to Cr2O3-Ps. While the superoxide dismutase (SOD) and the catalase (CAT) decreased, the lipid peroxidation increased in a dose-dependent manner. However, the total glutathione (tGSH) was not affected in this period. DNA damages were also determined in spermatozoa using Comet assay. According to DNA in tail (%) data, DNA damages have been detected with gradually increasing concentrations of Cr2O3-Ps. Furthermore, all of class types which are categorized as the intensity of DNA fragmentation has been observed between 50 and 500 mu g/L concentrations of Cr2O3-Ps exposed to rainbow trout spermatozoa. At the end of this study, we determined that the effective concentrations (EC50) were 76.67 mu g/L for VSL and 87.77 mu g/L for VCL. Finally, these results about Cr2O3-Ps may say to be major risk concentrations over 70 mu g/L for fish reproduction in aquatic environments.
Detection and Counting of Oncorhynchus Mykiss Spermatozoa
(Ieee, 2015) Okumus, Fatih; Kocamaz, Adnan Fatih; Ozgur, Mustafa Erkan
Today in the world, a large portion of the trout species grown in the fisheries constitutes Oncorhynchus Mykiss. In order to increase the efficiency of Oncorhynchus Mykiss's production, it is expected to be in fair value of its sperm quality parameters for fertilize. Investigation by examination under a microscope with the conventional method of quality parameters is the waste of time for researchers. In this work, we present a system that can detect the cell concentration, which is an important quality parameter for the fish spermatozoa, with Otsu Threshold and Connected Component Labeling methods.
Evaluation of Relationship between Sperm Cell Velocities and Fatty Acids Contents of Semen Seminal Fluid in the Two Trout Fish Species
(Zoological Soc Pakistan, 2023) Ozgur, Mustafa Erkan; Erdogan, Selim; Aydemir, Songul; Yumusakbas, Hatice
In this study, the fatty acid compositions of semen seminal plasma in rainbow trout (Oncorhynchus mykiss) and brown trout (Salmo trutta fario) were analyzed in fresh semen by gas chromatography (GC) and investigated the relationships with the sperm cell velocities. According to our results, total levels of saturated fatty acids (SFA) and monounsaturated fatty acids (MUFA) for Salmo trutta fario occurred in higher quantities than Oncorhynchus mykiss but the quantities of total polyunsaturated fatty acids (PUFA) were less. Palmitic and stearic acid for SFA, oleic and nervonic acids for MUFA and linolenic, arachidonic, eicosapentaenoic and docosahexaenoic acids for PUFA of semen seminal plasma were main. Semen volume and the levels of SFA (myristic, palmitic and margaric acid), MUFA (palmitoleic, ginkgolic, oleic and nervonic acid) and PUFA (linoleic, gama-linolenic and eicosadienoic acid) were significantly different (p<0.05) between both species. Especially, the levels of eicosapentaenoic (EPA) and docosahexaenoic (DHA) determined no significant different (p>0.05). Additionally, we found negative correlation between the straight line velocity (VSL) and pentadecanoic acid (r =-0.78, p<0.05), stearic acid (r =-0.76, p<0.05) and nervonic acid (r =-0.89, p<0.01) for rainbow trout, while there was negative correlation (r =-0.96, p<0.05) between angular path velocity (VAP) and EPA for brown trout. Finally, it can be concluded with these results that the fatty acids of sperm seminal plasma affect the velocities of sperm cells and they would also improve semen quality and fertilization protocols.
