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    Effects of irisin and exercise on adropin and betatrophin in a new metabolic syndrome model
    (Taylor & Francis Ltd, 2024) Aydin, Suna; Kilinc, Faruk; Ugur, Kader; Aydin, Mustafa Ata; Yalcin, Mehmet Hanifi; Kuloglu, Tuncay; Tektemur, Nalan Kaya
    Metabolic syndrome (MetS) is a prevalent public health problem. Uric acid (UA) is increased by MetS. We investigated whether administration of UA and 10% fructose (F) would accelerate MetS formation and we also determined the effects of irisin and exercise. We used seven groups of rats. Group 1 (control); group 2 (sham); group 3 (10% F); group 4 (1% UA); group 5 (2% UA); group 6 (10% F + 1% UA); and Group 7, (10% F + 2% UA). After induction of MetS (groups 3 -7), Group 3 was divided into three subgroups: 3A, no further treatment; 3B, irisin treatment; 3C, irisin treatment + exercise. Group 4, 1% UA, which was divided into three subgroups: 4A, no further treatment; 4B, irisin treatment; 4C, Irisin treatment + exercise. Group 5, 2% UA, which was divided into three subgroups: 5A, no further treatment; 5B, irisin treatment; 5C, irisin treatment + exercise. Group 6, 10% F + 1% UA, which was divided into three subgroups: 6A, no further treatment; 6B, irisin treatment; 6C, irisin treatment + exercise. Group 7, 10% F + 2% UA, which was divided into three subgroups: 7A, no further treatment; 7B, irisin treatment; 7C, irisin treatment + exercise., Irisin was administered 10 ng/kg irisin intraperitoneally on Monday, Wednesday, Friday, Sunday each week for 1 month. The exercise animals (in addition to irisin treatment) also were run on a treadmill for 45 min on Monday, Wednesday, Friday, Sunday each week for 1 month. The rats were sacrificed and samples of liver, heart, kidney, pancreas, skeletal muscles and blood were obtained. The amounts of adropin (ADR) and betatrophin in the tissue supernatant and blood were measured using an ELISA method. Immunohistochemistry was used to detect ADR and betatrophin expression in situ in tissue samples. The duration of these experiments varied from 3 and 10 weeks. The order of development of MetS was: group 7, 3 weeks; group 6, 4 weeks; group 5, 6 weeks; group 4, 7 weeks; group 3, 10 weeks. Kidney, liver, heart, pancreas and skeletal muscle tissues are sources of adropin and betatrophin. In these tissues and in the circulation, adropin was decreased significantly, while betatrophin was increased significantly due to MetS; irisin + exercise reversed this situation. We found that the best method for creating a MetS model was F + UA2 supplementation. Our method is rapid and simple. Irisin + exercise was best for preventing MetS.
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    Effects of Vitamin D on adropine and apoptosis in kidney tissue
    (2019) Onalan, Erhan; Demircan, Selcuk; Aydin, Suleyman; Kuloglu, Tuncay; Yalcin, Mehmet Hanifi; Gozel, Nevzat; Donder, Emir
    Aim: This study aims to investigate the effects of vitamin D on adropin and apoptosis in rat kidney tissue in the context of the experimental diabetes model created using streptozotocin (STZ).Material and Methods: 41 male Wistar-albino breed rats of 8-10 weeks were distributed into 5 groups, which consisted of 3 groups with 7 animals each and 2 groups with 10 animals each. No treatments were applied to the control group. The Buffer group was administered with single-dose 0.1 M sodium buffer intraperitoneally (ip). The Vitamin D group was orally administered 200 IU/day vitamin D. The Diabetes group was injected ip with single-dose 50 mg/kg STZ by dissolving the material in 0.1 M sodium buffer.Results: The biochemical and histological investigations revealed similar serum TOS and TAS levels, and TUNEL positivity and Adropin immunoreactivity for the Control, Buffer, and Vitamin D groups. While TOS levels and TUNEL positivity were significantly higher in the Diabetes group compared to the Control group, TAS levels and Adropin immunoreactivity were significantly lower. The TOS levels and TUNEL positivity were significantly reduced in the Diabetes+Vitamin D group compared to the diabetic group, and TAS levels, adropin immunoreactivity were significantly higher. Conclusion: In conclusion; it was determined that experimental diabetes increased TOS and apoptotic cells and decreased TAS and adropin levels in the kidney tissue in experimental diabetes, and that Vitamin D administered as treatment decreased TOS and apoptotic cells and increased TAS and Adropin levels. It was concluded that in order to uncover the role of diabetes in the pathophysiology of its effect on kidney tissue, future studies that consider various experimental diabetes times were necessary.