DSpace Arşivi :: by Yazar "Tanriverdi, Elif Seren" değerine göre listeleniyor

Yazar "Tanriverdi, Elif Seren" seçeneğine göre listele

Listeleniyor 1 - 20 / 60

A first study of meat-borne enterococci from butcher shops: prevalence, virulence characteristics, antibiotic resistance and clonal relationship
(Springer, 2024) Cebeci, Tugba; Tanriverdi, Elif Seren; Otlu, Baris
IntroductionEnterococcus, which used to be thought of as a harmless commensal living in the digestive tract, has now become a highly resistant and highly contagious pathogen that makes nosocomial infections much more common. This study examined enterococci species and their antibiotic resistance phenotypes and genotypes and virulence gene content in Turkish ground beef samples. Methodology A total of 100 ground beef samples were analyzed between May 2020 and May 2021. The isolated strains were identified via matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and confirmed using polymerase chain reaction (PCR) after which they were divided into several species using PCR and tested for antibiotic resistance against 19 antimicrobial agents using the disc diffusion method. The genetic similarity analysis, pulsed-field gel electrophoresis (PFGE) was performed. Results A total of 93 isolates in ground beef were identified, comprised of E. faecalis 72.04%; E. hirae- 11.82%; E. casseliflavous- 7.52%; E. faecium- 5.3%; E. gallinarium- 3.23%. The virulence genes observed in Enterococcus species were distributed as follows: gelE 88.1%, ace 53.7%, efaA 40.8%, asaI 19.3%, esp 6.4%, and cylA 1.07%. A high antibiotic resistance was recorded for tetracycline (43.01%), followed by ampicilin (17.2%), and chloramphenicol (13.9%). 17.2% of Enterococcus isolates were multidrug-resistant. The study determined the high prevalence of antibiotic resistance genes, specifically for tet(L) 10 (10.7%), aac(6')Ie-aph(2)-la 3 (3.2%), and ermB 3 (3.2%). The presence of efflux pump genes were identified in 74.1% of Enterococcus isolates. Genetic characterization of 67 E. faecalis isolates by PFGE revealed 41 pulsed-field gel electrophoresis (PFGE) patterns that were grouped into 15 clusters, which presented more than one strain with 100% similarity. Conclusion Isolates obtained from several areas and butchers had comparable patterns of PFGE, suggesting that the presence of circulating E. faecalis poses a potential public health concern in diverse districts. To mitigate the health hazards associated with the contamination of enterococci from raw to cooked meats, it is necessary to enhance the disinfection of butcheries, promote excellent hand hygiene among butchers, and implement appropriate meat storage and handling methods to prevent bacterial development.
A widespread dissemination of Bacillus licheniformis in a tertiary hospital: an outbreak or pseudo-outbreak?
(Cambridge Univ Press, 2025) Deniz, Secil; Caliskan, Ahmet; Tanriverdi, Elif Seren; Ozkan, Burhan; Karahan, Zeynep Ceren; Kacar, Ilknur; Kivrak, Ayse
Objective: Bacillus licheniformis is a Gram-positive bacterium commonly found in soil and water. There have been very few reports on the pathogenicity of B. licheniformis in humans. In this prospective study, the emergence of cases affected by B. licheniformis during a period of 38 days was reported together with investigations into the sources of spread to hospitalized patients in a tertiary hospital.Methods: Blood cultures of 45 patients grew Bacillus spp. in October and November, 2021. To identify the source and prevent further dissemination of the pathogen, all commonly used materials were examined. Samples obtained from alcohol/water solutions yielded positive results for Bacillus spp., which pointed to the main distilled water tank of the hospital, subsequently found to be the main source. All isolates were sent for molecular analysis by arbitrarily-primed polymerase chain reaction (AP-PCR).Results: Molecular analysis with AP-PCR of 29 positive cultures showed a closely related clone of B. licheniformis in 25 specimens, including 23 blood samples and two distilled water samples. Considering the rarity of true infections with B. licheniformis and the mild clinical picture of the affected patients, the dissemination was considered to be a pseudo-outbreak.Conclusions: Prompt detection and elimination of any pathogenic spread and differentiation of a pseudo-outbreak from a true outbreak are of utmost importance in preventing unnecessary antibiotic prescriptions, diagnostic procedures, and interventions.
An Intensive Care Outbreak Caused by Burkholderia cepacia from Bacterial Filters
(Mdpi, 2025) Aytac, Ozlem; Tanriverdi, Elif Seren; Gundag, Omur; Senol, Feray Ferda; Karlidag, Gulden Eser; Otlu, Baris
Background: We report a hospital outbreak caused by Burkholderia cepacia that occurred in 16 patients admitted to intensive care units in Elaz & imath;& gbreve;, T & uuml;rkiye, between 19 March and 23 April 2024. Methods: The outbreak investigation was initiated on 23 March 2024, four days after B. cepacia was detected in four different patients. Environmental samples were collected from various parts of the hospital to find the source of the outbreak. Arbitrarily Primed Polymerase Chain Reaction (AP-PCR) was performed to determine the genetic relationship between environmental and patient samples. Results: In total, 16 of 18 B. cepacia isolates were obtained from tracheal aspirate culture. A total of 10 of 16 patients developed hospital-acquired pneumonia due to B. cepacia. Among the environmental cultures in the intensive care units, only the respirator bacterial filter grew. The isolate obtained here was in the same cluster as the isolate obtained from patient samples, resulting in a dominant clustering rate of 94.4%. Conclusions: Improper and inappropriate use of respirators and equipment can lead to outbreaks. Early detection of the outbreak, identification of the source, and taking appropriate measures quickly to contain the outbreak are key.
