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Öğe The Acute Effect of Humic Acid on Iron Accumulation in Rats(Humana Press Inc, 2016) Cagin, Yasir Furkan; Sahin, N.; Polat, A.; Erdogan, M. A.; Atayan, Y.; Eyol, E.; Bilgic, Y.Free iron leads to the formation of pro-oxidant reactive oxygen species (ROS). Humic acids (HAs) enhance permeability of cellular wall and act as a chelator through electron transferring. This study was designed to test chelator effect of HA on iron as well as its anti-oxidant effect against the iron-induced hepatotoxicity and cardiotoxicity. The rats used were randomly divided into four groups (n = 8/group): group I (the control group); group II (the HA group), humic acid (562 mg/kg) was given over 10 days by oral gavage; group III (the iron group), iron III hydroxide polymaltose (250 mg/kg) was given over 10 days by intraperitoneal route; and group IV (the HA plus iron group), received the iron (similar to group II) plus humic acid (similar to those in groups II and III) group. Blood and two tissue samples both from liver and heart were obtained for biochemical and histopathological evaluations. Iron deposition, the iron-induced hepatotoxicity, and cardiotoxicity were demonstrated by histopathological and biochemical manner. However, no significant differences were observed in the serum biochemical values and the histopathological results among the iron and the HA plus iron groups in the liver tissue but not in the heart tissue. The protective effects of humic acid against iron-induced cardiotoxicity were shown but not against hepatotoxicity in our study.Öğe Analysis of E6/E7 mRNA gene expression by means of NucliSENS EasyQ (NASBA) technique in patients suffering from cervical dysplasia with HPV positive(7847050 Canada Inc, 2017) Ciplak, B.; Yilmaz, E.; Otlu, B.; Coskun, E. I.; Sahin, N.; Tuncay, G.Objective: In this study, the authors aimed at examining E6/E7 mRNA gene expression by means of NucliSENS EasyQ (NASBA) in patients in which HPV infection was detected and diagnosed with cervical dysplasia. Materials and Methods: This study included 77 patients diagnosed with cervical dysplasia. The patients were grouped based on cervico-vaginal smear anomalies. Digene HC2 DNA Collection Device transport medium was used for taking and keeping cervical samples, QIA symphony SP device, and QIAsymphony DSP AXpH DNA kit were used for HPV DNA extraction from cervical samples kept in transport medium, HPV Q24 complete kit, RotorGene and PyroMark Q24 for detection of HPV-DNA and determination of HPV types and NucliSENS Easy Q Genetic Analyzer technique for determination of E6/E7 gene expression in HPV positive samples. SPSS (Statistical Package for Social Sciences) Version 22.0 was employed in statistical evaluation of the research date. Results: The most common HPV was considered as type 16 in this study. When pathology specimens of patients in whom dysplasia was detected in their cervical biopsies were examined in terms of HPV E6/E7 mRNA expression, a statistically significant difference was found between the normal and pathology groups (p < 0.05). Conclusion: Today, the presence of HPV infection in etiology of cervical smear pathologies and dysplasia is undisputedly accepted. The main determinant factor of HPV virulence is the frequency of E6 and E7 gene expressions existing in the DNA structure and responsible for virulence. NucliSENS_Ea_syQ (NASBA) is a technique employed to analyze mRNA gene expression in an accurate, reliable, and rapid way. Parallel to the conclusions of studies in the literature, frequency of E6/E7 gene expression increases in proportion to the increasing dysplasia degree of cervical pathologies in the present study as well.