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    Evaluation of Cytotoxic Effect of Graphene Oxide Added to Mineral Trioxide Aggregate
    (Sage Publications India Pvt Ltd, 2023) Dayi, Burak; Kuecuekyildiz, Elif Nihan; Taghizadehghalehjoughi, Ali
    Aim:Recently, although studies have shown that biomaterials containing graphene oxide (GO) in biomedicine stand out for their positive effects, the effect of GO on dental tissues when used with dental materials is not well known. The aim of this study was an evaluation of the cytotoxic effects of GO on gingival fibroblasts when it is combined in two different ratios with Mineral Trioxide Aggregate (MTA). Materials and Methods:In this in-vitro study, a homogenous mixture of adding +0.1 weight (wt)% and +0.3 wt% GO to Angelus MTA was created (two experimental groups) and compared with pure Angelus MTA and negative control groups. The materials were mixed according to the manufacturer's instructions, and Teflon molds were used to form 24 disc-shaped samples for each group. The samples were divided into groups according to the simple random sampling method. The cytotoxic effect of samples was determined on gingival fibroblast cells by using the MTT test, and total oxidant status (TOS) and total antioxidant capacity (TAC) kits in 24 and 72 hours. The data were statistically analyzed using one-way ANOVA and Tukey tests. Results:A significant difference was found between the material-applied groups and the control group at the TAC 24 and 72 hours and between the groups containing GO and the control group at the MTT 72 hours and TAC and TOS 24 and 72 hours (p < .05). Conclusion:The addition of GO to MTA increased the dose and time-based toxicity and oxidant amount, and decreased antioxidant capacity.
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    Glioblastoma cell-derived exosomes induce cell death and oxidative stress in primary cultures of olfactory neurons. Role of redox stress
    (Springer, 2023) Yeni, Yesim; Taghizadehghalehjoughi, Ali; Genc, Sidika; Hacimuftuoglu, Ahmet; Yildirim, Serkan; Bolat, Ismail
    BackgroundGlioblastoma multiforme, described as glioblastoma, is a malignancy originating from glial progenitors in the central nervous system and is the most malignant subtype of brain tumors which attracted researcher's attention due to their high recurrence and mortality despite optimal treatments. In the study, we aimed to research whether glioblastoma-originated exosomes play a role in olfactory nerve cell toxicity.Methods and resultsFor this aim, exosomes obtained from U373 and T98G cells were applied to olfactory nerve cell culture at distinct doses. Then, glutathione (GSH), lactate dehydrogenase (LDH), total antioxidant capacity (TAC), 3-(4,5-Dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide (MTT), total oxidant status (TOS) and Immunofluorescence analyzes were performed. We found that both glioblastoma-derived exosomes decreased cell viability in olfactory neurons with increasing doses. According to the obtained data, the olfactory neuron vitality rate was 71% in T98G-exosome, but the decrease in U373-exosome was more obvious (48%). In particular, the 100 mu g/ml dose exacerbated oxidative stress by increasing TOS. It also increased cellular apoptosis compared to the control group due to LDH leakage. However, the results of GSH and TAS showed that antioxidant levels were significantly reduced.ConclusionIn the microenvironment of olfactory neurons, GBM-derived exosomes increased oxidative stress-induced toxicity by reducing TAC and GSH levels. Therefore, glioblastoma cells by induction of exosome-based stress support malignant growth.