Bacteriological profile of diabetic foot ulcers and analysis of serum meteorin levels
(Springer, 2025) Erdogan, Esra; Yetisgen, Azize; Keskin, Lezzan; Tanriverdi, Elif Seren; Ugur, Isilay Gokce Benk
ObjectiveWith the increase in the incidence of diabetes mellitus (DM) in recent years, diabetic foot ulcers (DFU), which are common and serious chronic complications of diabetes, have also become widespread. DFU is highly associated with a significant deterioration in quality of life as well as increased morbidity and mortality. Meteorin is a potent neurotrophic growth factor and shows antiangiogenic, antihyperalgesic, antinociceptive and neuroprotective effects. This study aimed to determine the possible relationship between meteorin, diabetes and diabetic foot ulcer by comparing the serum meteorin levels of healthy control group, DM patients and patients with diabetic foot ulcers.MethodsOur study included a total of 62 diabetic patients, 31 of whom had DFU, and 29 healthy individuals as a control group. Meteorin levels of the participants were measured using ELISA method in serum samples. Other laboratory and epidemiological data of the patients were obtained from the hospital database.ResultsIn the wound cultures taken from patients with DFU, the most commonly isolated bacteria were Staphylococcus aureus and Pseudomonas aeruginosa. Serum meteorin levels were found to be statistically significantly higher in diabetic patients as compared to the healthy control group, and among diabetic patients, those with DFU had significantly higher levels compared to those without DFU. A positive significant correlation was found between meteorin level and age, HbA1c, WBC, urea, sedimentation, CRP and ferritin.ConclusionThe results of our study, aimed at better understanding the biological functions and potential clinical applications of meteorin, suggest that meteorin could potentially be used as a biomarker for the development of DFU.
Bir Üniversite Hastanesi’nde 2018-2019 Yıllarında İzole Edilen Staphylococcus Aureus İzolatlarının İncelenmesi
(2020) Tanriverdi, Elif Seren; Duman, Yucel; Tekerekoglu, Mehmet
Amaç: Staphylococcus aureus toplum ve sağlık bakım ilişkili cilt ve yumuşak doku enfeksiyonları, üriner sistem enfeksiyonu, endokardit, pnömoni,septik artrit, osteomyelit, sepsis gibi morbidite ve mortalitesi yüksek enfeksiyonlara neden olan en önemli mikroorganizmalardan biridir. Çalışmamızda hastanemiz Tıbbi Mikrobiyoloji laboratuvarına gelen çeşitli klinik örneklerden izole ettiğimiz metisiline dirençli S. aureus (MRSA) ve metisilineduyarlı S. aureus (MSSA) izolatlarının antimikrobiyallere direnç oranlarının belirlenmesi ve hastanemize ait direnç profilinin incelenmesi amaçlandı.Gereç ve Yöntem: 2018- 2019 yılları arasında izole edilen 879 S. aureus izolatı çalışmaya dahil edildi. İzolatlar konvensiyonel yöntemlerle veMALDI-TOF MS (Bio Merieux, Fransa) ile identifiye edildi. İzolatların antimikrobiyal duyarlılık testleri European Committee on AntimicrobialSusceptibility Testing (EUCAST) kriterlerine göre Kirby-Bauer disk düffüzyon ve Gradient düffüzyon methodu ile belirlendi.Bulgular: 2018 yılında %24.2, 2019 yılında ise %22.3 olmak üzere iki yıllık sürede MRSA oranı %23.3 olarak belirlendi. S. aureus izolatlarınındağılımına bakıldığında yara kültürlerinin en sık S. aureus izole edilen klinik örnek olduğu tespit edildi. MRSA ve MSSA izolatlarında vankomisin velinezolide direnç belirlenmedi. En yüksek direnç oranı MRSA ve MSSA izolatlarında sırasıyla %98 ve %82.8 ile penisiline karşı tespit edildi.Sonuç: Hastanemizdeki S. aureus izolatlarının direnç profillerinin bilinmesi; ampirik tedavide yol gösterici olacaktır. Ayrıca etkili enfeksiyon kontrolönlemlerinin alınması, el hijyenine dikkat edilmesi ve kısıtlı antibiyogram uygulaması MRSA enfeksiyonlarının kontrolüne katkı sağlayacaktır.