THE IN VITRO EFFECTS OF SILICA NANOPARTICLES ON SPERMATOZOON OF RAINBOW TROUT (ONCORHYNCHUS MYKISS)
(Parlar Scientific Publications (P S P), 2018) Ozgur, Mustafa Erkan
In recent years, silicon dioxide (SiO2) nanoparticles (NPs) have widely used in industry, personal care products, cosmetics, construction, catalysis, dyes (pigments) and pharmaceutical applications. This use of nano-SiO2 has been associated with environmental toxicity concerns. Therefore, the aim of this study was to evaluate the effect of SiO2 NPs on the motility parameters of Oncorhynchus mykiss spermatozoon. Afterwards, Sperm samples were incubated with different concentrations of SiO(2)NPs (1, 10, 25, 50 and 100 mg/L) at 4 degrees C for 3 h. According to the obtained results, there were statistically significant (p < 0.05) differences in VSL (m/s), VAP (mu m/s), BCF (Hz) and MAD (degrees) values, while there was no significant difference (p > 0.05) in VCL (mu m/s), LIN (%) and ALH (mu m) values in spermatozoon motility against SiO2 NPs exposures at different concentrations. As a result, especially 100 mg/L concentration of SiO2 NPs has negative effects on rainbow trout (Oncorhynchus mykiss) spermatozoon in terms of sperm motility.
The in vitro toxicity analysis of titanium dioxide (TiO2) nanoparticles on kinematics and biochemical quality of rainbow trout sperm cells
(Elsevier Science Bv, 2018) Ozgur, Mustafa Erkan; Balcioglu, Sevgi; Ulu, Ahmet; Ozcan, Imren; Okumus, Fatih; Koytepe, Suleyman; Ates, Burhan
In recent years, titanium dioxide (TiO2) nanoparticles (NPs) as metal oxide nanoparticles are widely used in industry, agriculture, personal care products, cosmetics, sun protection and toothpaste, electronics, foodstuffs and food packaging. This use of nano-TiO2 has been associated with environmental toxicity concerns. Therefore, the aim of this study was to evaluate the in vitro effect of different doses of TiO2 NPs (similar to 30-40 nm) (0.01, 0.1, 0.5, 1, 10 and 50 mg/L) at 4oC for 3 h on the sperm cell kinematics as velocities of Rainbow trout (Oncorhynchus mykiss, Walbaum, 1792) sperm cells. Furthermore, oxidative stress markers (total glutathione (TGSH) and superoxide dismutase (SOD) were assessed in sperm cells after exposure to TiO2 NPs. According to the obtained results, there were statistically significant (P < 0.05) decreasing in the velocities of sperm cells after 10 mg/L TiO2 NPs and an increase the activity of SOD (P < 0.05) and TGSH levels were determined.
An investigation of histopathological changes and bioaccumulation in tissues of rainbow trout (Oncorhynchus mykiss) after exposure to dodine
(Taylor & Francis Ltd, 2022) Buyuksoylu, Semih; Ozgur, Mustafa Erkan; Gul, Cemile Ceren; Taslidere, Asli; Aydemir, Songul; Erdogan, Selim
The purpose of this research is to determine ecotoxicological effects of dodine (n-dodecylguanidini acetate) on aquatic environments. Though dodine is widely used as a fungicide in agriculture, but there is no much data about its ecotoxicology. In this regard, we investigated bioaccumulation levels and histological alterations on the tissues of muscle, liver and gills in Rainbow Trout (Oncorhynchus mykiss) against different doses (0.01, 0.1, 0.5 and 1mg/L) of Dodine exposure. The tissues of fish were extracted according to QUECHERS method and analyzed by mass spectrometer (LC-MS-MS). Neither of the applied dodine doses resulted in killing 50% of the total individuals in the experimental groups. However, 48hours after doses, behaviors such as instability, anomaly in swimming or sudden jumping movements were observed. Histological results of the study showed deteriorations of the radiological pattern of hepatocytes, sinusoidal dilatations, hemorrhages, edemas, mononuclear cell infiltrations, vascular congestions, hyperplasia and hypertrophy in liver, gill and muscle tissues. Accumulation of dodine in tissues correlated with increase of dose. The maximum level of active substance accumulation in tissues were measured 96hours after application of 1mg/L dodine dose -in order- in gills, muscles and liver. The accumulations were statistically significant (p<0.05) when compared with control group.
