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Öğe 4-Methylsulfonylbenzyl Substituted N-Heterocyclic Carbene-Ruthenium (II) Complexes: Design, Synthesis, Characterization, and Anticancer Activities(Wiley-V C H Verlag Gmbh, 2026) Pasahan, Aziz; Taskin, Irmak Icen; Gurses, Canbolat; Sever, Meryem Ruveyda; Karabiyik, Hande; Karabiyik, Hasan; Gok, YetkinIn this study, we report the synthesis and anticancer activities of new ruthenium complexes with N-heterocyclic carbene (NHC) ligands, which are of great significance for drug delivery research. For this reason, 4-methylsulfonylbenzyl-substituted benzimidazole-functionalized Ru(II)NHC complexes were synthesized in our research. The characterization of these new complexes were performed using appropriate spectroscopic methods (1H NMR, 13C NMR, and FT-IR) and elemental analysis techniques. The crystal structure of complex 1c was obtained using single crystal X-ray diffraction. MTT method was used to understand in vitro anticancer activities of the complexes against MCF-7 (breast cancer), HCT-116 (colon cancer), SH-SY5Y (brain cancer), and HeLa (cervical cancer) cell lines. Based on the IC50 values determined by MTT assay, the most effective complex was identified as 1 h. DNA binding analyses were carried out using agarose gel electrophoresis, revealing that 1 h weakly interacted with DNA. Additionally, the effects of 1 h on cell cycle progression and apoptosis in the SH-SY5Y cell line were examined using flow cytometry. The results indicated that 1 h induced G0/G1 phase accumulation and increased apoptotic cell death. These findings suggest that the synthesized complex 1 h has a significant anticancer potential.Öğe Bazı Fumaria L. (Papaveraceae) Türlerinin Metanolik Ekstraktlarının Kanser Hücre Hatları Üzerindeki İn Vitro Sitotoksik Etkileri(2024) Sancar, Pelin Yılmaz; Taskin, Irmak IcenFumaria cinsi (Papaveraceae) halk arasında “Şahtere” olarak bilinir. Son derece zengin bileşiklere sahip olduğu kanıtlanmış olan cins, halk arasında birçok rahatsızlığın tedavisinde kullanılmaktadır. Bu çalışmada, Fumaria asepala Boiss. ve Fumaria schleicheri Soy.-Will. subsp. microcarpa Boiss. ex Hausskn. taksonlarının toprak üstü kısımlarının metanolik ekstraktının Beas-2B, sh-sy5, HCT116 ve A549 hücre hatlarına karşı sitotoksik özellikleri araştırıldı. Kanser hücrelerinin canlılığındaki değişiklikler 3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolyum bromür (MTT) yöntemi kullanılarak tespit edildi. Sonuçlar, F. asepala ve F. schichleiri subsp. microcarpa’nın metanolik ekstraktının özellikle kolon (HCT-116) kanseri hücrelerinin hücre canlılığında istatistiksel olarak anlamlı bir azalma olduğunu gösterirken, akciğer (A549) kanseri ve beyin (sh-sy5) kanseri hücreleri üzerinde sitotoksik etkinlik sağlamamıştır. Ayrıca bitkisel ekstraktlar, sağlıklı akciğer hücreleri (Beas-2B) üzerinde ise herhangi bir toksik etki yaratmamıştır.Öğe Chenopodium foliosum Metanol Ekstraktının Biyolojik Aktivitelerinin Araştırılması(2024) Yılmaz Sancar, Pelin; Taskin, Irmak Icen; Vazgeçen, Özge; Sever, Meryem RüveydaChenopodium cinsi Türkiye dahil hemen hemen dünyanın her yerinde yayılış göstermektedir. Bu cinse ait türler farklı ülkelerin geleneksel tıbbında çeşitli hastalıkların tedavisinde kullanılmaktadır. Chenopodium cinsinin farklı türleri anti-kanser, anti-mantar ve anti-bakteriyel etkiler göstermektedir. Chenopo-dium foliosum (C. folisoum) ile ilgili literatürde büyük bir boşluk bulunmak-tadır. Bu çalışmada C. folisoum bitkisinin metanol ekstraktının biyolojik ak-tiviteleri araştırıldı. Anti-kanser aktivitesi, HCT-116 ve A549 hücre hatlarında 3-(4,5-Dimetiltiazol-2-il)-2,5-difeniltetrazolyum bromür (MTT) analizi kullanılarak değerlendirildi. Anti-bakteriyel ve ati-fungal aktivitesi minimum inhibisyon konsantrasyonu (MIC) tahlili ile incelenmiştir. C. folisoum'un metanol ekstraktı, HCT116 ve A549 hücre hatlarının hücre canlılığını sırasıyla %73,4 ± %15,3 ve %78,7 ± %2,3'e düşürdüğü saptanmıştır. C. folisoum ayrıca candida albicans'ın (C. albicans) hücre canlılığını %91,2'ye düşürdüğü saptanmıştır. Elde edilen bulgular C. folisoum metanol ekstraktının HCT116, A549 ve C. albicans'a karşı hem antikanser hem de antifungal etkiye sahip olduğunu göstermektedir.