Carbapenem-resistant Klebsiella pneumoniae outbreak with monoclonal spread: Evaluation of resistance genes and ceftazidime-avibactam susceptibility
(Elsevier, 2023) Arici, Neslihan; Tanriverdi, Elif Seren; Calik, Seyma; Kansak, Nilgun; Adaleti, Riza; Otlu, Baris; Aksaray, Sebahat
Purpose: The aim of this study was to investigate ceftazidime-avibactam (CAZ-AVI) susceptibility, carbapenemase genes, and clonal relationship in carbapenem-resistant Klebsiella pneumoniae (CrKp) isolates.Methods: A total of 28 non-repetitive CrKp isolates with positive carbapenemase production determined by the modified carbapenem inactivation method (mCIM), were included in the study. Identification of the isolates was performed with MALDI-TOF MS (VITEK-MS, bioMerieux, France). The automated system (VITEK-2, bioMerieux) and gradient diffusion test (Etest, bioMerieux) were used to determine antibiotic susceptibility. The mCIM was performed according to CLSI (2021) recommendations. CAZ-AVI susceptibility was carried out using the standard disc diffusion method. Results were evaluated according to EUCAST 2022 criteria. The blaOXA-48, blaNDM, blaKPC, blaIMP and blaVIM genes were investigated by multiplex PCR. The clonal relationship between isolates was determined by both AP-PCR and PFGE methods.Results: Of the total 28 isolates, 89.3% were susceptible to CAZ-AVI. blaOXA-48 gene was found in 85.7% of the isolates, blaOXA-48 thorn blaNDM gene in 10.7%, and blaNDM gene in 3.6%. blaKPC, blaIMP and blaVIM genes were not detected. Three clusters with three different genotypes were determined by the PFGE method. The largest cluster was Genotype A (n:24), followed by Genotype B (n:3), and Genotype C (n:1). AP-PCR was highly compatible with PFGE. The isolates of Genotype A, mostly from the intensive care unit (ICU), were evaluated as outbreak strains with monoclonal dissemination. Conclusions: OXA-48 remains the most frequently detected enzyme in CrKp strains in our country. The ceftazidime-avibactam susceptibility rate of 89.3% indicates that this antibiotic is still effective against CrKp isolates. The unnoticed outbreak detected in our study revealed the severity of intra-hospital cross-contamination affecting different wards, including the ICU. Therefore, in order to limit the spread of CrKp isolates, it is of great importance to implement strict infection control measures, and molecular surveillance programs, especially in the ICU.
A Case of Infective Endocarditis Due to Abiotrophia defectiva, and Treatment with Ertapenem
(Galenos Publ House, 2021) Duman, Yucel; Altunisik Toplu, Sibel; Tanriverdi, Elif Seren; Bayindir, Yasar
The most frequently reported infections due to Abiotrophia defectiva worldwide are bacteraemia and endocarditis. It is estimated that A. defectiva causes approximately 5-6% of microbiologically proven endocarditis and plays a role in the etiology of culture negative endocarditis. In this article we reported a patient with infective endocarditis (IE) due to penicillin resistant A defectiva, which caused vegetation in the mitral valve, embolism in the spleen and successfully treated with ertapenem. A 70-year-old female patient was admitted to the emergency service with fever and abdominal pain. The patient had a pronounced widespread abdominal tenderness in the left quadrant. The body temperature was 38.9 degrees C, C-reactive protein (CRP) level was 12.7 mg/dl and white blood cell count was 13.3x10(3) mm(3). The patient was hospitalized to investigate fever's reason. It was learned in the anamnesis that she used warfarin. Considering the pre-diagnosis of urinary tract infection, empirical treatment with ceftriaxone 2x1 g/day was started. However, due to the increase in International Normalized Ratio value on the second day of ceftriaxone treatment, ertapenem 1x1 g/day was started by considering drug interaction between warfarin and ceftriaxone. Transesophageal echocardiography of the patient revealed a 1.2x1.2 cm vegetation on the posterior leaflet surface of the mitral valve, and ischemia due to embolism in the spleen was detected on abdominal computer tomography. Penicillin resistant A defectiva grew in the blood culture. The patient was diagnosed as having IE on the seventh day of her admission. Ertapenem treatment was completed in six weeks due to the patient's absence of fever, decreased CRP level, and the absence of A. defectiva in the seventh day control blood culture. After treatment, vegetation was not observed in the transthoracic echocardiography of the patient and no signs of ischemia was detected in the spleen. Detection of A. defectiva shaped the follow-up process of our patient by bringing the diagnosis of IE to mind. In treatment of A defectiva-related IE, guidelines suggest long-term combination of aminoglycosides with beta-lactams or vancomycin. However, in our patient, treatment was provided by using ertapenem alone for six weeks. In addition, we believed that early diagnosis and treatment in our patient prevented serious complications.
