Morinda citrifolia protective effects on paclitaxel-induced testis parenchyma toxicity: An experimental study

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dc.authoridMAMOULAKIS, CHARALAMPOS/0000-0002-8662-1275
dc.authoridTsatsakis, Aristidis/0000-0003-3824-2462
dc.authorwosidMAMOULAKIS, CHARALAMPOS/HJH-2439-2023
dc.authorwosidTsatsakis, Aristidis/H-2890-2013
dc.contributor.authorGenc, Sidika
dc.contributor.authorCicek, Betul
dc.contributor.authorYeni, Yesim
dc.contributor.authorKuzucu, Mehmet
dc.contributor.authorHacimuftuoglu, Ahmet
dc.contributor.authorBolat, Ismail
dc.contributor.authorYildirim, Serkan
dc.date.accessioned2024-08-04T21:00:01Z
dc.date.available2024-08-04T21:00:01Z
dc.date.issued2024
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThe current study aimed to investigate the sensitivity of male testis parenchyma cells to chemotherapy agents and the protective effects and mechanisms of Morinda citrifolia (Noni) administration against structural and functional changes before and after chemotherapy (Paclitaxel (PTX)). For this purpose, rats were randomly assigned into four groups (Control = G1, PTX 5 mg/kg = G2; PTX + Noni 10 mg/kg = G3, PTX + Noni 20 mg/kg = G4). PTX was injected intraperitoneally for 4 consecutive weeks, at a dose of 5 mg/kg to all groups except the control group. Then noni was administrated in 10 (G3) and 20 (G4) mg/kg groups orally (gavage) for 14 days. Biochemical analyses, Real-Time Polymerase Chain Reaction (PCR), and immunohistochemical analyses were performed. According to our results, Total Oxidative Stress (TOS) and Malondialdehyde (MDA) were significantly increased in the PTX group (P < 0.01). Superoxide Dismutase (SOD) enzyme activity and Total Antioxidant Capacity (TAC) levels were decreased (P < 0.01). The changes in the rats treated with PTX + Noni 20 mg/ kg were noteworthy. The increased levels of IL1-beta (Interleukin 1 beta) and TNF alpha (tumor necrosis factor-alpha) with PTX were down-regulated after treatment with PTX + Noni 20 mg/kg (P < 0.01) (9 % and 5 % respectively). In addition, Noni restored the testicular histopathological structure by reducing caspase-3 expression and significantly (61 %) suppressed oxidative DNA damage and apoptosis (by regulating the Bax (bcl-2-like protein 4)/Bcl-2 (B-cell lymphoma gene-2) ratio). In conclusion, Noni reduced cellular apoptosis and drastically changed Caspase 8 and Bax/Bcl-2 levels. Furthermore, it considerably decreases oxidative damage and can be used in testicular degeneration.en_US
dc.identifier.doi10.1016/j.reprotox.2024.108611
dc.identifier.issn0890-6238
dc.identifier.issn1873-1708
dc.identifier.pmid38782144en_US
dc.identifier.urihttps://doi.org/10.1016/j.reprotox.2024.108611
dc.identifier.urihttps://hdl.handle.net/11616/103717
dc.identifier.volume127en_US
dc.identifier.wosWOS:001249219200001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherPergamon-Elsevier Science Ltden_US
dc.relation.ispartofReproductive Toxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectTestis parenchyma cellsen_US
dc.subjectNoni administrationen_US
dc.subjectOxidative stress and antioxidant levelsen_US
dc.subjectBaxen_US
dc.subjectBcl-2en_US
dc.subjectPaclitaxelen_US
dc.titleMorinda citrifolia protective effects on paclitaxel-induced testis parenchyma toxicity: An experimental studyen_US
dc.typeArticleen_US

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