Yazar "Tuzcu, Mehmet" seçeneğine göre listele
Listeleniyor 1 - 20 / 27
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe (3R, 3'R)-zeaxanthin protects the retina from photo-oxidative damage via modulating the inflammation and visual health molecular markers(Taylor & Francis Ltd, 2019) Sahin, Kazim; Akdemir, Fatih; Orhan, Cemal; Tuzcu, Mehmet; Gencoglu, Hasan; Sahin, Nurhan; Ozercan, Ibrahim H.Purpose: Zeaxanthin protects the macula from ocular damage due to light or radiation by scavenging harmful reactive oxygen species. In the present study, zeaxanthin product (OmniXan (R); OMX), derived from paprika pods (Capsicum annum; Family-Solanaceae), was tested for its efficacy in the rat retina against photooxidation. Methods: Forty-two male 8-week-old Wistar rats exposed to 12L/12D, 16L/8D and 24L/0D hours of intense light conditions were orally administrated either 0 or 100 mg/kg BW of zeaxanthin concentration. Retinal morphology was analyzed by histopathology, and target gene expressions were detected with real-time polymerase chain reaction methods. Results: OMX treatment significantly increased the serum zeaxanthin concentration (p < 0.001) and ameliorated oxidative damage by increasing the antioxidant enzyme activities in the retina induced by light (p < 0.001). OMX administration significantly upregulated the expression of genes, including Rhodopsin (Rho), Rod arrestin (SAG), G alpha Transducin 1 (GNAT-1), neural cell adhesion molecule (NCAM), growth-associated protein 43 (GAP43), nuclear factor-(erythroid-derived 2)-like 2 (Nrf2) and heme oxygenase (HO-1) and decreased the expression of nuclear factor-kappa B (NF- kappa B) and GFAP by OMX treatment rats. The histologic findings confirmed the antioxidant and gene expression data. Conclusions: This study suggests that OMX is a potent substance that can be used to protect photoreceptor cell degeneration in the retina exposed to intense light.Öğe 3R, 3?R zeaxanthin Protects Retina from Photo-Oxidative Damage: in vivo model(Wiley, 2017) Sahin, Nurhan; Akdemir, Fatih; Orhan, Cemal; Tuzcu, Mehmet; Yilmaz, Ismet; Juturu, Vijaya[Abstract Not Available]Öğe Apricot attenuates oxidative stress and modulates of Bax, Bcl-2, caspases, NF?-B, AP-1, CREB expression of rats bearing DMBA-induced liver damage and treated with a combination of radiotherapy(Pergamon-Elsevier Science Ltd, 2014) Karabulut, Aysun Bay; Karadag, Nese; Gurocak, Simay; Kiran, Tugba; Tuzcu, Mehmet; Sahin, KazimWe evaluated the ability of apricot to attenuate apoptosis and oxidative stress developed during the process of 7,12-dimethylbenz[a]anthracene (DMBA) and radiotherapy in the liver of rats bearing liver damage. Fifty female Wistar rats were divided into 7 groups; (i) normal control rats; (ii) rats fed with standard diet with apricot (20%), (ii) rats fed with standard diet and administrated 6 gray radiotherapy with Co 60 device applied to a single fraction, (iv) rats fed with standard diet and administered intraperitoneally DMBA (20 mg/kg), (v) rats fed with standard diet and administered DMBA and 6 gray radiotherapy, (vi) rats fed with standard rat diet and administered DMBA and supplemented apricot, (vii) rats fed with standard diet supplemented apricot administered DMBA and radiotherapy (RT) for 6 weeks. Expression of Bax, caspase 3, and glutathione activity decreased in the liver but liver expression of NF-kappa B, AP-1, CREB, Bcl-2 and ALT, AST, 5'NT, MDA, NO levels increased in DMBA-induced liver damage rats. In conclusion, the results suggest that apricot supplementation and irradiation given in combination, offer maximum protection against DMBA-induced hepatic carcinogenesis. (C) 2014 Elsevier Ltd. All rights reserved.