Characterization of two genes encoding metal tolerance proteins from Beta vulgaris subspecies maritima that confers manganese tolerance in yeast
Yükleniyor...
Dosyalar
Tarih
2013
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
BioMetals
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Manganese (Mn2?) is an essential micronutrient
in plants. However increased Mn2? levels are
toxic to plant cells. Metal tolerance proteins (MTPs),
member of cation diffusion facilitator protein (CDF)
family, have important roles in metal homeostatis in
different plant species and catalyse efflux of excess
metal ions. In this study, we identified and characterized
two MTP genes from Beta vulgaris spp. maritima
(B. v. ssp. maritima). Overexpression of these two
genes provided Mn tolerance in yeast cells. Sequence
analyses displayed BmMTP10 and BmMTP11as
members of the Mn-CDF family. Functional analyses
of these proteins indicated that they are specific to
Mn2? with a role in reducing excess cellular Mn2?
levels when expressed in yeast. GFP-fusion constructs
of both proteins localized to the Golgi apparatus
as a punctuated pattern. Finally, Q-RT-PCR results
showed that BmMTP10 expression was induced
threefold in response to the excess Mn2? treatment.
On the other hand BmMTP11 expression was not
affected in response to excess Mn2? levels. Thus, our
results suggest that the BmMTP10 and BmMTP11
proteins from B. v. ssp. maritima have non-redundant
functions in terms of Mn2? detoxification with a
similar in planta localization and function as the
Arabidopsis Mn-CDF homolog AtMTP11 and this
conservation shows the evolutionary importance of
these vesicular proteins in heavy metal homeostatis
among plant species.
Açıklama
Biometals (2013) 26:795–804
Anahtar Kelimeler
MTP, Heavy metal tolerance, Manganese, Yeast, Golgi, Plant
Kaynak
BioMetals
WoS Q Değeri
Scopus Q Değeri
Cilt
26
Sayı
0
Künye
Erbasol Isil, Bozdag Gonensin Ozan, Koc Ahmet, Pedas Pai, Karakaya Huseyin Caglar, (2013). Characterization of two genes encoding metal tolerance proteins from Beta vulgaris subspecies maritima that confers manganese tolerance in yeast. Biometals (2013) 26:795–804.