Öğe Design, Synthesis, Characterization, and Antitumor Activities of Benzimidazole-functionalized Organoruthenium Complexes Bearing Fluorine Group(Bentham Science Publ Ltd, 2025) Pasahan, Ramazan; Demirci, Ozlem; Taskin, Irmak Icen; Pasahan, Aziz; Sever, Meryem Ruveyda; Gok, Yetkin; Aktas, AydinBackground This work presents the synthesis of Ru(II)NHC complexes bearing a series of 4-fluorobenzyl group. These complexes have been characterized by a variety of spectroscopic methods (1H NMR, 13C NMR, and FTIR) and by elemental analysis techniques.Methods These complexes' antitumor activities against SH-SY5Y (human neuroblastoma) and HCT116 (human colon cancer) were investigated by 3-(4,5-dimethylthiazole-2-yl)-2,5-biphenyl tetrazolium bromide (MTT) assay.Results The results showed that all the synthesized complexes exhibited significant cytotoxic effect with low IC50 values 15 +/- 0.57, 15.26 +/- 0.71, 7.64 +/- 0.30, 27.66 +/- 0.36 and 14.45 +/- 0.84 (mu g/mL) respectively.Conclusion Furthermore, apoptosis assessed by double labeling with Annexin V-FITC/PI indicated that complexes 1b and 1d can effectively induce apoptosis and inhibit cell proliferation at the S phase in SH-SY5Y cells. Taken together, Ru(II)NHC complexes containing the 4-fluorobenzyl group have significant potential for the development of novel, highly effective anticancer agents.Öğe Designing of rapid assay for the detection of RdRp/Orf1ab specific to SARS-CoV-2(Elsevier, 2023) Derin, Dilek Cam; Gultekin, Enes; Taskin, Irmak Icen; Otlu, Baris; Oktem, Huseyin AvniSARS-CoV-2 is still threat and mostly used detection method is real time reverse transcriptase polymerase chain reaction (rRT-PCR) for the open reading frame (Orf1ab), RNA-dependent RNA polymerase (RdRp), nucleocapsid (N) and envelope (E) genes of virus. However, rRT-PCR may have false negative rate for the nucleic acid detection. Since the RdRp/Orf1ab has high sensitivity for the molecular detection, two sandwich models, Model 1A-Model 1B, based on hybridization on lateral flow assay (LFA) were designed here and applied with the synthetic and clinical samples of RdRp/Orf1ab. In this purpose colloidal gold nanoparticles (AuNPs) were used as label. Membranes having different flow rate, three oligonucleotide probe concentrations and running buffers were used. Although synthetic target sequence was recognized by all the LFAs, PCR products obtained from either the synthetic plasmid DNA or oro/nasopharyngeal swabs were detected by Model 1 A using W12 mem-brane. Designed strip assays detected the RdRp/Orf1ab of the clinical samples as 100% sensitivity and specifity. It means that they might be used for the detection of virus and can be modified for the recognition of mutant genes of virus. These findings also demonstrated the importance of membranes, sandwich models, probe con-centrations and sample contents for developing LFAs for viral detection.Öğe Development of nucleic acid based lateral flow assays for SARS-CoV-2 detection(Soc Bioscience Bioengineering Japan, 2023) Derin, Dilek Cam; Gultekin, Enes; Taskin, Irmak Icen; Yakupogullari, YusufSARS-CoV-2 is still threat for humanity and its detection is crucial. Although real time reverse transcriptase poly-merase chain reaction is the most reliable method for detection of N protein genes, alternative methods for molecular detection are still needed. Thus, lateral flow assay models for 2019-nCoV_ N3 were developed for molecular detection. Briefly, gold nanoparticles were used as label and three sandwich models (1A, 1B, and 1.2) were designed. Prob con-centrations on gold nanoparticles, types of sandwich model and membrane, limit of detection of target gene and buffer efficiency were studied. Model 1B has shown the best results with M170 membrane. Lower limit of detection was achieved by model 1.2 as 5 pM. All parameters have significant role for molecular detection of SARS-CoV-2 by lateral flow assays, and these results will be useful for nucleic acid based lateral flow assays for viral detection or multiple detection of mutated forms in various detection systems.(c) 2022, The Society for Biotechnology, Japan. All rights reserved.