Cloning and Expression vgb-kfo Genes in E. coli and Microbial Chondroitin Sulfate Production

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dc.authoridAKPOLAT, Nusret/0000-0002-9138-2117
dc.authoridGeckil, Hikmet/0000-0003-0070-0691
dc.authorwosidAKPOLAT, Nusret/ABF-6986-2020
dc.authorwosidGeckil, Hikmet/F-7647-2012
dc.contributor.authorErenler, Ayse Sebnem
dc.contributor.authorGeckil, Hikmet
dc.contributor.authorKarabulut, Aysun Bay
dc.contributor.authorAkpolat, Nusret
dc.contributor.authorSevimli, Resit
dc.contributor.authorUlke, Esra
dc.contributor.authorAliyeva, Aygun
dc.date.accessioned2024-08-04T20:57:26Z
dc.date.available2024-08-04T20:57:26Z
dc.date.issued2019
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThis study focused on a new plasmid and new recombinant strain developed for the production of microbial chondroitin sulfate a new and limited area of study the strategies we applied for the production of microbial chondroitin sulfate, and the possible contributions of this study to published research literature. In this study, pETM6-PACF, which carries the genes responsible for capsular chondroitin synthesis [kfA, kfoC, kfoF] was used as the basic plasmid. The Vitreoscilla hemoglobin gene region was transformed into this basic plasmid and the common expression of both gene groups was added to research literature for the first time. This plasmid was transferred to non-pathogenic E. coil (C2987) to produce a completely new chondroitin source specific to this study. Following the transformation by chondroitin synthesis, and the subsequent microbial production of chondroitin by the application of purification protocols, microbial chondroitin sulfate was produced in sulfate form. Consequently, in comparison to published literature, a product with a low molecular weight value of 269 Daltons was developed. This product, which has significant potential drug potency, can be used in many different areas as a novel and unique biomedical product.en_US
dc.description.sponsorshipTUBITAK 1001 [215S-861]en_US
dc.description.sponsorshipThis study was supported by TUBITAK 1001 (215S-861).en_US
dc.identifier.doi10.1166/sam.2019.3693
dc.identifier.endpage1754en_US
dc.identifier.issn1947-2935
dc.identifier.issn1947-2943
dc.identifier.issue12en_US
dc.identifier.startpage1745en_US
dc.identifier.urihttps://doi.org/10.1166/sam.2019.3693
dc.identifier.urihttps://hdl.handle.net/11616/102611
dc.identifier.volume11en_US
dc.identifier.wosWOS:000490289600012en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherAmer Scientific Publishersen_US
dc.relation.ispartofScience of Advanced Materialsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectChondroitinen_US
dc.subjectChondroitin Sulfateen_US
dc.subjectVitreoscilla Hemoglobinen_US
dc.subjectBacterial Hemoglobinen_US
dc.titleCloning and Expression vgb-kfo Genes in E. coli and Microbial Chondroitin Sulfate Productionen_US
dc.typeArticleen_US

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