Absence of superoxide dismutase activity causes nuclear DNA fragmentation during the aging process

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dc.authorid110769en_US
dc.contributor.authorMuid, Khandaker Ashfaqul
dc.contributor.authorKarakaya, Hüseyin Çağlar
dc.contributor.authorKoç, Ahmet
dc.date.accessioned2017-06-05T12:58:44Z
dc.date.available2017-06-05T12:58:44Z
dc.date.issued2014
dc.departmentİnönü Üniversitesien_US
dc.descriptionBiochemical and Biophysical Research Communications 444 (2014) 260–263en_US
dc.description.abstractSuperoxide dismutases (SOD) serve as an important antioxidant defense mechanism in aerobic organisms, and deletion of these genes shortens the replicative life span in the budding yeast Saccharomyces cerevisiae. Even though involvement of superoxide dismutase enzymes in ROS scavenging and the aging process has been studied extensively in different organisms, analyses of DNA damages has not been performed for replicatively old superoxide dismutase deficient cells. In this study, we investigated the roles of SOD1, SOD2 and CCS1 genes in preserving genomic integrity in replicatively old yeast cells using the single cell comet assay. We observed that extend of DNA damage was not significantly different among the young cells of wild type, sod1D and sod2D strains. However, ccs1D mutants showed a 60% higher amount of DNA damage in the young stage compared to that of the wild type cells. The aging process increased the DNA damage rates 3-fold in the wild type and more than 5-fold in sod1D, sod2D, and ccs1D mutant cells. Furthermore, ROS levels of these strains showed a similar pattern to their DNA damage contents. Thus, our results confirm that cells accumulate DNA damages during the aging process and reveal that superoxide dismutase enzymes play a substantial role in preserving the genomic integrity in this process.en_US
dc.identifier.citationKA, Muid, KARAKAYA, H. Ç., & KOÇ, A. (2014). Absence Of Superoxide Dismutase Activity Causes Nuclear DNA Fragmentation During The Aging Process. Biochemical And Biophysical Research Communications, 444(2), 260–263.en_US
dc.identifier.doi10.1016/j.bbrc.2014.01.056en_US
dc.identifier.endpage263en_US
dc.identifier.issue2en_US
dc.identifier.startpage260en_US
dc.identifier.urihttp://linkinghub.elsevier.com/retrieve/pii/S0006291X14000801
dc.identifier.urihttps://hdl.handle.net/11616/7051
dc.identifier.volume444en_US
dc.language.isoenen_US
dc.publisherBiochemical and Biophysical Research Communicationsen_US
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectOxidative stressen_US
dc.subjectAntioxidanten_US
dc.subjectSODen_US
dc.subjectSuperoxide dismutaseen_US
dc.subjectAgingen_US
dc.subjectLongevityen_US
dc.subjectDNA damageen_US
dc.subjectComet assayen_US
dc.subjectROSen_US
dc.subjectReactive oxygen speciesen_US
dc.titleAbsence of superoxide dismutase activity causes nuclear DNA fragmentation during the aging processen_US
dc.typeArticleen_US

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