Yazar "Tasdemir, Seda" seçeneğine göre listele

Listeleniyor 1 - 8 / 8
  • Küçük Resim Yok
    Öğe
    Beneficial effects of apricot-feeding on myocardial ischemia-reperfusion injury in rats
    (Pergamon-Elsevier Science Ltd, 2009) Parlakpinar, Hakan; Olmez, Ercument; Acet, Ahmet; Ozturk, Feral; Tasdemir, Seda; Ates, Burhan; Gul, Mehmet
    The present study was undertaken to evaluate the cardio-protective potential of apricot-feeding in the ischemia-reperfusion (I/R) model of rats in vivo. Rats were divided into three groups of 12 rats each. Group 1 was fed with a standard rat chow, groups 2 and 3 were fed with a standard rat chow supplemented with 10% or 20% dried apricot during 3 months before the beginning of I/R studies. To produce I/R, the left main coronary artery was occluded for 30 min, followed by 120 min reperfusion, in anesthetized rats. Infarct sizes were found significantly decreased in 10% (55.0 +/- 4.3%) and 20% (57.0 +/- 2.9%) apricot-fed groups compared to control group (68.7 +/- 2.0%). Light and electron microscopic evaluations of hearts also demonstrated similar beneficial effects on I/R injury in apricot-fed both groups. Total phenolic contents, DPPH radical scavenging and ferric-reducing power as in vitro antioxidant capacities of rat chows were significantly increased after supplementation with apricot for each ratio. Cu, Zn Superoxide dismutase (Cu, Zn SOD) and catalase (CAT) activities were increased, and lipid peroxidation was decreased significantly in the hearts of 20% apricot-fed group after I/R. In conclusion, we clearly demonstrated in vivo cardio-protective activity of apricot-feeding related to its antioxidant phenolic contents in rats subjected to myocardial I/R. (C) 2009 Elsevier Ltd. All rights reserved.
  • Küçük Resim Yok
    Öğe
    DOSE DEPENDENT EFFECTS OF CAFFEIC ACID PHENETHYL ESTER ON HEART RATE AND BLOOD PRESSURE IN RATS
    (Modestum Ltd, 2005) Iraz, Mustafa; Fadillioglu, Ersin; Tasdemir, Seda; Ates, Burhan; Erdogan, Selim
    Aim: Caffeic acid phenethyl ester (CAPE) is one of the major components of honeybee propolis and its structure is similar to flavonoids. The molecular mechanisms of the effects of CAPE on various systems including cardiovascular system have not been known well. The aim of the present study was to investigate the short term dose dependent in vivo cardiovascular effects including heart rate and blood pressure changes induced by CAPE in Sprague Dawley rats. Methods: The rats were anaesthetized and randomly divided into six groups (n: 6 rats) as follows: the first two groups of rats were injected 0.9% NaCl or 10% alcohol; the other groups were injected 1 mg kg(-1), 5 mg kg(-1) 10 mg kg(-1) or 20 mg kg(-1) CAPE i.v. Results: CAPE injection caused decrease in mean blood pressure (MBP) up to 20 sec. for 1 mg CAPE group and up to 2 min for 5 and 10 mg CAPE groups. On the other hand, heart rate (HR) was found to be decreased up to 10 min. for 10 mg CAPE group Conclusion: CAPE causes decrease in both HR and MBP and may affect conduction velocity and contractility in heart due to possible effects on neuronal transmission.
  • Küçük Resim Yok
    Öğe
    Effect of endogen-exogenous melatonin and erythropoietin on dinitrobenzene sulfonic acidinduced colitis
    (Wiley, 2013) Tasdemir, Seda; Parlakpinar, Hakan; Vardi, Nigar; Kaya, Emin; Acet, Ahmet
    Inflammatory bowel disease has been linked to elevated T cells. Excessive production of reactive oxygen species and apoptosis are known to be accompanied by intestinal inflammation. This study was designed to investigate the effects of melatonin (MEL) and erythropoietin (EPO), which is a known anti-inflammatory and antiapoptotic agent, in dinitrobenzene sulfonic acid (DNBS)induced colitis in pinealectomized (Px) rats. In microscopically results, epithelial and goblet cell loss, absence of crypts, and increased colonic caspase-3 activity were observed in the DNBS group. Also, in flow cytometric analysis, the percentage of CD4+ T cells was highest in the DNBS group. Treatment with MEL or EPO had a curative effect on DNBS-induced colitis. The MEL+EPO groups showed significantly greater improvement when compared with the other treatment groups. Our results indicate that the combination of EPO and MEL may exert more beneficial effects than either agent used alone.