Öğe Combinatorial effect of zoledronic acid and irradiation on the prevention of DMBA-induced precancerogenic changes in the mammary tissues of rats(Wolters Kluwer Medknow Publications, 2016) Gurocak, Simay; Karabulut, Aysun Bay; Tuzcu, Mehmet; Sahin, Nurhah; Temelli, Oztun; Ince, Volkan; Sahin, KazimBackground: At present, the rates of breast cancer are continuously increasing, with over a million new cases being diagnosed worldwide each year. Hence, the development of new breast cancer chemopreventive drugs with acceptable efficacy and toxicity that are suitable for use for a protracted period of time is urgently needed. The present study investigated the potential preventive effects of zoledronic acid [ZOL] and radiotherapy [RT], both alone and in combination, on precancerogenic changes on the breast tissues of females. Materials and Methods: Wistar rats were treated with 7,12-dimethylbenz [a] anthracene [DMBA] at the acute phase. Fifty female rats were divided into seven groups: Control group [I]; ZOL, group [II]; RT, group [III]; DMBA, group [IV]; DMBA + RT, group [V]; DMBA + ZOL, group [VI]; and DMBA + ZOL + RT, group [VII]. Results: The treatment of DMBA-exposed rats with ZOL and RT, both alone and in combination, successfully upregulates the transcriptional levels of Bax, caspase-3, caspase-9, p21, and BRCA 1 in mammary tissues, which may account for the elevated apoptotic activities observed and the eventual inhibition of tumor growth. The administration of RT and ZOL both alone and in combination was found to be effective for inhibiting the DMBA-induced precancerogenic changes on breast tissues and modulating the expression of apoptosis-associated proteins in the acute phase. Conclusions: The combination of RT and ZOL was more effective than either agent alone. Our results suggest that the administration of ZOL and irradiation in combination can offer maximal protection against DMBA-induced mammary precancerogenic changes.Öğe Comparative evaluation of the sexual functions and NF-?B and Nrf2 pathways of some aphrodisiac herbal extracts in male rats(Bmc, 2016) Sahin, Kazim; Orhan, Cemal; Akdemir, Fatih; Tuzcu, Mehmet; Gencoglu, Hasan; Sahin, Nurhan; Turk, GaffariBackground: Mucuna pruriens, Tribulus terrestris and Ashwagandha (Withania somnifera) are widely known as antioxidant effective herbals and have been reported to possess aphrodisiac activities in traditional usages. In this study, we determined the effects of these herbals on sexual functions, serum biochemical parameters, oxidative stress and levels of NF-kappa B, Nrf2, and HO-1 in reproductive tissues. Methods: Thirty-five male rats were divided into five groups: the control group, sildenafil-treated group (5 mg/kg/d), Mucuna, Tribulus and Ashwagandha groups. The extract groups were treated orally either with Mucuna, Tribulus or Ashwagandha (300 mg/kg b.w.) for 8 weeks. Results: All of the extracts were found to be significantly effective in sexual functioning and antioxidant capacity and Tribulus showed the highest effectiveness. Serum testosterone levels significantly increased in Tribulus and Ashwagandha groups in comparison to control group. Tribulus was able to reduce the levels of NF-kappa B and increase the levels of Nrf2 and HO-1 to a much greater extent than Mucuna and Ashwagandha. Conclusions: These results demonstrate for the first time that Mucuna, Tribulus and Ashwagandha supplementation improves sexual function in male rats via activating Nrf(2)/HO-1 pathway while inhibiting the NF-kappa B levels. Moreover, Tribulus terrestris extract was found to be more bioavailable from Ashwagandha extract followed by Mucuna extract.Öğe ?