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Öğe Acute central nervous system depression after subcutaneous use of prilocaine in an infant(2012) Karabiber H.; Selimoglu M.A.; Cetin M.; Tekin S.; Gurbuz S.[No abstract available]Öğe Asprosin improved neuronal survival by suppressing apoptosis and enhancing the activity of the autophagy pathway in the MCAO model in rats(Verduci Editore s.r.l, 2024) Tanbek K.; Yuksel F.; Tekin S.; Tekin C.; Sandal S.OBJECTIVE: Cerebral ischemia (CI) is a condition in which metabolic stress increases when blood flow is interrupted in a part of the brain, resulting in oxygen and glucose deprivation. It is known that asprosin (Asp), secreted from adipose tissue during fasting, has an effect on some metabolic processes such as apoptosis, autophagy, and glucose metabolism. This study aimed to explain which of the cell death/survival Asp induces in the CI/reperfusion model. MATERIALS AND METHODS: In the study, 48 male Wistar Albino rats were divided into 6 groups: Sham, CI, Asp+CI, CI+Asp, CI+Asp+3-MA, and Asp+CI+3-MA (n=48). CI was created using the intraluminal filament technique for 60 minutes, autophagy inhibitor 3-MA (15 mg/kg/ day) and Asp (1 µg/kg/day) injections were administered 3 days before or 3 days during reperfusion. Beclin-1, ATG5, ATG7, p62, Bcl-2, Bax, active-caspase-3, and active-caspase-9 protein levels from brain tissues were determined by the Western-Blot method. The infarct area was determined by triphenyl tetrazolium chloride (TTC) staining. The Kruskal-Wallis’ test was used to compare differences between groups. p<0.05 was considered statistically significant. RESULTS: Compared to the Sham group, the increase in ischemic area and the decrease in Beclin-1, ATG-5, ATG-7, Bcl-2, Bax, active-caspase-3 and active-caspase-9 levels in the CI groups are statistically significant (p<0.05). The increase of Beclin-1, ATG-7, Bcl-2, and Bax levels in the Asp groups is statistically significant compared to the CI group (p<0.05). When Asp+CI groups and CI+Asp groups are compared, an increase in Beclin-1 levels in the Asp+CI group and the increase in Bcl-2, Bax, active-caspase-3/9 and ATG-5 levels in the CI+Asp groups are statistically significant (p<0.05). CONCLUSIONS: Asp has protective and therapeutic effects against CI/R damage. While applying Asp before ischemia activates the autophagy pathway more, applying it after ischemia protects the neuronal death/survival balance by activating the apoptosis pathway more. © 2024 Verduci Editore s.r.l. All rights reserved.Öğe (E)-1-(4-Hydroxyphenyl)-3-(substituted-phenyl) prop-2-en-1-ones: Synthesis, in Vitro Cytotoxic Activity and Molecular Docking Studies(Slovensko Kemijsko Drustvo, 2022) Sirka L.; Doğan H.; Bahar M.R.; Çalışkan E.; Tekin S.; Uslu H.; Koran K.A series of chalcone compounds (2–11) were designed and synthesized to determine their cytotoxic effects. The structures of 2–11 were fully characterized by their physical and spectral data. The in vitro cytotoxic effects of 2–11 were evaluated against human ovarian cancer (A2780), breast cancer (MCF-7) and prostate cancer (PC-3 and LNCaP) cell lines. The activity potentials of compounds were further evaluated through molecular docking studies with AutoDock4 and Vina softwares. All the compounds (except compound 5) showed significant cytotoxic effects at high doses in all cancer cell lines. Among all the compounds studied, one compound i.e. compound 2 demonstrated dose-dependent activity, particularly against A2780/LNCaP cancer cell lines. The most effective compounds 8, 9, 10 and 11 reduced the cell viability of A2780, MCF-7, PC-3 and LNCaP cells by 50–98%, while other compounds 2, 4 and 7 reduced the cell viability of A2780 cells by 70–90% at concentrations of 50 and 100 µM. © 2022 Slovensko Kemijsko Drustvo. All rights reserved.