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    Apoptotic, Cytotoxic and Antimigratory Activities of Phenolic Compounds
    (Pleiades Publishing Inc, 2022) Yuce, H.; Sahin, Y.; Turkmen, N. Basak; Ozek, D. Askin; Unuvar, S.; Ciftci, O.
    The objective of this study was to evaluate the biological activities of chrysin (CRY), curcumin (CUR), and ellagic acid (EA) by comparing the anti-proliferative, anti-migration effects, and apoptotic gene expressions between the three human cancer cell lines: lung (A549), liver (HEP3B), and breast (MCF-7) compared to normal human fibroblast cell line (L929). Antiproliferative effects of certain phenolic compounds were determined by the MTS assay. Cells were treated with different concentrations of the compounds for two consecutive days. Their effect on cell migration was evaluated using the wound-healing assay. Apoptosis was evaluated by Bax, Bcl-2, Cas-3, Cas-8, Cas-9, Cas-10, CDK 2, CDK4, CDK6, CCNB1, and CCND2 gene expressions. The MTS assay showed that the compounds had antiproliferative effects on A549, HEP3B, and MCF-7 cell lines in a dose- and time-dependent manner. All three compounds also suppressed the migration of the tumor cell lines, significantly increased the levels of apoptotic gene expression, and induced apoptotic cell death. This study shows that chrysin, curcumin, and ellagic acid could be considered promising chemotherapeutic agents in the treatment of lung, liver, and breast cancers.
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    The Beneficial Effects of Resveratrol on Experimental Autoimmune Encephalomyelitis (EAE) in C57BL/6J Mouse Model
    (Pleiades Publishing Inc, 2022) Tecellioglu, M.; Turkmen, N. Basak; Ciftci, O.; Taslidere, A.; Ekmekyapar, T.; Yuce, H.; Oztanir, M. N.
    Multiple sclerosis (MS) is a disease of the central nervous system of unknown cause and limited therapeutical treatments. In this study we analyzed the effects of resveratrol (RSV), a polyphenolic compound with well-known neuroprotective effects, on neuronal damage in brain tissue caused by experimental autoimmune encephalomyelitis (EAE)-an established model of multiple sclerosis, using C57BL/6J female mice. A total of 40 C57BL/6J female mice were divided equally into four groups: control, EAE, RSV and RSV + EAE. 14 days after induction of EAE with myelin oligodendrocyte glycoprotein MOG35-55 and pertussis toxin, mice were treated via oral gavage with RSV at the doses of 20 mg/kg per day for 7 days. According to our results RSV treatment prevented oxidative stress caused by EAE via a decrease in lipid peroxidation and an increase in the elements of the antioxidant defense systems in brain tissue. The histopathological changes in caspase-3 and IL-17 activity and cytokine levels (TNF-alpha and IL-1 beta) induced by EAE in mouse brain tissue were reversed by RSV treatment. Moreover, elevated TNF-alpha and IL-1 beta levels, induced by EAE, were diminished in blood serum, and neurological deficits were reversed in EAE mice treated with RSV. Our findings suggest that RSV treatment effectively prevents oxidative, immunological, and histological changes in the brain caused by EAE and the beneficial effects of RSV are likely to result from its strong antioxidant and anti-inflammatory properties.
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    CTRPs, β3-AR signaling, and placental fibrin deposition: molecular and histopathological aspects of preterm birth
    (Taylor & Francis Ltd, 2026) Askin Ozek, D.; Melekoglu, R.; Akpolat, N.; Yuce, H.; Zeyveli-Celik, N.; Yasar, S.; Berberoglu, Y.
