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Öğe Alpha lipoic acid decreases neuronal damage on brain tissue of STZ-induced diabetic rats(Pergamon-Elsevier Science Ltd, 2022) Tanbek, Kevser; Ozerol, Elif; Yilmaz, Umit; Yilmaz, Nesibe; Gul, Mehmet; Colak, CemilNeuropathy that develops due to diabetic complications causes cognitive impairment due to functional and structural damage. The aim of this study was to evaluate the biochemical, histological and physiological effects of Alpha Lipoic Acid (ALA) against brain tissue damage caused by diabetes. Fourty male Wistar albino rats were separated into four groups as control, diabetes mellitus (DM), ALA and DM+ALA. Single dose of 50 mg/kg intraperitonal streptozotocin (STZ) was used to induce DM. For six weeks, ALA (100 mg/kg/day) was administered to the ALA and DM+ALA groups. At the end of the six week rats were sacrificed by collecting blood samples and collected brain tissues (hippocampus, cortex, hippotalamus and striatum) were histologically evaluated in addition to the oxidant-antioxidant parameters. ALA administration showed significant improvement in cognitive functions evaluated by MWM in rats with diabetes mellitus (p < 0.05). SOD, CAT, GSH-Px activities, which were decreased in the DM group compared to the control group, increased statistically significantly in rats in DM+ALA group (p < 0.05). While MDA and PC levels increased in the DM group, they decreased statistically significantly in the DM+ALA group (p < 0.05). According to the histological examinations made by light and electron microscopies, it was determined that the ultrastructural damage and degeneration findings observed in the sections of the DM group were significantly ameliorated in the sections of rats in the DM+ALA group. ALA may be effective in restoring cell damage and cognitive functions in brain tissue with its antioxidant and neuroprotective effects without showing antidiabetic effects.Öğe APC Gene Expression Levels in Tumor and Adjacent Normal Tissues of Colorectal Cancer Patients(Wiley, 2017) Yilmaz, Nesibe; Yilmaz, Umit; Ergen, Arzu; Aksakal, Nihat; Zeybek, Umit[Abstract Not Available]Öğe Apc gene expression levels in tumor and adjacent normal tissues of colorectal cancer patients(Wıley, 111 rıver st, hoboken 07030-5774, nj usa, 2017) Yilmaz, Nesibe; Yilmaz, Umit; Ergen, Arzu; Aksakal, Nihat; Zeybek, UmitÖğe A case-control study on effects of the ATM, RAD51 and TP73 genetic variants on colorectal cancer risk(Walter De Gruyter Gmbh, 2019) Yazici, Merve; Yilmaz, Umit; Yilmaz, Nesibe; Celik, Faruk; Isikoren, Ece Gizem; Celikel, Burcu; Ergen, ArzuAim: ATM, RAD51 and TP73 are genes that take part in DNA repair pathways. The aim of this prospective case-control study was to determine the genotype and allele distributions of the ATM 5'-UTR G/A, RAD51 135 G/C and TP73 GC/AT polymorphisms and their relationship with clinical parameters in Turkish colorectal cancer (CRC) patients. Material and methods: One hundred and four CRC patients and 113 healthy individuals were included in this study as control. The polymerase chain reaction-restriction fragment length polymorphism techniques were used. Results: The ATM 5'-UTR G/A polymorphism GG (p = 0.001) and AA (p = 0.0001) genotypes were found higher in the patient group, while the GA genotype (p = 0.0001) and A allele (p= 0.001) were significantly higher in the control group. Moreover, the GG genotype (p = 0.042) was higher among patients with advanced-stage cancer and, while GA genotype (p = 0.047) was increased in patients without perineural invasion. The RAD51 135 G/C polymorphism GC genotype (p= 0.0001) and C allele (p = 0.0001) were significantly higher in the patient group, while CC genotype (p= 0.0001) was higher in the control group. No statistical significance was observed between the TP73 GC/AT polymorphism genotype and allele distribution and the clinical parameters. Conclusion: In the Turkish population, the ATM 5'-UTR GG and AA genotypes, and the RAD51 135 G/C GC genotype and the C allele presence may be risk factors for CRC.Öğe Detailed Anatomical Analysis of the Sphenoid Sinus and Sphenoid Sinus Ostium by Cone-Beam Computed Tomography(Lippincott Williams & Wilkins, 2016) Yilmaz, Nesibe; Kose, Evren; Dedeoglu, Numan; Colak, Cemil; Ozbag, Davut; Durak, Mehmet AkifThe aim of this study is the evaluation of the anatomical structures of sphenoid sinus ostium used as a reference point for transsphenoidal surgery by cone beam computed tomography. The authors' study was performed using the cone-beam computed tomography images of 16 to 82-year old 200 (112 female, 88 male) patients (Newton 5G, Verona, Italy). Septum deviation of sphenoid sinus and the distance between 2 ostia were evaluated by coronal and axial sections, respectively. Pneumatization degree of sphenoid