Yazar "Yesilada E." seçeneğine göre listele

Listeleniyor 1 - 4 / 4
  • Küçük Resim Yok
    Öğe
    Genotoxicity testing of tributyltin and methidathion in drosophila melanogaster using the wing somatic mutation and recombination test
    (Parlar Scientific Publications, 2014) Karabulut A.K.; Yesilada E.
    In this study, genotoxic effects of tributyltin (TBT) and methidathion (MD) were investigated using Drosophila wing somatic mutation and recombination test (SMART). This test is based on the principle that the loss of heterozygosity of suitable recessive marker hairs such as multiple wing (mwh) and flare-3 (flr3), can lead to the formation of mutant clones of larval cells, which are then expressed as spots on the wings of the adult flies. Third-instar larvae that were trans-heterozygous for the two genetic markers mwh and flr3 were treated at different doses (0,1 mg/L, 1 mg/L, 10 mg/L, 30 mg/L, and 50 mg/L of TBT and 0.01 mg/L, 0.025 mg/L, 0.05 mg/L, 0.06 mg/L, and 0.075 mg/L of MD) of the test compounds. Mitomycin C (MMC), a commonly known mutagen, was used as positive control. The results indicated that highest experimental dose of TBT showed toxic and genotoxic effects, including recombinogenic activity in the marker-heterozygous flies. However, based on our results, MD showed no genotoxicity in the SMART assay, an in vivo model.
  • Küçük Resim Yok
    Öğe
    The investigation of polymorphisms in DNA repair genes (XRCC1, APE1 and XPD) in women with polycystic ovary syndrome
    (Asian Pacific Organization for Cancer Prevention, 2017) Gulbay G.; Yesilada E.; Celik O.; Yologlu S.
    Background: PCOS was reported to arise from the interaction of genetic and environmental factors. Some studies reported that women with PCOS have DNA damage and chromosome breakage. Such studies bring to mind the genes that are involved in DNA repairing. At present, several DNA repair genes and, as products of these genes, certain polymorphisms that alter the activity of proteins are known in the literature. The aim of this dissertation is to study the genomic instability that have been reported in PCOS cases along with the relationship between XRCC1 Arg194Trp, XRCC1 Arg399Gln, APE1 Asp148Glu, and XPD Lys751Gln polymorphisms in order to contribute to the pathogenesis of PCOS. Methods: Polymorphisms in DNA repair genes have been associated with the increased risk of various diseases and could also be related to the etiology of PCOS. Therefore, we conducted a study including 114 women with PCOS and 91 controls. These polymorphisms were determined by quantitative real time PCR and melting curve analysis using LightCycler. Results: Comparing the control groups at the end of the study, the results have not shown any statistically significant difference as far as XRCC1 Arg194Trp, XRCC1 Arg399Gln, and XPD Lys751Gln polymorphisms are concerned. However, there were notable differences between the groups in terms of APE1 Asp148Glu polymorphism. Associated with this condition, it has been noted that both mutant allele (Glu) frequency (37.72 % in the study group; 19.23% in the control group, p=0.0001) and homozygous mutant genotype (Glu/Glu) frequency (%12.28 in the study group; %6.60 in the control group, p=0.015) have been higher in the study group.
  • Küçük Resim Yok
    Öğe
    Micronucleus frequency in peripheral blood lymphocytes and exfoliated buccal cells of untreated cancer patients.
    (2006) Yildirim I.H.; Yesilada E.; Yologlu S.
    Some genetic diseases may increase the cellular instability. Since most human tumors have some genetic base, this study was undertaken for the genetic instability in cancer patients by micronucleus analysis, a mutation-screening test, which is more practical and economic technique than metaphase analysis carried out for chromosomal aberrations. Genetic changes were assessed in untreated cancer patients (lung, stomach and colon cancer) by different genotoxical screening methods; the cytokinesis-block micronucleus test and the buccal mucosa cell micronucleus test. The evaluation of micronuclei number in peripheral blood lymphocytes and buccal cells showed a genomic instability in somatic cells. There was a significant increase in the number of micronuclei in cancer patients prior to the initiation of chemotherapy, and/or radiotherapy compared with healthy human subjects. Furthermore, there was no significant difference between smokers and non-smoking groups or male and female groups. These results suggest that cancer in humans is characterized by an increase of chromosomal damage and thus, the micronucleus assay carried out here may be useful in routine cytogenetic studies of cancer.
  • Küçük Resim Yok
    Öğe
    Protective effect of myricetin against e2-induced genotoxic damage in human lymphocytes
    (2012) Yuksel S.; Yesilada E.; Gulbay G.; Kurtoglu E.; Serap Savaci S.
    Protective effect of myricetin (MRY) against the cytotoxic and genotoxic effects of a hormonal steroid, 17 ßestradiol (E2), was assessed in peripheral blood human lymphocyte culture. Sister chromatid exchanges (SCE), mitotic index (MI) and replication index (RI) were scored as genetic endpoints. Firstly, the genotoxic effect of different amounts (5, 10 and 20 ?M final concentration) of E2 was tested, and 10 and 20 ?M E2 levels were detected as genotoxic. In the second set, E2 groups were treated with 10 ?M MRY. MRY reduced the SCE but increased MI as well as RI, suggesting its protective action on human lymphocytes in vitro against E2-induced genotoxic damage. © by PSP.