Yazar "Taslidere, Elif" seçeneğine göre listele
Listeleniyor 1 - 20 / 34
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe ACA, an inhibitor phospholipases A2 and transient receptor potential melastatin-2 channels, attenuates okadaic acid induced neurodegeneration in rats(Pergamon-Elsevier Science Ltd, 2017) Cakir, Murat; Duzova, Halil; Tekin, Suat; Taslidere, Elif; Kaya, Gul Busra; Cigremis, Yilmaz; Ozgocer, TubaAim: In recent studies, it has been shown that the Transient Receptor Potential Melastatin-2 Channels (TRPM2) and Phospholipases A2 (PLA(2)) inhibitors may have a protective effect on neurons. This study was aimed to investigate the protective effect of TRPM2 and PLA(2) inhibitor N-(p-amylcinnamoyl) Anthranilic Acid (ACA) in a neurodegenerative model induced by Okadaic Acid (OKA). Main methods: OKA (200 ng/10 mu l) was administered bilateral intracerebroventricularly as a single injection. Key findings: OKA-treated rats showed significant impairments of spatial memory in Morris Water Maze Test. OKA-induced memory-impaired rats showed increased numbers of degenerated neurons and Caspase-3, tau phosphorylated ser396, beta-amyloid positive cells in the hippocampus and cerebral cortex. Furthermore, OKA-treated rats exhibited significantly increased MDA, TNF-alpha levels, and decreased SOD, GSH-PX enzyme activates and GSH levels of the tissues. ACA administration ameliorated OKA-induced memory impairment in rats. The ACA treatment also increased SOD and GSH-PX enzyme activation and GSH levels, and conversely decreased the levels of MDA, TNF-alpha. It was found that the numbers of the degenerated neurons and Caspase-3 positive cells of cortex and hippocampus regions were significantly reduced. Significance: ACA administration attenuates the oxidative stress and neuroinflammation of OKA-induced neurodegeneration; and ameliorates the cognitive decline and neurodegeneration. (C) 2017 Elsevier Inc. All rights reserved.Öğe Ameliorative effects of aminoguanidine on rat aorta in Streptozotocin-induced diabetes and evaluation of ?-SMA expression(Turkish Soc Cardiology, 2014) Elbe, Hulya; Vardi, Nigar; Orman, Dogan; Taslidere, Elif; Yildiz, AzibeObjective: Diabetes mellitus is one of the chronic metabolic diseases which is characterized by microvascular and macrovascular complications. This study was designed to investigate the improving the effects of amnioguanidine on aortic damage in a streptozotocin (STZ) induced diabetic rat model. Methods: Thirty-two male Sprague-Dawley rats divided into four groups as follows: Control, Aminoguanidine, Diabetes, and Diabetes+Aminoguanidine. Experimental diabetes was induced by single dose STZ (45 mg/kg) intraperitoneally. After administration of STZ, the DM+AMG group began to receive AMG (1 g/L) was prepared by dissolving in tap water during 10 weeks. At the end of the study, blood glucose levels were determined and rats were sacrified by ketamine anesthesia. Following routine tissue process, aortas were embedded in paraffin. Histochemical (H-E and Orcein) and immunohistochemical alpha-smooth muscle actin (alpha-SMA) stains were applied and the sections examined by light microscope. Statistical analysis was carried out using the SPSS 13.0 statistical program. Results: The rats in diabetes group had significantly higher blood glucose levels than the rats of control. The main histological alterations were detected in tunica media such as extensive thickening (414.32 +/- 9.62 mu m), irregular of elastic fibers and intensive alpha-SMA staining in diabetic rats. The thickness of tunica media was statistically increased in DM group, when compared with the control group (p<0.001). On the other hand, AMG prevented disorganization of elastic fibers and overexpression of alpha-SMA. The mean thickness of tunica media was decreased significantly in DM+AMG (319.16 +/- 6.53 mu m) compared with the DM group (p<0.001). Conclusion: Our results demonstrate that AMG treatment may protect the impairment of aort structure at histological level.