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    Assessing Lung Injury Induced by Streptozotocin-induced Diabetes: A Deep Neural Network Analysis of Histopathological and Immunohistochemical Images
    (Bentham Science Publ Ltd, 2025) Senturk, Tugba; Bolat, Demet; Yay, Arzu Hanim; Baran, Munevver; Latifoglu, Fatma
    Introduction Diabetes mellitus is an endocrine disorder characterized by metabolic abnormalities and chronic hyperglycemia, caused by insulin deficiency (Type I) or resistance (Type II). It affects various tissues differently, and its complications extend beyond classical targets, such as the kidneys and eyes, to lesser-studied organs, including the lungs. Understanding tissue-specific damage is crucial for effective disease management and the prevention of complications.Objective This study aims to evaluate the histopathological and immunohistochemical effects of diabetic lung fibrosis using a streptozotocin (STZ)-induced diabetes model. Additionally, it seeks to develop a high-performance image classification system based on deep neural networks to accurately classify tissue damage in diabetic models.Methods Lung tissue samples were collected from the STZ-induced diabetes model and analyzed through histopathological and immunohistochemical techniques. Image data were further processed using convolutional neural networks (CNNs), including pre-trained models, such as ResNet50, VGG16, and SqueezeNet. Classification was conducted in multiple color spaces (RGB, Grayscale, and HSV) and evaluated using performance metrics, including confusion matrix, precision, recall, F1 score, and accuracy.Results The use of color significantly enhanced image patch classification performance. Among the models tested, SqueezeNet in the RGB color space demonstrated the highest accuracy, achieving an F1 score of 93.49% +/- 0.04 and an accuracy of 93.77% +/- 0.04. These results indicated the efficacy of CNN-based classification in detecting lung damage associated with diabetes.Discussion and Conclusion Our findings confirmed that diabetes induces histopathological changes in lung tissue, contributing to fibrosis and potential pulmonary complications. Deep learning-based classification methods, particularly when utilizing color space variations and advanced preprocessing techniques, provide a powerful tool for analyzing diabetic tissue damage and may aid in the development of diagnostic support systems.
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    Histopathological Image Analysis Using Machine Learning to Evaluate Cisplatin and Exosome Effects on Ovarian Tissue in Cancer Patients
    (Mdpi, 2025) Senturk, Tugba; Latifoglu, Fatma; Altintop, Cigdem Guluzar; Yay, Arzu; Gonen, Zeynep Burcin; Onder, Gozde Ozge; Mat, Ozge Cengiz
    Cisplatin, a widely used chemotherapeutic agent, is highly effective in treating various cancers, including ovarian and lung cancers, but it often causes ovarian tissue damage and impairs reproductive health. Exosomes derived from mesenchymal stem cells are believed to possess reparative effects on such damage, as suggested by previous studies. This study aims to evaluate the reparative effects of cisplatin and exosome treatments on ovarian tissue damage through the analysis of histopathological images and machine learning (ML)-based classification techniques. Five experimental groups were examined: Control, cisplatin-treated (Cis), exosome-treated (Exo), exosome-before-cisplatin (ExoCis), and cisplatin-before-exosome (CisExo). A set of 177 Local Binary Pattern (LBP) features were extracted from histopathological images, followed by feature selection using Lasso regression. Classification was performed using ML algorithms, including decision tree (DT), k-nearest neighbors (KNN), support vector machine (SVM), and Artificial Neural Network (ANN). The CisExo group exhibited the most homogeneous texture, suggesting effective tissue recovery, whereas the ExoCis group demonstrated greater heterogeneity, possibly indicating incomplete recovery. KNN and ANN classifiers achieved the highest accuracy, particularly in comparisons between the Control and CisExo groups, reaching an accuracy of 87%. The highest classification accuracy was observed for the Control vs. Cis groups (approximately 91%), reflecting distinct features, whereas the Control vs. Exo groups demonstrated lower accuracy (around 68%) due to feature similarity. Exosome treatments, particularly when administered post-cisplatin, significantly improve ovarian tissue recovery. This study highlights the potential of ML-based classification as a robust tool for evaluating therapeutic outcomes. Additionally, it underscores the promise of exosome therapy in mitigating chemotherapy-induced ovarian damage and preserving reproductive health. Further research is warranted to validate these findings and optimize treatment protocols.