Öğe Elevated Expression of ADAMTS-12 in Maternal and Umbilical Cord Serum of Placenta Accreta Spectrum(Taylor & Francis Inc, 2023) Taskin, Irmak IcenObjective: We investigated a disintegrin and metalloproteinase with thrombospondin type 1 motif-12 (ADAMTS-12) levels in both fetal umbilical cord and maternal blood of pairs diagnosed with placenta accreta spectrum (PAS).Method: We analyzed ADAMTS-12 level by ELISA in 160 samples that included 30 control pairs (30 maternal and 30 umbilical cord serums), 20 pairs in the placenta previa (PP), and 30 pairs in the PAS group (30 maternal and 30 umbilical cord serums).Results: There were increased serum levels of ADAMTS-12 in maternal serum (p = 0.037) and umbilical cord serum level (p = 0.004) of PAS group compared with the PP and healthy control groups. There was a positive correlation between maternal ADAMTS-12 and fetal ADAMTS-12 serum levels (r = 0.53, p = 0.002).Conclusion: Our findings indicate that ADAMTS-12 could be a -diagnostic biomarker for PAS.Öğe Expression of sirtuin 2 and 7 in placenta accreta spectrum(Assoc Medica Brasileira, 2023) Taskin, Irmak Icen; Gurbuz, Sevim; Icen, Mehmet Sait; Derin, Dilek Cam; Findik, Fatih MehmetOBJECTIVE: This study aimed to investigate the expression levels of sirtuin 2 and sirtuin 7 in the placenta accreta spectrum to reveal their role in its pathogenesis.METHODS: A total of 30 placenta accreta spectrum, 20 placenta previa, and 30 controls were experienced. The sirtuin 2 and sirtuin 7 expression levels in the placentas of these groups were determined by Western blot. sirtuin 2 and sirtuin 7 serum levels in the maternal and fetal cord blood were examined by enzyme-linked immunosorbent assay.RESULTS: It was found that sirtuin 7 in placenta accreta spectrum was significantly lower in the placenta compared to the control and placenta previa groups (p<0.05). However, a significant difference was not observed between the sirtuin 2 and sirtuin 7 levels in the maternal and fetal cord serum samples of those three groups (p>0.05).CONCLUSION: Sirtuin 7 may play an important role in the formation of placenta accreta spectrum. The effect of decreased expression of sirtuin 7 might be tissue-dependent in the placenta accreta spectrum and needs to be investigated further.Öğe Expression of sirtuins 1 in placenta, umbilical cord, and maternal serum of patients diagnosed with placenta accreta spectrum(Assoc Medica Brasileira, 2024) Taskin, Irmak Icen; Gurbuz, Sevim; Icen, Mehmet Sait; Derin, Dilek Cam; Findik, Fatih Mehmet; Deveci, EnginOBJECTIVE: Placenta accreta spectrum (PAS) is defined as the attachment of the placenta to the uterine wall in varying degrees. However, the studies have explored that the underlying molecular mechanisms of the PAS are very limited. Sirtuins 1 (SIRT1) is associated with placental development by controlling trophoblast cell invasion and remodeling of spiral arteries. We aimed to determine the expression level of SIRT1 in placentas, and maternal and umbilical cord serum of patients with PAS. METHODS: In total, 30 individuals in control, 20 patients in the placenta previa group, and 30 patients in the PAS group were included in this study. The expression levels of SIRT1 in the placentas were determined by Western blot and immunohistochemistry. Serum levels of SIRT1 in maternal and umbilical cord blood were determined by ELISA. RESULTS: SIRT1 was significantly lower in placentas of the PAS. However, maternal and umbilical cord serum samples were not significantly different between groups. CONCLUSION: SIRT1 may play an important role in the pathogenesis of the PAS.Öğe Investigation of probiotic properties of Lactiplantibacillus plantarum isolated from hawthorn vinegar(Taylor & Francis Ltd, 2024) Taskin, Irmak IcenProbiotic microorganisms are gaining attention because of their beneficial effects on human health. Vinegar is produced by fermenting carbohydrate-rich foods with yeasts and acetic acid bacteria. Hawthorn vinegar is notable for its high concentration of aromatic compounds, amino acids, vitamins, minerals, and organic acids. In this study, probiotic bacteria was isolated from traditionally fermented hawthorn vinegar. Following genotypic characterization, the isolated bacteria was evaluated for its capacity to grow and survive in a low pH environment, antibiotic susceptibility, surface adhesion qualities, cholesterol precursor degradation, and adherence to HCT116 cells. The data indicate that the isolated strain is Lactiplantibacillus plantarum (L. plantarum IIT), and it exhibits probiotic potential. The strain can break down four distinct cholesterol precursors and was resistant to N30, K30, VA30 and S10 antibiotics. Additionally, it demonstrates a high adhesion capacity to HCT116 cells (96.63%) and exhibits cytotoxic effects on these cells.