A Case of Intra-abdominal Tuberculosis Due to Mycobacterium bovis Mimicking Ovarian Cancer: Importance of Microbiological Diagnosis
(Ankara Microbiology Soc, 2022) Dalgic, Bahise Cagla Taskin; Yenisehirli, Gulgun; Tanriverdi, Elif Seren; Akkan, Murat; Otlu, Baris; Sezer, Oznur; Koseoglu, Handan inonu
Mycobacterium bovis causes gastrointestinal tuberculosis by being transmitted through consumption of infected milk and dairy products, mostly in developing countries, and can spread to the other neighbourhood intra-abdominal tissues and organs. In addition to the symptoms such as weight loss, weakness, abdominal pain, and chronic diarrhea in female patients with abdominal tuberculosis, findings such as pelvic mass, ascites and CA-125 elevation may be encountered. Patients with these symptoms usually preliminary diagnosed as having ovarian cancer. It is very important to distinguish between these two diseases quickly, which have different treatment protocols. In this case report, a case of intra-abdominal tuberculosis caused by M.bovis, whose diagnosis was confirmed by microbiological methods with the findings mimicking ovarian cancer such as weight loss, ascites, pelvic mass and increased CA-125 was presented. Tuberculosis was considered in the differential diagnosis of a 23-year old female patient with abdominal pain, weight loss, ascites, pelvic mass, and elevated CA-125 (643.9 U/ml) findings and a mass in the left tubaovarian region on abdominal CT. The ileum biopsy sample taken during colonoscopy and ascitic fluid sample taken with paracentesis were sent to our laboratory for acid-fast bacilli (AFB) staining and tuberculosis culture. In our laboratory, samples were incubated in both liquid culture system [BACTEC MGIT 320 Mycobacteria Culture System (Becton Dickinson,USA)] and solid culture medium [Lowenstein-Jensen Medium (Becton Dickinson,USA)] and AFB smears were performed. While AFB smears were negative, ileum biopsy material showed growth on day 14 and ascitic fluid sample on day 11 in liquid culture medium. AFB smear was prepared from broth and red bacilli were seen on a blue background that formed cord factor. The bacillus was identified as Mycobacterium tuberculosis complex by the immunochromatographic rapid test [BD MGIT TBc Identification Test (BD,USA)]. The anti-tuberculosis drug treatment was initiated with the diagnosis of intra-abdominal tuberculosis. The isolated bacillus was found to be sensitive to isoniazid, rifampicin, ethambutol and resistant to streptomycin, according the drug susceptibility test results. Subspecies identification of M.tuberculosis complex was investigated by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) but could not be determined by this method. Genotyping was performed with the GenoType MTBC VER 1.X (Hain Lifescience, HardwiesenstraBe, Germany) kit. The isolate was identified as M.bovis. In the follow-up of the patient three months later, it was determined that tumor markers, ascitic fluid and intra-abdominal lymph nodes regressed significantly and the mass in the left ovary completely disappeared. In this report, we presented a case with intra-abdominal tuberculosis whose clinical, radiological and laboratory findings mimic ovarian cancer to imply the importance of microbiological diagnosis.
Ceftazidime-avibactam resistance determination in carbapenem-resistant Klebsiella pneumoniae infections before its use in practice
(J Infection Developing Countries, 2024) Eren-Kutsoylu, Oya Ozlem; Ceylan-Cimendag, Hacer; Sari-Kaygisiz, Ayse Nur; Tanriverdi, Elif Seren; Ozbek, Ozgen Alpay; Oktem, Ibrahim Mehmet Ali; Otlu, Baris
Introduction: To ensure the appropriate usage of ceftazidime-avibactam (CAZ-AVI), recently introduced in our hospital, we aimed to determine susceptibility rates, enzyme analysis, and clonal relationship among strains, together with clinical data. Methodology: Between June 1 and September 30, 2021, demographic and microbiological data of the patients were recorded. In the obtained samples, meropenem and colistin minimal inhibitory concentration (MIC) levels, carbapenem resistance genes, and the clonal relationship were studied by molecular methods. CAZ-AVI was not used in any of the patients. Results: 140 carbapenem-resistant Klebsiella pneumoniae were isolated from 57 patients. Resistance to CAZ-AVI was found in 76 (54.3%) strains. Out of 57 patients, 31 (54.4%) isolates could be reached. Meropenem MIC level was >= 32 mu g/mL in 26 (83.9%), and colistin MIC level was >= 4 mu g/mL in 17 (54.8%) isolates. Enzyme analysis revealed NDM in 20 (64.5%), OXA-48 in 17 (54.8%), and KPC in seven (22.6%). NDM + OXA-48 was determined in 10 (32.2%) strains. NDM was determined in all CAZ-AVI resistant strains, OXA-48 in 16.1% (2/5) strains. Seven genotypes were detected. The largest cluster was genotype 3 clusters (11 isolates). Of 31 patients, 22 (71.0%) died. CAZ-AVI was susceptible in one of the patients who survived and four who died. Conclusion: Before using a new antibiotic, each center should determine the basal data and phenotypic/genotypic resistance ratios specific to that antibiotic. While a high NDM rate and low CAZ-AVI sensitivity limit the use of the drug in our center, it is clear that CAZ-AVI use in sensitive strains will decrease mortality.
