Clinical Strains of Chryseobacterium and Elizabethkingia spp. Isolated from Pediatric Patients in a University Hospital: Performance of MALDI-TOF MS-Based Identification, Antimicrobial Susceptibilities, and Baseline Patient Characteristics

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dc.authoridBüyükcam, Ayşe/0000-0002-4015-4210
dc.authoridTugcu, Gokcen Dilsa/0000-0002-9804-1200
dc.authoridOZCELIK, HAYRIYE UGUR/0000-0003-1587-5216
dc.authoridKARA, ATES/0000-0002-1654-3232
dc.authoridOTLU, BARIS/0000-0002-6220-0521
dc.authoridMirza, Hasan Cenk/0000-0002-8853-3893
dc.authorwosidBüyükcam, Ayşe/AAV-6986-2020
dc.authorwosidOLMEZ, SERPIL/I-8374-2013
dc.authorwosidTugcu, Gokcen Dilsa/ABG-5531-2021
dc.authorwosidSENER, BURCIN/I-8362-2013
dc.authorwosidOZCELIK, HAYRIYE UGUR/I-9320-2013
dc.authorwosidKARA, ATES/I-9081-2013
dc.authorwosidOTLU, BARIS/ABI-5532-2020
dc.contributor.authorMirza, Hasan Cenk
dc.contributor.authorTuncer, Ozlem
dc.contributor.authorOlmez, Serpil
dc.contributor.authorSener, Burcin
dc.contributor.authorTugcu, Gokcen Dilsa
dc.contributor.authorOzcelik, Ugur
dc.contributor.authorGursoy, Nafia Canan
dc.date.accessioned2024-08-04T20:57:26Z
dc.date.available2024-08-04T20:57:26Z
dc.date.issued2018
dc.departmentİnönü Üniversitesien_US
dc.description.abstractOur objective was to evaluate the performance of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for identification of the Chryseobacterium and Elizabethkingia spp. isolated from pediatric patients at Hacettepe University Hospital using 16S rRNA gene sequencing as the gold standard and to determine the antimicrobial susceptibility patterns of the isolates and baseline characteristics of patients. All stored Chryseobacterium and Elizabethkingia spp. isolated from various clinical specimens (sputum, blood, and urine) of pediatric patients at Hacettepe University Hospital between 2012 and 2016 were included in this study. Minimum inhibitory concentrations of 10 antimicrobial agents were determined by Etest for all isolates. To determine the baseline characteristics of patients, medical records of all patients were retrospectively reviewed. In total, 18 isolates of Chryseobacterium spp. (16 C. indologenes, 2 C. gleum) and 5 isolates of Elizabethkingia spp. (3 E. meningoseptica, 2 E. anophelis) were identified by 16S rRNA sequencing. MALDI-TOF MS correctly identified 19 (82.6%) isolates to the species level. The quinolones (ciprofloxacin and levofloxacin), trimethoprim/sulfamethoxazole and piperacillin/tazobactam showed the highest spectrum of activity against the overall collection of isolates. Cystic fibrosis (CF) was the underlying disease in 81.8% of patients. To our knowledge, this study includes the largest number of Chryseobacterium spp. isolated from clinical specimens of pediatric patients in Turkey. In this study, we also report the first clinical isolate of E. anophelis in Turkey. Since, the majority of strains were isolated from patients with CF; larger, prospective clinical studies are needed to establish whether chryseobacteria could be considered as an emerging opportunistic pathogen in patients with CF.en_US
dc.identifier.doi10.1089/mdr.2017.0206
dc.identifier.endpage821en_US
dc.identifier.issn1076-6294
dc.identifier.issn1931-8448
dc.identifier.issue6en_US
dc.identifier.pmid29227188en_US
dc.identifier.startpage816en_US
dc.identifier.urihttps://doi.org/10.1089/mdr.2017.0206
dc.identifier.urihttps://hdl.handle.net/11616/102608
dc.identifier.volume24en_US
dc.identifier.wosWOS:000417569600001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMary Ann Liebert, Incen_US
dc.relation.ispartofMicrobial Drug Resistanceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectChryseobacterium spp.en_US
dc.subjectElizabethkingia spp.en_US
dc.subjectE. anophelisen_US
dc.subjectMALDI-TOF MSen_US
dc.subjectantimicrobial resistanceen_US
dc.subjectcystic fibrosisen_US
dc.titleClinical Strains of Chryseobacterium and Elizabethkingia spp. Isolated from Pediatric Patients in a University Hospital: Performance of MALDI-TOF MS-Based Identification, Antimicrobial Susceptibilities, and Baseline Patient Characteristicsen_US
dc.typeArticleen_US

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