Yazar "Sahin-Bolukbasi, Serap" seçeneğine göre listele

Listeleniyor 1 - 6 / 6
  • Küçük Resim Yok
    Öğe
    Novel Ag(I)-NHC complex: synthesis, in vitro cytotoxic activity, molecular docking, and quantum chemical studies
    (Walter De Gruyter Gmbh, 2022) Serdaroglu, Goncagul; Sahin, Neslihan; Sahin-Bolukbasi, Serap; Ustun, Elvan
    The importance of organometallic complexes in cancer biology has attracted attention in recent years. In this paper, we look for the in vitro cytotoxic capability of novel benzimidazole-based N-heterocyclic carbene (NHC) precursor (1) and its Ag(I)-NHC complex (2). For this purpose, these novel Ag(I)-NHC complex (2) was characterized by spectroscopic techniques (H-1, C-13{H-1} nuclear magnetic resonance (NMR), and Fourier-transform infrared spectroscopy (FT-IR)). Then, in vitro cytotoxic activities of NHC precursor (1) and Ag(I)-NHC complex (2) were investigated against MCF-7, MDA-MB-231 human breast, DU-145 prostate cancer cells, and L-929 healthy cells using MTT assay for 24, 48, and 72 h incubation times. Ag(I)-NHC complex (2) showed promising in vitro cytotoxic activity against all cell lines for three incubation times, with IC50 values lower than 5 mu M. It was also determined that (NHC) precursor (1) were lower in vitro cytotoxic activity than Ag(I)-NHC complex (2) against all cell lines. Selectivity indexes (SIs) of Ag(I)-NHC complex (2) against cancer cells were found higher than 2 for 24 and 48 h incubation time. Besides, the electronic structure and spectroscopic data of the newly synthesized precursor and its Ag-complex have been supported by density functional theory (DFT) calculations and molecular docking analysis. After, the anticancer activity of these compounds has been discussed considering the results of the frontier molecular orbital analysis. We hope that the obtained results from the experiments and computational tools will bring a new perspective to cancer research in terms of supported by quantum chemical calculations.
  • Küçük Resim Yok
    Öğe
    Rhodium(i) N-heterocyclic carbene complexes: synthesis and cytotoxic properties
    (Royal Soc Chemistry, 2021) Slimani, Ichraf; Sahin-Bolukbasi, Serap; Ulu, Mustafa; Evren, Enes; Gurbuz, Nevin; Ozdemir, Ilknur; Hamdi, Naceur
    Rhodium(i) complexes bearing N-heterocyclic carbene (NHC) ligands have been widely used in catalytic chemistry, but there are very few reports of biological properties of these types of complexes. A series of benzimidazolium salts and their [RhCl(NHC)(COD)] complexes were synthesized. The obtained complexes were synthesized and characterized by elemental analysis, FT-IR, H-1 and C-13 NMR. All compounds were screened for in vitro cytotoxic activities against a panel of human cancer cells (HT-29 colon, Ishikawa endometrial, and U-87 glioblastoma) using the MTT assay for 48 h of incubation time. Mouse fibroblast cells (L-929) were used as healthy cells. Complexes had exhibited significantly higher cytotoxic activity towards cancer cells than their ligands and complex 2b showed the most selective cytotoxic activity against HT-29 cancer cells (SI;7.05) and Ishikawa cancer cells (SI; more than 9.8). The complexes showed strong in vitro cytotoxic activity against cancer cells, with IC50 values of lower than 10 mu M (except 2a against HT-29 (12.8 mu M) and 2b against U-87 (11.1 mu M)). All complexes (2a-d) showed the highest in vitro cytotoxic activity against Ishikawa endometrial cancer cells with IC50 values of 2.93 +/- 0.06, <1, 2.60 +/- 0.05, and 2.85 +/- 0.06 mu M, respectively. Complexes were found to be highly cytotoxic against HT-29, Ishikawa, and U-87 cancer cells compared to the anticancer agents, cisplatin and 5-FU.
  • Küçük Resim Yok
    Öğe
    Synthesis and antitumor activity of new silver(I)-N-heterocyclic carbene complexes
    (Taylor & Francis Ltd, 2019) Sahin, Neslihan; Sahin-Bolukbasi, Serap; Marsan, Halis
    In this study, two novel benzimidazole-based N-heterocyclic carbene ligands (1a-b) and their silver(I) complexes (2a-b) were synthesized. All new compounds were characterized by FT-IR, LC-MS, H-1 NMR, and C-13 NMR spectroscopies. The in vitro antitumor activities of NHC ligands (1a-b) and their silver(I) complexes (2a-b) against DU-145 human prostate cancer cells, MDA-MB-231 and MCF-7 human breast cancer cells and L-929 (normal cells adipose from mouse) were also determined using MTT analysis for 24 h, 48 h, and 72 h. The results showed that while NHC ligands did not have in vitro antitumor activity on MCF-7, MDA-MB-231 and DU-145 cells, Ag(I)-NHC complexes have in vitro antitumor activities. The in vitro antitumor activity of 2a was found to be lower than that of 2b. Ag(I)-NHC complexes were observed to have higher IC50 values for non-cancerous cell lines than cancer cells.