Öğe Aminoguanidine mitigates apoptosis, testicular seminiferous tubules damage, and oxidative stress in streptozotocin-induced diabetic rats(Churchill Livingstone, 2015) Orman, Dogan; Vardi, Nigar; Ates, Burhan; Taslidere, Elif; Elbe, HulyaThis study aimed to investigate the effect of aminoguanidine (AG) against testicular damage streptozotocin (STZ) induced diabetes. Thirty two rats were separated into four groups: control, AG, STZ and STZ + AG. In the STZ group, 12.5 +/- 1.3% of tubules were seen as containing sloughed spermatogenic cells into the lumen, 28.7 +/- 1.8% of tubules were atrophic, 46.2 +/- 2.1% of tubules were degenerative and 8.5 +/- 0.9% of tubules contained giant cells. Statistically, the affected tubule number was significantly lower in the STZ + AG group than in the STZ group. Intensely stained caspase-3 cells showed a statistically significant increase in the STZ group, while it decreased in the STZ + AG group. The enzyme activities of catalase (CAT), superoxide dismutase (SOD) and glutathione (GSH) level decreased and the level of malondialdehyde (MDA) and nitric oxide (NO) increased in the STZ group, while AG treated diabetic rats showed an increase of CAT, SOD activity and GSH level and a decrease in MDA and NO levels. This study shows that the oxidative stress, increased NO level and apoptotic cell death play an important role in diabetic rat testicular damage and that AG treatment of diabetic rats results in protection of spermatogenic cells against oxidative stress and apoptotic cell death. (C) 2015 Elsevier Ltd. All rights reserved.Öğe Anti-Apoptotic Effects of Aminoguanidine Against Liver Damage on Experimental Diabetes in Rats(İnönü Üniversitesi Tıp Fakültesi Dergisi, 2014) Taslidere, Elif; Vardi, Nigar; Orman, Dogan; Elbe, HulyaObjective: This study was designed to investigate the antiapoptotic and improving effects of aminoguanidine on the histological alterations in liver in streptozotocin (STZ)-induced diabetic rat model. Material and Methods: 32 male Sprague Dawley rats were divided into the following 4 groups: Control, Aminoguanidine (AMG), Streptozotocin (STZ), Streptozotocin + Aminoguanidine (STZ+AMG). The animals in group STZ and STZ+AMG were made diabetic by intraperitoneal injection of streptozotocin 45 mg/kg. Histochemical and immunohistochemical staining methods were applied to sections obtained from paraffin blocks and preparations were examined by using Leica DFC-280 light microscope. Results: The sections taken from the control and AMG groups were normal in histological appearance. However, multifocal nodules containing inflammatory cells were observed in the diabetic group. Moreover necrotic hepatocytes and hemorrhagic areas were observed among intact hepatocyte of lobul in the STZ group. Glycogen loss was observed in the hepatocytes localized at the periphery of lobule. In addition, the numbers of cells with positive staining by caspase-3 were significantly increased. On the other hand, it was seen that, in the groups administered with STZ, histological injury in the liver was attenuated by the administration of AMG. Moreover, it was found that the number of caspase-3 positive cells was significantly decreased in the STZ+AMG group. Conclusion: We concluded that chronic aminoguanidine administration reduced liver injury in STZ- induced diabetic rats. Thus, we suggest that aminoguanidine may be used to prevent the development of diabetic liver damage.Öğe Anti-inflammatory and antioxidative effects of dexpanthenol on nicotine-induced lung injury in rats(Korean Soc Environmental Risk Assessment & Health Science, 2023) Aslan, Meral; Guerel, Elif; Ueremis, Nuray; Ueremis, Muhammed Mehdi; Taslidere, ElifAim Lung inflammation is a consequence of smoking, tobacco use, nicotine addiction, and the accumulation of toxicants in the body. This study aimed to investigate the association between nicotine-induced lung injury and NF-kappa B activation, as well as changes in redox balance. Furthermore, the protective role of Dexpanthenol against this damage was examined. Method A total of 32 male rats were divided into four groups: Control, DEX, Nicotine, and Nicotine + DEX. Nicotine (0.5 mg/kg/day) and Dexpanthenol (500 mg/kg/day) were administered intraperitoneally. Subsequently, the levels of nuclear and cytoplasmic NF-kappa B in lung tissue were analyzed. Inflammation and oxidative stress markers and histopathological evaluations of the lung tissue were conducted. Results Nicotine administration resulted in increased levels of tissue MDA and TOS, as well as decreased levels of GSH-Px, GSH, GST, SOD, and