Rapid detection of bloodstream pathogens in liver transplantation patients with filmarray multiplex polymerase chain reaction assays comparison with conventional methods
| dc.authorid | 101949 | en_US |
| dc.authorid | 10690 | en_US |
| dc.authorid | 28465 | en_US |
| dc.authorid | 53189 | en_US |
| dc.authorid | 110318 | en_US |
| dc.authorid | 110105 | en_US |
| dc.authorid | 112689 | en_US |
| dc.contributor.author | Otlu, Barış | |
| dc.contributor.author | Bayındır, Yaşar | |
| dc.contributor.author | Özdemir, Fatih | |
| dc.contributor.author | İnce, Volkan | |
| dc.contributor.author | Çuğlan, Songül | |
| dc.contributor.author | Hopoğlu, Murat | |
| dc.contributor.author | Yakupoğulları, Yusuf | |
| dc.contributor.author | Kızılkaya, Cana | |
| dc.contributor.author | Kuzucu, Çiğdem | |
| dc.contributor.author | Işık, Burak | |
| dc.contributor.author | Yılmaz, Sezai | |
| dc.date.accessioned | 2017-11-08T06:57:11Z | |
| dc.date.available | 2017-11-08T06:57:11Z | |
| dc.date.issued | 2015 | |
| dc.department | İnönü Üniversitesi | en_US |
| dc.description | Transplantation Proceedings | en_US |
| dc.description.abstract | Background. Bloodstream infection (BSI) is an important concern in transplant patients. Early intervention with appropriate antimicrobial therapy is critical to better clinical outcome; however, there is significant delay when conventional identification methods are used. Methods. We aimed to determine the diagnostic performance of the FilmArray BloodCulture Identification Panel, a recently approved multiplex polymerase chain reaction assay detecting 24 BSI pathogens and 3 resistance genes, in comparison with the performances of conventional identification methods in liver transplant (LT) patients. A total of 52 defined sepsis episodes (signal-positive by blood culture systems) from 45 LT patients were prospectively studied. Results. The FilmArray successfully identified 37 of 39 (94.8%) bacterial and 3 of 3 (100%) yeast pathogens in a total of 42 samples with microbial growth, failing to detect only 2 of 39 (5.1%) bacterial pathogens that were not covered by the test panel. The FilmArray could also detect additional pathogens in 3 samples that had been reported as having monomicrobial growth, and it could detect Acinetobacter baumannii in 2 samples suspected of skin flora contamination. The remaining 8 blood cultures showing a positive signal but yielding no growth were also negative by this assay. Results of MecA, KPC, and VanA/B gene detection were in high accordance. The FilmArray produced results with significantly shorter turnaround times (1.33 versus 36.2, 23.6, and 19.5 h; P < .05) than standard identification methods, Vitek II, and Vitek MS, respectively. Conclusions. This study showed that the FilmArray appeared as a reliable alternative diagnostic method with the potential to mitigate problems with protracted diagnosis of the BSI pathogens in LT patients. | en_US |
| dc.identifier.citation | Otlu, B., Bayındır, Y., Özdemir, F., İnce, V., Çuğlan, S., Hopoğlu, M., … Yılmaz, S. (2015). Rapid Detection Of Bloodstream Pathogens İn Liver Transplantation Patients With Filmarray Multiplex Polymerase Chain Reaction Assays Comparison With Conventional Methods. Transplantation Proceedings, 47(6), 1926–1932. | en_US |
| dc.identifier.doi | 10.1016/j.transproceed.2015.02.025 | en_US |
| dc.identifier.endpage | 1932 | en_US |
| dc.identifier.issue | 6 | en_US |
| dc.identifier.startpage | 1926 | en_US |
| dc.identifier.uri | https://www.ncbi.nlm.nih.gov/pubmed/26293075 | |
| dc.identifier.uri | https://hdl.handle.net/11616/7835 | |
| dc.identifier.volume | 47 | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | Transplantation Proceedings | en_US |
| dc.relation.ispartof | Transplantation Proceedings | en_US |
| dc.rights | info:eu-repo/semantics/openAccess | en_US |
| dc.title | Rapid detection of bloodstream pathogens in liver transplantation patients with filmarray multiplex polymerase chain reaction assays comparison with conventional methods | en_US |
| dc.type | Article | en_US |