-Cryptoxanthin ameliorates metabolic risk factors by regulating NF-?B and Nrf2 pathways in insulin resistance induced by high-fat diet in rodents(Pergamon-Elsevier Science Ltd, 2017) Sahin, Kazim; Orhan, Cemal; Akdemir, Fatih; Tuzcu, Mehmet; Sahin, Nurhan; Yilmaz, Ismet; Juturu, VijayaThe aim of this experiment was to determine the effects of beta-cryptoxanthin (BCX) on the cardiometabolic health risk factors and NF-kappa B and Nrf2 pathway in insulin resistance induced by high-fat diet (HFD) in rodents. Twenty-eight Sprague-Dawley rats were allocated into four groups: (1) Control, rats fed a standard diet for 12 weeks; (2) BCX, rats fed a standard diet and supplemented with BCX (2.5 mg/kg BW) for 12 weeks; (3) HFD, rats fed a HFD for 12 weeks, (4) HFD + BCX, rats fed a HFD and supplemented with BCX for 12 weeks. BCX reduced cardio-metabolic health markers and decreased inflammatory markers (P < 0.001). Rats fed a HFD had the lower total antioxidant capacity and antioxidant enzymes activities and higher MDA concentration than control rats (P < 0.001 for all). Comparing with the HFD group, BCX in combination with HFD inhibited liver NF-kappa B and TNF-alpha expression by 22% and 14% and enhanced liver Nrf2, HO-1, PPAR-alpha, and p-IRS-1 by 1.43,1.41, 3.53, and 1.33 fold, respectively (P < 0.001). Furthermore, in adipose tissue, BCX up-regulated Nrf2, HO-1, PPAR-alpha, and p-IRS-1 expression, whereas, down-regulated NF-kappa B and TNF-alpha expression. In conclusion, BCX decreased visceral fat and cardiometabolic health risk factors through modulating expressions of nuclear transcription factors. (C) 2017 Elsevier Ltd. All rights reserved.Öğe Dietary arginine silicate inositol complex inhibits periodontal tissue loss in rats with ligature-induced periodontitis(Dove Medical Press Ltd, 2016) Dundar, Serkan; Eltas, Abubekir; Hakki, Ema S.; Malkoc, Siddik; Uslu, M. Ozay; Tuzcu, Mehmet; Komorowski, JamesThe purpose of this study was to induce experimental periodontitis in rats previously fed diets containing arginine silicate inositol (ASI) complex and examine the biochemical, immunological, and radiological effects. Fifty two 8-week-old female Sprague Dawley rats were equally divided into four groups. The control group included those fed a standard rat diet with no operation performed during the experiment. The periodontitis, ASI I, and ASI II groups were subjected to experimental periodontitis induction for 11 days after being fed a standard rat diet alone, a diet containing 1.81 g/kg ASI complex, or a diet containing 3.62 g/kg ASI complex, respectively, for 8 weeks. Throughout the 11-day duration of periodontitis induction, all rats were fed standard feed. The rats were euthanized on the eleventh day, and their tissue and blood samples were collected. In the periodontitis group, elevated tissue destruction parameters and reduced tissue formation parameters were found, as compared to the ASI groups. Levels of enzymes, cytokines, and mediators associated with periodontal tissue destruction were lower in rats fed a diet containing ASI complex after experimental periodontitis. These results indicate that ASI complex could be an alternative agent for host modulation.Öğe Dietary Mango Ginger May Enhance the Exercise Performance and Reduces Lipid Profile when Combined with Treadmill Running in a Rat Model(Wiley, 2017) Sahin, Kazim; Tuzcu, Mehmet; Orhan, Cemal; Sahin, Nurhan; Akdemir, Fatih; Pala, Ragip; Juturu, Vijaya[Abstract Not Available]Öğe Effect of inositol -stabilized arginine silicate on arthritis in a rat model(Pergamon-Elsevier Science Ltd, 2019) Sahin, Kazim; Ojalvo, Sara Perez; Akdemir, Fatih; Orhan, Cemal; Tuzcu, Mehmet; Sahin, Nurhan; Ozercan, Ibrahim H.The purpose of this study was to test the effects of arginine-silicate-inositol complex (ASI), compared to a combination of the individual ingredients (A + S + I) of the AM, on inflammatory markers and joint health in a collagen-induced arthritis (CIA) rat model. A total of 28 Wistar rats were divided into four groups: (i) Control; (ii) Arthritic group, rats