Öğe Novel ruthenium(II)oxothiazolidine complexes: Design, synthesis, characterization, DNA binding and anticancer activity(Elsevier, 2026) Aktas, Aydin; Taskin, Irmak Icen; Sevincek, Resul; Haroon, Muhammad; Derin, Dilek Cam; Taskin-Tok, Tugba; Sever, Meryem RuveydaCancer remains one of the leading causes of death worldwide, making the search for effective anticancer agents a critical area of research. In recent years, ruthenium-based compounds have gained significant attention due to their potential as novel candidates for cancer treatment. This report aims to explore the synthesis and anticancer properties of the Ru(II)oxothiazolidine complexes. All complexes have been prepared from ligands containing hydrazinyl-oxothiazolidine moiety and [RuCl2(p-cymene)]2 substrate. The basic skeleton of the complexes is justified with 1H-, 13C-NMR, and FTIR spectroscopic methods. The proposed structures of the complexes were further confirmed with elemental analysis. The crystal structure of the complex 2a has been determined by using single-crystal X-ray diffraction. Asymmetric unit of structure contains two crystallographically independent molecules, dichloromethane and two chloride anions. All complexes exhibited strong activity against MCF-7 (breast cancer) and HCT-116 (colon cancer) cancer cell lines better than standard anticancer drug cisplatin. The complex 2a showed the highest anticancer efficacy against MCF-7 (IC50: 13.89 mu M) and HCT-116 (IC50: 14.02 mu M). DNA binding study also demonstrates that all complexes have an interaction ability to DNA. Ethidium bromide fluorescence quenching assay revealed moderate DNA binding for complex 2a suggesting partial intercalation or groove binding with ct-DNA. Meanwhile, molecular docking simulations of potent rutheniumbased oxothiazolidine complexes (1a, 1c, and 2a) against breast (MCF-7) and (1a, 1c, and 2a) colon (HCT116) cancer cell models were carried out. The findings suggest that complex 2a is the best candidate complex for both cancers. Furthermore, complexes 1a and 1c demonstrated potent cytotoxic activity against MCF-7 breast cancer cells, whereas complexes 1b and 2d exhibited significant cytotoxic effects against HCT-116 colon cancer cells.Öğe Phytogenic Silver and Copper Nanoparticles from Morus nigra L. Leaf as Multifunctional Agents: Antioxidant, Antidiabetic, Antimicrobial, and Anticancer Potency(2025) Derin, Dilek Çam; Baltaci, Cemalettin; Akmeşe, Osman; Taskin, Irmak Icen; Karpuz, Omer; Gültekin, Enes; Sever, Meryem RüveydaThe growing need for eco-friendly nanomaterials has directed attention toward plant-based green synthesis as a sustainable approach for biomedical applications. This study reports the biosynthesis of silver (YAgNPs) and copper nanoparticles (YCuNPs) using Morus nigra L. leaf extract and evaluates their multifunctional properties. The nanoparticles were produced under alkaline aqueous conditions, and their crystalline nature and nanoscale morphology were confirmed by standard structural and elemental analyses. Both YAgNPs and YCuNPs exhibited enhanced antioxidant capacities compared to the crude extract. YAgNPs showed the highest ferric reducing antioxidant power (250.33 mg FeSO₄/g), while YCuNPs demonstrated superior DPPH radical scavenging activity (114.12 mg AAE/g). In antidiabetic assays, YAgNPs strongly inhibited α-amylase (IC₅₀ = 112.02 μg/mL) and α-glucosidase (IC₅₀ = 38.27 μg/mL), approaching the potency of acarbose. Antimicrobial analysis revealed distinct activity profiles: YAgNPs were highly effective in broth cultures with low MIC and MBC values (0.09–0.18 mg/mL against B. subtilis and E. coli), whereas YCuNPs achieved larger inhibition zones in diffusion assays. Checkerboard testing confirmed synergistic interactions between nanoparticles and antibiotics, with FICI values ≤0.5 against E. faecium, E. coli, and P. aeruginosa, highlighting their ability to enhance antibiotic efficacy. YAgNPs exhibited notable cytotoxic effects against HeLa and HCT116 cancer cell lines, with IC₅₀ values of 9.84 and 6.83 μg/mL, respectively. In contrast, YCuNPs displayed weaker anticancer activity. Overall, these findings demonstrate that M. nigra derived nanoparticles combine antioxidant, antidiabetic, antimicrobial, and anticancer activities with synergistic antibiotic-enhancing effects, underscoring their potential as sustainable, multifunctional therapeutic agents.