Cessation of Rectal Screening for Vancomycin-Resistant Enterococci: Experience from a Tertiary Care Hospital from Turkiye
(Mdpi, 2023) Dizman, Guelcin Telli; Metan, Goekhan; Zarakolu, Pinar; Tanriverdi, Elif Seren; Hazirolan, Guelsen; Aytac Ak, Hanife; Kilincarslan, Dilek
Objective:Here, we compared the impact of different polices on the epidemiology of Vancomycin-resistant Enterococcus faecium bloodstream infections (VRE-BSIs) in a tertiary care hospital including two hospital buildings (oncology and adult hospitals) in the same campus. Material and Methods:All patients who were hospitalized in high-risk units were screened weekly for VRE colonization via rectal swab between January 2006 and January 2013. After January 2013, VRE screening was only performed in cases of suspicion of VRE outbreak and during point prevalence studies to evaluate the epidemiology of VRE colonization. Contact precautions were in place for all VRE-positive patients. The incidence density rates of hospital-acquired (HA)-VRE-BSIs were compared between two periods. Results:While the rate of VRE colonization was higher in the second period (5% vs. 9.5% (p < 0.01) for the adult hospital, and 6.4% vs. 12% (p = 0.02 for the oncology hospital), there was no increase in the incidence rate HA-VRE BSIs after the cessation of routine rectal screening in either of the hospitals. Conclusion:Screening policies should be dynamic and individualized according to the epidemiology of VRE as well as the workforce and cost. Periodical rectal screening of VRE can be discontinued if suspicion of an outbreak can be carefully monitored.
Chemical composition, antimicrobial activities, and molecular docking studies of Turkish propolis ethanol extract
(Czech Academy Agricultural Sciences, 2023) Ozbey, Gokben; Muz, Mustafa Necati; Tanriverdi, Elif Seren; Erkan, Sultan; Bulut, Niyazi; Otlu, Baris; Zigo, Frantisek
The purpose of the present study was to investigate the antimicrobial effect of propolis ethanol extract col-lected from the Tarsus district of Mersin province, Kilis province, Yayladagi district of Hatay province in southern Turkiye and Sarkoy district of Tekirdag province of northwestern Turkiye against Escherichia coli (ATCC 25922), Helicobacter pylori (ATCC 43504), Pseudomonas aeruginosa (ATCC 27853), and Staphylococcus aureus (ATCC 29213). Their chemical constituents were detected via liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). They were used in a molecular docking approach to search the interactions between the propolis compounds. A total of 24 phenolic compounds were detected in all samples. 3-4 dimethoxycinnamic acid, caffeic acid and genistein were indicated to be the predominant phenolic compounds in propolis extracts by LC-MS/MS, while rutin was found in the lowest concentra-tion. Phenolic compounds were detected in a high concentration of the propolis samples collected from the Tarsus dis-trict of Mersin province. The broth microdilution method determined minimum inhibition concentration (MIC) values. MIC values ranged from 0.02 to 14 mg center dot mL-1. E. coli and S. aureus examined were as susceptible to the propolis extracts ex-cept for Mersin and Tekirdag propolis samples. The propolis sample collected from the Tarsus district of Mersin province presented the highest antibacterial activity on P aeruginosa with MIC values of 1 mg center dot mL-1. Active substances in propolis were docked to the relevant target proteins (5LMM, 4NX9, 5YHG, and 5FXT) representing E. coli (ATCC 25922), H. py-lori (ATCC 43504), P aeruginosa (ATCC 27853), and S. aureus (ATCC 29213), and with the help of molecular simulation. With this study, we indicated that the ethanol extract of propolis had a stronger antibacterial activity on S. aureus isolates than that of E. coli, H. pylori, and P aeruginosa. Although each component of propolis contributed to the antibacterial activity, the contribution of the vitexin component to the antibacterial activity was found to be quite significant.
Clinical Properties of COVID-19 Developed in the Patients with Tuberculosis
(Ankara Microbiology Soc, 2022) Otlu, Baris; Yakupogullari, Yusuf; Tanriverdi, Elif Seren; Bentli, Recep
There are limited publications about the Coronavirus disease 19 (COVID-19) clinic developing in the patients with active tuberculosis (TB). In this study, it was aimed to determine some clinical features of patients diagnosed with TB who also had COVID-19. In this retrospective cross-sectional study, 71 pa- tients with COVID-19 were evaluated out of a total of 595 patients diagnosed with TB in our province between 2015 and 2021. After contracting COVID-19, a total of nine (12.6%) TB patients were hospital- ized, five (7%) patients were admitted to the intensive care unit, three (4.2%) were intubated, and one (1.4%) died due to severe COVID-19. The frequency of such health problems was found to be higher than the normal population living in the same province. None of these complications were observed in a total of 40 female TB patients, and the hospital and intensive care unit admission rates for men were significantly higher than for women. The results of this study showed that men with active TB had more health problems due to COVID-19 than the normal population. Comprehensive studies are needed to detail the resilience of female TB patients against COVID-19.
