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Öğe Determination of some trace and heavy elements, in vitro antioxidant activity, total phenolic and Phenethyl isothiocyanate content in Watercress from Turkey(United Arab Emirates Univ, 2022) Uyumlu, Ayse Burcin; Yilmaz, Hatice CaglarThe purpose of this study was to define the content of total phenolic and phenylethyl isothiocyanate, the in vitro antioxidant activity, and the content of Trace and Heavy Elements of watercress (Nasturtium officinale R. Br., Brassicaceae). It has ecological and economic importance and has been used by humans for many years both for food and medical purposes. Now it is reported to have a chemopreventive effect. In this study, Watercress samples were collected from Malatya, Turkey. Spectrophotometric methods were used to determine the antioxidant activity and the content of total phenolic and phenylethyl isothiocyanate. To determine heavy metals and trace elements watercress were used inductively coupled plasma-mass spectrometry (ICP-MS). Our results indicated that the extract of watercress has a high antioxidant capacity. This feature was related to phenolic compounds and phenylethyl isothiocyanate. Our analysis with ICP-MS has revealed that the watercress plant has high iron content and toxic heavy metals did not bioaccumulate in the watercress plant. So it is recommended to consume the watercress plant as food according to these results. However, toxicity studies should be carried out in living beings regarding plant intake.Öğe Development of Some Antiplatelet Salts as Drug Active Ingredients and Investigation Biological Activities(Springer, 2023) Uslu, Harun; Satilmis, Basri; Uyumlu, Ayse Burcin; Saglik, Begum Nurpelin; Levent, Serkan; Ozkay, Yusuf; Genc, Metin FikretIn this study, prasugrel and prasugrel salts: prasugrel + trans-1,2-cyclohexanedicarboxylic acid (1a), prasugrel + maleic acid (2a), prasugrel + citric acid (3a), prasugrel + oxalic acid (4a), and prasugrel + trometamol (5a) were synthesized and investigated for their biological activities.Ex vivo antiplatelet and in vitro cytotoxic activities were experimentally researched. Antiproliferative activity was not observed for prasugrel and prasugrel salts, and it was also determined that there was no cytotoxic effect. Ex vivo experiments were carried out on diabetic and nondiabetic rats. When the study results were examined, 2a and 3a were seen to be effective prasugrel salts for antiplatelet activity.Öğe Evaluation of Oxidant Injury Induced by Irradiation in Brain Tissues of Rats of Different Ages(Aves Yayincilik, Ibrahim Kara, 2009) Uyumlu, Ayse Burcin; Erkal, Haldun Suekrue; Batcioglu, Kadir; Serin, Meltem; Yucel, NeslihanObjectives: We aimed to evaluate the age-related changes of oxidative injury in the brain tissues of rats produced by radiotherapy that is widely used on cancer treatment. Study Design: Fifty-five male Wistar albino rats [ages of rats were 1, 4, 12 weeks (n=10) and 1 year (n=5)] were divided into four groups. Irradiation were performed on a Cobalt-60 unit using a single fraction of 8 Gy. The brain tissues were homogenized and divided into two portions. One portion was used for the measurement of the malondialdehyde (MDA). The other portion was used for the measurement of the protein concentration, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHPx) enzyme activities. Results: The SOD activity decreased significantly in 1-week-old rats (p<0.05). The decreases in GSHPx and CAT activities were more obvious in 1-week and 1-year-old rats than that in others. No significant changes were observed in adolescent and adult rats. The MDA levels of all groups increased. The highest MDA levels were seen in 1-year-old rats (p<0.05). Conclusion: Ionizing radiation used in radiotherapy affected antioxidant systems and increased MDA levels. These changes were more in the 1-week and 1-year-old rats than in others. This can be due to incomplete development of many systems in newborn rats and the loss of physiological capacities associated with aging in 1 -year-old rats.