  • Küçük Resim Yok
    Öğe
    Synthesis, characterization and anticancer activity of allyl substituted N-Heterocyclic carbene silver(I) complexes
    (Elsevier Science Bv, 2019) Sahin, Neslihan; Sahin-Bolukbasi, Serap; Tahir, Muhammad Nawaz; Arici, Cengiz; Cevik, Esranur; Gurbuz, Nevin; Ozdemir, Ismail
    Metal N-heterocyclic carbene (NHC) complexes have attracted considerable attention in biological fields for their potential applications in cancer and antimicrobial therapies. In this study, four new benzimidazole-based N-heterocyclic carbene salts (la-d) and their silver (I) complexes (2a-d) were synthesized. All new compounds were characterized by elemental analysis, FT-IR, H-1 NMR and, C-13 NMR spectroscopy. Additionally, single crystal structural studies for complex 2d show that the benzene rings (C9-C14) and the central benzimidazole ring system make dihedral angles of 83.58(13)degrees. The Ag-Cl and Ag-C single bond lengths are 2.3267(8) and 2.087(3) angstrom, respectively. The C-Ag-Cl bond angle is 175.20(7)degrees. The prop-1-ene moiety attached at the second N-atom of benzimidazole is disordered at two set of sites with an occupancy ratio of 0.592(6): 0.408(6). There is one intramolecular hydrogen bond interaction between C22A-H22A... N2. The salts and Ag-complexes were further evaluated for their in vitro anticancer activities against DU-145 human prostate cancer cells, MCF-7, MDA-MB-231 human breast cancer cells and L-929 non-cancer cell for 24 h, 48 h and 72 h using the MTT assay. The Ag(I)-NHC complexes (2a-d) showed a dose and time-dependent cytotoxic activity against all cell lines. The IC(50)s for all Ag(I) NHC complexes lower than 1 mu M for 72 h time points on cancer cells. The results showed that complex 2d exhibited the highest activity against all cancer cell lines studied. Further, the complexes had relatively higher cytotoxicity to cancer cells than to non-cancer cell lines. (C) 2018 Elsevier B.V. All rights reserved.
  • Küçük Resim Yok
    Öğe
    Synthesis, characterization, and investigation of antiproliferative activity of novel Ag (I)-N-Heterocyclic Carbene (NHC) compounds
    (Elsevier, 2020) Cevik-Yildiz, Esranur; Sahin, Neslihan; Sahin-Bolukbasi, Serap
    The aim of this study was to present the synthesis, characterization, and investigation of the antiproliferative activity of new metal N-Heterocyclic Carbene (NHC) salts (1a-c) and their Ag(I) complexes (2a-c). All synthesized compounds were characterized using elemental analysis, LC-MS, FT-IR,H- 1 NMR, and C-13 NMR spectroscopy techniques. Salts and complexes were tested for antiproliferative activities on human breast and prostate cancer cell lines (MCF-7, MDA-MB-23, DU-145) and L-929 normal cells for 24 h, 48 h and 72 h using MTT assays. The Ag(I)-NHC complexes (2a c) showed dose and time-dependent cytotoxic activity against all cell lines. MDA-MB-231 and MCF-7 human breast carcinoma cells were the most sensitive to displaying IC50 lower than 1 mu M at all time points for 2a and 2b complexes respectively. The IC(50)s for Ag(I)-NHC were higher in normal cells especially compared to the breast cancer cells, suggesting that complexes possessed noteworthy selectivity for human breast cancer cells. Complex 2a showed high selectivity (>= 13-fold) for MDA-MB-231 breast cancer cells at all time points. These results also demonstrated that complex 2b has 4-7-fold selectivity against MCF-7 breast cancer cells. (C) 2019 Published by Elsevier B.V.
  • Küçük Resim Yok
    Öğe
    Synthesis, in vitro anticancer activities, and quantum chemical investigations on 1,3-bis-(2-methyl-2-propenyl)benzimidazolium chloride and its Ag(I) complex
    (Sage Publications Ltd, 2021) Serdaroglu, Goncagul; Sahin-Bolukbasi, Serap; Barut-Celepci, Duygu; Sevincek, Resul; Sahin, Neslihan; Gurbuz, Nevin; Ozdemir, Ismail
    1,3-Bis-(2-methyl-2-propenyl)benzimidazolium chloride and its Ag(I) complex are synthesized and the structures are elucidated using spectroscopies techniques. The molecular and crystal structures of the benzimidazolium salt are confirmed by X-ray crystallography. The molecular geometries of the benzimidazolium and its Ag(I) salt are analyzed using the B3LYP functional with the 6-311+G(d,p)/LANL2DZ basis set. The observed Fourier transform infrared and nuclear magnetic resonance isotropic shifts are compared with the calculated values. Besides, the quantum chemical identifiers, significant intramolecular interactions, and molecular electrostatic potential plots are used to show the tendency/site of the chemical reactivity behavior. The three-dimensional Hirshfeld surfaces and the associated two-dimensional fingerprint plots are applied to obtain an insight into the behavior of the interactions in the crystal. Both compounds are tested for their in vitro anticancer activities against DU-145 and MCF-7 cancer cells and L-929 non-cancer cells using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.