Mystery of Immune Response in Relapsed Brucellosis: Immunophenotyping and Multiple Cytokine Analysis

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dc.authoridKURTOĞLU, Elçin Latife/0000-0002-8375-8399
dc.authoridGül, Mehmet/0000-0002-1374-0783
dc.authoridOTLU, BARIS/0000-0002-6220-0521
dc.authoridKAYHAN, BASAK/0000-0003-3508-2563
dc.authoridKayabas, Uner/0000-0002-5323-0796
dc.authoridBayindir, Yasar/0000-0003-3930-774X
dc.authorwosidKURTOĞLU, Elçin Latife/AAQ-6842-2020
dc.authorwosidGül, Mehmet/ABI-6336-2020
dc.authorwosidOTLU, BARIS/ABI-5532-2020
dc.authorwosidKAYHAN, BASAK/ABH-9314-2020
dc.authorwosidKayabas, Uner/JRX-1616-2023
dc.authorwosidASLAN, Elçin Latife/A-3923-2017
dc.authorwosidBayindir, Yasar/T-1523-2017
dc.contributor.authorKayhan, Basak
dc.contributor.authorKayabas, Uner
dc.contributor.authorKolgelier, Servet
dc.contributor.authorOtlu, Baris
dc.contributor.authorGul, Mehmet
dc.contributor.authorKurtoglu, Elcin Latife
dc.contributor.authorBayindir, Yasar
dc.date.accessioned2024-08-04T21:00:02Z
dc.date.available2024-08-04T21:00:02Z
dc.date.issued2016
dc.departmentİnönü Üniversitesien_US
dc.description.abstractIntroduction: Brucella spp. are intracellular bacteria that may cause acute, subacute and chronic infections. Despite optimum antibiotic treatment, relapse of brucellosis occurs in some patients. There is less amount of knowledge about immune response in relapse of brucellosis. Materials and Methods: Twenty patients with acute brucellosis, 16 patients with relapsed brucellosis and as a control group 20 healthy volunteers were enrolled in this study to explore the immune response variation during relapse of brucellosis. The distribution of peripheral blood mononuclear cells was investigated by flow cytometry and level of various cytokines involved in inflammatory and anti-inflammatory response were measured by enzyme-linked immunosorbent assay in serum samples. Results: The most prominent data in phenotyping examination was the significant reduction (1.45 times) in the percentage of activated T cell (CD3(+) human leukocyte antigen-DR+) population in the relapse group in comparison to the acute brucellosis group. However, percentage of activated T cell population in the relapse group was 2.59 times higher than in healthy controls (p< 0.01). We observed a significant reduction in inflammatory cytokines interleukin (IL)-6, IL-18, interferon-gamma and IL-17 in relapsed patients in comparison to patients with acute brucellosis. While there was no significant difference in IL-15 and tumor necrosis factor-alpha levels between relapse and acute brucellosis groups, the levels of these two cytokines were significantly higher in the relapse group than in healthy subjects. In case of anti-inflammatory cytokines, while IL-4 levels increased significantly only in relapse group, IL-10 levels increased both in acute and relapse brucellosis group in comparison to healthy controls. Interestingly, we observed 2.87 times elevation in IL-4 levels in the relapse group in comparison to acute brucellosis (p< 0.01). Similarly; IL-10 levels increased 2.09 times in patients with relapsed brucellosis patients in comparison to acute brucellosis (p< 0.01). Conclusion: Elevation of regulatory cytokines in systemic immune system and reduction of activated T cell frequency occur during the relapse of brucellosis. These results may contribute to understanding the immunopathology in the systemic circulation during relapse of brucellosis.en_US
dc.description.sponsorshipInonu University [2011/176]en_US
dc.description.sponsorshipThis study was granted by Inonu University Scientific Investigation Fund-2011/176.en_US
dc.identifier.doi10.4274/mjima.2016.2
dc.identifier.issn2147-673X
dc.identifier.urihttps://doi.org/10.4274/mjima.2016.2
dc.identifier.urihttps://hdl.handle.net/11616/103735
dc.identifier.volume5en_US
dc.identifier.wosWOS:000407294300002en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherGalenos Yayinciliken_US
dc.relation.ispartofMediterranean Journal of Infection Microbes and Antimicrobialsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBrucellosisen_US
dc.subjectcytokinesen_US
dc.subjectimmune responseen_US
dc.subjectrelapseen_US
dc.subjecttumor necrosis factor alphaen_US
dc.titleMystery of Immune Response in Relapsed Brucellosis: Immunophenotyping and Multiple Cytokine Analysisen_US
dc.typeArticleen_US

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