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    Identification of bacterial species in milk by MALDI-TOF and assessment of some oxidant-antioxidant parameters in blood and milk from cows with different health status of the udder
    (Polska Akad Nauk, Polish Acad Sciences, Univ Warmia & Mazury Olsztyn, 2022) Ozbey, G.; Cambay, Z.; Yilmaz, S.; Aytekin, O.; Zigo, F.; Ozcelik, M.; Otlu, B.
    This study aimed to identify bacterial pathogens in milk samples from dairy cows with sub -clinical and clinical mastitis as well as to assess the concentrations of oxidant-antioxidant param-eters [malondialdehyde (MDA), reduced glutathione (GSH), and total GSH levels] in both blood and milk samples. From a total of 200 dairy cows in 8 farms, 800 quarter milk samples obtained from each udder were tested in the laboratory for the presence of udder pathogens. Cultivated bacteria causing intramammary infection from milk samples were identified by Matrix-Assisted Laser Desorption/Ionization-Time of Flight (MALDI-TOF). In addition, from tested animals 60 cows were selected includ??ng 20 healthy cows that were CMT negative, 20 cows with subclini-cal mastitis (SM), and 20 cows with clinical mastitis (CM) for detection of MDA, GSH, and total GSH levels in blood and milk samples. Three hundred and eighty (47.5%; 380/800), 300 (37.5%; 300/800), and 120 (15%; 120/800) of milk samples, respectively were CMT positive or SM and CM, and those positives were cows from different farms. We observed that 87.4% (332/380), 25.3% (76/300), and 34.2% (41/120) of cows with CMT positive, CMT negative, and CM had bacterial growth. The most predominantly identified bacteria were Staphylococcus chromogenes (18.7%) obtained mainly from SM and Staphylococcus aureus (16.7%) as the most frequent cau-se of CM. According to our results, dairy cows with CM had the highest MDA levels, the lowest GSH, and total GSH levels in both blood and milk samples however, high MDA levels and low GSH levels in milk samples with SM were observed. Based on our results, lipid oxidant MDA and antioxidant GSH could be excellent biomarkers of cow???s milk for developing inflammation of the mammary gland. In addition, there was no link between nutrition and MDA and GSH levels.
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    Investigation of Antimicrobial Activities and Molecular Docking Studies of Synthesized Sulfonamide Compounds
    (Springer, 2023) Ozbey, G.; Tanriverdi, E. S.; Senkal, B. F.; Korkmaz, B.; Erkan, S.; Bulut, N.; Zigo, F.
    Sulfonamides are commonly used worldwide. In this study, several sulfonamide compounds such as N-(4-acetylphenyl)-4-methylbenzenesulfonamide (PSASF), N-(3-acetylphenyl)-4-methylbenzenesulfonamide (PSASF-1), 1-tosyl-1H-imidazole (PSASF-x), 4-methyl-N-(pyridin-4-yl) benzenesulfonamide (PSASF-2), and 1-ethyl-4-tosylpiperazine (PSASF-3) have been synthesized, with antibacterial activities against Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, and Staphylococcus aureus ATCC 29213 have been evaluated. Antibacterial properties of drugs were studied in depth using molecular docking research. In addition, the synthesized compounds were characterized using spectral analysis. Antibacterial activities of synthesized derivates were determined against E. coli, P. aeruginosa, and S. aureus with minimum inhibitory concentration (MIC) by using the broth microdilution method. All prepared compounds exhibited significant antibacterial activity against S. aureus, E. coli, and P. aeruginosa. The MIC value for E. coli and P. aeruginosa was determined as 256 mu g/mL. MIC against S. aureus was observed to be 256 and 512 mu g/mL for the PSASF compound and the other compounds respectively. Results of the current study revealed that four of the five compounds had weaker antibacterial activity against S. aureus at a concentration of 512 mu g/mL. However, the MIC values from our experiments are significantly higher in comparison with the reference drugs such as amoxicillin, ciprofloxacin, meropenem, and vancomycin in E. coli and S. aureus. On the other hand, in a comparison of the synthesized compounds with reference drugs in P. aeruginosa, no statistical difference was demonstrated. Antibacterial activity of the produced derivates was likewise an agreement with regard to the molecular docking and the laboratory results.