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    Importance of hair trace metal analysis after pectus bar implantation
    (2021) Akkus, Murat; Seyrek, Yunus; Yoruc Hazar, Afife Binnaz; Sahin, Fikrettin
    Aim: This study aimed to determine if there was a significant increase in scalp hair levels of trace metals in children with implanted pectus bars and investigate previously unreported hair metal levels after minimally invasive repair of pectus excavatum (MIRPE). Materials and Methods: Between November 2013 and December 2019, we prospectively collected scalp hair samples from patients before MIRPE and prior to pectus bar explantation (1 day before bar removal) to evaluate trace metal levels following the implantation of metal bars. Two study groups were involved: a group of consecutive patients who underwent pectus bar removal 2–5 years after MIRPE (study group, n = 97), and a group including the same patients (control group, n = 97) prior to MIRPE.Results: The mean patient age was 13.3 ± 2.7 years (range, 11–19 years) preoperative and 16.4 ± 2.3 years (range 14–23 years) postoperative; 64% of the patients were male (n = 62). Statistically significant increases in all studied trace metal levels (chrome, iron, nickel, and molybdenum) were identified in the intergroup comparison of hair trace metal analysis.Conclusion: The results of this prospective study show that hair is a good biological marker for the monitoring and study of trace metals released from pectus bars. This simple assessment may be useful for the screening of trace metal increase due to the pectus bar. When metal allergy is suspected during follow-up after MIRPE, the availability of preoperatively collected hair samples will be quite beneficial.
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    Improved treatment of recurrent aphthous stomatitis with sodium pentaborate pentahydrate (NaB)
    (2021) Sezim Safak, Ayse; Sahin, Fikrettin
    Aim: Chlorhexidine (CHX) and benzydamine hydrochloride (B-HCl) combination treatment (Kloroben™ spray) was compared with sodium pentaborate pentahydrate (NaB, also called boron) treatment with regard to their effects in the recovery of recurrent aphthous stomatitis (RAS). Materials and Methods: We assessed the treatment results by evaluating ulcer radius and VAS results. Seventy-one patients with clinically diagnosed minor RAS were selected for the study based on inclusion and exclusion criteria. Group 1 was treated with a 3% solution of NaB and group 2 was treated with Kloroben spray (45 mg benzydamine HCl and 36 mg chlorhexidine gluconate) for 1 week. Results: After the treatments, there were statistically significant decreases in VAS scores in both Group 1 (p0.001) and Group 2 (p0.001). We found that the decrease in VAS score was greater in Group 1 than in Group 2 (p0.001). Similarly, there were statistically significant decreases in ulcer diameters for both groups. The ulcer diameter reduction was also greater in Group 1 than Group 2 (p0.001). Conclusions: When the specifications of NaB and studies that report its effects are considered, it may be concluded that NaB works well in the healing process of oral aphthae. Our findings show that NaB is better than CHX for the treatment of RAS, which is demonstrated by greater reduction of ulcer diameter and pain score in the NaB group.
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    Schiff base-Poloxamer P85 combination demonstrates chemotherapeutic effect on prostate cancer cells in vitro
    (Elsevier France-Editions Scientifiques Medicales Elsevier, 2017) Demirci, Selami; Dogan, Aysegul; Turkmen, Nese Basak; Telci, Dilek; Rizvanov, Albert A.; Sahin, Fikrettin
    Prostate cancer is a multistep and complicated cancer type that is regulated by androgens at the cellular level and remains the second commonest cause of death among men. Discovery and development of novel chemotherapeutic agents enabling rapid tumor cell death with minimal toxic effects to healthy tissues might greatly improve the safety of chemotherapy. The present study evaluates the anti-cancer activity of a novel heterodinuclear copper(II) Mn(II) complex (Schiff base) in combination with poly(ethylene oxide) and poly(propylene oxide) block copolymer (Pluronic) P85. We used assays for cell proliferation, apoptosis, cell migration and invasion, DNA binding and cleavage to elucidate the molecular mechanisms of action, in addition to the antiinflammatory potency of the new combination. The combined treatment of Schiff base and P85 lead to a remarkable anti-cancer effect on prostate cancer cell lines. Cell proliferation was inhibited in Schiff base-P85 treatment. The activity of this formulation is on DNA binding and cleavage and prevents inflammation in in vitro conditions. This is the first study presenting the anti-cancer activity of the present Schiff base derivative and its combination with P85 to treat prostate cancer in vitro. (C) 2016 Elsevier Masson SAS. All rights reserved.
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    Visualization and characterization ofEnterococcus faecalisbiofilm structure in bovine dentin using 2D and 3D microscopic techniques
    (Springer, 2021) Keles, Ali; Keskin, Cangul; Kalkan, Melis; Yakupogullari, Yusuf; Gul, Mehmet; Aydemir, Hikmet; Sahin, Fikrettin
    Bacterial biofilms are related to various dental and periodontal infectious diseases, and the characterization of this biological structure with micro-computed tomography (micro-CT) may offer valuable information for clinical and research applications. In this study, we aimed to develop a model to visualize three-dimensionally the biofilm structure on dentin using micro-CT. Dentin blocks were prepared and incubated in tryptic soy broth withEnterococcus faecalis(ATCC 29212). The control group did not receive any staining procedure, while groups 1 and 2 were stained with 100% and 50% barium sulfate, respectively. Transmission electron microscopy (TEM) and confocal laser scanning microscopy (CLSM) were used to detect biofilm formation, barium sulfate penetration, and microbial cell density in the biofilm. Micro-computed tomography (micro-CT) (SkyScan 1172, Bruker Co., Belgium) was used to visualize biofilm formation on the dentin blocks. Biofilm thicknesses were measured from 10 different locations on the specimen surfaces, using CTAn v.1.14.4 software. Obtained data were statistically analyzed using Kruskal-Wallis and Dunn's tests. TEM photomicrographs showed that barium sulfate could penetrate the biofilm structure. CLSM analysis showed that viable and total cell densities were similar between the control and barium sulfate-treated groups (P > 0.05), indicating barium sulfate had no significant influence on cell density. In barium sulfate-treated blocks, biofilm could be discriminated from the dentin, and its thickness could be measured with micro-CT. This study showed that bacterial biofilm on dentin could be characterized by micro-CT after barium sulfate staining without causing any significant side effect on viable and total cell densities.