TAS. Additionally, nicotine administration led to elevated nuclear expression of NF-kappa B protein, IL-1 beta, IL-6 proinflammatory cytokine levels, and Galectin-3 levels, which modulate cytokine release. Moreover, histopathological examinations revealed a higher population of diffuse lymphocytes and macrophages, indicating increased lung inflammation. Dexpanthenol administration ameliorated these adverse effects of nicotine and reduced them to levels comparable to the control group. Conclusion Nicotine-induced lung injury promoted oxidative stress and inflammation through modulation of NF-kappa B's nuclear translocation. Dexpanthenol, on the other hand, may serve as a dietary supplement to mitigate lung inflammation caused by smoking and tobacco use.Öğe Beta-glucan attenuates cerebral ischemia/reperfusion-induced neuronal injury in a C57BL/J6 mouse model(Univ Sao Paulo, Conjunto Quimicas, 2019) Kaya, Kursat; Ciftci, Osman; Oztanir, Mustafa Namik; Taslidere, Elif; Turkmen, Nese BasakBeta-glucans (beta g), that have many useful effects on human health, are natural polysaccharides. Our aim in this study was to determine useful effect of beta g against oxidative and neuronal damage caused by global cerebral ischemia/reperfusion (IR) in stroke imitated mice via surgical operation. A total of 40 mice divided into four equal groups randomly. The group 1 (sham operated) was kept as control. Bilateral carotid arteries of subjects in group 2 (I/R) and group 4 (I/R + beta g) were clipped for 15 min, and the mice in group 4 (I/R + beta g) were treated with beta g (50 mg/kg/day), while the mice in group 2 (I/R) were treated with only vehicle for 10 days. The mice of group 3 (beta g) were treated with beta g for 10 days without carotid occlusion. Global cerebral I/R significantly increased oxidative stress and decreased members of anti-oxidant defense system. In addition, I/R caused histopathological damage in the brain tissue. However, beta g treatment ameliorated both oxidative and histopathological effects of I/R. Our present study showed that beta g treatment significantly ameliorated oxidative and histological damage in the brain tissue caused by cerebral I/R. Therefore, beta g treatment can be used as supportive care for ischemic stroke patients.Öğe Biochemical and histopathological investigation of the protective effects of melatonin and vitamin E against the damage caused by acetamiprid in Balb-c mouse testicles at light and electron microscopic level(Springer Heidelberg, 2022) Zayman, Emrah; Gul, Mehmet; Erdemli, Mehmet Erman; Gul, Semir; Bag, Harika Gozukara; Taslidere, ElifThe protective effects of melatonin (Mel) and vitamin E (Vit E) against the negative effects of acetamiprid (Acmp) on testicles, reproductive hormones, and oxidative stress parameters were investigated in the present study. A total of 50 Balb-c male mice were used in 7 groups; 6 mice in the control groups (distilled water, corn oil, ethanol), and 8 in other groups (Acmp, Acmp + Mel, Acmp + Vit E, Acmp + Vit E + Mel). After the experiment, which lasted 21 days, hematoxylin eosin (H&E), periodic acid Schiff (PAS), and caspase-3 immunohistochemical (IHC) staining was performed on the testicular tissues. Also, the tissues were examined ultrastructurally with the transmission electron microscopy (TEM). In the Acmp group, there were decreased seminiferous tubule diameter and epithelial thickness, epithelial degeneration, decreased spermatozoa in the lumen, decreased PAS-positive staining in the seminiferous epithelial basement membrane, edema in the interstitial area, and hydropic degeneration in Leydig cells. Caspase-3 immunoreactivity was higher than in the other groups. TEM examination showed degeneration in tubule cells, lysosomal accumulation in cells of the spermatogenic line, vacuolizations with myelin figures, and necrosis. Hydropic degeneration, electron-dense lipid vacuoles, and chromatolysis were evident in the Leydig cell cytoplasm. In Sertoli cells, electron-dense lysosomal deposits were noted. In biochemical terms, there were decreased tissue glutathione (GSH) and total antioxidant status (TAS), and increased malondialdehyde (MDA) and total oxidant status (TOS). Plasma luteinizing hormone (LH), follicular stimulating hormone (FSH), and testosterone levels were decreased. In the groups with