sinus, diameter of sphenoid sinus ostium, and distance lower edge of superior turbinate to sphenoid sinus ostium were measured by using sagittal sections. The sellar type was the most common pneumatization type of sphenoid sinus in authors' study. While the C-type septum deviation was observed as the most common, T-type deviation was the least type. Sphenoid sinus ostium was bilaterally in 71.5% of individuals, and it was not found in 10% of individuals included in the study. A significant decrease was determined in diameter of the left sphenoid sinus ostium with aging. The distances between 2 sphenoid sinus ostia were 7.30 +/- 2.77mm for women and 6.09 +/- 2.58mm for men, respectively. No statistical differences were found in women and men in terms of distances between the lower edge of the right and left superior turbinate and sphenoid sinus ostium on their sides. Consequently, making detailed preoperative radiological evaluation of anatomic variations of sphenoid sinus and sphenoid sinus ostium is important in terms of guiding the surgeon in the process of a successful transsphenoidal surgery.Öğe Expression Levels of Micrornas Related to Autophaphy Pathway in Tumor and Adjacent Normal Tissues of Colorectal Cancer Patients(Wiley, 2017) Yilmaz, Umit; Yilmaz, Nesibe; Ergen, Arzu; Aksakal, Nihat; Zeybek, Umit[Abstract Not Available]Öğe Intracerebroventricular injection of spexin stimulates the hypothalamic-pituitary-testicular axis and increases the secretion of male reproductive hormones in rats(Elsevier Gmbh, 2024) Yilmaz, Nesibe; Ibrahim, Rida Zahiraldin; Yildiz, AzibeBackground: Male reproductive functions are regulated in the hypothalamic-pituitary-gonadal (HPG) axis. Any problem in this axis would lead to the deterioration of reproductive functions. The present study aimed to investigate the effects of intracerebroventricular (icv) Spexin (SPX) infusion on the HPG axis in detail. Methods: 40 Wistar albino rats were divided into four groups: control, sham, SPX 30 nmol and SPX 100 nmol (n=10). 30 nmol/1 mu l/hour SPX was administered icv to the rats in the SPX 30 nmol group for 7 days, while rats in the SPX 100 nmol group were administered 100 nmol/1 mu l/hour SPX. On the 7th day, the rats were decapitated, blood and tissue samples were collected. Serum LH, FSH and testosterone levels were determined with the ELISA method, GnRH mRNA expression level was determined in hypothalamus with the RT-PCR method. Seminiferous tubule diameter and epithelial thickness were determined with the hematoxylin-eosin staining method. Results: SPX infusion was increased GnRH mRNA expression in the hypothalamus tissue independent of the dose (p<0.05). Serum LH, FSH and testosterone levels in the SPX groups were increased when compared to the control and sham groups independent of the dose (p <0.05). Histological analysis revealed that SPX infusion did not lead to any changes in seminiferous epithelial thickness, while the tubule diameter increased in the SPX groups (p<0.05). Conclusion: The study findings demonstrated that icv SPX infusion stimulated the HPG axis and increased the secretion of male reproductive hormones.Öğe Intracerebroventricular PROK2 infusion could increase the secretion of male reproductive hormones by stimulating the HPG axis(Springer, 2024) Yilmaz, Nesibe; Yildiz, AzibeBackgroundProkineticin 2 (PROK2), an important neuropeptide that plays a key role in the neuronal migration of gonadotropin-releasing hormone (GnRH) in the hypothalamus, is known to have regulatory effects on the gonads. In the present study, the impact of intracerebroventricular (icv) PROK2 infusion on hypothalamic-pituitary-gonadal axis (HPG) hormones, testicular tissues, and sperm concentration was investigated.Methods and resultsRats were randomly divided into four groups: control, sham, PROK2 1.5 and PROK2 4.5. Rats in the PROK2 1.5 and PROK2 4.5 groups were administered 1.5 nmol and 4.5 nmol PROK2 intracerebroventricularly for 7 days via an osmotic mini pump (1 mu l/h), respectively. Rat blood serum follicle stimulating hormone (FSH), luteinizing hormone (LH) and testosterone hormone levels were determined with the ELISA method in the blood samples after 7 days of infusion. GnRH mRNA expression was determined with the RT-PCR in hypothalamus tissues. analyze Sperm concentration was determined, and testicular tissue was examined histologically with the hematoxylin-eosin staining method. It was observed that GnRH mRNA expression increased in both PROK2 infusion groups. Serum FSH, LH and testosterone hormone levels also increased in these groups. Although sperm concentration increased in PROK2 infusion groups when compared to the control and sham, the differences were not statistically significant. Testicular tissue seminiferous epithelial thickness was higher in the PROK2 groups when compared to the control and sham groups.ConclusionThe present study findings demonstrated that icv PROK2 infusion induced the HPG axis. It could be suggested that PROK2 could be a potential agent in the treatment of male infertility induced by endocrinological defects.