Öğe Effects of Perineural Administration of Dexmedetomidine in Combination with Levobupivacaine in a Rat Sciatic Nerve Block(2013) Ali Erdogan M.; Polat A.; Yucel A.; Aydogan M.S.; Parlakpinar H.; Tekin S.; Durmus M.Objective: The aim of this study was to assess if perineural administration of dexmedetomidine combined with levobupivacaine increases the duration of the sensory and motor blockade of a sciatic peripheral nerve block in rats. Methods: Forty male Sprague-Dawley rats were randomly divided into 5 experimental groups: Group 1, sham; Group 2, perineural levobupivacaine (0.2 mL of a 0.5% solution) and subcutaneous saline; Group 3, perineural levobupivacaine (0.2 mL of a 0.5% solution) plus dexmedetomidine (20 ?g/kg dexmedetomidine) and subcutaneous saline; Group 4, perineural saline and subcutaneous dexmedetomidine; and Group 5, perineural saline and subcutaneous saline. Pain reflexes in response to a thermal stimulus were measured at 0 and 240 minutes after drug administration by using a hot-plate and tail-flick tests. Neurobehavioral status, including sensory and motor functions, was assessed by an investigator who was blinded to the experimental groups every 30 minutes until normal functioning resumed. Results: The sensory and motor blockades of the rats did not increase in the treatment with dexmedetomidine plus levobupivacaine when compared with the treatment with levobupivacaine alone at all the time points ( P > 0.05). Compared with rats in Group 2, those in Group 3 showed significantly higher latency times at 30 and 60 minutes in the hot plate test ( P < 0.01). At 30 and 60 minutes, the latency times of the rats in Group 3 were longer than those in Group 2 in the tail-flick test ( P < 0.01). Furthermore, the durations of the complete sensory and motor blockade were similar when treatment with levobupivacaine plus dexmedetomidine was compared with treatment with levobupivacaine alone. Conclusions: A 20?g/kg dose of dexmedetomidine added to levobupivacaine did not increase the duration of the sensory and motor blockades in rats. However, treatment with dexmedetomidine plus levobupivacaine increased the quality of analgesia in rats. © 2013 The Authors.Öğe Phytochemical Composition, Antiradical, Antiproliferative and Antimicrobial Activities of Capsicum frutescens L.(Taylor and Francis Ltd., 2018) Keser S.; Kaygili O.; Keser F.; Tekin S.; Yilmaz Ö.; Demir E.; Kirbag S.The pepper fruits keep very important place in the human nutrition and contain some important components in terms of human health. Capsicum frutescens can be consumed both fresh and dry, and has been used in made powdered spices and sauces. In this study, the antiradical activities, phytochemical contents, antimicrobial, antiproliferative activities of water and ethanol extract of C. frutescens fruits were investigated. According to our study results, it was found that the ABTS and OH scavenging activities of C. frutescens extracts are better than the standard antioxidant BHT. It was established that C. frutescens contains high amount of ?-tocopherol, vitamin D, phytosterols, flavonoids, proanthocyanidin, phenolics and unsaturated fatty acids. Also, C. frutescens exhibit very high cytotoxic effects on the MCF-7, HCT-116, LNCaP cancer cell lines. Consequently, these results indicate that cayenne pepper may continue to be consumed by humans owing to it has potentially antioxidant, antiproliferative properties and including bioactive compounds. © 2018 Har Krishan Bhalla & Sons.Öğe Phytochemical Compounds and Biological Activities of Celtis tournefortii Fruits(Taylor and Francis Ltd., 2017) Keser S.; Keser F.; Kaygili O.; Tekin S.; Turkoglu I.; Demir E.; Turkoglu S.It is known that Celtis species have been used in healing wound, epilepsy crisis, and stomach pain among the people. The antiradical (DPPH, OH, ABTS) activities, phytochemical compounds (total proanthocyanidin, total flavonoid, total phenolic, flavonoids, phenolic acids, sterols, lipid soluble vitamins, fatty acids), antimicrobial, antiproliferative (MCF-7, HCT-116, A2780, PC-3) activities of water, ethanol, methanol extracts of C. tournefortii fruits were evaluated in this study. All the C. tournefortii fruit water extracts were higher scavenged OH radical than standard antioxidant BHT. It was detected that C. tournefortii includes high amount of ?-tocopherol (2.65 mg/kg), vitamin D (10.05 mg/kg), ergosterol (78.70 mg/kg), stigmasterol (185.90 mg/kg), unsaturated fatty acids (74.47%). C. tournefortii extracts showed antiproliferative activity on MCF-7, HCT-116, A2780, PC-3 cancer cell lines and antimicrobial activity on some microorganisms when compared to standard antibiotics. Also, this study is the first report about phytochemical contents, antiproliferative, antimicrobial, antiradical properties of C. tournefortii fruits. © 2017 Har Krishan Bhalla & Sons.