Investigation of toxic effects of amorphous SiO2 nanoparticles on motility and oxidative stress markers in rainbow trout sperm cells
(Springer Heidelberg, 2019) Ozgur, Mustafa Erkan; Ulu, Ahmet; Ozcan, Imren; Balcioglu, Sevgi; Ates, Burhan; Koytepe, Suleyman
In this study, we investigated the effects of SiO2 nanoparticles (SiO2-NPs) (1, 10, 25, 50, and 100mg/L) for 24h in vitro on the motility parameters and oxidative stress markers such as total glutathione (TGSH), catalase (CAT), and malondialdehyde (MDA) of rainbow trout, Oncorhynchus mykiss sperm cells. Therefore, SiO2-NPs were synthesized with sol-gel reaction from tetraethoxy orthosilicate (TEOS). The prepared nanoparticle structures were characterized for chemical structure, morphology and thermal behavior employing Fourier transform infrared spectroscopy, X-ray spectroscopy, scanning electron micrograph, and thermal analysis (DTA/TGA/DSC) techniques. After exposure, there was statistically significant (p<0.05) decreases in velocities of sperm cells. CAT activity significantly (p<0.05) decreased by 9.6% in sperm cell treated with 100mg/L. In addition, MDA level significantly increased by 70.4% and 77.5% in sperm cell treated with 50 and 100mg/L SiO2-NPs, respectively (p<0.05). These results showed that SiO2-NPs may have toxic effect on rainbow trout sperm cells in 50mg/L and more.
Melatonin protects sperm cells of Capoeta trutta from toxicity of titanium dioxide nanoparticles
(Springer Heidelberg, 2020) Ozgur, Mustafa Erkan; Ulu, Ahmet; Noma, Samir Abbas Ali; Ozcan, Imren; Balcioglu, Sevgi; Ates, Burhan; Koytepe, Suleyman
In this study, it was aimed to determine the protective effects of melatonin (0.01, 0.1, and 1 mM) against 10 mg/L titanium dioxide nanoparticles (TiO2-NPs) on kinematic and oxidative indices in the sperm cells of Capoeta trutta. Therefore, TiO2 nanoparticles were synthesized primarily within the scope of the study. The synthesized nanoparticles were characterized by structurally different techniques. Then, melatonin and TiO2 were applied to Capoeta trutta sperm cells by in vitro. According to our data, all doses of melatonin showed protective effects on all velocities of sperm cells such as the straight line velocity (VSL), the curvilinear velocity (VCL), and the angular path velocity (VAP) against TiO2-NPs, while 0.1 and 1 mM doses of melatonin improved the VSL value. Although TiO2-NPs increased total glutathione (tGSH), malondialdehyde (MDA) lipid peroxidation, and superoxide dismutase (SOD) compared to the control group, there were positive treatment effects for all doses of melatonin on antioxidant capacity of sperm cells. At the end of this research, it is suggested that over 0.1 mM dose of melatonin improves the velocity of sperm cells and it plays a protective role against the toxic effects of TiO2-NPs.