Öğe Investigation of the Effect of Astaxanthin on Oxidative Stress in Renal Ischemia-Reperfusion Modeled Rats(Wiley, 2022) Kisaoglu, Aysegul; Kose, Evren; Yilmaz, Nesibe; Tanbek, Kevser; Yilmaz, Umit; Ozbag, Davut[Abstract Not Available]Öğe Investigation of the Relationship Between Pulmonary Function Test and Different Somatotypes(Wiley-Blackwell, 2016) Cay, Mahmut; Yilmaz, Nesibe; Senol, Deniz; Cevirgen, Furkan; Ucar, Cihat; Ozbag, Davut[Abstract Not Available]Öğe Phoenixin-14 may ameliorate testicular damage caused by torsion-detorsion by reducing oxidative stress and inflammation in prepubertal rats(Churchill Livingstone, 2024) Yilmaz, Nesibe; Hudaykuliyeva, Jemal; Gul, SemirThe present study aimed to investigate the effects of Phoenixin-14 (PNX-14) on oxidative damage, inflammatory response, histopathological variations, and serum testosterone levels in testicular tissues. Forty-eight Wistar albino prepubertal male rats were divided into 4 groups (Sham, TTD, TT +PNX +TD, TTD +PNX) (n =12). The torsion period was 2 hours and the detorsion period was 24 hours in the testicular torsion/detorsion (TD) groups. A single PNX-14 (50 mu g/kg) dose was injected into the rats in the TT +PNX TD group on the 90th minute of torsion, and it was injected into the rats in the TTD +PNX group at the beginning of detorsion. Oxidative damage in testicular tissues was determined based on superoxide dismutase (SOD), malondialdehyde (MDA), total antioxidant status (TAS) and total oxidant status (TOS), and inflammatory damage was determined based on TNF- alpha and IL -6 levels. Histopathological variations were investigated with the Periodic Acid Schiff (PAS) staining method in testicular tissues and analyzed based on Johnsen scores. Spermatogonia cells were examined immunohistochemically. Serum testosterone levels were determined with the enzyme -linked immunosorbent assay (ELISA). A significant increase in oxidative stress and inflammation parameters was determined in the TTD group when compared to the other groups (p <0.05). PNX-14 treatment led to a statistically significant decrease in these parameters and significantly repaired the TD damage in testicular tissue (p <0.05). Johnsen scoring revealed significant improvement in PNX-14 groups and an increase in spermatogonia count, supporting the biochemical findings (p <0.05). PNX-14 could be a potential therapeutic agent in testicular TD damage and further studies should be conducted to elucidate the present study findings.Öğe Protective effect of astaxanthin on testis torsion/detorsion injury through modulation of autophagy(Mre Press, 2024) Yilmaz, Nesibe; Yildiz, Azibe; Tanbek, Kevser; Kisaoglu, Aysegul; Yilmaz, Umit; Kose, EvrenA significant clinical condition known as testicular torsion leads to permanent ischemic damage to the testicular tissue and consequent loss of function in the testicles. In this study, it was aimed to evaluate the protective effects of Astaxanthin (ASTX) on testicular damage in rats with testicular torsion/detorsion in the light of biochemical and histopathological data. Spraque Dawley rats of 21 were randomly divided into three groups; sham, testicular torsion/detorsion (TTD) and astaxanthin + testicular torsion/detorsion (ASTX + TTD). TTD and ASTX + TTD groups underwent testicular torsion for 2 hours and then detorsion for 4 hours. Rats in the ASTX + TTD group were given 1 mg/kg/day astaxanthin by oral gavage for 7 days before torsion. Following the detorsion process, oxidative stress parameters and histopathological changes in testicular tissue were evaluated. Malondialdehyde (MDA) and total oxidant status (TOS) levels were significantly decreased in the ASTX group compared to the TTD group, while superoxide dismutase (SOD), glutathione (GSH) and total antioxidant status (TAS) levels were increased ( p < 0.05). Moreover, histopathological changes were significantly reduced in the group given ASTX ( p < 0.0001). It was determined that ASTX administration increased Beclin-1 immunoreactivity in ischemic testicular tissue, while decreasing caspase-3 immunoreactivity ( p < 0.0001). Our study is the first to investigate the antiautophagic and antiapoptotic properties of astaxanthin after testicular torsion/detorsion based on the close relationship of Beclin-1 and caspase-3 in ischemic tissues. Our results clearly demonstrate the protective effects of ASTX against ischemic damage in testicular tissue. In ischemic testicular tissue, ASTX contributes to the survival of cells by inducing autophagy and inhibiting the apoptosis.