    It has been suggested that adipokines may modulate plasma lipid levels, and beta 3-adrenergic receptor (beta 3-AR) gene expressions may affect adipokine levels and play critical roles in lipid metabolism. This study aims to determine predictive biomarkers for preterm birth (PTB) by evaluating serum complement 1q (C1q)/tumor necrosis factor (TNF)-related protein (CTRP) levels, lipid profiles, gene expressions, and placental pathological changes in women experiencing PTB. A total of 80 pregnant women, 40 preterm and 40 term, who applied to the Department of Obstetrics and Gynecology, Faculty of Medicine, Inonu University, were included in the study. Blood and placenta samples were taken from all participants. Serum CTRP, high-density lipoprotein (HDL), low-density lipoprotein (LDL), total cholesterol (TC), and triglyceride (TG) levels were measured; beta 3-AR gene expression and detection rate of the beta 3-AR rs4994 (Trp64Arg) amplicon were evaluated. Placental tissues were examined histopathologically for perivillous/intervillous fibrin deposition and hydropic degeneration. ROC analysis was used to determine predictive biomarkers for PTB. In the PTB group, compared to the control group, beta 3-AR gene expression levels and serum CTRP3 levels were significantly decreased, while the detection rate of the Trp64Arg amplicon and serum CTRP4 levels were significantly increased. In addition, LDL levels increased significantly (p = 0.046), TC levels decreased (p = 0.045). According to ROC analysis, LDL (p = 0.039), TC (p = 0.034), CTRP3 (p = 0.019), and CTRP4 (p = 0.033) levels were determined as significant predictive biomarkers for PTB. Histopathological examination revealed increased perivillous and intervillous fibrin deposition and marked hydropic degeneration in the PTB group. Changes in CTRP levels, lipid profile disorders, and a decrease in beta 3-AR signaling pathways were found to be associated with PTB. LDL, TC, CTRP3, and CTRP4 levels can be evaluated as potential biomarkers that can be used in the early diagnosis and management of PTB.
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    Effect of beta-glucan on oxidative stress, inflammation, hormonal and histopathological changes in dehydroepiandrosterone-induced polycystic ovary syndrome
    (Taylor & Francis Ltd, 2026) Yuce, H.; Turkmen, N. Basak; Aydin, M.; Taslidere, A.; Ozek, D. Askin; Senkal, S.; Aslan, S.
    Beta-glucans (beta TGs) are a class of dietary fibers and biologically active polysaccharides derived from natural sources, known for their diverse bioactive properties. Their documented effects include anti-tumor, anti-inflammatory, prebiotic, anti-obesity, anti-allergic, anti-microbial, antiviral, anti-osteoporotic, and immunomodulating activities. Despite these well-established benefits, the role of beta TG in dehydroepiandrosterone (DHEA)-induced polycystic ovary syndrome (PCOS) remains largely unexplored. This study investigated the protective effects of beta TG treatment on PCOS and its potential to reverse PCOS-induced changes. Female Sprague-Dawley (SD) rats were randomly divided into four groups (n = 8 each): control, PCOS, PCOS+beta TG, and beta TG. We assessed biochemical markers related to oxidative stress, antioxidant status, inflammation, cytokines, and hormone levels. Additional analyses included immunohistochemistry and histopathology. Membrane array analysis was used to profile growth factors, cytokines, and chemokines. However, beta TG normalized deviations in the estrous cycle caused by PCOS and positively affected the reproductive system (p < 0.05). It also reduced the inflammatory response in PCOS rats by decreasing inflammatory cytokines (p < 0.05). Furthermore, oxidative stress was significantly reduced, and antioxidant enzyme activities were markedly elevated in the beta TG group (p < 0.05). Histopathological alterations were prevented by beta TG, which also induced the expression of essential proteins such as beta-nerve growth factor (bNGF), tissue inhibitor of metalloproteinase-1 (TIMP-1), Agrin, cytokine-induced neutrophil chemoattractant-1 (CINC-1), brain-derived neurotrophic factor (BDNF), and basic fibroblast growth factor (FGF-2/bFGF) (p < 0.05). In conclusion, beta TG treatment effectively protects against oxidative stress, inflammation, hormone imbalance, and histopathological damage in ovarian tissue caused by PCOS.