subjected to CIA induction by injection of bovine collagen type II (A); (iii) Arthritic group treated with equivalent doses of the separate components of the ASI complex (arginine hydrochloride, silicon, and inositol) (A + S + I); (iv) Arthritic group treated with the ASI complex. The ASI complex treatment showed improved inflammation scores and markers over the arthritic control and the A + S + I group. ASI group had also greater levels of serum and joint-tissue arginine and silicon than the A + S + I group. Joint tissue IL-6, NF-kappa B, COX-2, TNF-alpha, p38 MAPK, WISP-1, and beta-Catenin levels were lower in the ASI group compared to the other groups (P < 0.05 for all). In conclusion, these results demonstrate that the ASI complex may be effective in reducing markers of inflammation associated with joint health and that the AM complex is more effective than a combination of the individual ingredients.Öğe The effect of lycopene on antioxidant status in rainbow trout (Oncorhynchus mykiss) reared under high stocking density(Elsevier Science Bv, 2014) Sahin, Kazim; Yazlak, Hasan; Orhan, Cemal; Tuzcu, Mehmet; Akdemir, Fatih; Sahin, NurhanLycopene, the major carotenoid found in tomato, exhibits health beneficial effects by virtue of its antioxidant activity. As stocking density is known as a stress factor in fish, the effects of dietary lycopene supplementation (0, 200 or 400 mg kg(-1)) on growth performance and antioxidant status in rainbow trout under normal (20 kg m(3-1)) and high (100 kg m(3-1)) stocking density were studied. Glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) activities were evaluated, as well as lipid peroxidation measured as malondialdehyde (MDA). Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappa B), erythroid-derived 2-like 2 (Nrf2) and heme-oxygenase 1 (HO-1) levels were also assayed as an indicator of detoxifying/antioxidant defense mechanisms to cope with oxidative stress. Results showed that exposure to high stocking density depressed feed intake by 6.8% and body weight gain by 12.1%, increased plasma and hepatic MDA level by 88.5 and 124.3% and decreased hepatic SOD, CAT and GSH-Px activities by 22.1, 43.4 and 51.7%, respectively. In fish under crowded conditions, an expression of hepatic NF-kappa B was increased by 71.5%, being suppressed in Nrf2 and HO-1 by 40.6 and 42.7%, respectively. Lycopene supplementation increased linearly fish growth performance and antioxidant enzyme activity, and decreased linearly MDA concentration in both low and high stocking densities. NF-kappa B levels decreased hepatic Nrf2 and HO-1 levels increased linearly with lycopene supplementation at a greater extent in fish under low stocking condition. In conclusion, dietary supplementation of lycopene to fish reduces the detrimental effects of high stocking density on growth performance and modulates oxidative status via activating host defense system at cellular level. It appears that lycopene can be added up to 400 mg kg(-1) to rainbow trout diets to improve meat quality. (C) 2013 Elsevier B.V. All rights reserved.Öğe The efficacy of dietary curcumin on growth performance, lipid peroxidation and hepatic transcription factors in rainbow trout Oncorhynchus Mykiss (Walbaum) reared under different stocking densities(Wiley, 2017) Akdemir, Fatih; Orhan, Cemal; Tuzcu, Mehmet; Sahin, Nurhan; Juturu, Vijaya; Sahin, KazimCurcumin, derived from Curcuma longa, exerts antioxidant, antibacterial, anticancer and immunomodulatory properties. This study was conducted to determine the effect of dietary water-soluble curcumin (0, 200 or 400mgkg(-1)) on growth performance and antioxidant status in rainbow trout (Oncorhynchus mykiss, Walbaum) rearing under normal (20kgm(-3)) or high (100kgm(-3)) stocking density conditions. A significant increase in