Yazar "Tarhan, Abbas" seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • Küçük Resim Yok
    Öğe
    Chemical composition, and antioxidant, antimicrobial, and cytotoxicity properties of Silene latifolia
    (Springer International Publishing, 2026) Gökkaya, İçim; Hepokur, Ceylan; Aykaç, Okan; Ulama, Sena Nur; Bal, Halil; Cakir, Oğuz; Tarhan, Abbas
    Silene species have various pharmacological properties, including antimicrobial, antioxidant, anti-inflammatory, antitumoral, adaptogenic, immunomodulatory, hepatoprotective, and cytotoxic activities. This study assessed the antibacterial, antioxidant, and cytotoxic activities and the chemical profile of crude methanol extract and sub-extracts prepared from the aerial parts of Silene latifolia for the first time. After a 24-h incubation, the methanol extract exhibited the highest activity against MCF-7 cancer cell line (IC50: 19.25 ± 0.05 µg/mL), whereas the water sub-extract showed the greatest activity against MDA-MB-231 cancer cell line (IC50: 15.20 ± 0.47 µg/mL). After a 48-h incubation, the extracts having the strongest cytotoxic effects against MCF-7 and MDA-MB-231 cells were the water sub-extract (IC50: 14.52 ± 0.86 µg/mL) and the methanol crude extract (IC50: 12.08 ± 0.78 µg/mL), respectively. The methanol extract exhibited the most potent radical-scavenging activity, enhancing total antioxidant status and reducing total oxidant status. The extracts had no antibacterial or antifungal activity against the tested microorganisms. GC–MS analysis revealed that oleic acid, elaidic acid, palmitic acid, and their derivatives were abundant in sub-extracts. The LC–MS/MS analysis demonstrated that the water sub-extract included fumaric acid, quinic acid, aconitic acid, acacetin, and luteolin. This study highlighted that S. latifolia exhibited high antioxidant activity, and that it had a potent cytotoxic effect against breast cancer cells with a high selectivity index. © The Author(s), under exclusive license to Società Botanica Italiana onlus 2026.
  • Küçük Resim Yok
    Öğe
    Investigating the Cytotoxic Potential of Omphalodes cappadocica (Willd.) DC. on Breast Cancer Cells by Phytochemical-Based Molecular Docking Studies: A Comparative Study of Aerial Parts and Roots
    (Wiley-V C H Verlag Gmbh, 2026) Gokkaya, Icim; Hepokur, Ceylan; Aykac, Okan; Karaman, Elif; Cakir, Oguz; Tarhan, Abbas; Yilmaz, Mustafa Abdullah
    This study aims to evaluate the potential effects of Omphalodes cappadocica on a breast cancer cell line for the first time. The methanol extract of the aerial parts (Ae-M) exhibited the strongest cytotoxicity against MCF-7 and MDA-MB-231 cells after 48 h of incubation, with IC50 values of 14.08 +/- 0.05 and 10.63 +/- 0.01 mu g/mL, respectively. The root methanol extract (Ro-M) exhibited significant activity in the MCF-7 and MDA-MB-231 cell lines during 48 h of incubation, with IC50 values of 19.25 +/- 0.66 and 16.38 +/- 0.02 mu g/mL, respectively. Quantitative analysis by LC-MS/MS revealed that the main constituents of Ae-M were rosmarinic, quinic, and vanillic acids. Quinic and fumaric acids were identified in Ro-M. Quinic acid had a strong affinity for the target proteins PDB 3ERT, PDB 3HNG, and PDB 3KMU, with docking scores of -9.2, -7.8, and -6.6 kcal/mol, respectively. The docking score for rosmarinic acid for the target protein PDB 3HNG was determined to be -9.7 kcal/mol. The findings suggest that O. cappadocica may serve as a source for the development of anticancer agents.