INVESTIGATING EFFECTS OF GRAPE SEED EXTRACT ON NEUROPATHIC PAIN IN THE STREPTOZOTOCIN-INDUCED DIABETIC MICE

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dc.authoridYildiz, Azibe/0000-0001-5686-7867
dc.authoridVardı, Nigar/0000-0003-0576-1696
dc.authoridGürbüz, Perihan/0000-0002-6632-9197
dc.authoridKOKSAL, Burcu/0000-0002-5840-3166
dc.authorwosidYildiz, Azibe/ABI-7998-2020
dc.authorwosidVardı, Nigar/C-9549-2018
dc.authorwosidGürbüz, Perihan/Y-4833-2018
dc.authorwosidKöksal, Burcu/JFK-5373-2023
dc.contributor.authorYurt, A.
dc.contributor.authorKoksal, B.
dc.contributor.authorGurbuz, P.
dc.contributor.authorYildiz, A.
dc.contributor.authorVardi, N.
dc.contributor.authorAlcin, E.
dc.date.accessioned2024-08-04T20:59:40Z
dc.date.available2024-08-04T20:59:40Z
dc.date.issued2019
dc.departmentİnönü Üniversitesien_US
dc.description.abstractDiabetes mellitus is a complicated and serious health problem involving peripheral neuropathy. This situation causes to loss of senses, tingle and pain. Diabetic peripheral neuropathy (DPN) affects 236 million people around the World. Hence there is a need to investigate alternative ways of cure focusing on DPN. Flavonoids have potential on pain due to their permeability characteristic in the capillary microcirculation system and in lowering blood pressure. Flavonoids are common in grape seed. The main flavonoids of grape seed involve proanthocyanidins which might be an effective agent in cure of pain in DPN. The purpose of this study is to investigate effects of grape seed extract on neuropathic pain in the Streptozotocin-induced diabetic mice. In the study, 50 eight-week old BALB-C strain mice in five different groups (Control, Diabetic Control, Control+25 mg/kg, Diabetes+25 mg/kg and Diabetes+50 mg/kg) were used. To induce diabetes in thirty of these animals, single dose Streptozotocin (180 mg/kg) was administered intraperitoneally. After diabetes was observed, grape seed extract (25 mg/kg and 50 mg/kg body weight) was administered by oral gavage in three groups (Control+25 mg/kg, Diabetes+25 mg/kg and Diabetes+50 mg/kg) during six weeks. At the end of the second and sixth weeks, pain threshold measurements in hot plate test were performed in line with a predetermined thermal pain model. Also the tissues of the sciatic nerve and abdominal aorta from the animals were histologically investigated. As a result hot plate measurements, pain threshold values of the animals in Diabetes+50mg/kg group significantly differed from the measurements of the animals in control group in the first measurements and from the animals in Diabetes+25 mg/kg group in the second measurements (p<0.05). However pain threshold values of the animals in Diabetes+25 mg/kg group differed significantly from the values of the animals in control+25 mg/kg group and control group. It means pain threshold values of the animals in Diabetes+50 mg/kg and Diabetes+25 mg/kg groups were significantly lower than the values of the animals in the other groups. The results of histological investigations showed that degenerations of myelin sheet and axons in diabetic control group were decreased significantly in Diabetes+50 mg/kg and Diabetes+25 mg/kg groups. Moreover degenerations of aorta tissues of animals in diabetic group were not seen in the animals of Diabetes+50 mg/kg except for tunica adventitia inflammation. It can be said that grape seed extract decreased threshold of neuropathic pain in the Streptozotocin-induced diabetic mice and prevented degenerations of myelin sheet and axons, and aorta tissues.en_US
dc.description.sponsorshipInonu University [2013/178]en_US
dc.description.sponsorshipThis study was supported by Inonu University Scientific Research Project Fund with the project number; 2013/178. Portions of this work were presented at the TFBD 41. National Physiology Congress (Canakkale, Turkey, 2015). This research did not receive any specific funding.en_US
dc.identifier.doi10.26641/2307-0404.2019.4.189197
dc.identifier.endpage58en_US
dc.identifier.issn2307-0404
dc.identifier.issue4en_US
dc.identifier.startpage51en_US
dc.identifier.urihttps://doi.org/10.26641/2307-0404.2019.4.189197
dc.identifier.urihttps://hdl.handle.net/11616/103424
dc.identifier.volume24en_US
dc.identifier.wosWOS:000504877900008en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherDnipropetrovsk State Medical Acaden_US
dc.relation.ispartofMedical Perspectives-Medicni Perspektivien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectdiabetes mellitusen_US
dc.subjectneuropathic painen_US
dc.subjectgrape seed extractionen_US
dc.subjecthot plateen_US
dc.subjectstreptozocin (STZ)en_US
dc.titleINVESTIGATING EFFECTS OF GRAPE SEED EXTRACT ON NEUROPATHIC PAIN IN THE STREPTOZOTOCIN-INDUCED DIABETIC MICEen_US
dc.typeArticleen_US

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