body weight (FBW), feed intake (FI) and weight gain (BWG) was observed in fish fed with curcumin-supplemented diet at 200mgkg(-1) of diet levels of high stocking density conditions (P<0.0001), whereas feed conversion ratio (FCR) was not significant. Also, serum and liver malondialdehyde (MDA) levels were decreased by dietary curcumin in crowded fish (P<0.01, for both). With curcumin supplementation, expressions of heat shock protein 70 (HSP70; P<0.0001) and hepatic nuclear factor kappa-B (NF-B; P<0.01) decreased, whereas expressions of hepatic nuclear factor erythroid 2 (NF-E2)-related factor 2 (Nrf2) and heme oxygenase 1 (HO-1) (P<0.001, for both) increased in fish exposed to high stocking density. In conclusion, curcumin supplementation alleviates adverse effects of high stocking density on performance through modulating expressions of stress-related nuclear transcription factors in rainbow trout.Öğe Evaluation of antioxidant and antiproliferative activities of 1,2-bis (p-amino-phenoxy) ethane derivative Schiff bases and metal complexes(Wiley, 2019) Parlak, Akif Evren; Cakmak, Haluk; Sandal, Suleyman; Yilmaz, Bayram; Sekerci, Memet; Genc, Zuhal Karagoz; Tuzcu, MehmetIn this study, the effects of the two Schiff base derivatives and their metal complexes were tested for MDA concentration, which is an indicator of lipid peroxidation, antioxidant vitamin A, vitamin E, and vitamin C levels in cell culture. A comparison was performed among the groups and it was observed that MDA, vitamin A, vitamin E, and vitamin C concentrations were statistically changed. According to the results, all compounds caused a significant oxidative stress without Zn complexes. Moreover, Mn(II), Cu(II), Zn(II), and Ni(II) complexes of Schiff bases derived from a condensation of 1,2-bis (p-aminophenoxy) ethane with naphthaldehydes and 4-methoxy benzaldehyde were examined in terms of antitumor activity against MCF-7 human breast cancer and L1210 murine leukemia cells. Furthermore, the derivatives were tested for antioxidative and prooxidative effects on MCF-7 breast cancer cells. The compounds which were tested revealed that there was an antitumor activity for MCF-7 and L 1210 cancer cells. Also, some of the compounds induced oxidative harmful.Öğe High-fructose corn syrup causes vascular dysfunction associated with metabolic disturbance in rats: Protective effect of resveratrol(Pergamon-Elsevier Science Ltd, 2012) Akar, Fatma; Uludag, Orhan; Aydin, Ali; Aytekin, Yasin Atacan; Elbeg, Sehri; Tuzcu, Mehmet; Sahin, KazimHigh-fructose corn syrup (HFCS) is used in many prepared foods and soft drinks. However, limited data is available on the consequences of HFCS consumption on metabolic and cardiovascular functions. This study was, therefore, designed to assess whether HFCS drinking influences the endothelial and vascular function in association with metabolic disturbances in rats. Additionally, resveratrol was tested at challenge with HFCS. We investigated the effects of HFCS (10% and 20%) and resveratrol (50 mg/l) beverages on several metabolic parameters as well as endothelial relaxation, vascular contractions, expressions of endothelial nitric oxide synthase (eNOS), sirtuin 1 (SIRT1), gp91(phox) and p22(phox) proteins and superoxide generation in the aortas. Consumption of HFCS (20%) increased serum triglyceride, VLDL and insulin levels as well as blood pressure. Impaired relaxation to acetylcholine and intensified contractions to phenylephrine and angiotensin II were associated with decreased eNOS and SIRT1 whereas increased gp91(phox) and p22(phox) proteins, along with provoked superoxide production in the aortas from HFCS-treated rats. Resveratrol supplementation efficiently restored HFCS-induced deteriorations. Thus, intake of HFCS leads to vascular dysfunction by decreasing vasoprotective factors and provoking oxidative stress in association with metabolic disturbances. Resveratrol has a protective potential against the harmful consequences of HFCS consumption. (C) 2012 Elsevier Ltd. All rights reserved.