  • Küçük Resim Yok
    Öğe
    Effects of Ozone Therapy on Cyclophosphamide-induced Urinary Bladder Toxicity in Rats
    (Canadian Soc Clinical Investigation, 2013) Tasdemir, Seda; Tasdemir, Cemal; Vardi, Nigar; Ates, Burhan; Taslidere, Elif; Karaaslan, Merve G.; Sapmaz, Hilal I.
    Purpose: This study investigated the efficacy of ozone therapy (OT) in a rat model of cyclophosphamide-induced hemorrhagic cystitis (HC). Methods: Forty Wistar Albino male rats were divided into five groups: sham, OT, cyclophosphamide (CP), OT+CP and CP+OT. Hemorrhagic cystitis (HC) was induced by intraperitoneal (i.p) administration a single dose of 100 mg/kg CP. OT was performed once daily for three days. the CP+OT group received OT (0.2 mg/kg) i.p 24 h after CP administration. CP was injected to the OT+CP group the day after the third course of OT. All animals were killed four days after CP administration. Bladder injury and oxidative stress parameters were determined from tissue samples. Results: We found small, but non-statistically significant biochemical and histological changes in the animals treated with OT alone. CP administration induced cystitis, as manifested by a marked loss of urothelial cells, as well as hemorrhaging and edema in the bladder as determined by histopathological examination. It also caused a significant decrease in the endogenous antioxidant compound glutathione (GSH) and elevation of lipid peroxidation, and nitric oxide (NO) and myeloperoxidase (MPO) levels in the rats' urinary bladder tissue. OT was able to ameliorate these changes; however these effects were prominent in the CP+OT group when compared with the OT+CP group.: For example, the NO level in the CP+OT group was 68% of the OT+CP group (p < 0.05). Conclusion: OT prevented CP-induced urothelial damage by diminishing bladder oxidative stress, inflammation and NO levels. OT may help to ameliorate bladder damage induced by CP in the clinical setting.
  • Küçük Resim Yok
    Öğe
    Intravesical hyaluronic acid and chondroitin sulfate alone and in combination for urinary tract infection: Assessment of protective effects in a rat model
    (Wiley, 2012) Tasdemir, Seda; Tasdemir, Cemal; Vardi, Nigar; Yakupogullari, Yusuf; Duman, Yucel; Parlakpinar, Hakan; Sagir, Mustafa
    Objective: To determine the protective effects of hyaluronic acid and chondroitin sulfate in treating urinary tract infections in a rat model. Methods: A total of 28 rats, which were induced with urinary tract infections through intravesical administration of Escherichia coli, were included in the study. By random selection, they were equally divided into four groups as control (no treatment), hyaluronic acid, chondroitin sulfate and hyaluronic acid + chondroitin sulfate. Bacteriological cultures of the urine and bladder tissue samples were carried out, and the data for each group were statistically compared. Results: In the urine cultures, there were significant differences in median bacterial growth rates in hyaluronic acid (5 x 103 cfu/mL) and chondroitin sulfate (1 x 104 cfu/mL) groups relative to the control group (5 x 104 cfu/mL). However, a significantly lower rate of bacterial colony growth was observed in the hyaluronic acid + chondroitin sulfate group (8 x 102 cfu/mL; P < 0.05). In the bladder tissues, statistically significant decreases in median bacterial growth rates were detected in the hyaluronic acid and hyaluronic acid + chondroitin sulfate groups (both 0 cfu/mg tissue; P < 0.05). Also, transitional epithelium damage decreased in the treatment groups. However, this effect was prominent in hyaluronic acid + chondroitin sulfate group. Conclusion: Our experimental findings show that the hyaluronic acid + chondroitin sulfate combination has a potential benefit in reducing the bacterial load in urine and the thickness of the transitional epithelium.
  • Küçük Resim Yok
    Öğe
    Penile alterations at early stage of type 1 diabetes in rats
    (Brazilian Soc Urol, 2017) Tao, Mingfang; Tasdemir, Cemal; Tasdemir, Seda; Shahabi, Ali; Liu, Guiming
    Objective: Diabetes affects the erectile function significantly. However, the penile alterations in the early stage of diabetes in experimental animal models have not been well studied. We examined the changes of the penis and its main erectile components in diabetic rats. Materials and methods: Male Sprague-Dawley rats were divided into 2 groups: streptozotocin (STZ)-induced diabetics and age-matched controls. Three or nine weeks after diabetes induction, the penis was removed for immunohistochemical staining of smooth muscle and neuronal nitric oxide synthase (nNOS) in midshaft penile tissues. The cross-sectional areas of the whole midshaft penis and the corpora cavernosa were quantified. The smooth muscle in the corpora cavernosa and nNOS in the dorsal nerves were quantified. Results: The weight, but not the length, of the penis was lower in diabetics. The cross-sectional areas of the total midshaft penis and the corpora cavernosa were lower in diabetic rats compared with controls 9 weeks, but not 3 weeks after diabetes induction. The cross-sectional area of smooth muscle in the corpora cavernosa as percentage of the overall area of the corpora cavernosa was lower in diabetic rats than in controls 9 weeks, but not 3 weeks after diabetes induction. Percentage change of nNOS in dorsal nerves was similar at 3 weeks, and has a decreased trend at 9 weeks in diabetic rats compared with controls. Conclusions: Diabetes causes temporal alterations in the penis, and the significant changes in STZ rat model begin 3-9 weeks after induction. Further studies on the reversibility of the observed changes are warranted.