Comparison of ceftazidime-avibactam with other appropriate antimicrobial therapy for the treatment of OXA-48-or KPC-producing Enterobacterales infections in Türkiye: A multi-centre retrospective matched-cohort study
(Elsevier, 2026) Aslan, Abdullah Tarik; Tanriverdi, Elif Seren; Kaya, Sibel Yildiz; Dalgan, Gozde; Saltoglu, Nese; Yilmaz, Ezgi; Cicek, Yeliz
Objective: Due to underrepresentation of carbapenemase-producing Enterobacterales infections in randomized controlled trials with ceftazidime-avibactam (CZA) and high cost of CZA therapy, other appropriate antimicrobial therapies (OAAT) are still being used for OXA-48- or KPC-producing Enterobacterales infections in T & uuml;rkiye. Methods: We conducted a multicentre retrospective 1:1 matched cohort study of patients who received >= 48 h of CZA or OAAT for documented OXA-48- or KPC-producing Enterobacterales infections. Patients were matched based on (1) the number of days (+/- 1 d) from the infection onset to the initiation of therapy, (2) INCREMENT-CPE score (+/- 1), (3) source of infection, (4) year of infectious episode, and (5) type of causative microorganism. Results: From 5 Turkish university hospitals, 180 patients were enrolled. Baseline characteristics were all similar between treatment groups. At the time of treatment initiation, 63.9% of patients were in the intensive care unit, 35.6% had septic shock and 41.1% required mechanical ventilation support. Thirty-day mortality occurred in 35.6% (32/90) of patients treated with CZA and in 56.7% (51/90) of those receiving OAAT regimens (P = 0.004). Twenty-one-day clinical response was seen in 50% (45/90) and 26.7% (24/90) of patients receiving CZA and OAAT, respectively (P = 0.002). In multivariable logistic regression analyses, CZA treatment was associated with less likelihood of mortality (aOR = 0.37; 95% CI: 0.19-0.71; P = 0.003) and higher likelihood of 21-d clinical response (aOR = 3.32; 95% CI: 1.68-6.53; P < 0001). Conclusions: Treatment with CZA is associated with more favourable clinical outcomes in treatment of OXA-48- or KPC-producing Enterobacterales infections. A randomized controlled trial is needed to confirm these results. (c) 2025 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)
Comparison of Rotavirus Genotypes Before and During the COVID-19 Pandemic
(Galenos Publ House, 2024) Alici, Ayse; Tanriverdi, Elif Seren; Budak, Ilayda; Akkan, Murat; Yenisehirli, Gulgun; Otlu, Baris
Introduction: Rotaviruses are the leading cause of severe diarrhea in infants and young children worldwide. To date, 32 distinct G genotypes and 47 distinct P genotypes have been identified in group A rotaviruses. Following the Coronavirus disease-2019 (COVID-19) pandemic, our country implemented several measures that effectively reduced the incidence of infectious diseases, including acute gastroenteritis associated with COVID-19. In this study, we investigate whether the measures implemented following the COVID-19 pandemic led to changes in the rotavirus genotype distribution. Materials and Methods: A total of 128 stool samples that tested positive for rotavirus antigen- 64 from the pre-pandemic period and 64 from the pandemic period- were further analyzed for genotyping. As determined by reverse transcription-polymerase chain reaction, rotavirus RNA was detected in 50 (78%) samples from the pre-pandemic period and 51 (80%) samples from the pandemic period. Results: In the pre-pandemic period, the following results were observed among the patients studied by us: G9P[8] in 24 (48%), G1P[8] in 14 (28%), G2P[8] in five (10%), G2P[4] in three (6%), G3P[8] in two (4%), G4P[8] in one (2%), and G9P[4] in one (2%). During the pandemic period, the following results were observed in the patients studied by us: G9P[8] in 28 (54%), G1P[8] in 12 (24%), G2P[8] in six (12%), G2P[4] in two (4%), G3P[8] in one (2%), G4P[8] in one (2%), and G9P[4] in one (2%). Conclusion: In our study, G9P[8] was the dominant genotype during both periods, showing no significant difference in rotavirus genotypes between the pre-pandemic and pandemic periods.
Detection of SARS-CoV-2 RNA in the Serum Samples of Healthy Blood Donors
(Galenos Publ House, 2023) Dalgic, Bahise Cagla Taskin; Yenisehirli, Gulgun; Tanriverdi, Elif Seren; Alici, Ayse; Otlu, Baris
Introduction: The transmission rate of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by blood transfusion is thought to be low; however, it has not yet been proven whether the virus is transmitted by blood transfusion. Published studies have reported that SARS-CoV-2 RNA has been detected in the blood, plasma, or serum of infected individuals. This study aimed to investigate the presence of SARS-CoV-2 RNA in the serum of blood donors and evaluate the risk of transmission of SARS-CoV-2 by transfusion. Materials and Methods: In this study, 200 blood samples were taken from volunteer blood donors. In all serum samples, SARS-CoV-2 RNA was detected by reverse-transcription quantitative polymerase chain reaction assay. Medical records of the donors and recipients were retrospectively reviewed. Results: SARS-CoV-2 RNA was detected in seven (3.5%) of the donor serum samples. None of the positive donors had symptoms of coronavirus disease 2019 (COVID-19), and none had been admitted to the hospital after donation. Seven SARS-CoV-2 RNA-positive donor blood components were given to 12 recipients. No medical records indicated that COVID-19 occurred after the transfusion of blood components for recipients. Conclusion: This study demonstrated the presence of SARS-CoV-2 RNA in the serum of asymptomatic donors. Although our data suggest that the transfusion of blood products from asymptomatic donors to recipients with SARS-CoV-2 RNA in their serum may not result in COVID-19, further studies are needed to prove that SARS-CoV-2 is not transmitted by blood transfusion.