Mid-autumn spermiation in outdoor-cultured pikeperch (Sander lucioperca) using different gonadoliberin application strategies
(Elsevier, 2021) Ozgur, Mustafa Erkan; Erdogan, Selim; Raskovic, Bozidar; Fazekas, Georgina; Ljubobratovic, Uros
This study aimed to find the optimal strategy for the application of salmon gonadotropin-releasing hormone analogue (sGnRHa) in outdoor-cultured pikeperch males in an exceptionally early term in early November. According to the histological examination, on the day of hormonal treatment, pikeperch testes were in the late stadium of spermatogenesis, with 78.7% of germ cells attributed to sperm cells. Five experimental groups were established varying on hormonal preparations and water temperature: (1) sGnRHa (25 mu g kg(-1)) was applied to fish at pre-warming at water temperature of 11 degrees C (GnRH-LOW); (2) sGnRHa (25 mu g kg(-1)) was applied to fish post-warming at water temperature of 15 degrees C (GnRH-HIGH); (3) priming group (5 mu g kg(-1) at 11 degrees C +25 mu g kg(-1) at 15 degrees C); (4) reference group - fish were injected with human chorionic gonadotropin; (5) control group -fish were injected with saline solution. Sperm was obtained from all fish in hormonally treated groups, whereas the fish treated with saline did not spermiate. The greatest values of the straight-line velocity (VSL), the curvilinear velocity (VCL), and the angular path velocity (VAP) of sperm cells, as well as the amounts of Mg2+, K+, and Na+ in the sperm seminal plasma, were obtained in GnRH-HIGH, whereas the GnRH-LOW group yielded the greatest sperm volume. Among the ions in seminal plasma, K+ and Na+ dominated and showed a relationship with sperm kinematic parameters. According to the obtained data, sGnRHa can be considered as an appropriate hormonal preparation to induce spermiation at an early preseason. Either before or after warming, sGnRHa application can yield sperm of proper quantity and quality as early as 5 months before the natural spawning season. A comprehensive study is recommended to evaluate the full period of sperm availability in outdoor-reared males, followed by direct evaluation of the fertilizing capacity.
Pharmacokinetic Profiles of Metamizole Metabolites after Intramuscular and Intravenous Administration in Healthy Arabian Horses
(Wiley, 2021) Yilmaz, Ismet; Maras, Zeynep; Ozgur, Mustafa Erkan; Durmaz, Murat; Ulusoy, Halil Ibrahim; Erdogan, Selim; Ugur, Yilmaz
Metamizole sodium (MT) is an analgesic and antipyretic drug molecule used in humans, horses, cattle, swine, and dogs. Metamizole rapidly hydrolyzes and turns into methylamino antipyrine (MAA), an active primary metabolite of MT. The present study aims to determine the pharmacokinetic (PK) profiles of MT metabolites after intravenous (IV) and intramuscular (IM) administration into sex of Arabian horses (Equus ferus caballus) using a cross-over study design. The plasma samples were extracted by solid-phase extraction (SPE) method, and plasma concentrations of MT metabolites were analyzed by high-performance liquid chromatography (HPLC). After administrations of MT, plasma concentrations of methylamino antipyrine (MAA), amino antipyrone (AA), and acetylamino antipyrone (AAA) were determined within range of 15 min-12 h. Plasma concentrations of AA and AAA were lower than the plasma concentrations of major metabolite MAA at each sampling point. The PK parameters were statistically evaluated for MT's metabolites between male and female horses and also between IM and IV administrations of PK parameters such as C-max, t(max), t(1/2 lambda z), AUC(0-t), AUC(0-infinity), lambda z, Cl and V-ss (p < .05). The AUC(IM)/AUC(IV) ratio in female and male horses for MAA was 1.19 and 1.13, respectively. The AUC(IM)/AUC(IV) ratio for AA was lower than those found for MAA. AUC(IM)/AUC(IV) ratio was statistically significantly different between male and female horses for AA (p < .05). According to these results, some PK parameters such as Cmax, AUC, and MRT, MAA and AA concentrations have shown statistically significant differences by MT administrations.