Öğe Analysis of e6/e7 mrna gene expression by means of nuclisens easyq (nasba) technique in patientssuffering from cervical dysplasia with hpv positive(I r o g canada, ınc, 4900 cote st-luc, apt#212, montreal, quebec h3w 2h3, canada, 2017) Ciplak, B.; Yilmaz, E.; Otlu, B.; Coskun, E. I.; Sahin, N.; Tuncay, GObjective: In this study, the authors aimed at examining E6/E7 mRNA gene expression by means of NucliSENS EasyQ (NASBA) in patients in which HPV infection was detected and diagnosed with cervical dysplasia. Materials and Methods: This study included 77 patients diagnosed with cervical dysplasia. The patients were grouped based on cervico-vaginal smear anomalies. Digene HC2 DNA Collection Device transport medium was used for taking and keeping cervical samples, QIA symphony SP device, and QIAsymphony DSP AXpH DNA kit were used for HPV DNA extraction from cervical samples kept in transport medium, HPV Q24 complete kit, RotorGene and PyroMark Q24 for detection of HPV-DNA and determination of HPV types and NucliSENS Easy Q Genetic Analyzer technique for determination of E6/E7 gene expression in HPV positive samples. SPSS (Statistical Package for Social Sciences) Version 22.0 was employed in statistical evaluation of the research date. Results: The most common HPV was considered as type 16 in this study. When pathology specimens of patients in whom dysplasia was detected in their cervical biopsies were examined in terms of HPV E6/E7 mRNA expression, a statistically significant difference was found between the normal and pathology groups (p < 0.05). Conclusion: Today, the presence of HPV infection in etiology of cervical smear pathologies and dysplasia is undisputedly accepted. The main determinant factor of HPV virulence is the frequency of E6 and E7 gene expressions existing in the DNA structure and responsible for virulence. NucliSENS_Ea_syQ (NASBA) is a technique employed to analyze mRNA gene expression in an accurate, reliable, and rapid way. Parallel to the conclusions of studies in the literature, frequency of E6/E7 gene expression increases in proportion to the increasing dysplasia degree of cervical pathologies in the present study as well.Öğe BRAF GENE MUTATIONS AND PROGNOSTIC SIGNIFICANCE OF CLINICAL FINDINGS IN CASTLEMAN DISEASE(Ferrata Storti Foundation, 2016) Turker, M.; Subari, S.; Keklik, M.; Dal, M. S.; Sahin, N.; Comert, M.; Alanoglu, G.[Abstract Not Available]Öğe Breast metastasis from ovarian serous micropapillary carcinoma(Springer, 2016) Alcatli, A. N.; Sahin, N.; Kaplan, N.; Gokce, H.[Abstract Not Available]Öğe A CASE OF CONFINED PLACENTAL MOSAICISIVI WITH TRISOMY 15 ASSOCIATED WITH TURNER SYNDROME(Medecine Et Hygiene, 2016) Ekici, C.; Sahin, Y.; Yaykasli, K. O.; Melekoglu, R.; Sahin, N.; Yuksel, S.A case of confined placental mosaicism with trisomy 15 associated with Turner syndrome: We here present a rare case of a Turner syndrome with mosaic trisomy 15 identified on chorionic villous sampling (CVS). Although there are several reports in the literature indicating confined placental mosaicism (CPM), counseling parents of a fetus with trisomy 15 mosaicism at CVS remains difficult because of the phenotypic variability. To illuminate that condition an amniocentesis or cord blood study should be offered in conjunction with genetic counseling.Öğe Comparison of Esomeprazole and Lansoprazole in Eradicating H. pylori Among Children(Wiley-Blackwell Publishing, Inc, 2009) Ugras, M.; Sahin, N.[Abstract Not Available]Öğe Diffusion-weighted imaging of the liver in assessing chronic liver disease: effects of fat and iron deposition on ADC values(Verduci Publisher, 2022) Kahraman, A. S.; Kahraman, B.; Ozdemir, Z. M.; Karaca, L.; Sahin, N.; Yilmaz, S.OBJECTIVE: This study was de-signed to evaluate whether fat and iron affect the apparent diffusion coefficient (ADC) values of the liver parenchyma in the settings of fibro-sis and inflammation.PATIENTS AND METHODS: We evaluated the diffusion-weighted images (DWIs) of 58 pa-tients with chronic liver disease and 48 con-trol subjects. Liver specimens of patients were assessed for fibrosis, necroinflammation, iron, and steatosis. Liver ADCs, spleen ADCs, and normalized liver ADCs (defined as the ratio of the liver ADC to spleen ADC) values were an-alyzed after stratifying patients with either fi-brosis stages or histology activity index (HAI) scores. The relationship between ADC values and histopathological findings was studied us-ing multiple linear regression analysis.RESULTS: The median liver and normalized liver ADC values were