  • Küçük Resim Yok
    Öğe
    Protective Effect of Infliximab on Ischemia/Reperfusion-Induced Damage in Rat Kidney
    (Taylor & Francis Ltd, 2012) Tasdemir, Cemal; Tasdemir, Seda; Vardi, Nigar; Ates, Burhan; Parlakpinar, Hakan; Kati, Bulent; Karaaslan, Merve Goksin
    Objective: To investigate the protective effect of infliximab on ischemia-reperfusion (I/R) injury of the rat kidney. Methods: Twenty-eight male Wistar albino rats were divided into four groups: sham-operated, I/R, I/R with infliximab administered before ischemia [I/R + infliximab (bi)], and I/R with infliximab administered before reperfusion [I/R + infliximab (br)]. After a right nephrectomy to produce damage, the left renal vessels were occluded for 60 min, followed by 24-h reperfusion in rats. Changes in the rat kidney were observed by measuring the tissue levels of malondialdehyde (MDA), myeloperoxidase (MPO), glutathione (GSH), and superoxide dismutase (SOD) and by evaluating hematoxylin-eosin (H&E)-stained and periodic acid-Schiff (PAS) sections. Results: The MDA and MPO levels in the I/R group were significantly higher than in the other groups (p < 0.05), and the SOD and GSH levels in the I/R + infliximab (bi) and I/R + infliximab (br) groups were significantly higher than in the I/R group (p < 0.05). However, histological examination revealed that the I/R + infliximab (bi) group and the I/R + infliximab (br) group had significantly fewer tubular changes and interstitial inflammatory cell infiltration than the I/R group. Conclusion: These results show that infliximab may protect against I/R injury in the rat I/R model.
  • Küçük Resim Yok
    Öğe
    Protective effects of ghrelin on kidney tissue in rats with partial ureteral obstruction
    (Tubitak Scientific & Technological Research Council Turkey, 2019) Cimen, Serhan; Tasdemir, Cemal; Vardi, Nigar; Ates, Burhan; Tasdemir, Seda; Ozaydogdu Cimen, Ayla
    Background/aim: The aim was to investigate the protective and therapeutic effects of ghrelin, which has antioxidant and antiinflammatory activity, on preventing kidney damage that occurs by induced partial ureteral obstruction in rats. Materials and methods: Twenty-eight adult male rats were included in the study, and the rats were divided into 4 groups. After the laparotomy operation on the sham group, the ureter was identified in the retroperitoneal area and was duly sutured (n = 7). Ghrelin was administered for seven days intraperitoneally, and after the nephrectomy performed on the 15th day, the rats were sacrificed (n = 7). A partial ureteral obstruction was performed after the laparotomy on the PUO group. The rats were sacrificed after the nephrectomy operation performed on the 15th day (n = 7). A partial ureteral obstruction was formed after the laparotomy followed by seven days of waiting in the PUO i ghrelin group. Ghrelin was given in the dose of 10 ng/kg per day intraperitoneally for the next 7 days, and the rats were sacrificed after the nephrectomy operation performed on the 15th day (n = 7). All groups were evaluated for histological damage and catalase, superoxide dismutase, total glutathione, malondialdehyde, and myeloperoxidase levels were measured in the same tissues. Results: When the 2nd group and the sham group were compared histologically, it was observed that the damage had increased by a statistically significant level in the partial ureteral obstruction group (P 0.001). When the group which was ghrelin-treated after the partial ureteral obstruction was compared to the group with just partial ureteral obstruction, the histopathological changes were found to decrease significantly in that group (P = 0.001). While the statistical significance of the levels of CAT, GSH, and MP() enzymes was detected among biochemical changes in the 2nd group when compared to the sham group (P < 0.01), the 3rd group showed a statistically significant difference in the levels of SOD and GSH enzymes compared to the 4th group (P < 0.05). Conclusion: Ghrelin administration to rats after the formation of an experimental partial unilateral ureteral obstruction reduces tissue damage due to ghrelin's antiinflammatory and antioxidant effects. Ghrelin administration may prevent tissue damage biochemically and histopathologically in obstructive uropathy cases.