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    The effect of transmitted Er:YAG laser energy through a dental ceramic on different types of resin cements
    (Wiley, 2015) Tak, Onjen; Sari, Tugrul; Malkoc, Meral Arslan; Altintas, Subutayhan; Usumez, Aslihan; Gutknecht, Norbert
    Background and ObjectiveThe laser debonding procedure of adhesively luted all-ceramic restorations is based on the ablation of resin cement due to the transmitted laser energy through the ceramic. The purpose of this study was to determine the effect of Er:YAG laser irradiation transmitted through a dental ceramic on five different resin cements. Materials and MethodsFive different resin cements were evaluated in this study: G-Cem LinkAce, Multilink Automix, Variolink II, Panavia F, and Rely X Unicem U100. Disc shaped resin cement specimens (n=10) were fabricated for each group. A ceramic disc was placed between the resin cement discs and the tip of the handpiece of Er:YAG laser device. The resin cement discs were irradiated through the ceramic and the volume of the resin cement discs were measured using a micro-CT system before and after Er:YAG laser irradiation. The volume loss of the resin cement discs was calculated and analyzed with one-way ANOVA and Tukey-HSD tests. ResultsThe highest volume loss was determined in G-Cem (1.10.6mm(3)) and Multilink (1.3 +/- 0.1mm(3)) (P<0.05) groups, and the lowest volume loss was determined in Rely X (0.3 +/- 0.07mm(3)), Variolink (0.4 +/- 0.2mm(3)), and Panavia (0.6 +/- 0.2mm(3)) groups (P<0.05). All resin cements were affected by the laser irradiation resulting in the volume loss of the cement; however, there are significant differences among different resin cements. ConclusionsAll the resin cements tested in this study were effected by the Er:YAG laser irradiation and there were significant differences among the resin cements with regard to ablation volume. Lasers Surg. Med. 47:602-607, 2015. (c) 2015 Wiley Periodicals, Inc.
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    Effects of laser irradiation at different wavelengths (660, 810, 980, and 1064 nm) on transient receptor potential melastatin channels in an animal model of wound healing
    (Springer London Ltd, 2015) Isman, Eren; Aras, Mutan Hamdi; Cengiz, Beyhan; Bayraktar, Recep; Yolcu, Umit; Topcuoglu, Tolga; Usumez, Aslihan
    The aim of the present study was to compare the effectiveness of four different laser wavelengths used for low-level laser therapy(LLLT) on healing of mucositis in an animal model of wound healing, by investigating expression of transient receptor potential melastatin(TRPM) ion channels. Forty-five rats were intraperitoneally injected with 100 mg/kg 5-fluorouracil on day 1 and 65 mg/kg on day 3. Superficial scratching on left cheek pouch mucosa was performed on days 3 and 5. After ulcerative mucositis was clinically detected, LLLT was started (660 nm, HELBO; 810 nm, Fotona-XD; 980 nm, ARC-Fox; and 1064 nm, Fidelis-Plus3) at 8 J/cm(2)/day from days 1 to 4. Oval excisional biopsy was performed at the wound site, and expression of TRPM2 to TRPM8 was evaluated. Student's t test was used for evaluation of significance of TRPM gene expression according to 0 value (alpha = 0.05). In 980-nm group, TRPM4, TRPM6, and TRPM7 were significantly higher than in the control group (p < 0.005). In 660, 810, and 1064 nm groups, only TRPM6 was significantly higher than in control group (p < 0.005). There were no significant differences between control and sham groups (p > 0.05). These findings suggest that expression of TRPM6 gene was significantly affected by irradiation with lasers at different wavelengths, whereas the TRPM4 and TRPM7 genes were only expressed in the 980-nm diode laser group. TRPM6 gene was highly expressed during LLLT, which may lead to accelerated wound healing and tissue repair. In contrast, there was some evidence that the 980-nm diode laser caused increased expression of TRPM4, TRPM6, and TRPM7 which are responsible for stimulation of Ca2+ and Mg2+ metabolism, as well as apoptotic pathways of controlled cell death.
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    Healing of surgical wounds treated with 810nm, 940nm, and 980nm diode lasers in different operation modes
    (2019) Aytugar, Emre; Unver, Tugba; Bicer Aytugar, Tugce; Celikten, Mert; Selvi, Firat; Alatli, Fatma Canan; Usumez, Aslihan
    Aim: The aim of the present study was to analyze the comparative effectiveness of the diode lasers having 3 different wave-lengths (810nm, 940nm, 980nm) and also the different application modes on the cellular basis in animal models.Many surgical instruments like scalpel, electrosurgery, and different laser types can be used for surgical incisions. Each of these have their own advantages and disadvantages.Material and Methods: 10 mm in length and 1 mm in depth dorsal skin incisions were created on 18 male Wistar albino rats via 3 different laser wave-lengths: 810nm (Gigaa, Cheese, China), 940nm(Epic 10, Biolase, USA) and 980nm (SIROlaser Advance, Sirona, Bensheim, Germany). The animals were sacrificed at either post-operative 24 hours, 3 days or 1 week. All incision sites were removed by excisional biopsies and the samples underwent histopathological evaluation.Results: There were no statistically significant differences in thermal changes, inflammation, and fibrosis in all wave-length groups (p>0.05). The chopped mode of irradiation of all wave-lengths showed significant increase in epithelial closure following the operation (p0.05). There were significant increases of the granulation tissue formation on the 3rd post-operative day with 940nm using chopped mode and 810nm in continuous mode (p0.05).Conclusion: Pulsed mode of lasers accelerates the epithelization regardless of the wave-lengths while 940nm and 810nm lasers in continuous mode speeds up the granulation tissue formation.Keywords: Diode lasers; wound healing; surgery.