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  • Küçük Resim
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    Effects of ozone application on fissure sealants prepared different surface etching modalities: An in vitro study
    (2019) Surmelioglu, Derya; Ucar Gundogar, Zubeyde
    Aim: The aim of this study was to compare the microleakage levels of different surface treatment of fissure sealants. Material and Methods: A total of 60 standardized non-erupted third molar teeth were divided into 5 groups (n=12) according to the pre-treatment method; Group 1 (G1): Clearfil Universal Bond Quick (self-etching) + ozone +fissure sealant (FS), Group 2 (G2): Clearfil Universal Bond Quick (self-etching) +FS, Group 3(G3): Clearfil Universal Bond Quick (etch-and-rinse) +ozone +FS, Group 4 (G4): Clearfil Universal Bond Quick (etch-and-rinse) +FS, Group 5 (G5): phosphoric acid etching +FS (control). Following surface pre-treatments, fissures of all teeth were sealed. Then, all specimens were subjected to thermal cycles (5000 times of 5 degrees C/55 degrees C for 1 minute each). All surfaces of the teeth were covered with nail polish twice up to a distance of 1mm to fissure sealant. All teeth were immersed in a 0.5% basic fuchsine dye for 24 hours. To examine microleakage, teeth separated into 2 halves bucco-lingually with saw. Microleakage scores were evaluated under stereomicroscope with a magnification of x40. The scores were statistically analyzed with Kruskal-Wallis and Mann Whitney U tests. Results: G2, G3 and G4 showed statistically significant differences compared to control group, except G1. Although G2 showed highest microleakage level, G4 sowed lowest microleakage level G5 Ozone led to decreased microleakage in images in groups 3, 5 and 6. Conclusions: The fissure sealing ability of with the universal adhesive in etch-and-rinse or self-etch modes was similar to that of conventional acid etching. Ozone pretreatment favorably affected the marginal sealing ability of the tested fissure sealants.
  • Küçük Resim
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    Salivary cortisol and total antioxidant capacity levels of children with untreated dental caries
    (2020) Keskin, Gul; Ucar Gundogar, Zubeyde; Ozdogru, Hakan; Ulusal, Hasan; Gundogar, Hasan
    Aim: The study aimed to evaluate the relationship between the caries frequency, which was determined using different caries indexes and biological parameters such as salivary cortisol and total antioxidant capacity (TAC).Materials and Methods: 140 patients between the ages of 5-13 without systemic disease were included in the study. Saliva samples were collected from patients whose DMFT (dmft) and PUFA (pufa) indexes were recorded, and these samples were centrifuged at 5,000 rpm for 10 minutes. All samples were stored at -20 °C until measurement. Saliva cortisol and TAC levels were measured using the ELISA kit. All data were analyzed with SPSS v.22.Results: The mean DMFT/dmft and PUFA/pufa values were estimated as 6.72±4.37, 1.39±2.08, respectively. There was no significant correlation between caries indexes and biochemical parameters.However, there was a correlation between TAC and cortisol levels in saliva. Conclusion: Within the limitations of this study, it was determined that high risk of caries in patients could not affect salivary cortisol and TAC levels; however, there was a positive correlation between salivary cortisol and TAC levels. Further studies are needed to better understand the background of the pathophysiological changes in these biochemical parameters and their relationship with caries.