significantly lower in higher stages of fibrosis and HAI scores. Com-pared to the control group, patients with the highest stages of fibrosis and inflammation had significantly higher spleen ADCs. The ef-fect of the fibrosis stage on liver ADC and nor-malized liver ADC values was significant in the setting of inflammation, whereas the degree of steatosis and iron grade did not affect these ADC values.CONCLUSIONS: ADC values can distinguish both later stages of liver fibrosis and inflam-mation. There is no significant effect of fat and iron on ADC values. Therefore, DWI may be re-liable in evaluating liver fibrosis and inflamma-tion.Öğe The effect of Cirsium arvense extract on antioxidant status in quail(Taylor & Francis Ltd, 2013) Orhan, C.; Sahin, N.; Akdemir, F.; Markiewicz-Zukowska, R.; Borawska, M. H.; Isidorov, V. A.; Hayirli, A.1. The herb Creeping Thistle, Cirsium arvense (C. arvense), has been used in folk medicine due to its antioxidant and anti-inflammatory effects.2. The aim of this study was to investigate the effects of dietary C. arvense extract supplementation on performance, egg quality, nutrient digestibility and antioxidant status in quail.3. Quails (n=150) were allocated randomly to one of the three dietary treatments: basal diet and basal diet enriched with 100 and 200mgC. arvense extract per kg diet.4. Dietary enrichment with C. arvense extract altered neither performance and egg quality parameters nor nutrient digestibility. Although there were no changes in concentrations of vitamin A and E in serum, liver and egg yolk, supplemental C. arvense extract decreased MDA concentrations in serum, liver and egg yolk by 39.3, 40.5 and 51.5%, respectively, in a dose-response manner. As supplemental C. arvense extract increased to 200mg/kg, the activity of hepatic SOD, CAT and GSH-Px increased by 14.5, 17.4 and 35.5%, respectively.5. Addition of C. arvense extract up to 200mg to per kg diet enhanced antioxidant status in laying quail and their eggs, without affecting performance and other egg quality parameters.6. Future studies are needed to elucidate the mechanism behind the antioxidant effects of C. arvense extract.Öğe Effects of dietary arginine silicate inositol complex on mineral status in rainbow trout (Oncorhynchus mykiss)(Wiley, 2008) Kucukbay, F. Z.; Yazlak, H.; Sahin, N.; Akdemir, F.; Orhan, C.; Juturu, V.; Sahin, K.Arginine silicate inositol complex (ASI; arginine 49.47 g kg(-1), silicon 8.2 g kg(-1), inositol 25 g kg(-1)), a novel composition that is a bioavailable source of silicon and arginine, has potential benefits for vascular and bone health. We have previously reported that bone mineral content increased and the amount of Ca, P, Mg and Mn in the excreta decreased in poultry with ASI supplementation. In the present study, the effect of ASI supplementation at various levels (0, 500, 1000 mg kg(-1) ASI) on growth, feed intake, feed conversion ratio (FCR) and concentrations of body elements, operculum bone ash and activity of serum alkaline phosphatase (ALP) in rainbow trout was evaluated. Ninety 0+ year-old rainbow trout with initial average weight of 50 +/- 3 g were randomly assigned to three treatment groups, three replicates of 10 fish each. The fish were fed either a basal diet or the basal diet supplemented with either 500 or 1000 mg of ASI. Body weight gain (P = 0.25), feed intake (P = 0.36) and feed efficiency (P = 0.42) were not signifcantly influenced by the dietary ASI supplementation. Per cent operculum bone ash (634 g kg(-1) versus 558 g kg(-1), P = 0.001) and ALP activity (112 UL-1 versus 92 UL-1, P = 0.001) linearly increased as dietary ASI supplementation increased. Increasing dietary ASI supplementation linearly increased serum and whole body Ca (P = 0.01), P (P = 0.01), Mg (P = 0.05; P = 0.001) Mn (P = 0.05; P = 0.01) and Zn (P = 0.01; P = 0.02) concentrations respectively. In conclusion, ASI supplementation to the basal diet significantly improved operculum bone ash and whole body mineral content in rainbow trout and did not impact feed consumption, weight gain or FCR.