melatonin, vitamin E, and both were applied together, tissue damage, and apoptotic cell death were reduced at both light microscopic and ultrastructural levels. In biochemical terms, there were decreased oxidative parameters and increased hormonal parameters. It was found that vitamin E was more effective in decreasing oxidative parameters and increasing antioxidative parameters when compared to melatonin, and hormonal parameters increased at a higher level in the Acmp + Vit E group than in all groups. As a result, it was found that exposure to Acmp caused damage to testicular tissue, induced oxidative stress in testicles, and decreased plasma LH, FSH, and testosterone levels, and although vitamin E is more effective than melatonin in preventing this damage, both are effective.Öğe Covid-19 and Sars: Two corona virus pandemics encountered in the last two decades(2020) Taslidere, Elif; Vardi, Nigar; Cirik, Hilal RumeyzaThe New Corona Virus Disease declared by the World Health Organization as pandemics on 11th March 2020, is the third pandemics caused by coronaviruses since the beginning of the 21st century. Other pandemics are Severe Acute Respiratory Syndrome and Middle East Respiratory Syndrome. While the similarity between SARS-CoV-2 and MERS-CoV was 50%, the same between SARS-CoV-2 and SARS-CoV was higher than 80%. With regard to virus classification, those three viruses, causing pandemics belonged to the coronavirus (in Latin: Orthocoronavirinae) sub-family of the Coronaviridae family. The Corona Viruses are enveloped RNA viruses which cause a wide spectrum of respiratory tract infections in human, other mammals or birds. The receptor bound by SARS-CoV and SARS-CoV-2 to penetrate to the host cell is common, namely angiotensin converter enzyme 2 receptor. Corona viruses are shown to bind to the angiotensin converter enzyme 2 receptor on the surface of human cell by means of the spike protein (S protein) being one of the structural proteins existing on its outer envelope. As SARS-CoV-2 is a newly appearing virus, in this compilation we compared the two coronavirus pandemics, i.e. Severe Acute Respiratory Syndrome and the New Corona Virus Disease, encountered in the last two decades. We searched the function of the angiotensin converter enzyme 2 in cells, in which the angiotensin converter enzyme 2 is expressed, similarities and differences of the clinical profile of SARS and COVID-19. Our purpose is to compile the information and experience obtained from the Severe Acute Respiratory Syndrome outbreak with the understanding we gained from the New Corona Virus Disease and to assist in offering new ideas in an effort to prevent this pandemic.Öğe Design of Xylose-Based Semisynthetic Polyurethane Tissue Adhesives with Enhanced Bioactivity Properties(Amer Chemical Soc, 2016) Balcioglu, Sevgi; Parlakpinar, Hakan; Vardi, Nigar; Denkbas, Emir Baki; Karaaslan, Merve Goksin; Gulgen, Selam; Taslidere, ElifDeveloping biocompatible tissue adhesives with high adhesion properties is a highly desired goal of the tissue engineering due to adverse effects of the sutures. Therefore, our work involves synthesis, characterization, adhesion properties, protein adsorption, in vitro biodegradation, in vitro and in vivo biocompatibility properties of xylose-based semisynthetic polyurethane (NPU-PEG-X) bioadhesives. Xylose-based semisynthetic polyurethanes were developed by the reaction among 4,4'-methylenebis(cyclohexyl isocyanate) (MCI), xylose and polyethylene glycol 200 (PEG). Synthesized polyurethanes (PUs) showed good thermal stability and high adhesion strength. The highest values in adhesion strength were measured as 415.0 +/- 48.8 and 94.0 +/- 2.8 kPa for aluminum substrate and muscle tissue in 15% xylose containing PUs (NPU-PEG-X-15%), respectively. The biodegradation of NPU-PEG-X-15% was also determined as 19.96 +/- 1.04% after 8 weeks of incubation. Relative cell viability of xylose containing PU was above 86%. Moreover, 10% xylose containing NPU-PEG-X (NPU-PEG-X-10%) sample has favorable tissue response, and inflammatory reaction between 1 and 6 weeks implantation period. With high adhesiveness and biocompatibility properties, NPU-PEG-X can be used in the medical field as supporting materials for preventing the fluid leakage after abdominal surgery or wound closure.