Determination of Gene Mutations Associated with Macrolide and Fluoroquinolone Resistance in Patients Infected with Mycoplasma genitalium
(Ankara Microbiology Soc, 2024) Caliskan, Zeynep Cansu; Tanriverdi, Elif Seren; Uzun, Mertcan; Otlu, Baris; Zarakolu, Pinar
A sexually transmitted bacterium, Mycoplasma genitalium has varying rates of reported resistance to macrolide and some fluoroquinolone group antimicrobials recommended for the treatment of its infections. It is currently recommended that the treatment of these must be planned according to macrolide resistance status. The aim of this study was to determine the presence of macrolide resistance associated mutations (MRM) and fluoroquinolone resistance associated mutations (QRM) in patients infected with M.genitalium. Sixty-one patients who were >= 18 years old, presented to our outpatient clinic between March 2017-March 2022, had symptoms of urethritis/cervicitis according to the Centers for Disease Control and Prevention definition were included in the study. By nucleic acid amplification test (NAAT), the presence of M.genitalium (Mycoplasma-Ureaplasma-OSR for BD MAX, BioGX, the Netherlands) as well as Neisseria gonorrhoeae, Chlamydia trachomatis and Trichomonas vaginalis (BD- MAX system, BD Diagnostics, USA) in the first stream urine samples was determined. Patients' age, gender, sexual orientation if indicated, diagnostic test results for human immunodeficiency virus (HIV) and syphilis, history of antibiotic use in the last three months, presence of concomitant microorganisms detected by NAAT and urine culture results of the symptomatic period were also recorded. Urine samples in which M.genitalium was detected were stored at-80 degrees C until the study day. On the study day, they were thawed and a modified real-time polymerase chain reaction (Rt-PCR) test was performed targeting the V region (147 bp) of the 23S rRNA gene for MRM and gyrA (nucleotides 172-402), gyrB (nucleotides 1256-1480), parC (nucleotides 164-483) and parE (nucleotides 1210-1489) gene regions for QRM. IBM SPSS 25 (IBM Inc., Armonk, NY, USA) software was used for descriptive statistical analysis of the patient data. Of the patients; 49 were male, 12 were female. The age range was 20-57 years. Sexual orientation of 15 (30.6%) male patients was men who have sex with men (MSM). Sixteen (26.2%) were individuals living with HIV and 14 (87.5%) were MSM. Four patients had previous syphilis infection. By NAAT, a second microorganism was present in 30 patients with M.genitalium; Ureaplasma urealyticum in 27 (90%), C.trachomatis in two (6.7%) and N.gonorrhoeae in one (3.3%) patient. Urine cultures performed in 42 (68.8%) of 61 patients during the symptomatic period yielded Lactobacillus delbrueckii in one patient. Eighteen (29.5%) patients had a history of antimicrobial use in the last three months. Macrolide resistance associated mutations was detected in 45 (73.8%) and QRM in 20 (32.8%) of M.genitalium infected individuals. Of those with MRM, 17 (37.8%) had concurrent QRM. Macrolide resistance associated mutations were detected at positions A2071G (75.6%) and A2072G (24.4%) in the 23S rRNA gene. The presence of QRM was detected in parC (85%) and gyrA regions (15%). C234T mutation in parC was detected in nine patients (45%), C184T in four, A248T in three and A248A in one, whileA288G mutation in gyrA was detected in two patients and G285T mutation in one. Chi-square test showed no significant correlation between the presence of mutations associated with resistance and MSM, HIV/syphilis infection status and antimicrobial use in the last three months (p> 0.05). To the best of our knowledge, our study is the first study on antimicrobial resistance of M.genitalium in T & uuml;rkiye and emphasizes the importance of macrolide resistance in symptomatic patients infected with M. genitalium. Further studies are needed for the clinical significance of mutations associated with fluoroquinolone resistance. Determination of antimicrobial resistance in M.genitalium diagnostic tests will be useful in terms of guiding treatment and preventing inappropriate treatment approaches in the early period.
Determination of safety status and probiotic properties of Enterococcus strains isolated from traditional cheeses in Turkey
(Oxford Univ Press, 2023) Kanak, Eda Kilic; Yilmaz, Suzan Ozturk; Altindis, Mustafa; Tanriverdi, Elif Seren; Otlu, Baris
Aims This study aimed to evaluate the probiotic properties of Enterococcus strains isolated from Turkish traditional cheeses. Methods and results Fifty-two Enterococcus spp. were taxonomically determined as follows: Enterococcus faecium (26), Enterococcus faecalis (18), Enterococcus durans (6), and Enterococcus italicus (2). The ability of isolates/strains to survive the harsh conditions (acidity and in-vitro gastric solution) of the gastrointestinal tract was established. They also showed auto-aggregation, hydrophobicity, and co-aggregation ability. Hydrophobicities of the strains were found between 0.8%-21%, 0.7%-56%, and 2%-63% for xylene, chloroform, and ethyl acetate, respectively. Autoaggregation values of the Enterococcus strains were 4%-20%, 7%-30%, and 36%-98% after 2, 4, and 24-h incubation, respectively. In this study, the Enterococcus strains tested showed co-aggregation ability with the Escherichia coli ATCC 25922, Salmonella Typhimurium ATCC 14028, and Staphylococcus aureus ATCC 25923. The results of PCR amplification revealed that only five strains possess virulence factor genes (gelE,asa1,cyl A,esp). We determined antibiotic resistance, biofilm forming abilities, and hemolytic activity for safety evaluation of strains. Conclusions In this large and comprehensive study, we found that only few of Enterococcus strains have promising probiotic potential, among which E. faecalis ES1 and E. faecium EM1 showed the best probiotic properties (are the most promising probiotic candidates).