Öğe Lutein and Zeaxanthin isomers (L/Zi) Modulate Photo-Oxidative Retinal Damage in an Animal Model(Assoc Research Vision Ophthalmology Inc, 2017) Juturu, Vijaya; Akdemir, Fatih; Orhan, Cemal; Tuzcu, Mehmet; Sahin, Nurhan; Yilmaz, Ismet; Sahin, Kazim[Abstract Not Available]Öğe Lutein and zeaxanthin isomers may attenuate photo-oxidative retinal damage via modulation of G protein-coupled receptors and growth factors in rats(Academic Press Inc Elsevier Science, 2019) Sahin, Kazim; Gencoglu, Hasan; Akdemir, Fatih; Orhan, Cemal; Tuzcu, Mehmet; Sahin, Nurhan; Yilmaz, IsmetBackground: Retina photoreceptor cells are specially adapted for functioning over comprehensive ambient light conditions. Lutein and Zeaxanthin isomers (L/Zi) can protect photoreceptor cells against excessive light degeneration. Efficacy of L/Zi has been assessed on some G protein-coupled receptors (GPCRs), transcription and neurotrophic factors in the retina of rats exposed to incremental intense light emitting diode (LED) illumination conditions. Methods: Forty-two male rats (age: 8 weeks) were randomly assigned to six treatment groups, 7 rats each. The rats with a 3x2 factorial design were kept under 3 intense light conditions (12hL/12hD, 16hL/8hD, 24hL/OhD) and received two levels of L/Zi (0 or 100 mg/kg BW) for two months. Increased nuclear factor-kappa B (NF-kappa B), glial fibrillary acid protein (GFAP), and decreased Rhodopsin (Rho), Rod arrestin (Sag), G Protein Subunit Alpha Transducin1 (Gnat1), neural cell adhesion molecule (NCAM), growth associated protein-43 (GAP43), nuclear factor (erythroid-derived 2)-like 2 (Nrf2), and heme oxygenase 1 (HO-1) were observed in 24 h light intensity adaptation followed by 16 h IL and 8 h D. Results: L/Zi administration significantly improved antioxidant capacity and retinal Rho, Rod-arrestin (Sag), Gnat1, NCAM, GAP43, BDNF, NGF, IG1, Nrf2, and HO-1 levels. However, the levels of NF-kappa B and GFAP levels were decreased by administration of L/Zi. Conclusions: According to these results, L/Zi may be assumed as an adjunct therapy to prevent early photoreceptor cell degeneration and neutralize free radicals derived from oxidative stress. (C) 2019 Elsevier Inc. All rights reserved.Öğe Lycopene improves activation of antioxidant system and Nrf2/HO-1 pathway of muscle in rainbow trout (Oncorhynchus mykiss) with different stocking densities(Elsevier, 2014) Sahin, Kazim; Orhan, Cemal; Yazlak, Hasan; Tuzcu, Mehmet; Sahin, NurhanThe purpose of this study was to describe the effect of lycopene on antioxidant status, heat shock protein 70 (Hsp70), nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and heme-oxygenase 1(HO-1) levels in the muscle of rainbow trout (Oncorhynchus mykiss). The fish were kept at low(20 kg m(3 - 1)) or high(100 kg m(3 - 1)) stocking densities till the end of study and fed a basal diet or the basal diet supplemented with either 200 or 400 mg of lycopene kg(-1) of diet. The protein, lipid and ash contents in the muscle were not changed by the supplementation of lycopene in rainbow trout kept under high or low stocking densities. In response to increasing supplemental lycopene level, there were linear increases in lycopene levels, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities (P < 0.001) and Nrf2 and HO-1 levels of muscle; linear decreases in muscle malondialdehyde (MDA) and Hsp70 levels (P < 0.001). In conclusion, lycopene supplementation to fish reduces the detrimental effects of oxidative stress and lipid peroxidation resulting from stocking densities via activating host defence system at cellular level. (C) 2014 Elsevier B.V. All rights reserved.