The relationship between semen seminal plasma ions and sperm cell velocnies of wild-caught longspine scraper, Capoeta trutta
(Copernicus Gesellschaft Mbh, 2019) Ozgur, Mustafa Erkan; Maras, Zeynep; Erdogan, Selim
In this study, semen seminal plasma contents and the motility of sperm cells were determined in Capoeta trutta via a computer-assisted sperm analysis system. In addition, we evaluated the relationship between semen seminal plasma ions and the velocities of sperm cells. Although the predominant ions were K (206.84 +/- 20.61 mg L-1) and Na (128.06 +/- 23.82 mg L-1) in the semen seminal plasma, Ca (14.05 +/- 4.13 mg L-1) and Mg (3.35 +/- 0.44 mg L-1) were not predominate according to our results. However, partially strong relationships between the curvilinear velocity value (VCL) and K (R-2 = 0.67; p <0.05) were found, while it was moderate with Mg (R-2 = 0.48; p <0.05). There was a weak relationship with Na (R-2 = 0.17; p <0.05) and Ca (R-2 = 0.34; p <0.05). In our results, while the trace metals were determined as Zn > Al > B > Li > Cu in semen seminal plasma, they are not correlated with sperm cell velocities. Finally, we hope that the present information on the motility parameters of Capoeta trutta in this paper will eventually help artificial insemination in reproduction practices.
SOME QUALITY PARAMETERS OF SURFACE WATER OF KARASU RIVER IN UPPER PART OF EUPHRATES BASIN, TURKEY
(Carpathian Assoc Environment And Earth Sciences, 2012) Ozgur, Mustafa Erkan; Akbulut, Aydin; Demirsoy, Ali
Research in Euphrates basin in Turkey showed the following values: temperature, dissolved oxygen, pH, nitrite, nitrate, chlorine, sulfate, iron, copper, calcium, magnesium and silica in surface waters were monitored. Significant relationships among these parameters were observed at seasonal term. Some significant (p<0.05) differences were determined among the parameters (temperature, dissolved oxygen demand, nitrite, chlorine, sulfate, iron and copper) according to seasons. pH was not determined a significant (p>0.05) differences in all seasons. However, it was also significant (p<0.05) differences the results of nitrate at summer, calcium and magnesium at spring, silica at winter as other seasons. The data of chlorine, iron, and copper in Karasu River were measured high levels according to water quality standards of EPA.
The Toxicity Assessment of Iron Oxide (Fe3O4) Nanoparticles on Physical and Biochemical Quality of Rainbow Trout Spermatozoon
(Mdpi, 2018) Ozgur, Mustafa Erkan; Ulu, Ahmet; Balcioglu, Sevgi; Ozcan, Imren; Koytepe, Suleyman; Ates, Burhan
The aim of this study was to evaluate the in vitro effect of different doses (50, 100, 200, 400, and 800 mg/L) of Fe3O4 nanoparticles (NPs) at 4 degrees C for 24 h on the kinematics of rainbow trout (Oncorhynchus mykiss, Walbaum, 1792) spermatozoon. Firstly, Fe3O4 NPs were prepared at about 30 nm from Iron (III) chloride, Iron (II) chloride, and NH3 via a co-precipitation synthesis technique. Then, the prepared Fe3O4 NPs were characterized by different instrumental techniques for their chemical structure, purity, morphology, surface properties, and thermal behavior. The size, microstructure, and morphology of the prepared Fe3O4 NPs were studied by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) spectroscopy, and scanning electron microscopy (SEM) equipped with an energy-dispersive X-ray spectrometer (EDS). The thermal properties of the Fe3O4 NPs were determined with thermogravimetric analysis (TGA), differential thermal analysis (DTA), and differential scanning calorimeter (DSC) analysis techniques. According to our results, there were statistically significant (p < 0.05) decreases in the velocities of spermatozoon after treatment with 400 mg/L Fe3O4 NPs. The superoxide dismutase (SOD) and catalase (CAT) activities were significant (p < 0.05) decrease after 100 mg/L in after exposure to Fe3O4 NPs in 24 h. As the doses of Fe3O4 NPs increases, the level of malondialdehyde (MDA) and total glutathione (tGSH) significantly (p < 0.05) increased at doses of 400 and 800 mg/L.

Yazar "Ozgur, Mustafa Erkan" seçeneğine göre listele

Sayfa Başına Sonuç

Sıralama seçenekleri