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    Effects of ozone application on fissure sealants prepared different surface etching modalities: An in vitro study
    (2019) Surmelioglu, Derya; Ucar Gundogar, Zubeyde
    Aim: The aim of this study was to compare the microleakage levels of different surface treatment of fissure sealants. Material and Methods: A total of 60 standardized non-erupted third molar teeth were divided into 5 groups (n=12) according to the pre-treatment method; Group 1 (G1): Clearfil Universal Bond Quick (self-etching) + ozone +fissure sealant (FS), Group 2 (G2): Clearfil Universal Bond Quick (self-etching) +FS, Group 3(G3): Clearfil Universal Bond Quick (etch-and-rinse) +ozone +FS, Group 4 (G4): Clearfil Universal Bond Quick (etch-and-rinse) +FS, Group 5 (G5): phosphoric acid etching +FS (control). Following surface pre-treatments, fissures of all teeth were sealed. Then, all specimens were subjected to thermal cycles (5000 times of 5 degrees C/55 degrees C for 1 minute each). All surfaces of the teeth were covered with nail polish twice up to a distance of 1mm to fissure sealant. All teeth were immersed in a 0.5% basic fuchsine dye for 24 hours. To examine microleakage, teeth separated into 2 halves bucco-lingually with saw. Microleakage scores were evaluated under stereomicroscope with a magnification of x40. The scores were statistically analyzed with Kruskal-Wallis and Mann Whitney U tests. Results: G2, G3 and G4 showed statistically significant differences compared to control group, except G1. Although G2 showed highest microleakage level, G4 sowed lowest microleakage level G5 Ozone led to decreased microleakage in images in groups 3, 5 and 6. Conclusions: The fissure sealing ability of with the universal adhesive in etch-and-rinse or self-etch modes was similar to that of conventional acid etching. Ozone pretreatment favorably affected the marginal sealing ability of the tested fissure sealants.
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    Evaluating the performance of led based and lf based devices for the detection of caries under clear and opaque sealants
    (2020) Isman, Ozlem; Surmelioglu, Derya
    Aim: The aim of the present investigation was to evaluate the diagnostic ability of laser fluorescence (LF)-based device and a light emitting diode (LED)-based device regarding the effects of different types of fissure sealants and etching modalities under in vitro conditions.Materials and Methods: All samples (n = 60) were divided into 4 groups. Opaque or clear sealants were applied to the surface of extracted human molar teeth after etching the surface with either acid or laser pretreatment methods. Both the LF-based DIAGNOdent pen device (Kavo, Biberach, Germany) and an LED-based device (Midwest Caries I.D.; Dentsply Professional, York, PA, USA) were used to measure decalcification scores both before and after sealant application and following thermocycling. The Statistical Package for the Social Sciences (SPSS v16.0, IBM, Chicago, IL, USA) was used for statistical analyses. To examine the inter-observer and intra-observer agreements, an intraclass correlation was used. The Wilcoxon test was used to compare the effects of both the sealants and the etching modalities. A p value of 0.05 was accepted as statistically significant. Results: Statistically significant higher readings were achieved with the LED device after applying the opaque sealant in both the acid etched (p = 0.006) and laser treated groups (p = 0.001). The clear sealant did not affect the LF- and LED-based devices’ readings after etching with either acid or the laser. Thermocycling increased the LF readings in the laser etched/clear sealant group, but it did not change the LED-based device’s results.Conclusion: The LF and LED results were not influenced by etching with either acid or a laser on the clear sealant. After the application of an opaque sealant, higher readings on the LED device were achieved for both the laser treated and acid etched groups. Thermocycling did not affect the LED device’s readings.