Öğe Hepatic Arginase - Nitric oxide imbalance: Impact of carcinogenesis and therapeutic effect of sodium channel blockage in an in vivo rat model(Walter De Gruyter Gmbh, 2016) Batcioglu, Kadir; Yildirim, Battal; Satilmis, Basri; Uyumlu, Ayse Burcin; Genc, Metin Fikret; Bentli, Recep; Djamgoz, Mustafa B. A.Objective: Nitric oxide synthase and arginase are frequently antagonistic and interactive, although both use L-arginine as common substrate. Their balance is of potential functional importance. How the balance changes in cancer is unknown. Increasing evidence suggests that progression of carcinomas involves functional voltage-gated sodium channel (VGSC) activity. Methods: The present study extended this study to liver and aimed to determine whether (i) DMBA carcinogenesis would affect the activities of arginase and NOS and (ii) treatment with Na-channel blocker RS100642 would ameliorate the impact of the carcinogen on the arginase-NOS balance. Results: DMBA application significantly increased arginase activity and, correspondingly, the level of L-ornithine by 25-33%. In contrast, NOS activity decreased by 11%. Importantly, RS100642 treatment completely suppressed the effect on arginase. Conclusion: It is concluded (i) that DMBA carcinogenesis changes the hepatic arginase-NOS balance, increasing the overall dominance of arginase and (ii) that VGSC inhibition has a protective effect on liver.Öğe Neuroprotective effects of the new Na channel blocker rs100642 in global ischemic brain injury(Termedia Publishing House Ltd, 2019) Kamisli, Suat; Basaran, Cenk; Batcioglu, Kadir; Oztanir, Mustafa Namik; Gul, Mehmet; Satilmis, Basri; Uyumlu, Ayse BurcinIntroduction: RS100642, a mexiletine analogue, is a novel sodium channel blocker with neuroprotective and antioxidant activities. The protectivity of RS100642, which has been shown against focal cerebral ischemia, was investigated in global cerebral ischemia in this study. Material and methods: Global cerebral ischemia was induced for five minutes in adult male Wistar Albino rats via the 4-vessel occlusion method. Intravenous administration of 1 mg/kg RS100642 following reperfusion for 30 min (RS100642 group) was compared with a sham treatment group (ischemia group) and nonischemized group (control) histologically based on morphology and caspase-3 immunohistochemistry, and biochemically based both on measurement of oxidative stress including malondialdehyde (MDA) levels, superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) activities and on assessment of apoptosis including caspase-3 and -8 activities and tumor necrosis factor alpha (TNF-alpha) levels at the end of 6 h. Results: While the RS100642 group had significantly lower MDA levels and higher SOD activities than the sham treatment group (p < 0.05), GPx and CAT activities of the RS100642 and sham treatment groups were similar (p > 0.05) and significantly lower than those of the controls (p < 0.05). Necrosis and caspase-3 activity and immunoreactivity in the RS100642 group were significantly lower than those in the sham treatment group (p < 0.05), while there was no significant difference between groups regarding caspase-8 and TNF-alpha (p > 0.05). Conclusions: Na+ channel blockade by RS100642 has remarkable neuroprotective effects following global brain ischemia/reperfusion damage. Further research is required to determine the optimum dose and time of administration.