Downregulatory effect of miR-342-3p on epileptogenesis in the PTZ-kindling model

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dc.authoridPala, Mukaddes/0000-0002-0610-0526
dc.authoridokur, sema karaca/0000-0002-0692-8155
dc.authorwosidPala, Mukaddes/HKE-7637-2023
dc.authorwosidYılmaz, Şenay Görücü/A-3797-2016
dc.contributor.authorPala, Mukaddes
dc.contributor.authorMeral, Ismail
dc.contributor.authorAcikgoz, Nilgun Pala
dc.contributor.authorYilmaz, Senay Gorucu
dc.contributor.authorOkur, Semra Karaca
dc.contributor.authorAcar, Seyma
dc.contributor.authorPolat, Yalcin
dc.date.accessioned2024-08-04T20:57:43Z
dc.date.available2024-08-04T20:57:43Z
dc.date.issued2022
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBackground Epileptogenesis is a process that results in neurons firing abnormally, causing seizures. Increasing evidence has shown that miRNAs expressed in the epileptic hippocampus are involved in epileptogenesis. We demonstrated the expression changes of miRNAs that may be effective in epileptogenesis in silico analysis in the kindling model created with Pentylenetetrazole (PTZ). Thus, we aimed to identify the target genes responsible for epileptogenesis. Methods and results Fifteen male Wistar-albino rats (200-230 g) were randomly divided into two groups control (n = 6) and PTZ (n = 9). The control group received 0.5 ml saline, and the PTZ group (35 mg/kg i.p.) intraperitoneally (i.p.) (11 times, every other day) to induce tonic-clonic seizures. Seizures were observed and scored 30 min after PTZ injection. After the last dose of PTZ (75 mg/kg) administration, the hippocampus tissues of the rats were removed by anesthesia. Analysis of miRNAs was performed with the Affymetrix gene chip miRNA sequence (728 miRNA) and confirmed by the Real-Time Polymerase Chain Reaction (Real-Time PCR) method (29 miRNAs). We evaluated the expression change of the target gene of miRNA, whose expression change was detected using in silico analysis, by q-RT PCR. Eight miRNAs with changes in expression were detected. Of these miRNAs, miR-342-p was downregulated in the PTZ group and was statistically significant (p < 0.005). Ultimately, we determined that the target gene of miR-342-p is a metabotropic glutamate receptor 2 (GRM2) and that GRM2 expression is upregulated. Conclusions Downregulation of miR-342-3p in the PTZ kindling model may result in the upregulation of GRM2.en_US
dc.description.sponsorshipResearch Fund of Bezmialem Vakif University [12.2014/15]en_US
dc.description.sponsorshipThe present work was funded by the Research Fund of Bezmialem Vakif University (grant number 12.2014/15).en_US
dc.identifier.doi10.1007/s11033-022-08017-5
dc.identifier.endpage12006en_US
dc.identifier.issn0301-4851
dc.identifier.issn1573-4978
dc.identifier.issue12en_US
dc.identifier.pmid36271980en_US
dc.identifier.startpage11997en_US
dc.identifier.urihttps://doi.org/10.1007/s11033-022-08017-5
dc.identifier.urihttps://hdl.handle.net/11616/102870
dc.identifier.volume49en_US
dc.identifier.wosWOS:000871174700001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofMolecular Biology Reportsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEpileptogenesisen_US
dc.subjectmiR-342-3pen_US
dc.subjectGRM2 geneen_US
dc.subjectHippocampusen_US
dc.subjectPentylenetetrazole kindling modelen_US
dc.titleDownregulatory effect of miR-342-3p on epileptogenesis in the PTZ-kindling modelen_US
dc.typeArticleen_US

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