Yazar "Yeşilada Ö." seçeneğine göre listele

Listeleniyor 1 - 3 / 3
  • Küçük Resim Yok
    Öğe
    Laccase Production of Newly Isolated Trametes versicolor under Batch, Repeated-Batch, and Solid-State Fermentation Processes
    (ABADER (Adıyaman Bilimsel Arastırmalar Dernegi), 2022) Tutal T.; Yeşilada Ö.; Boran F.
    In this study, the laccase production ability of the newly isolated Trametes versicolor strain was investigated in three different fermentation processes. In all three fermentation processes, the fungus was able to produce the laccase enzyme. During the solid-state fermentation process 13.21 U/mL laccase activity was detected on the 20th day in the 10 mM copper-containing medium, while this value reached to 27.30 U/mL in the medium containing 0.5 mM ABTS+10 mM copper. During the liquid batch fermentation process, laccase activity was significantly induced in the medium containing 1 mM copper and the laccase activities reached 2.25, 19.83 and 24.57 U/mL compared to the medium without copper on the 3rd, 6th, and 9th days, respectively. ABTS and xylidine induced the laccase production of this strain at a much lower level than copper. The liquid repeated-batch process also significantly induced the laccase production. While low level of enzyme activities were detected in a copper-free medium, laccase activities were induced in the copper-containing medium and the activity increased from 0.66 U/mL to 9.87 U/mL at the 6th use of the pellets. Copper was detected as an effective inducer for laccase production in all fermentation processes and activity staining after native polyacrylamide gel electrophoresis clearly showed the active laccase bands. The results revealed that this strain is a good laccase producer and the laccase production yield varies depending on the fermentation process, production time, and inducer used. © 2022, ABADER (Adıyaman Bilimsel Arastırmalar Dernegi). All rights reserved.
  • Küçük Resim Yok
    Öğe
    Optimization of aniline biopolymerization reaction conditions by response surface methodology (RSM)
    (Science and Engineering Research Support Society, 2014) Aldemir A.; Birhanlı E.; Aktaş N.; Yeşilada Ö.
    Response Surface Methodology(RSM) was applied to enzymatic polymerization of aniline, which was conducted in a closed system containing sodium acetate buffer and presence of poly(sodium 4-styrene sulfonate) (SPS) polyelectrolyte as a template with laccase enzyme to produce water soluble polyaniline. Laccase enzyme used as catalyst was isolated from Trametes versicolor (ATCC 200801). The enzymatic polymerization rate of aniline based on measurements of initial dissolved oxygen (DO) consumption rate in the closed system, was optimized by the application of RSM. The independent variables, which had been found the most effective variables on the initial DO consumption rate by screening experiments, were determined as monomer concentration, medium temperature and medium pH. A quadratic model was developed through RSM in terms of related independent variables to describe the initial DO consumption rate as the response. Based on statistic analysis, optimum reaction conditions for the maximum initial DO consumption rate determined 42.50 mM, 39.34 °C and 5.45 as monomer concentration, medium temperature and medium pH, respectively. © 2014 SERSC.
  • Küçük Resim Yok
    Öğe
    Toxic effects of biodegraded and detoxified olive oil mill wastewater on the growth of Pseudomonas aeruginosa
    (Gordon & Breach Science Publ Inc, Newark, 1998) Yeşilada Ö.; Şam M.
    Detoxification and biodegradation of olive oil mill wastewater (OOMW) and toxicity (antibacterial effect) of untreated and treated (detoxified with Trametes versicolor) OOMW on a soil bacterium, P. aeruginosa were determined. T. versicolor biodegraded and detoxified OOMW. This research showed that T. versicolor can be satisfactorily used for biodegradation and detoxification of this waste.Detoxification and biodegradation of olive oil mill wastewater (OOMW) and toxicity (antibacterial effect) of untreated and treated (detoxified with Trametes versicolor) OOMW on a soil bacterium, P. aeruginosa were determined. T. versicolor biodegraded and detoxified OOMW. This research showed that T. versicolor can be satisfactorily used for biodegradation and detoxification of this waste.