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Öğe Comparison of biochemical, antimicrobial and cytotoxic activities of different propolis samples from Malatya and Bilecik(Syscom 18 SRL, 2020) Keskin M.; Karlidag S.; Yilmaz O.; Tatlici E.; Karabulut E.; Apohan E.; Yesilada O.Propolis is a resinous natural mixtures collected and produced by honey bees. It is rich in essential oils and phenolic components so it has high levels of antioxidant, antimicrobial, anti-inflammatory and anti-tumoral activity. In this study the biochemical activity of propolis extracts were determined. The antimicrobial activity and cytotoxic activity of the extracts of the nine different propolis samples were invastigated. Their antimicrobial activities were tested by microdillution metod and define as minimum inhibitory concentration (MIC). Chemical composition of extracts was determined by using GC-MS equipment. Total phenolic content and antioxidant activity of the extracts was measured. Antimicrobial and cytotoxic activity of the extracts was carried out as well. All of the extracts showed antimicrobial activity on bacteria and yeasts used. Extracts had generally lower MIC values on yeasts. Therefore, yeasts were detected as more susceptible against the propolis extracts than the bacteria. Cytotoxic activity of extract were determined aganist A549 and Beas2B cell lines and IC50 values were calculated. Ma-Arapgir had the highest cytotoxic activity on A549 and Beas2B. They were determined as 6.72 and 26.44 mg/mL, respectively. It could be concluded that propolis extracts have antimicrobial and cytotoxic activity thus, propolis could be used in the treatment of cancer. © 2020 SYSCOM 18 S.R.L.. All rights reserved.Öğe Enhancement of laccase production of pre-grown fungal pellets in wastewater of olive oil mills(2011) Apohan E.; Yesilada O.Previous works reported lacease production ability of batch cultures of white rot fungi in olive oil mill wastewater media (OOMW). Therefore, here, lacease production ability of repeated-batch cultures of Funalia trogii and Trametes versicolor pre-grown pellets in olive oil mill wastewater media (OOMW) was investigated and conditions for lacease production were optimized. The results indicated that high amounts of lacease could be obtained by using the best production conditions such as agitation, initial pellet amounts, initial pH, OOMW concentration and temperature. All these conditions were found to be important for lacease production. After optimization, the effect of various supplementary sources and inducer (glucose, malt extract, cheese whey and copper) on lacease production ability of these fungi and longevity of this production ability were examined in repeated-batch process. Presence of glucose, malt extract, cheese whey and copper positively affected the lacease production ability and longevity of the production. The fungal pellets also partially bioremediated the OOMW during lacease production and F. trogii pellets removed 33% COD from OOMW effluent whilst T. versicolor removed 28% in the first use. © by PSP.Öğe Valorization and biodecolorization of dye adsorbed on lignocellulosics using white rot fungi(North Carolina State University, 2012) Ozmen N.; Yesilada O.Biosorption of dyes by lignocelluloses may be an effective method for removing dyes from textile effluents. However, the resulting dyeadsorbed lignocellulosic materials may constitute another pollution problem. An integrated method can solve this problem. Here, various lignocelluloses were tested for their Astrazon Black and Astrazon Blue dyes removal activities. The dye adsorbed after 30 min contact time was 90% (45 mg/L), 70% (35 mg/L), and 98% (49 mg/L) for wheat bran, pine cone, and cotton stalk, respectively. These dye-adsorbed lignocellulosic wastes then were used as solid substrates to produce laccase enzyme with Funalia trogii and Trametes versicolor under solid state fermentation (SSF). Among the lignocellulosic substrates, the dye-adsorbed wheat bran served as the best solid substrate for laccase production under SSF. Therefore, it was also tested as a solid source for laccase production under submerged fermentation. During solid state fermentation, these two fungi were able to highly decolorize these dyes. While F. trogii decolorized 80% of Astrazon Black dye adsorbed onto wheat bran, T. versicolor decolorized 86%. On the other hand, the decolorization values for Astrazon Blue dye were 69% and 84%, respectively.