Öğe Development of Liver and Pancreas(Bezmialem Vakif Univ, 2017) Esrefoglu, Mukaddes; Taslidere, Elif; Cetin, AsliThe parenchyma of the liver and pancreas is derived from the endoderm, whereas the stroma is derived from the mesoderm. Both of them are derived from the endoderm of the foregut as the esophagus, stomach, and a part of duodenum. At the 3rd-4th of development, the liver, gallbladder and bile ducts become diverticulum hepaticum that is derived from the caudal portion of the foregut. There were inductive effects of septum transversum and cardiac mesoderm for the development of liver diverticulum. The pancreas arise from the endoderm of the foregut. The pancreas is derived from the fusion of the ventral and dorsal pancreas bulbs, which arise from the endoderm of the duodenum. The inductive effects of the notochord and dorsal aorta play a role in the development of the pancreas. In this manuscript, we attempted to review the morphological and functional development of the liver and pancreas with the aid of pictures obtained from various stages of prenatal and postnatal development in the organs of rats.Öğe Development of the Esophagus and Stomach(Aves, 2017) Esrefoglu, Mukaddes; Taslidere, Elif; Cetin, AsliEpithelial components of the organs of the digestive system are derived from the endoderm, whereas connective tissue and muscle components are derived from the mesoderm. At the 3rd-4th week of development, as a result of cephalocaudal and lateral foldings of the embryo, a portion of the endoderm-lined yolk sac cavity is incorporated in the embryo to form the primitive gut. Primitive gut is composed of four main regions: pharyngeal gut, foregut, midgut, and hindgut. The esophagus and stomach are derived from the foregut. The development of the esophagus is characterized by lengthening, widening, thickening, and histological changes. The development of the stomach is characterized by widening, thickening, and histological changes as well as positional changes. In the present study, we tried to review the morphological and functional development of the esophagus and stomach with the aid of pictures obtained from various stages of prenatal and postnatal development of the organs of rats. Previous reviews lack information on both histological and functional development of the organs.Öğe Dexpanthenol exhibits antiapoptotic and anti-inflammatory effects against nicotine-induced liver damage by modulating Bax/Bcl-xL, Caspase-3/9, and Akt/NF-?B pathways(Wiley, 2024) Uremis, Nuray; Aslan, Meral; Taslidere, Elif; Gurel, ElifChronic tobacco use can lead to liver damage and inflammation due to the accumulation of various toxins in the body. This study aimed to investigate the correlation between the molecular mechanisms of nicotine-induced liver injury, the caspase cascade, and the Akt/NF-kappa B signaling pathway, as well as the protective effects of dexpanthenol (DEX). Male rats were subjected to intraperitoneal injections of nicotine at a concentration of 0.5 mg/kg/day and/or DEX at a concentration of 500 mg/kg/day for 8 weeks. After the treatment period, liver function tests were conducted on serum samples, and tissue samples were analyzed for protein levels of Akt, NF-kappa B, Bax, Bcl-xL, Caspase-3, and Caspase-9, along with histopathological changes. Additionally, assessments of oxidative stress markers and proinflammatory cytokines were carried out. Nicotine administration led to elevated levels of IL-6, IL-1 beta, MDA, TOS, and oxidative stress index, accompanied by decreased TAS levels. Moreover, nicotine exposure reduced the p-Akt/Akt ratio, increased NF-kappa B, Bax, Caspase-3, and Caspase-9 protein levels, and decreased the antiapoptotic protein Bcl-xL levels. DEX treatment significantly mitigated these effects, restoring the parameters to levels comparable to those of the control group. Nicotine-induced liver injury resulted in oxidative stress, inflammation, and apoptosis, mediated by Bax/Bcl-xL, Caspase-3, Caspase-9, and Akt/NF-kappa B pathways. Conversely, DEX effectively attenuated nicotine-induced liver injury by modulating apoptosis through NF-kappa B, Caspase-3, Caspase-9, Bax inhibition, and Bcl-xL activation. Chronic nicotine exposure led to oxidative stress, inflammation, and apoptosis, as evidenced by altered protein levels in