Öğe MAT, a Novel Polyherbal Aphrodisiac Formulation, Enhances Sexual Function and Nrf2/HO-1 Pathway While Reducing Oxidative Damage in Male Rats(Hindawi Ltd, 2018) Sahin, Kazim; Tuzcu, Mehmet; Orhan, Cemal; Gencoglu, Hasan; Sahin, Nurhan; Akdemir, Fatih; Turk, GaffariMucuna pruriens, Ashwagandha, and Tribulus terrestris are known as the enhancers for sexual health, functional activities, vitality, and longevity. These herbs had been widely used in the Ayurveda medicine as aphrodisiacs through the ages, and their efficacy was also verified separately in our previous publication. Therefore, the aim of this study was to determine the effects of Mucuna, Ashwagandha, and Tribulus complexes on sexual function in rats. Twenty-eight male rats allocated to four groups as follows: (i) negative control (C); (ii) positive control or sildenafil citrate treated group (5 mg/kg) (S); (iii) MAT1 (combination of 10 mg Mucuna (M) + 10 mg Ashwagandha (A) + 10 mg Tribulus (T)/kg BW); (iv) MAT 2 (20 mg Mucuna + 20 mg Ashwagandha + 20 mg Tribulus/kg BW). There was no significant difference found between the MAT1 and MAT2 groups while they showed significantly increased testosterone, follicle-stimulating hormone (FSH), and luteinizing hormone (LH) levels when compared to the negative control. Significant increases in Nrf2/HO1 levels and decreases in NF-kappa B were detected in MAT groups similar to the decrease in serum and testis malondialdehyde (MDA) levels as compared to both controls. The spermmotility, count, and rate also significantly improved in both MAT groups, while ALT, AST, creatinine, ALP, and urea levels did not change in any of the groups. Oral consumption of MATs combination in male rats resulted in inhibition of NF-kappa B and MDA and also increased sex hormones with Nrf2-mediated HO-1 induction. MAT combinations may improve sexual functions by increasing levels of sexual hormones and regulation of NF-kappa B and Nrf2/HO-1 signaling pathways.Öğe Mesozeaxanthin Protects Retina from Oxidative Stress in a Rat Model(Mary Ann Liebert, Inc, 2016) Orhan, Cemal; Akdemir, Fatih; Tuzcu, Mehmet; Sahin, Nurhan; Yilmaz, Ismet; Deshpande, Jayant; Juturu, VijayaPurpose: Mesozeaxanthin (MZ) is able to protect against chronic and cumulative eye damage and neutralize free radicals produced by oxidative stress. The objective of the present study was to evaluate the protective potential of MZ against retinal oxidative damage and growth and transcription factors of the retina in rats fed with high-fat diet (HFD). Methods: Twenty-eight Sprague Dawley rats were randomly divided into the following 4 groups: (1) Control, (2) MZ (100 mg/kg bw/d), (3) HFD (42% of calories as fat), and (4) HFD+MZ (100 mg/kg bw/d) group rats were administered daily as supplement for 12 weeks. Results: Consumption of HFD was associated with hyperglycemia and oxidative stress as reflected by increased serum MDA concentration (P < 0.001). No measurable zeaxanthin (Z)+MZ and lutein (L) could be detected in the serum of control and HFD rats, whereas they were observed in the serum of MZ-administered rats. Retinal antioxidant enzyme [superoxide dismutase (SOD) and catalase (CAT)] activities were significantly decreased in the HFD group compared to the normal group (P < 0.01). However, retinal antioxidant enzymes were restored close to normal levels in HFD+MZ-treated rats (P < 0.05). The retina of rats fed with HFD had increased levels of vascular endothelial growth factor (VEGF), inducible nitric oxide (iNOS), intercellular adhesion molecule-1 (ICAM-1), and nuclear factor-kappa B (NF-kappa B) levels and decreased nuclear factor erythroid 2-related factor 2 (Nrf2) and heme-oxygenase 1(HO-1) levels compared to the healthy rat retina (P < 0.001). Rats treated with MZ partially alleviated the inflammation as reflected by suppressed VEGF, iNOS, ICAM, and NF-kB levels and increased Nrf2 and HO-1 levels in the retina of rats fed (P < 0.05). Conclusions: Results from the present study suggest that MZ has protective effects on the retina and the ability to modulate oxidative stress of retina in rats fed an HFD by suppressing retinal lipid peroxidation and regulating growth and transcription factors.