Yazar "Porgali, Esra" seçeneğine göre listele
Listeleniyor 1 - 4 / 4
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Acute and chronic effects of electroconvulsive treatment on oxidative parameters in schizophrenia patients(Pergamon-Elsevier Science Ltd, 2011) Kartalci, Sukru; Karabulut, Aysun Bay; Ozcan, Abdul Cemal; Porgali, Esra; Unal, SuheylaElectroconvulsive therapy (ECT) is an effective treatment alternative for schizophrenia. Previous studies have already indicated the possible effects of oxidative stress in this disorder. However, there have been no previous studies evaluating the effects of ECT on the oxidative stress in these patients. We therefore aimed to investigate the acute and chronic effects of ECT on serum levels of oxidant and antioxidant molecules in schizophrenia patients (n = 28). The serum MDA and CAT levels of the patients with schizophrenia were higher than that of the controls before ECT (n = 20) but there was no significant difference in the serum NO and GSH levels of the patient groups compared to the controls. We found that the NO levels of the patients were higher than the controls in the group experiencing their first episode but not in the chronic group. There was a significant clinical improvement in the patients in terms of BPRS. SANS and SAPS reduction after the 9th ECT, but not the 1st ECT. Serum MDA levels were significantly reduced compared to the baseline after the 9th ECT session although there was no significant difference after the 1st session. Separate evaluation of the patient groups revealed that the significant MDA decrease following ECT was in the patients experiencing their first episode and not in the chronic group. No significant difference was noted in the serum levels of other oxidant and antioxidant molecules after either the 1st or 9th ECT session. These results suggest that ECT does not produce any negative effect on oxidative stress in patients with schizophrenia. (C) 2011 Elsevier Inc. All rights reserved.Öğe Catechin-Molecularly Imprinted Cryogel for Determination of Catechin in Red Wines by HPLC-DAD-Fluorescence Detector(Akademiai Kiado Zrt, 2018) Buyuktuncel, Ebru; Porgali, Esra; Ozkara, SerpilA new molecularly imprinted polymer (MIP) was prepared by using catechin (C) as the template molecule. The polymer was characterized by swelling tests, scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET), and Fourier transform infrared (FTIR) spectroscopy. The MIP with high recovery was selected as a solid-phase extraction (SPE) sorbent in this work. The standard solutions were directly applied onto the SPE cartridges following loading, washing, and elution procedures. A solution of the collected fractions was analyzed by high-performance liquid chromatography-diode-array detection (DAD) and fluorescence detector. The optimization of the method and validation was achieved on a C18 column (5 mu m, 250 x 4.6 mm) with methanol-water (35:65, v/v) mixture adjusted pH 2.5 as the mobile phase at a flow rate of 1 mL min(-1) at room temperature. The selectivity coefficient (k) of imprinted p(HEMA-MAH) cryogel was 5.1-fold that of non-imprinted cryogel. It showed good selectivity and affinity for C molecule. A comparison was made between the results obtained with the MIP cartridges and a traditional C18 reversed-phase cartridge. It was observed that 2.3 times higher recovery of C can be obtained on catechin-MIP cryogel. The results of the presented work showed that the prepared MIP can be used as SPE sorbent for extracting of C from red wines.Öğe Determination of phenolic composition and antioxidant capacity of native red wines by high performance liquid chromatography and spectrophotometric methods(Elsevier Science Bv, 2012) Porgali, Esra; Buyuktuncel, EbruA reversed-phase high performance chromatographic method for simultaneous determination of 14 phenolic compounds in native red wines was developed in this study. The identified compounds contained gallic acid, (+)-catechin, 3,4-dihydroxybenzoic acid, chlorogenic acid, (-)-epicatechin, 4-hydroxybenzoic acid, syringic acid, caffeic acid, p-coumaric acid, rutin, resveratrol, myricetin, quercetin and kaempferol. The method includes liquid-liquid extraction of acidic pH with ethylacetate. The analysis used a Zorbax Eclipse XDB-C18 column (5 pm, 4.6 mm x 250 mm). The chromatographic separation of these compounds performed in a single run by using the mobile phase gradient elution of methanol water mixture (% 0.2 formic acid) at room temperature, with flow rate at 1 mL/min. Detection was carried out by UV-vis and fluorescence detector. Each analysis required an equilibration period of 10 min and a run time of 14 min for completion. The optimized chromatographic method was carefully validated for precision and accuracy. Our findings indicated that the developed HPLC method was precise, accurate, specific and sensitive for simultaneous determination of phenolic compounds. Consequently, the described method was applied to the analysis of six wines from Malatya and Elazig. Gallic acid was dominant phenolic acid in red wines. (+)-catechin, (-)-epicatechin and p-coumaric acid were the next most abundant phenolics. The highest level of trans-resveratrol among the red wines was found Buzbagi (Bogazkere-Okuzgozu). The red wines were analyzed for total polyphenol content (TP) by Folin-Ciocalteu (FC) method, using gallic acid as standard. Antioxidant activities (M) of the red wines were measured using the DPPH (2,2-diphenyl-1-picrylhydrazyl) assay. There was a very high correlation between AA and TP in all of the wines tested. (C) 2011 Elsevier Ltd. All rights reserved.Öğe Social phobia and other psychiatric problems in children with strabismus(Canadian Ophthal Soc, 2011) Cumurcu, Tongabay; Cumurcu, Birgul Elbozan; Ozcan, Ozlem; Demirel, Soner; Duz, Cem; Porgali, Esra; Doganay, SelimObjective: To investigate the rate of social phobia, anxiety, depression, and other psychiatric problems in children with strabismus. Design: Prospective, cross-sectional, case-control study. Participants: Forty-two children with strabismus and 47 control subjects 8-13 years of age were enrolled in this study. Methods: After the ophthalmologist's examination, all cases were assessed by a psychiatrist based on the structured interview technique of Schedule for Affective Disorders and Schizophrenia for School-Aged Children-Present and Lifetime Version (Kiddie-SADS-PL). The Screen for Child Anxiety Related Emotional Disorders (SCARED) was administered to each subject to evaluate social phobia. All participants completed the Children's Depression Inventory (CDI). Results: Age as well as sex and income were comparable between the strabismus patients and control groups. Social phobia was diagnosed in 8 (19.04%) of the 42 strabismic children and in 1 (2.12%) of the control subjects. The CDI and SCARED (total score, social phobia, separation anxiety) scores of strabismus patients were significantly higher than the control group (p = 0.001, p = 0.004, p = 0.0001, p = 0.05, respectively). Conclusions: A relationship between strabismus in children and social phobia, depression, and anxiety on a symptom basis was underlined by our data.