Bax/Bcl-xL, Caspase-3/9, and Akt/NF-kappa B pathways. Dexpanthenol treatment effectively countered these effects, highlighting its potential as a therapeutic agent for nicotine-related liver injury.imageÖğe Dexpanthenol protects against nicotine-induced kidney injury by reducing oxidative stress and apoptosis through activation of the AKT/Nrf2/HO-1 pathway(Springer, 2024) Uremis, Muhammed Mehdi; Gurel, Elif; Aslan, Meral; Taslidere, ElifDexpanthenol (DEX), a subtype of vitamin B5, plays an important role in anabolic reactions, cellular energy and regeneration in the body. Nicotine has been shown to induce kidney damage through the mechanisms of oxidative stress and apoptosis. The purpose of this study was to investigate the potential protective effects of DEX against nicotine-induced kidney damage through modulation of the AKT/Nrf2/HO-1 signaling pathway. Male rats were intraperitoneally administered with 0.5 mg/kg/day nicotine and/or 500 mg/kg/day DEX for 8 weeks. Following administration, renal function tests were conducted on serum samples, and histopathological examinations and analysis of oxidative stress markers and antioxidant enzymes were performed on tissue samples. Protein levels of Akt, Nrf-2, HO-1, Bcl-xL, and Caspase-9 were also evaluated. Nicotine administration resulted in decreased protein levels of p-Akt, Nrf-2, HO-1, and Bcl-xL and increased Caspase-9 protein levels. In addition, nicotine administration caused an increase in MDA, TOS, and OSI levels and a decrease in GSH, GSH-Px, GST, CAT, SOD, and TAS levels. Additionally, BUN and Creatinine levels increased after nicotine administration. DEX administration positively regulated these parameters and brought them closer to control levels. Nicotine-induced kidney injury caused apoptosis and oxidative stress through Caspase-9 activation. DEX effectively prevented nicotine-induced kidney damage by increasing intracellular antioxidant levels and regulating apoptosis through Bcl-xL activation. These findings suggest that DEX has potential as a protective agent against nicotine-induced kidney damage.Öğe Dexpanthenol therapy reduces lung damage in a hyperoxic lung injury in neonatal rats(Taylor & Francis Ltd, 2016) Ozdemir, Ramazan; Demirtas, Gulsum; Parlakpinar, Hakan; Polat, Alaadin; Tanbag, Kevser; Taslidere, Elif; Karadag, AhmetObjective: Dexpanthenol (Dxp) plays a major role in cellular defense and in repair systems against oxidative stress and inflammatory response and it has not yet been evaluated in treatment of bronchopulmonary dysplasia (BPD). We tested the hypothesis that proposes whether Dxp decreases the severity of lung injury in an animal model of BPD.Methods: Forty rat pups were divided into four groups: control, control+Dxp, hyperoxia and hyperoxia+Dxp. All animals were processed for lung histology and tissue analysis. The degree of lung inflammation, oxidative and antioxidant capacity was assessed from lung homogenates.Results: Lung injury score and alveol diameter increased in the hyperoxia group (p<0.001). Median level of malondialdehyde, total oxidant status and oxidative stress indexes was significantly higher in the hyperoxia group compared to the other groups. The median superoxide dismutase activity in the hyperoxia group was notably less than those of control+Dxp and hyperoxia+Dxp groups (p<0.01). Similarly, lung catalase, glutathione (GSH) peroxidase and reduced GSH activities in the hyperoxia group were significantly lower than other groups. Furthermore, the hyperoxia+Dxp group had lower tumor necrosis factor- and interleukin-1 median levels compared to the hyperoxia group (p=0.007).Conclusion: Dxp treatment results in less emphysematous change as well as decrease in inflammation and oxidative stress markers in an animal model of BPD.Öğe The Effect of Ceftriaxone on Testicular Connexin 43 Expression.