Öğe Phenethyl isothiocyanate protects against cyclophosphamide-induced nephrotoxicity via nuclear factor E2-related factor 2 pathway in rats(Sage Publications Ltd, 2023) Uyumlu, Ayse Burcin; Satilmis, Basri; Atici, Bugrahan; Taslidere, AsliPhenethyl isothiocyanate (PEITC), a secondary metabolite in Cruciferous plants, exerts chemopreventive and antioxidant effects. However, its therapeutic potential in cyclophosphamide (CP)-induced nephrotoxicity is not clear. So, we focused to research on the effect of PEITC against renal toxicity caused by CP and its relationship to the Nrf2 signaling mechanism. Thirty female Wistar albino rats were allocated to three groups: control (n = 10), CP (n = 10), and PEITC-pretreated group (150 mu mol/kg b.w. orally; n = 10). The antioxidant enzyme activities and levels of malondialdehyde (MDA), sirtuin 1 (SIRT1), glutathione-S-transferase (GST), nuclear factor E2-related factor 2 (Nrf2), nuclear factor kappa B (NF-kappa B), serum urea, and creatinine (Cr) were measured. In the CP group, serum urea and Cr, MDA, and NF-kappa B levels have risen, and the activities of antioxidant enzymes and SIRT1, Nrf2, and GST levels have reduced significantly (P < 0.05). PEITC diminished levels of Cr, urea, MDA, and NF-kappa B while it enhanced antioxidant enzyme activities and GST, Nrf2, and SIRT1 levels significantly (P < 0.05). Pretreatment with PEITC ameliorated kidney tissue injury. The renal protective effect of the PEITC was supported by the histological analysis of the kidney. PEITC prevented CP-induced nephrotoxicity by decreasing oxidative damage through Nrf2 and SIRT1 activation and NF-kappa B inhibition. Therefore, we have suggested that PEITC may be a useful agent for protection against CP-induced renal injury.Öğe POLLEN AND FUNGAL SPORE COMPOSITION VARIATIONS OF HONEYS ACCORDING TO DIFFERENT FEEDING METHODS(Pakistan Botanical Soc, 2023) Yil, Gulsah; Karlidag, Semiramis; Akyol, Abuzer; Koseman, Abdurrahman; Uyumlu, Ayse Burcin; Yilmaztekin, Murat; Erdogan, SelimHoney samples were collected from two districts of Malatya (Eastern Turkey) Battalgazi and Dogansehir for melissopalynological analysis after the honey season in 2018. The survey was conducted to 3 different feeding groups, Glucose, Sucrose, Bee Feed and a Control group in each study area. The samples were prepared according to Louveaux et al., (1978) procedure. Melissopalynological examinations were done to determine both pollen grains and fungal spores for each feeding group and districts. According to the microscopic analyses the dominant pollen types were determined as Astragalus, Cistus, Poaceae, Verbascum, Echium, Berberis, Artemisia, Plantago, Vicia, Onobrychis, Cichorioideae, Astragalus pollen grains were most frequent in glucose and control groups, Cistus pollen grains were widely represented in sucrose and bee feed groups in both study area. Dominant fungal spore types were determined as Aspergillus / Penicillium, Urediniospores, Cladosporium and Myrotechium. Aspergillus / Penicillium spores were dominated almost all samples by varying degrees. Analysis of pollen grains and fungal spores is useful instrument for determining the botanical, geographical and ecological sources of honey. This paper could be guide to beekeepers for selecting convenient apiary domains and appropriate feeding methods for qualitative honeys and the study is also help agriculturists for increasing the crop yield, the bees could be diverting to less-preferred plants during forages by sugar manipulation in order to cultivation improving.Öğe Protective effect of Lycium barbarum on renal injury induced by acute pancreatitis in rats(Wolters Kluwer Medknow Publications, 2022) Dogan, Tugba; Batcioglu, Kadir; Kustepe, Elif Kayhan; Uyumlu, Ayse Burcin; Yilmaztekin, YakupBackground: Lycium barbarum (LB) is a plant species that is well known in Chinese traditional medicine and is also considered a nutrient, belonging to the Solanaceae family, also called goji berry or wolfberry. Objectives: The aim of this study was to investigate the protective efficacy of LB, in kidney damage caused by acute pancreatitis (AP). Materials and Methods: In the study, we used 36 female Wistar albino rats (12 in each group) which were divided into three groups: Control, cerulein (100 mu g/kg b. wt. intraperitonally) and Cerulein + LB (6 mg/ml/day gastric gavage) group. Serum lipase, Interleukin (IL)-1, and IL-6 levels were measured. Superoxide dismutase, catalase (CAT), glutathione peroxidase enzyme activity assays and 8-hydroxy deoxyguanosine, malondialdehyde (MDA), and protein levels were measured in kidney tissue samples. In addition, histopathological analysis was performed in kidney tissue samples. Results: According to the findings, in the AP model created with Cerulein, administration of LB plant extract decreased oxidative stress and damage caused by AP in the kidney tissue and partially suppressed the inflammatory reactions in the tissue. Conclusion: According to the findings, in the AP model created with Cerulein, administration of LB extract decreased oxidative stress and in kidney damage caused by AP.Öğe Synthesis of PEITC-loaded gold nanoparticles and evaluation of the hepatoprotective effect on CCl4-induced damage through Nrf2 pathway(Walter De Gruyter Gmbh, 2023) Uyumlu, Ayse Burcin; Yilmaz, Hatice Caglar; Satilmis, Basri; Erdemoglu, SemaObjectives: The purpose of the study was to prepare an effective and new drug delivery system for enhancing the stability of Phenethyl isothiocyanate (PEITC), and its hepatoprotective effect in the carbon tetrachloride (CCl4)-induced damage in hepatocellular carcinoma G2 (HepG2) cell line via nuclear factor (erythroid-derived 2)-like 2 (Nrf2) pathway.Methods: Gold nanoparticles were synthesized and then characterized by XRD, SEM, SEM-EDX analysis, hydrodynamic diameter and zeta potential measurements. 1.0024 mM PEITC, a naturally occurring isothiocyanate, an active ingredient was loaded onto the characterized AuNPs. The cytotoxicity test of PEITC-AuNP and effects on ALT, AST, Nrf2 levels and total antioxidant capacity (TAC) of CCI4-induced HepG2 cells were investigated.Results: PEITC-AuNPs and PEITC decreased ALT and AST levels (p < 0.05). This reduction was greater with PEITC-AuNPs. PEITC-AuNPs increased Nrf2 level but it was nonsignificantly (p > 0.05). PEITC didn't increase the Nrf2 level in CCI4-induced HepG2 cells. TAC of both PEITC-AuNPs and PEITC administration increased significantly compared with CCl4 group (p < 0.05). But PEITC-AuNPs enhanced the TAC level higher than PEITC significantly (p < 0.05).Conclusions: PEITC-AuNPs were more effective than PEITC which resulted in more hepatoprotective and antioxidant effects via Nrf2 activation against CCl4-induced liver injury in HepG2 cells.Öğe Testosterone and DHEA-S levels with chronic tic disorder in children(Elsevier Science Bv, 2016) Erbay, Lale Gonenir; Kartalci, Sukru; Ozcan, Ozlem Ozel; Demir, Arzu Caliskan; Uyumlu, Ayse Burcin; Kutuk, Meryem OzlemThe neurobiological basis of tic disorders is thought to be a series of interactions including genetic, environmental and gender related factors. Being male is thought to be an especially important risk factor in the pathogenesis of tics. Our aim in this study was to investigate gender-related hormones such as testosterone, dehydroepiandrosterone sulfate (DHEA-S) and cortisol in tic patients. A total of 26 patients who had not entered puberty and had been diagnosed with chronic tic disorder and 25 healthy children were included in the study, Serum total testosterone, cortisol and DHEA-S levels were measured and the relationship with clinical data was investigated. The testosterone and DHEA-S levels of the patient group were higher than that of the control group (P=0.019, P=0.025) but no statistical difference was found between the cortisol levels (P=0.642). No statistical correlation was found between total tic severity, general disturbance, movement tic subscale scores and the DHEA-S (P=0.77, P=0.45, P=0.819 respectively) and testosterone levels (P=0.954, P=0.669, P=0.909 respectively). The results of this study reveal an elevation of testosterone and DHEA-S levels in patients. Future studies with a larger number of patients are likely to help elucidate the importance of these androgens in tic disorder. (C) 2016 Elsevier Inc. All rights reserved.