Öğe The effects of dietary organic or inorganic selenium in rainbow trout (Oncorhynchus mykiss) under crowding conditions(Wiley, 2009) Kucukbay, F. Z.; Yazlak, H.; Karaca, I.; Sahin, N.; Tuzcu, M.; Cakmak, M. N.; Sahin, K.In the present study, the effects of different sources of selenium (Se; sodium selenite or selenomethionine) supplementation on the growth and serum concentrations of oxidative stress markers [malondialdehyde (MDA), 8-isoprostane, glutathione peroxidase (GSH-Px) activity] and muscle Se, MDA and heat shock protein 70 (Hsp70) levels in rainbow trouts were evaluated. The fish (n = 360; 0 + years old) with initial average weight of 20 +/- 0.8 g were randomly assigned to 12 treatment groups consisting of 3 replicates of 10 fish each in a 2 x 2 x 3 factorial arrangement of treatments (stocking densities, Se sources, Se levels). The fish were kept at low (25 kg m-3) or high (100 kg m-3) stocking densities and fed a basal (control) diet or the basal diet supplemented with either 0.15 or 0.30 mg of Se kg-1 of diet from two different forms: sodium selenite or selenomethionine. High stocking density decreased weight gain, feed intake and feed conversion ratio (FCR) when basal diet was fed (P = 0.001). A linear increase in feed intake and weight gain and improvement in FCR were found in sodium selenite (P = 0.01)- or selenomethionine (P = 0.001)-supplemented fish reared under crowding conditions. Serum and muscle Se levels and serum GSH-Px activity increased (P = 0.001) linearly, whereas serum and muscle MDA concentrations and serum 8-isoprostane decreased linearly as dietary sodium selenite (P = 0.01) or selenomethionine (P = 0.001) supplementation increased. Selenomethionine and sodium selenite supplementation decreased Hsp70 in the muscle of fish reared under crowding conditions (P < 0.05). Supplementation with Se improved growth and antioxidant status of fish and the effects of selenomethionine were relatively greater than sodium selenite in the crowded groups. Results suggest that crowding conditions cause significant detrimental effects in rainbow trout indicated by increased oxidative stress, reduced feed intake and body weight gain. It also indicates that dietary Se supplementation offers a feasible way of reducing the losses in performance of rainbow trout reared under crowding conditions. Selenomethionine seems to be more effective than sodium selenite and the higer dose in the present study also seems to be more effective than the lower dose.Öğe The effects of Teucrium polium on ionizing radiation-induced intestinal damage in rats(Univ Catholique Louvain-Ucl, 2011) Demirel, U.; Harputluoglu, M. M. M.; Us, S. B.; Kaya, E.; Sahin, N.; Aydin, N. E.; Gursoy, S.Background and study aims : Oxidative stress plays an important role in development of intestinal injury after abdomino-pelvic radiation therapy. Teucrium poliuin (TP) is a medicinal plant which has antioxidant and anti-inflammatory properties. The aim of this study was to investigate the effect of TP on radiation-induced intestinal oxidative damage in rats. Materials and methods : Group 1 (n = 8), the control group; Group 2 (n = 8), the RAD (radiation) group in which each rat received a single whole-body 800 cGy radiation performed with a LINAC; Group 3 (n = 8), the RAD + TP group in which rats were exposed to radiation as in Group 2, followed by intragastric administration of 0.5 g/kg/daily TP extract for 7 consecutive days; and Group 4 (n = 8), the TP group, rats received only intragastric TP for 7 days. Results : Radiation led to intestinal damage, which was accompanied by an increase in intestinal thiobarbituric-acid-reactive substances (TBARS) and myeloperoxidase (MPO) levels, and a decrease in reduced glutathione (GSH) levels. Although TP significantly decreased intestinal MPO levels and inflammation scores, it neither reverted intestinal TBARS and GSH levels nor ameliorated other histological parameters of the disease. Conclusions : Our results suggest that TP reduces inflammation but does not ameliorate the increased oxidative stress conditions in radiation-induced intestinal damage in rats. (Acta gastroenterol. belg., 2011, 74, 491-496).