DETERMINATION OF TOTAL CELL PROTEIN PROFILES OF Streptomyces SPECIES

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dc.authoridatalan, ekrem/0000-0002-7986-392X
dc.authorwosidatalan, ekrem/ABI-5475-2020
dc.contributor.authorZdemir, K.
dc.contributor.authorBerber, I
dc.contributor.authorOgun, E.
dc.contributor.authorAtalan, M.
dc.date.accessioned2024-08-04T20:57:37Z
dc.date.available2024-08-04T20:57:37Z
dc.date.issued2013
dc.departmentİnönü Üniversitesien_US
dc.description.abstractPresent study has been conducted for finding out the total protein profile of bacterial strain Streptomyces sps by sodium dodecyl sulphate polyacrylamide gel electrophoresis. Total 139 isolates of Streptomyces have been isolated from the soil. Amongst all isolated strain, total 20 isolates were used for getting protein profile by SDS PAGE. Amongst all isolates, 20 isolates were selected for protein profiling and these were divided in two groups. Two strains of Streptomyces i.e. S. violaceus and S. albidoflavus were selected as a reference strain for both groups. Band profile were analyzed and assessed by computer added program BioRad Quantity with the use of Unweighted Pair Group Method of Analysis (UPGMA). As a result of this computer assisted numeric analysis study, approximately 40 different types of protein bands were reported between 10 or 100 kD molecular weight. Analysis of acquired dendogram on the basis of similarities ratios, all 40 proteins can be divided in to 7 groups. In addition, the isolates A4B3G, D145B, S5036.6 and reference isolate S. violaceus were available in the same group, while 805A, C804B, F1705 isolates and reference sample S. albidoflavus were detected in the same group. The test organisms which were similar to each other in terms of morphological and biochemical characters delivered the same protein bands. SDS-PAGE method is an effective method in terms of determining taxonomical relations between the various species of genus Streptomyces.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [TBAG-2344(103T156)]en_US
dc.description.sponsorshipThis work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) Grant Number = TBAG-2344(103T156).en_US
dc.identifier.endpage190en_US
dc.identifier.issn2320-8694
dc.identifier.issue3en_US
dc.identifier.startpage186en_US
dc.identifier.urihttps://hdl.handle.net/11616/102782
dc.identifier.volume1en_US
dc.identifier.wosWOS:000215845200006en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherJournal Experimental Biology & Agricultural Sciencesen_US
dc.relation.ispartofJournal of Experimental Biology and Agricultural Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectStreptomycesen_US
dc.subjectTotal Cell Protein Profileen_US
dc.subjectSDS-PAGEen_US
dc.subjectNumerical Analysisen_US
dc.titleDETERMINATION OF TOTAL CELL PROTEIN PROFILES OF Streptomyces SPECIESen_US
dc.typeArticleen_US

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