The effect of lidocaine on TRPM 2,6,7 and 8 channels in liver ischemia / reperfusion model in rats

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dc.contributor.authorBilecik, Tuna
dc.contributor.authorOzozan, Omer Vefik
dc.contributor.authorCoke, Hasan
dc.date.accessioned2024-08-04T21:01:24Z
dc.date.available2024-08-04T21:01:24Z
dc.date.issued2020
dc.departmentİnönü Üniversitesien_US
dc.description.abstractAim: The transient receptor potential melastatin (TRPM) channel is subfamilies of the transient receptor potential (TRP) channels, cation permeable channels. Ischemia and reperfusion (I / R) injury is a phenomenon highlighting cellular damage of the liver caused by oxygen. Lidocaine is a local anesthetic that blocks sodium channels and suppresses mitochondrial functions of neutrophils. In this study, we purpose to investigate the effects of TRPM2/8 and TRPM6/7 expressions after lidocaine treatment in liver ischemia/reperfusion rat model. Materials and Methods: The study was carried out on 32 male Wistar rats. The animals were randomly divided into 4 groups including sham, lidocaine, I/R group and I/R- lidocaine group. The portal vein and the hepatic artery branches were clamped for 60 minutes for complete ischemia. TRPM2/8 and TRPM6/7 gene expression levels were assessed by RT-qPCR. Results: The expression levels of TRPM2 were significantly higher in liver-I/R group compared to sham and lidocaine groups (p<0.05, p<0.000, respectively), TRPM8 genes were significantly higher in liver-I/R group compared to sham and lidocaine groups (p<0.05, p<0.05, respectively). However, the expression levels of TRPM6 gene was significantly higher in liver-I/R group compared to sham, lidocaine, and I/R-lidocaine groups (p<0.05, p<0.01, p<0.05, respectively), TRPM7 genes was significantly higher in liver-I/R group compared to sham, lidocaine and I/R-lidocaine groups (p<0.05, p<0.000, p<0.05, respectively). Discussion: In conclusion, we firstly showed that an association between the expression level of TRPM2/8, TRPM6/7, and hepatic I/R, I/R-lidocaine groups as well as TRPM2/8 and TRPM6/7 gene expressions are affected by lidocaine in the liver-I/R in a rat model.en_US
dc.identifier.doi10.4328/ACAM.20075
dc.identifier.endpage324en_US
dc.identifier.issn2667-663X
dc.identifier.issue4en_US
dc.identifier.startpage319en_US
dc.identifier.urihttps://doi.org/10.4328/ACAM.20075
dc.identifier.urihttps://hdl.handle.net/11616/104353
dc.identifier.volume11en_US
dc.identifier.wosWOS:000572683500016en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherBayrakol Medical Publisheren_US
dc.relation.ispartofAnnals of Clinical and Analytical Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectSodium channelen_US
dc.subjectIschemiaen_US
dc.subjectReperfusionen_US
dc.subjectTRPMen_US
dc.subjectLidocaineen_US
dc.titleThe effect of lidocaine on TRPM 2,6,7 and 8 channels in liver ischemia / reperfusion model in ratsen_US
dc.typeArticleen_US

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