Öğe Effects of the supplemental chromium form on performance and oxidative stress in broilers exposed to heat stress(Elsevier, 2017) Sahin, N.; Hayirli, A.; Orhan, C.; Tuzcu, M.; Akdemir, F.; Komorowski, J. R.; Sahin, K.This experiment was conducted to investigate effects of the organic complex form of supplemental chromium (Cr) on performance, oxidative stress markers, and serum profile in broilers exposed to heat stress (HS). A total of 1,200 10-day-old boilers (Ross-308) was divided into one of the 6 treatments (2 environmental temperatures x 3 diets with different Cr forms). The birds were kept in temperature-controlled rooms at either 22 +/- 2A degrees C 24 h/d (thermoneutral, TN group) or 34 +/- 2A degrees C for 8 h/d, 08:00 to 17:00 h, followed by 22A degrees C for 16 h (HS group) and fed either a basal diet (C) or the basal diet supplemented with Cr (200 mu g/kg) through 1.600 mg of CrPic (12.43% Cr) and 0.788 mg of CrHis (25.22% Cr). Feed intake and body weight were recorded weekly. After cervical dislocation, liver samples were harvested to analyze Cr concentration and glucose transporter-2,4 (GLUT-2,4) expression. The breast meat also was sampled for the concentration of Cr and expressions of nuclear factor erythroid 2-related factor 2 (Nrf2) and nuclear factor kappa B (NF-kappa B). Data were analyzed by 2-way ANOVA. Heat stress caused depressions in feed intake (12.1%) and weight gain (21.1%) as well as elevations in feed conversion (11.2%) and abdominal fat (32.8%). It was also associated with depletion of Cr reserves and increases in serum concentrations of glucose, cholesterol, creatine, and enzymes. Exposure to HS was accompanied by suppression of the expressions of Nrf2 and GLUT-2 in muscle and GLUT-4 in the liver and amplification of the expression of NF-kappa B in muscle. Both Cr sources partially alleviated detrimental effects of HS on performance and metabolic profile. The efficacy of Cr as CrHis was more notable than Cr as CrPic, which could be attributed to higher bioavailability. In conclusion, CrHis can be added into the diet of broilers during hot seasons to overcome deteriorations in performance and wellbeing related to oxidative stress.Öğe Isokinetic evaluation of knee extensor/flexor muscle strength in Behcet's patients(Publisaude-Edicoes Medicas Lda, 2015) Durmus, B.; Emre, S.; Sahin, N.; Karincaoglu, Y.; Dogan, E.; Baysal, O.; Ersoy, Y.Background: Behcet's disease (BD) is an idiopathic, multisystemic, progressive disease. The purpose of this study is to compare the knee flexor and extensor isokinetic muscle strengths of Behcet's patients with that of healthy subjects. Methods: Twenty-five (13 male and 12 female) patients with BD and 25 (15 male and 10 female) healthy individuals were included in the study Velocities of 90 degrees/sec, 120 degrees/sec, and 150 degrees/sec were used for the isokinetic muscle strength testing. Patients with active inflammatory knee arthritis were excluded. Peak torque (Nm) and peak torque adjusted to body weight (%) were taken into consideration for comparison between study groups. Results: Compared to healthy controls, there was a statistically significant decrease in both the bilateral knee extensor and flexor muscle isokinetic peak torques (Nm) as well as the peak torques adjusted to body weight (%) at velocities of 90 degrees/sec, 120 degrees/sec and 150 degrees/sec in patients with BD (p < 0.05). However, there was no significant difference in the agonist-antagonist ratio of the isokinetic peak torques of knee muscles between the two groups. Conclusion: In light of these findings, we have concluded that both knee flexor and extensor isokinetic muscle strengths are lower in BD. We therefore recommend careful monitoring of patients with BD in temis of muscle strength.Öğe Isolated recurrence of early-stage cervical cancer in the abdominal wall with suboptimal surgery(I r o g canada, ınc, 4900 cote st-luc, apt#212, montreal, quebec h3w 2h3, canada, 2018) Yilmaz, E.; Coskun, E., I; Koc, C.; Sahin, N.; Isik, B.; Ciplak, B.In this report, the authors present the case of a 47-year-old woman with an isolated recurrence of early-stage cervical cancer treated with suboptimal surgery of the abdominal wall and that subsequently underwent radiotherapy.