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    Antiinflammatory and antioxidant activities of different hemostatics and pulp capping materials applied on rat teeth Biochemical effects of direct pulp capping with different hemostatics
    (Bayrakol Medical Publisher, 2021) Saygin, Aysegul Goze; Unal, Murat; Ercan, Nazli; Demir, Pinar
    Aim: This study aimed to investigate the anti-inflammatory and antioxidant activities of different hemostatic agents and pulp capping materials. Material and Methods: Ninety-six Wistar albino mate rats were divided into the following groups: Sterile Saline (SS), Sodium Hypochlorite (NaOCl), Mecsina Hemostopper (MIAS). Occlusal cavities were prepared in the first molar teeth. Hemostatic agents were used to control bleeding and different pulp capping materials were applied on the exposed pulp area. Subgroups were created according to pulp capping materials (Dycal, Biodentine Theracal, MTA RepairHP). Half of the groups were left to wait for sacrifice on the 7th day and the others on the 28th day. Cardiac blood was taken to determine of antiinflammatory and antioxidant serum markers. Results: The use of different hemostatic agents in terms of antioxidant (CAT, GPX) and anti-inflammatory (IL1-beta, TNF-alpha, IL-6) activity was statistically significant (p <0.05). There was no statistical significance between groups for SOD (p> 0.05). IL-6 was significantly higher on the 7th day than on the 28th day (p <0.05). The and-inflammatory activity reduced from the 7th to the 28th day, in contrast, antioxidant activity induced. Discussion: The inflammation process was related to the hemostatic agents and the capping materials used in DPC treatment. MHS may be an alternative for DPC treatment for bleeding control.
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    Evaluation of the effectiveness of different hemostatics and bioactive materials on the success of vital pulp therapy
    (Bayrakol Medical Publisher, 2022) Saygin, Aysegul Goze; Unal, Murat; Candan, Merve; Demir, Pinar; Akinci, Levent; Goze, Omer Fahrettin
    Aim: The aim of this study was to evaluate the response of the pulp in combination of hemostatic agents with pulp capping materials (PCMs). Material and Methods: A total of 96 rats were used. Two molar teeth of each rat were included and all groups were created of 4 animals. In the occlusal cavities of the teeth, pulp perforations were performed for direct pulp capping. Thereafter, three different agents, which were forming the main groups [Group1: Sterile saline, Group2: Sodium hypochlorite, Group3 :Mecsina Hemostopper] at different periods of time used on pulp perforations and according to PCMs coated on the exposed pulp area, were divided into subgroups (Dycal, Biodentine, Theracal, and MTA Repair HP). Subsequently, all groups were left for two different waiting periods of 7 and 28 days. Half of the rats were sacrificed on the 7th and the remaining half were sacrificed on the 28th day, followed by micro-CT and histological analyzes. Results: When the results of the study were examined, a statistical difference was observed between groups in terms of dentine bridge (DB) formation on the 7th and 28th days, while there was no statistical difference between inflammatory cell response and DB quality. Micro-CT images showed no formation of DB on the 7th day, while DB formation was observed on the 28th day on specimens. Discussion: The combination of MHS and NaOCl may be preferable in order to provide pulp bleeding control in dental applications.
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    Pokeweed (Phytolacca americana L.) antiviral protein inhibits Zucchini yellow mosaic virus infection in a dose-dependent manner in squash plants
    (Tubitak Scientific & Technological Research Council Turkey, 2017) Sipahioglu, Hikmet Murat; Kaya, Ilhan; Usta, Mustafa; Unal, Murat; Ozcan, Dilek; Ozer, Meryem; Guller, Abdullah
    Pokeweed antiviral protein (PAP) of Phytolacca americana L. (pokeweed) is a single-chain ribosome-inactivating protein (RIP) characterized by its ability to depurinate plant ribosomes. Here, we isolated, cloned, and expressed the ribosome inactivating protein (RIP) gene, designated as pokeweed antiviral protein type 1 (PAP I), from the summer leaves of pokeweed collected from the Black Sea region (Turkey). Our findings presented here provide direct evidence that exogenous application of PAP I causes concentration-dependent inhibition of Zucchini yellow mosaic virus (ZYMV) infection on squash plants. Squash plants were exposed to PAP I protein with and without DMSO for four consecutive days. Regular spraying of approximately 30 kDa recombinant PAP I at 2 mu g mL(-1) concentration prevented treated plants from mechanical virus infection. PAP I showed antiviral activity in 9 plants out of 15 inoculated plants. Remarkably, simultaneous application of PAP, DMSO, and ZYMV did not prevent virus infection, suggesting that PAP did not have any effect on viral RNA. In the absence of ZYMV the purified peptide was not cytotoxic for squash plants, although a reduction of plant size, possibly caused by host ribosome depurination, was observed.