Does marking as sterile mean really sterile? Stenotrophomonas maltophilia outbreak caused by a blood-gas injector containing liquid heparin
(Cambridge Univ Press, 2023) Dizman, Guelcin Telli; Metan, Gokhan; Karahan, Gizem; Tanriverdi, Elif Seren; Hazirolan, Gulsen; Otlu, Baris; Sonmezer, Meliha Cagla
An outbreak investigation was initiated after detecting an increase in the number of patients with Stenotrophomonas maltophilia bloodstream infections (SM-BSIs) througout the hospital. S. maltophilia was isolated from the cultures of blood-gas injectors containing liquid heparin. The incidence density of SM-BSIs decreased significantly after prohibiting the use of those injectors.
The Effects of Antiperspirant Aluminum Chlorohydrate on the Development of Antibiotic Resistance in Staphylococcus epidermidis
(Mdpi, 2023) Aras, Ayse; Rizvanoglu, Suna Sibel; Tanriverdi, Elif Seren; Karaca, Basar; Eryilmaz, Mujde
This study investigates the effects of the antiperspirant aluminum chlorohydrate on the development of antibiotic resistance in commensal Staphylococcus epidermidis isolates. The isolates were exposed to aluminum chlorohydrate for 30 days. The bacteria that developed resistance to oxacillin and ciprofloxacin were isolated, and the expression levels of some antibiotic resistance genes were determined using quantitative reverse transcriptase PCR. Before and after exposure, the minimum inhibitory concentration (MIC) values of the bacteria were determined using the microdilution method. A time-dependent increase was observed in the number of bacteria that developed resistance and increased MIC values. Consistent with the ciprofloxacin resistance observed after exposure, an increase in norA, norB/C, gyrA, gyrB, parC, and parE gene expression was observed. In addition to aluminum chlorohydrate exposure, oxacillin resistance was observed in all test bacteria in the group only subcultured in the medium, suggesting that phenotypic resistance cannot be correlated with chemical exposure in light of these data. The increase in mecA gene expression in selected test bacteria that acquired resistance to oxacillin after exposure compared with control groups suggests that the observed resistance may have been related to aluminum chlorohydrate exposure. To our knowledge, this is the first time in the literature that the effects of aluminum chlorohydrate as an antiperspirant on the development of antibiotic resistance in Staphylococcus epidermidis have been reported.
Effects of Different Progesterone Doses on the Concentrations of Proinflammatory and Anti-inflammatory Cytokines in Pregnant Women With Threatened Abortion
(Springernature, 2021) Kirici, Pinar; Tanriverdi, Elif Seren
Background and objective This study aimed to investigate how different doses of progesterone influence the concentrations of interleukin-6 (IL-6) and tumor necrotizing factor-alpha (TNF-alpha), which are proinflammatory cytokines, as well as that of IL-10, which is an anti-inflammatory cytokine, in pregnant women with threatened abortion. Materials and methods This is a prospective, single-center, randomized controlled trial conducted with 221 patients with a threatened abortion diagnosis. Group 1 consisted of IL-6, IL-10, and TNF-alpha values in pre-treatment blood samples from 221 patients diagnosed with imminent abortion. Group 2 included 81 patients who received natural oral 100 mg micronized progesterone MP twice a day for two weeks. Group 3 included 83 patients who were administered oral 200 mg of natural micronized progesterone MP twice a day for two weeks. Group 4 included 57 patients who received oral 200 mg of natural micronized progesterone MP twice a day for two weeks, and one depot progesterone was added to the treatment by administering it at a dosage of 500 mg/day intramuscularly. Results IL-6 values between groups were lower in group 4 compared to group 3 (p=0.007). When IL-10 values were compared between the groups, the IL-10 ratio was highest in group 4 and lowest in group 2 (p<0.001, p=0.003, p<0.001). When the TNF-alpha values between the groups were compared, the value in group 4 was decreased compared to groups 1 and 2 (p=0.031, p<0.001). In the logistic regression analysis, the IL-6 value above 12.01 increased the abortion imminens rate 1.01 times, and a TNF-alpha value above 11.04 increased the abortion imminens rate 1.21 times. Conclusion Progesterone used to treat imminent abortion reduces the levels of proinflammatory cytokines, such as IL-6 and TNF-alpha, while increasing those of anti-inflammatory cytokine IL-10 in proportion to the dose administered. Progesterone can prevent imminent abortion by generating an anti-inflammatory environment.

Yazar "Tanriverdi, Elif Seren" seçeneğine göre listele

Sayfa Başına Sonuç

Sıralama seçenekleri