(Sage Publications Inc, 2016) Sahin, Levent; Sahin, Hilal; Vardi, Nigar; Karahan, Feride; Yildiz, Azibe; Taslidere, Elif; Gul, Semir[Abstract Not Available]Öğe The effect of dexmedetomidine against oxidative and tubular damage induced by renal ischemia reperfusion in rats(Taylor & Francis Ltd, 2015) Cakir, Murat; Polat, Alaadin; Tekin, Suat; Vardi, Nigar; Taslidere, Elif; Duran, Zeynep Rumeysa; Tanbek, KevserDexmedetomidine (dex) is a potent, highly selective and specific alpha 2-adrenoreceptor agonist. This experimental study was designed to investigate protective and therapeutic effect of two different doses of dex, on kidney damage induced by ischemia-reperfusion (I/R) in rats. Male Sprague - Dawley rats were divided into four groups, each including 10 animals: control group, ischemia-reperfusion (I/R) group; treated groups with 10 mu g/kg of dex and 100 mu g/kg of dex. After removing right kidney of the rats, the left kidney has performed ischemia during 40 min and reperfusion in the following 3 h. The histopathological findings, and also tissue superoxide dismutase (SOD) and catalase (CAT) enzyme activity, malondialdehyde (MDA), glutathione (GSH), serum blood urea nitrogen (BUN), creatinine (Cre) and tumor necrosis factor-alpha (TNF-alpha) levels were determined. In the I/R group, compared to the control group, levels of BUN, Cre and kidney tissue MDA have increased significantly, SOD, CAT enzyme activity and glutathione levels have decreased significantly. In the dex10 group, compared to the I/R group, levels of Cre and TNF-alpha have decreased significantly, while the SOD activity has increased significantly. In the dex100 group, compared to the I/R group, levels of BUN, Cre have decreased significantly, while the SOD activity has increased significantly. In the I/R group, there was also extensive tubular necrosis, glomerular damage in the histological evaluation. Dex ameliorated these histological damages in different amounts in two treatment groups. In this study, the protective effects of dex against renal I/R injury have been evaluated by two different amount of doses.Öğe Effects of Ozone Therapy on Cyclophosphamide-induced Urinary Bladder Toxicity in Rats(Canadian Soc Clinical Investigation, 2013) Tasdemir, Seda; Tasdemir, Cemal; Vardi, Nigar; Ates, Burhan; Taslidere, Elif; Karaaslan, Merve G.; Sapmaz, Hilal I.Purpose: This study investigated the efficacy of ozone therapy (OT) in a rat model of cyclophosphamide-induced hemorrhagic cystitis (HC). Methods: Forty Wistar Albino male rats were divided into five groups: sham, OT, cyclophosphamide (CP), OT+CP and CP+OT. Hemorrhagic cystitis (HC) was induced by intraperitoneal (i.p) administration a single dose of 100 mg/kg CP. OT was performed once daily for three days. the CP+OT group received OT (0.2 mg/kg) i.p 24 h after CP administration. CP was injected to the OT+CP group the day after the third course of OT. All animals were killed four days after CP administration. Bladder injury and oxidative stress parameters were determined from tissue samples. Results: We found small, but non-statistically significant biochemical and histological changes in the animals treated with OT alone. CP administration induced cystitis, as manifested by a marked loss of urothelial cells, as well as hemorrhaging and edema in the bladder as determined by histopathological examination. It also caused a significant decrease in the endogenous antioxidant compound glutathione (GSH) and elevation of lipid peroxidation, and nitric oxide (NO) and myeloperoxidase (MPO) levels in the rats' urinary bladder tissue. OT was able to ameliorate these changes; however these effects were prominent in the CP+OT group when compared with the OT+CP group.: For example, the NO level in the CP+OT group was 68% of the OT+CP group (p < 0.05). Conclusion: OT prevented CP-induced urothelial damage by diminishing bladder oxidative stress, inflammation and NO levels. OT may help to ameliorate bladder damage induced by CP in the clinical setting.Öğe The effects of pentoxifylline and caffeic acid phenethyl ester on TNF-? and lung histopathology in D-galactosamine-induced pulmonary injury in rats(Churchill Livingstone, 2023) Taslidere, Elif; Vardi, Nigar; Yildiz, Azibe; Ates, Burhan; Esrefoglu, MukaddesIn this study, we aimed to investigate the effects of pentoxifylline [PTX] and caffeic acid phenethyl ester [CAPE] in D-galactosamine [D-GAL]-induced pulmonary injury in rats. The rats were randomly divided into six groups: control, D-GAL, D-GAL+PTX, D-GAL+CAPE, PTX and CAPE. Each group included eight animals. Lung sections from the control, PTX and CAPE groups had a normal histological appearance. The D-GAL group showed histopathological changes in lung tissue, including haemorrhage, oedema, inter-alveolar septal thickening and widespread infiltration of inflammatory lymphocytes and macrophages. Administration of PTX and CAPE significantly reduced histopathological damage scores in the D-GAL+PTX and D-GAL+CAPE groups compared with the D-GAL group. PTX and CAPE treatment also significantly decreased malondialdehyde levels, increased levels of reduced GSH and increased catalase and superoxide dismutase activity in lung tissue samples. These results indicate that the destructive effects of D-GAL-induced inflammation in the rat lung are significantly reduced following administration of PTX and CAPE.