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Öğe Analysis of gibberellic acid, abscisic acid, indole-3-acetic acid and zeatin from the selected tissues of albino mice(Gordon & Breach Science Publ Inc, Newark, 1997) Yavas A.; Ozmen M.; Topcuoglu S.F.This paper reports the presence of gibberellic acid (GA3), abscisic acid (ABA), indole-3-acetic acid (IAA) and zeatin in the brain, liver and kidney of laboratory mice. The identification of these plant growth regulators (PGRs) in animal tissues after the dietary treatment by GA3 or ABA on F1 offsprings in two different exposure periods was studied. Thin layer chromatographic (TLC) techniques and spectrophotometric assay methods were used for this study. Results show that assayed PGRs accumulate in animal tissues and accumulation of these substances is being attributed to their feed source and exposure to PGR as an effective factor.Öğe Assessment of embryotoxic effects of cadmium, lead and copper on Xenopus laevis(2010) Güngördü A.; Birhanli A.; Ozmen M.This work evaluated the effects of selected metals on embryos using Frog Embryo Teratogenesis Assay- Xenopus (FETAX). Besides FETAX parameters, changes in several biomarkers were studied as early signs of intoxication on Xenopus laevis tadpoles. The acute effects of metals were investigated by using the activity of the enzymes glutathione S-transferase (GST), glutathione reductase, acetylcholinesterase (AChE), carboxylesterase, lactate dehydrogenase, aspartate aminotranferase, and alanine aminotranferase. In the FETAX test, 96-h LC50s were determined as 1.6 mg/L, 96.1 mg/L, and 1.37 mg/L for Cd, Pb, and Cu, respectively. Also, teratogenic index (TI) of each metal was estimated as 1.1, 1.22, and 0.52 for Cd, Pb, and Cu, respectively. The TI showed that tested metals were not teratogenic in X. laevis embryos according to ASTM values. The comparison of selected metals on selected enzyme activities was expected to give useful indications for the proper use of biochemical responses as biomarkers. © by PSP.Öğe Effects of methyl parathion and copper oxychloride application on earthworms (Lumbricus Terrestris) in an apricot orchard(2013) Yologlu E.; Ozmen M.In this study, the effects of methyl parathion and copper oxychloride on earthworms were determined using Lumbricus terrestris due to routine pesticide application in an apricot orchard. The study area was divided into three regions. 250 g Folidol M500 (containing methyl parathion) and 1000 g Oxi-cup 50WG (containing copper oxychloride) were separately dissolved in 250 L of water. These solutions were sprayed over apricot trees in the application areas. In these areas, earthworm samples were collected before (control) and at selected days after pesticide application. The collected earthworm samples were used to determine ethoxyresorufin-O-deethylase (EROD), glutathion-S-transferase (GST), carboxylesterase (CaE), and acetylcholinesterase (AChE) activities. The pesticides used in this study adversely affected non-target organisms in the ecosystem. Due to these adverse effects, some enzyme activities were changed in all application areas on the 1st day after applications. It was also decided that the selected biomarkers, EROD, AChE and CaE, are the suitable enzymes to determine the effects of this kind of pesticide exposure.