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    Development and validation of an enzyme-linked immunosorbent assay for detection of cortisol in human saliva
    (Taylor and Francis Inc., 2017) Ozgocer T.; Yildiz S.; Uçar C.
    Non-invasive measurement of cortisol in saliva is of prime importance as it represents a bioavailable neuroendocrine marker for stress. Therefore, in this study, we developed an enzyme immune assay that was suitable for salivary cortisol measurements. For that purpose, rabbit polyclonal antibody was raised against cortisol-3-CMO:BSA conjugate. The test was based on competition of liquid phase cortisol with conjugated cortisol on the solid phase. Primary antibody was used to bind available sites on the conjugate, which was proportional to numbers of cortisol in liquid phase. Biotinylated secondary anti-rabbit antibody was used to detect primary antibodies by addition of streptavidin peroxidase and substrate, respectively. Color formation was stopped and yellow color was read by a plate-reader spectrophotometer. Additionally, validated test was used to met all validation criteria including. Test developed was used to establish cortisol awakening response (CAR) in saliva samples collected in the morning after awakening (0, 15, 30, and 60th min) from women (n = 4) and men (n = 4) at 8 or 4 different days, respectively. Diurnal cortisol levels were assessed (n = 8) at after awaking 60 min at morning, 12:00, 19:00, and 22:00 hr. In conclusion, an enzyme immunoassay test was successfully produced, validated and used for cortisol measurement in saliva samples. © 2017 Taylor & Francis.
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    Menstrual parameters in the graduate students undertaking mental or physical activity based education
    (Refik Saydam National Public Health Agency (RSNPHA), 2021) UĞRAŞ S.; Yildiz S.
    Objective: Mental activity and physical activity appears to affect body functions differently. Both activities appear to have impact on reproductive functions of the women. Aim of the current study was to compare the students undertaking mental activity based education (MABE) and physical activity based education (PABE) on menstrual cycle parameters. Methods: A total of 390 female students participated to the study. However, the students who were using any drugs (painkiller, contraceptives, etc.) were removed and the groups consisted of 171 MABE students (faculty of medicine) and 169 PABE students (faculty of sports sciences). Participants were asked to provide information about their menstrual cycles, sleep quality, pain perception. Moreover, they filled in a questionnaire about their preferences for visual, verbal, gustatory, mental, auditory, and physical activities. Results: Length of the menstrual cycle was similar between the MABE and PABE (29.5±0.3, 29.0±0.2 days, respectively, p>0.05) but length of menstruation was longer in MABE students (6.0±0.1 and 5.5±0.1 days, p=0.007). MABE students fall asleep quicker in the night, slept one h less and woke up earlier with better sleep quality. PABE students wanted to do shopping and have their hair cut and dyed while MABE students wanted to chat with a friend or make a voyage. Conclusion: Longer menstrual bleeding in MABE students requires special attention as it may result in iron deficiency anemia. Moreover, different physiological (menstruation, sleep-wake cycle) characteristics and everyday life priorities suggest that format of education and social activities of female students might require differential approaches for each education types. © 2021. All rights reserved