Öğe Effects of Quercetin on Cisplatin-Induced Renal Damage in Wistar Albino Rats(Galenos Publ House, 2022) Cetinavci, Dilan; Elbe, Hulya; Taslidere, Elif; Bostancieri, Nuray; Taslidere, AsliAim: Cisplatin is one of the effective antineoplastic drugs widely used in the treatment of many types of cancer. Cisplatin has harmful effects such as nephrotoxicity, ototoxicity and cardiomyopathy. Quercetin is an antioxidant of the flavonoid group. In this study, it was aimed to investigate the therapeutic effects of quercetin against cisplatin-induced kidney damage in rats. Materials and Methods: Twenty-eight male Wistar albino rats were randomly selected and divided into 4 groups: Group 1: Control (no application), Group 2: Quercetin (25 mg/kg/7 days/intraperitoneal), Group 3: Cisplatin (7 mg/kg/single dose/ intraperitoneal), Group 4: Cisplatin+quercetin (7 mg) /kg/single dose/ intraperitoneal cisplatin followed by 25 mg/kg/7 days/ intraperitoneal quercetin). After routine histological follow-up, hematoxylin eosin and periodic acid-schiff staining were performed. Histopathological damage score was calculated. Caspase-3 immunostaining was performed and scored. Results: Control and quercetin groups had normal histological appearance. In the cisplatin group, dilatation of the tubules, epithelial shedding, vacuolization of the tubular epithelial cells, and loss of microvilli in the proximal tubules were detected. In addition, infiltration areas were also found in places. In addition, an increase in caspase-3 immunostaining intensity was detected in this group (p=0.000). Histopathological findings were significantly reduced in the cisplatin+quercetin group compared to the cisplatin group (p=0.001). Conclusion: In this study, we think that quercetin is histopathologically beneficial in the treatment of cisplatin-induced kidney damage.Öğe Effects of the apricot diets containing sulfur dioxide at different concentrations on rat testicles(Springer Heidelberg, 2023) Yildiz, Azibe; Ozhan, Onural; Ulu, Ahmet; Dogan, Tugba; Bakar, Busra; Ugur, Yilmaz; Taslidere, ElifDue to its antioxidant and antimicrobial properties, sulfur dioxide (SO2) is widely used in foods and beverages to prevent the growth of microorganisms and to preserve the color and flavor of fruits. However, the amount of SO2 used in fruit preservation should be limited due to its possible adverse effects on human health. The present study was designed to investigate the effects of different SO2 concentrations in apricot diets on rat testes. Animals were randomly divided into six groups. The control group was fed a standard diet, and the other groups were fed apricot diet pellets prepared with (w/w) 10% dried apricots containing SO2 at different concentrations (1500 ppm, 2000 ppm, 2500 ppm, 3000 ppm, and 3500 ppm/kg) for 24 weeks. After sacrification, testicles were evaluated biochemically, histopathologically, and immunohistopathologically. Our results showed that an apricot diet containing 1500 ppm and 2000 ppm SO2 did not cause significant changes in testis. However, it was determined that tissue testosterone levels decreased as the amount of SO2 (2500 ppm and above) increased. Apricot diet containing 3500 ppm SO2 caused a significant increase in spermatogenic cell apoptosis, oxidative damage, and histopathological changes. In addition, a decrease in the expression of connexin-43, vimentin, and 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) was observed in the same group. In summary, the results show that sulfurization of apricot at high concentrations such as 3500 ppm may lead to male fertility problems in the long term through mechanisms such as oxidative stress, spermatogenic cell apoptosis, and inhibition of steroidogenesis.