Öğe Mesozeaxanthin protects the liver and reduces cardio-metabolic risk factors in an insulin resistant rodent model(Taylor & Francis Ltd, 2017) Sahin, Kazim; Orhan, Cemal; Akdemir, Fatih; Tuzcu, Mehmet; Sahin, Nurhan; Yilmaz, Ismet; Ali, ShakirBackground: Mesozeaxanthin (MZ) is a macular carotenoid which has been reported to have a number of pharmacological properties, including the antioxidant, and anticarcinogenic property, and has been stated to decrease the hepatocyte lipid content. Objective: In this study, we investigated the effect of MZ on cardio-metabolic health risk (CMHR) and its probable mechanisms of action in rats fed a high-fat diet (HFD). Design: Rats were randomly divided into four groups consisting of (i) Control, (ii) MZ, (iii) HFD, and (iv) HFD+MZ. Results: MZ treatment increased the antioxidant enzyme activities and helped improve the liver function. The treatment alleviated CMHR and decreased the level of nuclear factor kappa B (NF-kappa B p65) and tumor necrosis factor-alpha (TNF-alpha). The levels of hepatic peroxisome proliferator-activated receptor gamma (PPAR-gamma), phosphorylated insulin receptor substrate 1 (p-IRS-1), beta,beta-carotene 9', 10'-oxygenase 2 (BCO2) and nuclear factor erythroid 2-related factor 2 (Nrf2), which decrease in HFD rats, were found to be significantly higher in MZ supplemented animals. Conclusion: MZ has antioxidant and anti-inflammatory properties and can is reported in this study toprotect against fatty liver and cardio-metabolic syndrome, possibly through regulation of PPAR-gamma, IRS-1, Nrf2 and NF-kappa B proteins, in an insulin-resistant rodent model.Öğe A novel schiff base derivative for effective treatment of azoxymethane induced colon cancer(IJPSR, 2014) Doğan, Ayşegül; Başak, Neşe; Demirci, Selami; Telci, Dilek; Dede, Bülent; Tuzcu, Mehmet; Özercan, İbrahim Halil; Şahin, Kazım; Şahin, FikrettinThe field of cancer research has been emerged in recent years for the development of specific drugs to cancer treatment. New agents with the ability to provide efficient treatment by reducing side effects has led to new opportunities for improving agents for cytotoxic therapies. While there are several drugs for colon cancer treatment, researchers are trying to evaluate new agents or combinations of existing ones which can be used efficiently. Schiff bases with a wide range of variety and biological properties including anticancer activity might be used for colon cancer treatment. In the current study, a novel schiff base derivative synthesized by our group was tested in vivo for colon cancer. In a model of azoxymethane (AOM) induced colorectal cancer, chemopreventive properties of schiff base was also analyzed in rats. While AOM induced de novo crypt formation, adenocarcinoma and dysplasia development, schiff base application reduced the number of aberrant crypt foci (ACF), dysplasia or adenocarcinoma. Analysis of the intestinal mucosa showed that peritoneal administration of SB complex not only decreased the protein expression of COX-2, Bcl-2 and NF-κB but also enhanced the Bax expression suggesting the apoptotic and anti-proliferative effects for this compound. Our findings showed that SB complex might be used for the colorectal cancer treatment. Further studies are highly warranted to obtain additional insights and identify mode of action for the schiff base.