Examination of the effect of xenogeneic mesenchymal stem cells and conditioned medium on cartilage graft viability: a rabbit model

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dc.authoridBulbul, Volkan/0000-0003-1526-2065
dc.authoridKolbasi, Bircan/0000-0001-7933-4262
dc.authoridozdemir, ilkay/0000-0003-2715-6825
dc.authoridsutcu, mustafa/0000-0001-5106-0159
dc.authorwosidkeskin, ilknur/A-6521-2018
dc.authorwosidBulbul, Volkan/HPH-9160-2023
dc.authorwosidsutcu, mustafa/G-9012-2016
dc.contributor.authorUnlu, Abdullah
dc.contributor.authorBulbul, Muhammet Volkan
dc.contributor.authorErkan, Bircan Kolbasi
dc.contributor.authorOzdemir, Ilkay
dc.contributor.authorKeskin, Ilknur
dc.contributor.authorSutcu, Mustafa
dc.date.accessioned2024-08-04T20:58:47Z
dc.date.available2024-08-04T20:58:47Z
dc.date.issued2023
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBackground Since cartilage, unlike skin, does not contain vessels, it obtains nutrition by diffusion. This reduces graft viability, resulting in problems such as reductions in size, changes in shape, and resorption of the cartilage graft in the late post-graft period. This study aimed to investigate the effects of adipose-derived mesenchymal cells and conditioned medium (CM) on cartilage graft viability. Methods Dissections were performed 4 months after the injection of 0.5 mL of CM or 2x10(6) mesenchymal stem cells (MSCs) in 0.5 mL after grafting into a control group and two experimental groups (n=21 rabbits in total). Chondrocyte viability and type II collagen expression in the grafted areas were analyzed by hematoxylin-eosin staining and immunohistochemical methods, respectively. Results In the MSC and CM groups, chondrocyte proliferation at the graft tissue incision margin (MSC: P<0.01, CM: P<0.0001), chondrocyte proliferation at the auricular cartilage incision margin (MSC: P<0.05, CM: P<0.0001), integration of the graft with the surrounding cartilage (MSC: P<0.001, CM: P<0.0001) and type II collagen expression levels (MSC: P=0.001, CM: P=0.0002) significantly increased. Conclusions Xenogenic injection of MSCs and CM contributed to new cartilage production without any tumoral effects or immune reactions. In particular, the cell-free nature of CM strengthened its potential for safe use. Since injections of MSC and CM can preserve cartilage graft viability, interest in this technique is expected to increase as longterm results from clinical studies on the subject become available.en_US
dc.identifier.doi10.14730/aaps.2022.00773
dc.identifier.endpage135en_US
dc.identifier.issn2234-0831
dc.identifier.issn2288-9337
dc.identifier.issue3en_US
dc.identifier.startpage129en_US
dc.identifier.urihttps://doi.org/10.14730/aaps.2022.00773
dc.identifier.urihttps://hdl.handle.net/11616/103168
dc.identifier.volume29en_US
dc.identifier.wosWOS:001072175300002en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherKorean Soc Aesthetic Plastic Surgeryen_US
dc.relation.ispartofArchives of Aesthetic Plastic Surgeryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCartilageen_US
dc.subjectAdipose-derived mesenchymal stem cellsen_US
dc.subjectGraften_US
dc.subjectCollagen type 2en_US
dc.titleExamination of the effect of xenogeneic mesenchymal stem cells and conditioned medium on cartilage graft viability: a rabbit modelen_US
dc.typeArticleen_US

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