Öğe Isolated recurrence of early-stage cervical cancer in the abdominal wall with suboptimal surgery(I R O G Canada, Inc, 2018) Yilmaz, E.; Coskun, E., I; Koc, C.; Sahin, N.; Isik, B.; Ciplak, B.In this report, the authors present the case of a 47-year-old woman with an isolated recurrence of early-stage cervical cancer treated with suboptimal surgery of the abdominal wall and that subsequently underwent radiotherapy.Öğe Longstanding skin ulcers due to Mycobacterium tuberculosis in a healthy man(Malaysian Soc Parasitology Tropical Medicine, 2010) Sahin, N.; Aydin, N. E.; Senol, M.; Ozerol, I. H.Cutaneous tuberculosis, a rare form of extrapulmonary tuberculosis, has a wide variety of clinical presentations and continues to be one of the most important dermatological diseases in developing countries. The sites of predilection are neck, supraclavicular region, axilla, and groin. Single or multiple cutaneous and subcutaneous nodules first appear and breakdown later resulting in undermined ulcers with a purulent discharge, sinuses, and disfiguring scars. We report a multifocal case of scrofuloderma in a 47-year-old immunocompetent man treated successfully with four antituberculotic drugs, albeit lately due to the loss of awareness to the disease.Öğe MPV, NLR, and platelet count: new hematologic markers in diagnosis of malignant ovarian tumor(I R O G Canada, Inc, 2017) Yilmaz, E.; Coskun, E. I.; Sahin, N.; Ciplak, B.; Ekici, K.Objective: Evaluation of mean platelet volume (MPV), neutrophil to lymphocyte ratio (NLR), and platelet count in the detection of malignant and benign ovarian tumors. Material and Methods: Sixty-six patients admitted to the present clinic over three years were included in the study. Patients diagnosed with ovarian cancer were grouped into the areas of epithelial and granulosa cell tumor diagnosis. The values were compared to patients with benign cystic structure. Patients' preoperative hematologic parameters and their values four weeks postoperatively were analyzed. Statistical analyses were performed with SPSS software. Results: MPV, NLR, and platelet count were observed at a higher rate as statistically significant in patients diagnosed with malignant ovarian cancer, compared to those with benign adnexal mass. Conclusion: The hematological parameters such as MPV, NLR, and platelet count in the detection of malignant ovarian tumors have been evaluated as useful new markers.Öğe Nuclear factor-kappa beta pathway and endometrial cancer: a pilot study(I R O G Canada, Inc, 2017) Yilmaz, E.; Coskun, E. I.; Gul, M.; Sahin, N.; Tuncay, G.; Simsek, Y.Objective: Examination of the role of nuclear factor-kappa beta (NF-kappa B) expression in the etiopathogenesis of endometrial cancer, by means of the immunohistochemical method. Materials and Methods: Patients who applied to participate in the study at the clinic were grouped into three categories: those diagnosed with benign endometrial pathology, those with endometrial hyperplasia, and those with endometrial cancer. NF-kappa B analysis was conducted in the endometrial tissues of the patients' paraffin blocks by means of the immunohistochemical method. For objective assessment purposes, the H score of each patient was calculated. SP SS 15.0 program was employed for statistical analysis. Results: The average H score of the first group, comprising benign endometrial pathologies, was 102.4 +/- 85.9, that of the hyperplasia group was 143.6 +/- 122.4, and that of the cancer group was 276.8 +/- 61.8. The average values of groups 1 and 2 were similar (p = 0.349); however, the third group's average H score was significantly higher (p < 0.001). Conclusion: NF-kappa B, which is a critical mediator in the inflammation process, might be related to the development of premalign and malign endometrial changes.Öğe Pathologic characteristics of resected hepatocellular carcinomas(Springer, 2016) Nurkabul, Z.; Samdanci, E. Turkmen; Aydin, N. E.; Akatli, A. N.; Sahin, N.; Dagli, A. F.; Cakir, E.[Abstract Not Available]
