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    The effect of oral puerarin administration on wound healing in diabetic rat model
    (2018) Turer, Akif; Onger, Mehmet Emin
    Aim: The aim of this study is to examined the effect of the oral administration of puerarin on wound healing in diabetic rat. Material and methods: Twenty-four rats were divided into three groups: Group 1 (n:8) non diabetic control (NDC), group 2 (n:8) diabetic control (DC), group 3 (n:8) diabetic puerarine (DP). Rats Group DC and DP were injected a single dose 200 mg/kg streptozin solution for diabet model. A full thickness linear incision was made in the paravertebral region of the rats and the wound edges were covered with a sterile plaster. In Group NDC and DC, rats were given saline solution for 7 days. In Group DP, rats were given 200 mg/ kg puerarin with oral gavage for 7 days. All animals were euthanized at 7 days postoperative. Connective tissue volumes and new vessel formation were measured with stereological analysis Results: Stereologic analysis showed that Group NDC and DP had significantly more connective tissue volume at 7 days compared with group DC. New vessel formation volumes were also significantly lesser in group DC. Connective tissue and new vessel volumes difference were not statistically significant between group NDC and DP. Conclusion: Orally administered puerarin enhances new vessel formation and support wound healing in diabetic rats.
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    Effects of single high-dose systemic vitamin D injection to dentin-grafted bone defects in osteoporotic rats
    (2019) Mercan, Ugur; Turer, Akif
    Aim: Osteoporosis is a metabolic bone disease posing a public health issue worldwide. Studies showed that dentine can be an alternative bone graft material in bone defects. The purpose of this study is to investigate the potential of the intraperitoneal administration of vitamin D on dentine grafted critical-sized cortical bone defects in osteoporotic rat model. Material and Methods: Twenty-four rats were underwent bilateral ovariectomy and 6 weeks after ovariectomy osteoporotic rats model were obtained and divided into three groups: Group A; Dentine-D vit, Group B; Dentine and Group C (control). A 5-mm diameter critical- size defect was created in the calvarium of each animal. In Group C, untreated control defects. In Group Dentin, defects were filled with particulate human dentine. In Group Dentin-D vit, defects were filled with particulate human dentine and rats were injected intraperitoneally a single high dose (50.000 U.I./kg) vitamin D. All animals were euthanized at 28 days postoperative. Results: New bone formation was evaluated by histologic and immunohistochemical analysis. Histologic analysis showed that Group Dentin-D vit and Dentin had significantly more new bone at 4 weeks compared with group C. According to immunohistochemical analyses, in group Dentin-D vit, BMP2 staining areas and density were statistically higher than Control group. TGF-β level differences between groups Dentin-D vit and Dentin were statistically significant. Group C had a significantly lower TGF-β grade than the other groups. The osteopontin staining areas in group Dentin-D vit were statistically higher grade than in group Dentin and group C. The difference between groups Dentin and C was also statistically significant. Conclusion: Intraperitoneally injected single high dose vitamin D had positive effect on dentine graft and bone formation.
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    Protective effects of puerarin on the periodontium in an experimental rat model of periodontitis with and without diabetes mellitus: A stereological and immunohistochemical study
    (2018) Coskun Turer, Cigdem; Turer, Akif; Altun, Gamze
    Aim: Periodontitis is a chronic inflammatory disease characterized by tissue destruction. Patients with diabetes mellitus are more susceptible to severe tissue destruction. Puerarin, a biological component derived from Pueraria lobate, has anti-inflammatory and anti-diabetic effects. The purpose of the study is to evaluate the protective role of puerarin on alveolar bone loss and connective tissue destruction in ligature induced diabetic and non-diabetic rats. Material and methods: Sixty rats were divided as non-diabetic control, non-diabetic-experimental periodontitis (EP)-systemic saline, non-diabetic-EP-systemic puerarin, diabetic control, diabetic-EP-systemic saline and diabetic-EP-systemic puerarin. Diabetes was induced by injection of streptozocin (200 mg/kg). EP was achieved by placing a sterile silk suture around the first molars into the gingival sulcus for 15 days. In puerarin treated groups, 200 mg/kg puerarin was applied daily for 15 days beginning one-day prior the placement of the ligature. The alveolar bone level of the first molar tooth, alveolar bone ratio in the furcation area and the attachment level were evaluated histologically. MMP-9, TIMP-1 levels and RANKL/OPG ratio were evaluated immunohistochemically. Results: Significantreduced alveolar bone and attachment losses were found in puerarin-treated groups comparing to in salineadministered groups (p<0.05). There was a significant reduction of RANKL/OPG ratio and MMP-9 levels and an increase in TIMP-1 levels in puerarin-treated groups compared with saline-administered groups during the induction of experimental periodontitis in both diabetic and non-diabetic condition (p<0.05). Conclusion: Puerarin might help to prevent alveolar bone loss and connective tissue destruction in periodontal disease in diabetic and non-diabetic condition.