Öğe Prediction of newly synthesized heparin mimic's effects as heparanase inhibitor in cancer treatments via variational quantum neural networks(Elsevier Sci Ltd, 2025) Kocabay, Samet; Acar, Erdi; Memis, Samet; Taskin, Irmak Icen; Sever, Meryem Ruveyda; Sener, RamazanCancer remains a leading global cause of death, primarily driven by the uncontrolled proliferation of abnormal cells. Malignant tumors, such as carcinomas, originate from unchecked epithelial cell growth and produce growth factors like FGF and VEGF, which promote angiogenesis and tumor progression through heparanasemediated degradation of heparan-sulfate proteoglycans. Chitosan and its derivatives have shown promise in inhibiting tumor growth and metastasis. This study aims to investigate newly synthesized sulfated chitosan oligomers as heparin mimics to inhibit heparanase, evaluating their cytotoxic effects on SH-SY5Y, HCT116, A549, and MDA-MB-231 cancer cell lines. Moreover, it seeks to leverage a variational quantum neural network (VQNN) to predict and validate cytotoxicity outcomes, integrating quantum computing methods into evaluating novel anticancer compounds. The VQNN algorithm was applied to analyze the anticancer effects of sulfated chitosan oligomers. Cytotoxicity data from wet lab experiments validated the model's predictive performance. The VQNN model demonstrated strong predictive capabilities in evaluating anticancer compounds. Specifically, it achieved a mean absolute error (MAE) of 6.5844, indicating a similar trend to the experimental results. Additionally, the model obtained an R2 value of 0.6020, reflecting a moderate level of correlation between predicted and observed outcomes. The results underscore the potential of integrating quantum-based machine learning models into cancer research. The VQNN effectively predicted experimental outcomes, showcasing its utility in assessing novel anticancer compounds. This approach could speed up drug discovery by streamlining the identification and optimization of therapeutic candidates. Furthermore, the findings support the ongoing development of quantum computing techniques for tackling complex biological challenges, contributing to innovative cancer treatment strategies that target tumor growth and angiogenesis.Öğe Single-Chain Variable Fragment-Based Dot Blot, Single, and Multiple Assays for Rapid SARS-CoV-2 Diagnostics(Oxford Univ Press Inc, 2025) Cam Derin, Dilek; Gultekin, Enes; Taskin, Irmak Icen; Dundar, Muhammed; Otlu, BarisBackground Antigenic detection is reliably utilized in rapid diagnostic tests and provides a significant time advantage during pandemics and epidemics. Therefore, the rapid detection of viral infections is of great importance and will remain crucial in the future. The SARS-CoV-2 outbreak, which resulted in severe losses, is the most recent example of this necessity. Among rapid diagnostic tests, lateral flow assays (LFAs) are the most practical and do not require specialized equipment, typically being developed using antibody pairs.Objective This study aimed to recombinantly produce a single-chain variable fragment (scFv) specific to the SARS-CoV-2 spike receptor-binding domain (sRBD) and to employ it in the development of LFAs utilizing both antibody and aptamer pairs and an aptamer cocktail.Methods Gold nanoparticles were employed as labeling agents, while both the scFv and full length forms of CR3022, along with aptamers specific to the S and N proteins, were utilized in a sandwich assay format.Results scFv was produced at a higher concentration and biologically active. It demonstrated effective viral detection in single LFA, dot blot assay (DBA), and multiplex LFA. While single LFA successfully detected only the synthetic target, DBA and multiplex LFA selectively identified the virus in nasopharyngeal and oropharyngeal swab samples.Conclusion Findings highlight the differences and effectiveness of using scFv in combination with other capture agents and different assay principles for the development of cost-effective and rapid diagnostic tests.Highlights scFvs exhibit variable binding in sandwich assays depending on the combinations employed. When used in combination with an aptamer cocktail, scFvs demonstrate enhanced target binding, which is shown for the first time in this study. The use of multiple testing strategies enables a more effective viral diagnosis.
