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Öğe A case of confined placental mosaicism with trisomy 15 associated with turner syndrome(Editions Medecine et Hygiene, 2016) Ekici C.; Sahin Y.; Yaykasli K.O.; Melekoglu R.; Sahin N.; Yuksel S.A cade ofconfined placental mosaicism with trisomy 15 associated with Turner syndrome: We here present a rare case oj'a Turner syndrome with mosaic trisomy 15 identified on chorionic villous sampling (CVS). Although there are several reports in the literature indicating confined placental mosaicism (CPM), counseling parents of a fetus with trisomy 15 mosaicism at CVS remains difficult because of the phenotypic variability. To illuminate that condition amniocentesis' or cord blood study should be offered in conjunction with genetic counseling.Öğe The effects of exogenous adrenomedullin and L-NAME on antioxidant defence system of the hypothalamic-pituitary-adrenal axis and lipid parameters of serum in rat(2011) Asma D.; Yuksel S.The aim of our study was to investigate the contribution of exogenous adrenomedullin (ADM): an antioxidant, and N sup omega nitro-L- Arginine methyl ester (L-NAME); the nitric oxide synthase (NOS) enzyme inhibitor, to balance the oxidant/antioxidant status in hypothalamus and adrenal medulla, which are both key components of the hypothalamic- Pituitary-adrenal (HPA) axis and affects some lipid metabolic mediators. Thirty six male albino SpraqueDawley rats were randomly divided into three groups: the control group, the ADM group (8 nmol/kg, i.p.), and the L-NAME group (2 ml/kg, i.p.). Measurements of tissue superoxide dismutase (SOD), catalase (CAT), glutathion S-transferase (GST) and glutathion reductase (GR) activities and glutathione (GSH) were performed in tissues using chemical protocols. The biochemical analyses for total lipid, cholesterol, triglycerides, HDL, VLDL, and LDL were conducted on an auto-analyser. Administering exogenous ADM and L-NAME caused synergic changes in the tissues, antioxidative enzyme activities, and altered serum lipoprotein profiles in rats. In the adrenal medulla and hypothalamus of the experimental groups, SOD and GST activities decreased significantly, while the CAT and GR activities increased. The amount of GSH was enhanced in the both tissues in the ADM group only, while the amount was decreased for the hypothalamus of the L-NAME group. After the treatment, the cholesterol (of L-NAME group), triglyceride, total lipid, and VLDL parameters varied significantly, but there was no significant difference between the HDL and LDL among the groups. © by PSP.Öğe Genotoxic and genoprotective effects of some antipsychotic drugs, methylphenidate and atomoxetine on human lymphocytes and HepG2 cells(Verduci Editore s.r.l, 2024) Sezer S.K.; Yuksel S.; Ucuz I.OBJECTIVE: Aripiprazole, risperidone, atomoxetine, and methylphenidate are drugs commonly prescribed for many psychiatric conditions and can be used alone or in combination in children and adolescents. This study aimed to investigate comparatively the possible genotoxic effects or genoprotective potentials of these drugs on human lymphocytes and HepG2 cells. MATERIALS AND METHODS: Cytotoxicity analysis was performed with the cell viability test on human lymphocytes and HepG2 cells, and half-maximal inhibitory concentration (IC50) values of the drugs were determined, and three different doses (IC50, IC50, and IC50) were applied for genetic analysis. For the determined doses, cells with and without DNA damage were examined by comet analysis. RESULTS: In lymphocytes, aripiprazole and risperidone increased DNA damage at moderate and maximum doses, whereas atomoxetine increased DNA damage only at the maximum dose. In HepG2 cells, risperidone reduced DNA damage at all doses, while atomoxetine increased DNA damage at all doses. On the other hand, in the DNA-damaged cells induced by hydrogen peroxide (H2O2), DNA damage decreased at all concentrations of all drugs in both lymphocytes and HepG2 cells. CONCLUSIONS: As a result, the genotoxicity of the drugs was found to be dose-dependent, and all drugs showed a genoprotective effect on DNA-damaged cells. © 2024 Verduci Editore s.r.l. All rights reserved.Öğe Genotoxic effects of tacrolimus on human lymphocyte cells.(2012) Kurtoglu E.L.; Yuksel S.We designed in vitro study to determine possible genotoxic effects oftacrolimus (FK-506), which is used as a potent immunosuppressive drug, by using sister chromatid exchange (SCEs), chromosome aberration (CAs), micronuclei tests (MN) and cell growth kinetics such as mitotic index (MI) and replication index (RI) in human lymphocytes. The cells were treated with 5, 25, 50, and 100 ng/mL concentrations of tacrolimus, for 24 h and 48 h treatment periods. Tacrolimus induced CA and MN frequency at all concentrations for 24 and 48 h In additon, it induced the SCE at the highest concantration for 24 h and at 25 and 100 ng/mL for 48 h. Tacrolimus decreased MI at all concentrations (except 5 ng/mL) for all treatment periods. It also inhibited the RI at 50 and 100 ng/mL concentrations for 24 h and at all concentrations for 48 h. Treatments given with tacrolimus result in the enhance of the different endpoints ofgenotoxicity, suggesting its mutagenic action on lymphocytes in vitro.Öğe Protective effect of extracts of teucrium polium and rumex crispus against cyclophosphamide-induced genotoxic damage in human lymphocytes(Eco-Vector LLC, 2019) Yuksel S.; Sezer S.K.; Kurtoglu E.L.; Bag H.G.Teucrium polium (T. polium) and Rumex crispus (R. crispus) are plant species that grow widely in Anatolia and are thought to have healing effects for many diseases. In this study plant extracts are suggested as alternative agents in repairing cellular damage by using sister chromatid exchange (SCE), micronucleus (MN), mitotic index (MI), replication index (RI) and nuclear abnormalities (NAs), against the genotoxicity of cyclophosphamide (CP) in the human lymphocyte cells. 8 experimental groups were formed in the study. The cell culture medium was supplemented with 0.16 ?g/ml CP and the cells were treated with 50, 100 and 250 ?M T. polium and R. crispus extracts in the presence and absence of CP. As a result, CP significantly decreased MI frequency while increasing SCE, MN and NAs frequencies in cells. 100 ?M T. polium plus CP decreased SCEs when compared with CP alone. In addition, MN frequency was significantly decreased in 100 ?M T. polium plus CP and 250 ?M R. crispus plus CP combine groups. Our results suggest that these plant extracts are not genetically damaging and have improving effects at these doses. © 2019 Eco-Vector LLC. All rights reserved.Öğe Protective effect of myricetin against e2-induced genotoxic damage in human lymphocytes(2012) Yuksel S.; Yesilada E.; Gulbay G.; Kurtoglu E.; Serap Savaci S.Protective effect of myricetin (MRY) against the cytotoxic and genotoxic effects of a hormonal steroid, 17 ßestradiol (E2), was assessed in peripheral blood human lymphocyte culture. Sister chromatid exchanges (SCE), mitotic index (MI) and replication index (RI) were scored as genetic endpoints. Firstly, the genotoxic effect of different amounts (5, 10 and 20 ?M final concentration) of E2 was tested, and 10 and 20 ?M E2 levels were detected as genotoxic. In the second set, E2 groups were treated with 10 ?M MRY. MRY reduced the SCE but increased MI as well as RI, suggesting its protective action on human lymphocytes in vitro against E2-induced genotoxic damage. © by PSP.
